Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 318,649 T→C 6.3% intergenic (‑82/+27) rclB ← / ← rclA reactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913318,6490TC 6.3% 426.1 / 13.8 142intergenic (‑82/+27)rclB/rclAreactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base T (63/70);  new base C (4/5);  total (67/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.25e-01

Only 100 of 146 total aligned reads displayed.

TCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATC  >  NC_000913/318500‑318796
                                                                                                                                                     |                                                                                                                                                   
 cTGCATTGGTGGAAAATGCCATAACGACAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAata                                                                                                                                                    >  1:557496/1‑150 (MQ=255)
 atGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAata                                                                                                                                                    <  2:540872/149‑1 (MQ=255)
    cATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATAttt                                                                                                                                                 >  2:338952/1‑150 (MQ=255)
    cATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATAttt                                                                                                                                                 >  2:282071/1‑150 (MQ=255)
    cATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATAttt                                                                                                                                                 <  1:809534/150‑1 (MQ=255)
     aTTGGTGGAAAATGCCATAACGCCAGTTAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTg                                                                                                                                                <  2:529258/150‑1 (MQ=255)
     aTTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTg                                                                                                                                                <  2:1239602/150‑1 (MQ=255)
         gtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCt                                                                                                                                            >  1:419113/1‑150 (MQ=255)
         gtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGCGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCt                                                                                                                                            >  1:423525/1‑150 (MQ=255)
           ggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTgg                                                                                                                                          >  2:1133832/1‑150 (MQ=255)
             aaaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCg                                                                                                                                        <  2:1610623/150‑1 (MQ=255)
             aaaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCg                                                                                                                                        <  1:742165/150‑1 (MQ=255)
             aaaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCg                                                                                                                                        <  1:866838/150‑1 (MQ=255)
               aaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGAGGCCAGAATATTTGTTCTGGCGTc                                                                                                                                      <  1:241521/150‑1 (MQ=255)
                   ccATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCGTCt                                                                                                                                     >  1:1274568/1‑147 (MQ=255)
                   ccATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCGTCt                                                                                                                                     <  2:1274568/147‑1 (MQ=255)
                   ccATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATAAAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGAt                                                                                                                                  >  2:1242895/1‑150 (MQ=255)
                    cATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGAtt                                                                                                                                 >  1:636225/1‑150 (MQ=255)
                    cATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGAtt                                                                                                                                 >  1:1581657/1‑150 (MQ=255)
                     aTAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGAttt                                                                                                                                <  2:363845/150‑1 (MQ=255)
                      tAACTCCATATTATATTGTTTCATATAAAATATTTTTTTTAAACATATAAAATACATTAAATGAATTTATTATGAATTTATAGATTAACGTAGAATTAATAATATTTTTTTCTTTTAGACTAATTAATATTTGTTCTGGCGTTTTAtttt                                                                                                                               <  2:154058/150‑1 (MQ=255)
                      tAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTATCTGAGACGCCAGAATATTTGTTCTGGCGTCTTAtttt                                                                                                                               >  1:868012/1‑150 (MQ=255)
                         cGCCAGATACTAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCTATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAg                                                                                                                            <  1:1114621/150‑1 (MQ=255)
                             aGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTa                                                                                                                        <  1:1788327/150‑1 (MQ=255)
                             aGATAATAGTGATGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTAATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTa                                                                                                                        <  2:89118/150‑1 (MQ=255)
                                taataGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAATTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTAttt                                                                                                                     <  2:1359025/150‑1 (MQ=255)
                                taataGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTAttt                                                                                                                     >  2:1767840/1‑150 (MQ=255)
                                 aataGTGTTTCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTg                                                                                                                    <  1:1373524/150‑1 (MQ=255)
                                 aataGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTg                                                                                                                    <  2:1008400/150‑1 (MQ=255)
                                 aataGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTg                                                                                                                    <  1:998639/150‑1 (MQ=255)
                                 aataGTGTTGAAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGATTTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTg                                                                                                                    >  2:107606/1‑150 (MQ=255)
                                        ttGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCTAAGATGTGATGAAGAAGTTATATAGTAACAGAGAATTAATAAGATTCTTTTATCTGAGACGCGAGAATATTTGTTCTGGAGTATGTTTATGAGTTTATTTGACTAATg                                                                                                             >  2:1710625/1‑150 (MQ=255)
                                          gCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAAAAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaa                                                                                                           >  1:1312776/1‑150 (MQ=255)
                                           cAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaaa                                                                                                          >  2:871161/1‑150 (MQ=255)
                                             aaTAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAAt                                                                                                        <  2:1637088/150‑1 (MQ=255)
                                               tAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAg                                                                                                      <  2:1710500/150‑1 (MQ=255)
                                                aaaaCAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGa                                                                                                     >  2:1474993/1‑150 (MQ=255)
                                                 aaaCAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGAt                                                                                                    >  2:399113/1‑150 (MQ=255)
                                                  aaCAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATc                                                                                                   <  2:619589/150‑1 (MQ=255)
                                                     aGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGACTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCAtt                                                                                                <  2:1191957/150‑1 (MQ=255)
                                                      taTTTTTTAAACATATAAAAAACCATCAATGATGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCAATAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTg                                                                                               <  2:624126/149‑1 (MQ=255)
                                                       aTTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGa                                                                                              >  2:331165/1‑150 (MQ=255)
                                                         tttttAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGt                                                                                            <  2:1585846/150‑1 (MQ=255)
                                                         tttttAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGt                                                                                            <  1:1141178/150‑1 (MQ=255)
                                                         tttttAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGt                                                                                            <  1:1293248/150‑1 (MQ=255)
                                                          ttttAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTg                                                                                           <  2:612832/150‑1 (MQ=255)
                                                           tttAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGa                                                                                          >  1:933054/1‑150 (MQ=255)
                                                             tAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGAtt                                                                                        >  2:1389249/1‑150 (MQ=255)
                                                                   tataAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCa                                                                                  >  1:1288429/1‑150 (MQ=255)
                                                                    ataAAATACCATCAATGAAGTGATTAAGAAGTTATATAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCAt                                                                                 <  2:754514/150‑1 (MQ=255)
                                                                     taAAATACCATCAATTTAGTGATTAAGAAGTTATAGAGTAACAGCGAATTAATAAGATGCTATTCTCTGAGCCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATc                                                                                <  2:284056/150‑1 (MQ=255)
                                                                     taAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATc                                                                                <  1:282071/150‑1 (MQ=255)
                                                                          taaaaTCAATGAAGTGATTAATAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGATAAGCCATAATTTTTGTTCTGTAGTCTGATTTTGAGTTTATTTGACTAATTAATATAGATCTTTGAGTGATTCTCTCATAGACgt                                                                           >  1:66184/5‑149 (MQ=255)
                                                                           aCCATCAATGAAGTGATTAAGAAGTTATAGAGTAAGAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGa                                                                          <  1:1619078/150‑1 (MQ=255)
                                                                             cATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                                        <  2:1800528/150‑1 (MQ=255)
                                                                             cATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                                        <  2:675182/150‑1 (MQ=255)
                                                                             cATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                                        >  1:1063939/1‑150 (MQ=255)
                                                                             cATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                                        <  1:651561/150‑1 (MQ=255)
                                                                             cATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                                        <  2:1254977/150‑1 (MQ=255)
                                                                             cATAAATGAAGTGATTAAGAAGTAATAGAGTAACAGAGACTTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                                        <  2:713830/150‑1 (MQ=255)
                                                                                 aaTGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTa                                                                    >  1:1520970/1‑150 (MQ=255)
                                                                                 aaTGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATAATTGAGTGATTCGCTCATCGACGGATGAGTa                                                                    >  1:924568/1‑150 (MQ=255)
                                                                                         gATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTg                                                            >  2:1480984/1‑150 (MQ=255)
                                                                                         gATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTg                                                            >  1:507534/1‑150 (MQ=255)
                                                                                           ttAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGATAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGAt                                                          >  1:827607/1‑150 (MQ=255)
                                                                                           ttAAGAAATTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGGTGAGTAAATATCTGAt                                                          >  1:1392596/1‑150 (MQ=255)
                                                                                            tAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTTAGTGATTCGCTCATCGACGGATGAGTAAATATCTGAta                                                         >  2:1168773/1‑149 (MQ=255)
                                                                                              agaagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGAGATTCGCTCATCGACGGATGAGTAAATATCTGATcgc                                                       >  1:1549350/1‑150 (MQ=255)
                                                                                               gaagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTGCTGGCGTCTGAGTTTGAGTGTATTTGCCTACTGAAAATAGCTCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATcgcg                                                      <  2:1596016/150‑1 (MQ=255)
                                                                                                   ttATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAat                                                  >  2:518977/1‑150 (MQ=255)
                                                                                                    tataGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGAGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAata                                                 >  1:1608171/1‑150 (MQ=255)
                                                                                                      taGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATg                                               >  2:666804/1‑150 (MQ=255)
                                                                                                       agagTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGc                                              <  1:1690533/150‑1 (MQ=255)
                                                                                                        tagTAACAGATAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCt                                             <  2:1809255/149‑1 (MQ=255)
                                                                                                        gagTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCt                                             >  1:390798/1‑150 (MQ=255)
                                                                                                              cAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGACTAAATATCTGATCGCGTAATATGCTATAAgg                                       <  2:433020/150‑1 (MQ=255)
                                                                                                               agagAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCTACGGATGAGTAAATATCTGATCGCGTAATATTCTATAAGGc                                      >  1:260969/1‑150 (MQ=255)
                                                                                                               agagAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGc                                      <  2:1582357/150‑1 (MQ=255)
                                                                                                               agagAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGc                                      <  1:331165/150‑1 (MQ=255)
                                                                                                                  gaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGc                                   >  2:221219/1‑150 (MQ=255)
                                                                                                                     ttAATAAGATTCTTTTCTCTGAGACGCCAGATTATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCg                                >  1:33826/1‑150 (MQ=255)
                                                                                                                       aataaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTTTAAGGCAGCCCGGc                              >  2:1766838/1‑150 (MQ=255)
                                                                                                                        ataaGATTCTTTTCTCTTAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGca                             <  1:1050742/150‑1 (MQ=255)
                                                                                                                             aTTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCTCGTAATATGCTATAAGGCAGCCCGGcatccat                        >  1:301476/1‑150 (MQ=255)
                                                                                                                                 ttttCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAcc                    >  2:1320495/1‑150 (MQ=255)
                                                                                                                                    tctcTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCAtt                 <  2:1826090/150‑1 (MQ=255)
                                                                                                                                     ctctGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCAttt                >  2:1518309/1‑150 (MQ=255)
                                                                                                                                       ctGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTc              >  2:1623061/1‑150 (MQ=255)
                                                                                                                                        tGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCa             >  2:448003/1‑150 (MQ=255)
                                                                                                                                        tGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCa             >  1:1413392/1‑150 (MQ=255)
                                                                                                                                        tGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGAACGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCa             <  2:419113/150‑1 (MQ=255)
                                                                                                                                        tGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCGCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCa             <  2:1075396/150‑1 (MQ=255)
                                                                                                                                         gattcggcAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCAc            <  2:594912/143‑1 (MQ=255)
                                                                                                                                         gagaCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCAc            >  2:76825/1‑150 (MQ=255)
                                                                                                                                          agaCGGCAGAATATTTGTTCTGGCGTGTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACt           <  2:72971/150‑1 (MQ=255)
                                                                                                                                           gaCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCTACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACta          <  1:874854/150‑1 (MQ=255)
                                                                                                                                           gaCGCCAGAACAAATATTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGATTAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCCTCCATCACCATTTTCACgat         >  2:1191261/1‑150 (MQ=255)
                                                                                                                                            aCGCCAGAACAAATATTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACtat         >  2:1657984/1‑150 (MQ=255)
                                                                                                                                               ccAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATAtt      >  2:904346/1‑150 (MQ=255)
                                                                                                                                                 aGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGa    >  2:841913/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCTGCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATC  >  NC_000913/318500‑318796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: