BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,142,505	G→C	5.3%	R148R (CGC→CGG)	udk ←	uridine/cytidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,142,505	0	G	C	5.3%	422.3 / 12.4	131	R148R (CGC→CGG)	udk	uridine/cytidine kinase
Reads supporting (aligned to +/- strand): ref base G (61/63); new base C (4/3); total (65/66)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

Only 100 of 138 total aligned reads displayed.

CGGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTAT  >  NC_000913/2142356‑2142650

cGGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATgcgg                                                                                                                                                   >  2:1368038/1‑150 (MQ=255)

 ggTTTTTCCGGCCGCGCGGCACGATAATGTCCTCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATgcggc                                                                                                                                                  <  2:730380/150‑1 (MQ=255)

 ggTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATgcggc                                                                                                                                                  >  2:393192/1‑150 (MQ=255)

 ggTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATgcggc                                                                                                                                                  >  2:10737/1‑150 (MQ=255)

 ggTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATgcggc                                                                                                                                                  <  1:1515190/150‑1 (MQ=255)

 ggTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATgcggc                                                                                                                                                  >  1:546893/1‑150 (MQ=255)

    ttttCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCa                                                                                                                                               <  1:700984/150‑1 (MQ=255)

         ccgccgCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAgg                                                                                                                                          <  1:1129905/150‑1 (MQ=255)

         ccgccgCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAgg                                                                                                                                          >  1:1587866/1‑150 (MQ=255)

         ccgccgCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAgg                                                                                                                                          >  2:135511/1‑150 (MQ=255)

          cgccgcGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGc                                                                                                                                         <  2:453284/150‑1 (MQ=255)

           gccgcGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCa                                                                                                                                        <  2:18421/150‑1 (MQ=255)

             cgcgcgGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGa                                                                                                                                      <  1:68211/150‑1 (MQ=255)

             cgcgcgGCACGATAATGTCAGCATATTGTTTAGAAGGCTCAATGAATTGCATGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGa                                                                                                                                      <  2:671926/150‑1 (MQ=255)

              gcgcgGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGTAACATCGGGCTCACGGGTTTTTGATATTGCGCCAGCACTGAATCCATTGAACGCCCACGCGCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGat                                                                                                                                     <  1:1234052/150‑1 (MQ=255)

                 cgGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATc                                                                                                                                  >  2:551463/1‑150 (MQ=255)

                      cGATAATGCCCGCATATTGTTTAGAAGGCTCAATGAATAGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCg                                                                                                                             <  2:649010/150‑1 (MQ=255)

                       gATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCgg                                                                                                                            <  2:1527838/150‑1 (MQ=255)

                       gATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCgg                                                                                                                            <  1:979226/150‑1 (MQ=255)

                       gATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCgg                                                                                                                            <  1:972896/150‑1 (MQ=255)

                        aTAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCggg                                                                                                                           <  1:430090/150‑1 (MQ=255)

                            tGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGATTTTTGATATTGCGACATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTAt                                                                                                                       >  1:1214961/1‑150 (MQ=255)

                                  cATATTGTTTAGCAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCCCGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGa                                                                                                                 >  1:314210/1‑150 (MQ=255)

                                  cATATTGTTTAGAAGGCTCAATGTATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGa                                                                                                                 <  1:1008710/150‑1 (MQ=255)

                                   atatTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCAATGAACGCCCACGCTCCTTAACTTCACGCTTGATGCGGAGCATCAGGCAGATATACAGCTGGGTATCAACGaa                                                                                                                >  2:799730/1‑150 (MQ=255)

                                      ttGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAAt                                                                                                             >  1:1194646/1‑150 (MQ=255)

                                        ggttATAAGGCGCAAAGAATTGCAGGAAAATAGGGCGCACCGGTTTTTGATATTTCGCCATCACAGAATACATTGGACGCCCCCGCGAGTTAACGTCACGCTTGATGCGGCGCCTCAGGCAGATATGCCGCGGGGGTTCAACGAAAATgg                                                                                                           >  2:1808357/3‑150 (MQ=255)

                                        gTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATgg                                                                                                           <  2:655374/150‑1 (MQ=255)

                                         tttAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGga                                                                                                          >  1:1679821/1‑150 (MQ=255)

                                         tttAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGga                                                                                                          >  1:176368/1‑150 (MQ=255)

                                         tttAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGga                                                                                                          >  2:508816/1‑150 (MQ=255)

                                         tttAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCAAGCGGGGTATCAACGAAAATGga                                                                                                          >  2:1724390/1‑150 (MQ=255)

                                           tAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGgaga                                                                                                        >  2:1270586/1‑150 (MQ=255)

                                           tAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGgaga                                                                                                        >  2:396173/1‑150 (MQ=255)

                                             taaGGCACAATGAATTGCATGAACAGCGGGCGCACGGTTTTTTGATCTTGCTCCATCACTGAATCCATTGAACGCACACGCTCGTTAACGTCACGCTTGAGGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaag                                                                                                      <  2:72581/149‑1 (MQ=255)

                                             gAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaag                                                                                                      <  2:3697/150‑1 (MQ=255)

                                             gAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaag                                                                                                      >  1:457549/1‑150 (MQ=255)

                                                ggCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATTTTGCGCCATCACTGAATCCAATGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaagtta                                                                                                   <  1:325844/150‑1 (MQ=255)

                                                 gCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaagttaa                                                                                                  >  1:1432500/1‑150 (MQ=255)

                                                  cTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaagttaag                                                                                                 <  2:1675528/150‑1 (MQ=255)

                                                    cAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaagttaagtt                                                                                               <  1:34564/150‑1 (MQ=255)

                                                     aaTGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTc                                                                                              <  1:463650/150‑1 (MQ=255)

                                                      aTGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCg                                                                                             <  1:551463/150‑1 (MQ=255)

                                                        taaTTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTc                                                                                           <  2:20972/149‑1 (MQ=255)

                                                         aaTTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCa                                                                                          <  1:1793278/150‑1 (MQ=255)

                                                            tGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGc                                                                                       >  2:250260/1‑150 (MQ=255)

                                                             gCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCa                                                                                      >  2:1228289/1‑150 (MQ=255)

                                                             gCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCa                                                                                      <  1:250655/150‑1 (MQ=255)

                                                              cAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCaa                                                                                     >  2:897988/1‑150 (MQ=255)

                                                              cAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCAGCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCaa                                                                                     >  1:113127/1‑150 (MQ=255)

                                                                ggAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAc                                                                                   >  2:1612878/1‑150 (MQ=255)

                                                                ggAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAc                                                                                   >  2:1529691/1‑150 (MQ=255)

                                                                ggAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAc                                                                                   >  2:796719/1‑150 (MQ=255)

                                                                ggAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAc                                                                                   >  1:693637/1‑150 (MQ=255)

                                                                ggAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAc                                                                                   >  1:1301254/1‑150 (MQ=255)

                                                                    cATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGGATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACgcgc                                                                               >  2:144176/1‑150 (MQ=255)

                                                                         ggCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCg                                                                          >  1:1499826/1‑150 (MQ=255)

                                                                                ggTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGcaa                                                                   >  1:1797245/1‑150 (MQ=255)

                                                                                    ttttGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACaaa                                                               >  1:933489/1‑150 (MQ=255)

                                                                                       tGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATg                                                            <  1:2180/150‑1 (MQ=255)

                                                                                           atTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCtt                                                        <  2:497898/150‑1 (MQ=255)

                                                                                             tGCGCCATCACTGAATCCCTTGACCGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCg                                                      <  2:294652/150‑1 (MQ=255)

                                                                                                 ccATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGaa                                                  <  1:1228289/150‑1 (MQ=255)

                                                                                                  cATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAat                                                 <  1:1314950/150‑1 (MQ=255)

                                                                                                  cATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAat                                                 <  2:116381/150‑1 (MQ=255)

                                                                                                   aTCACTGAATCCCAGGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCAGCCGTCCGCAACAAAATGCCTTCGAGAatg                                                <  2:879062/150‑1 (MQ=255)

                                                                                                   aTCACTGAATCCATTGAACTGACAGGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTATGTTCGTCACGCAAACGCGCTTCCGTCAGCAACAAAATGCCTTCGAGAttg                                                <  1:88019/150‑1 (MQ=255)

                                                                                                      aCTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatgatg                                             >  2:599954/1‑150 (MQ=255)

                                                                                                      aCTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatgatg                                             <  1:1598801/150‑1 (MQ=255)

                                                                                                      aCTGAATCCATTGAACGCCCACGCTCGTTAACGTCAAGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatgatg                                             <  1:1591831/150‑1 (MQ=255)

                                                                                                         taaTCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGAcc                                          <  1:135511/149‑1 (MQ=255)

                                                                                                          aaTCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACct                                         >  1:869518/1‑150 (MQ=255)

                                                                                                           aTCCATTGAACGCCCACGCTCGTTAACGTCACGTTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACctt                                        >  2:1190154/1‑150 (MQ=255)

                                                                                                            tCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCCCGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACcttc                                       >  1:1631139/1‑150 (MQ=255)

                                                                                                              cATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACcttctt                                     >  2:1131498/1‑150 (MQ=255)

                                                                                                                 tGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCgg                                  <  1:508816/150‑1 (MQ=255)

                                                                                                                 tGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCAAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCgg                                  <  1:1724390/150‑1 (MQ=255)

                                                                                                                  gAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGc                                 <  2:279705/150‑1 (MQ=255)

                                                                                                                  caaCGCCCACGCTCGTTAACGTCACGCTAGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGc                                 <  2:1610464/149‑1 (MQ=255)

                                                                                                                   aaCGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCATATATCCAGCGGGGTATCAACGAAACTGGATAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCt                                <  2:1370305/150‑1 (MQ=255)

                                                                                                                    aCGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTc                               <  2:1169265/150‑1 (MQ=255)

                                                                                                                     cGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCACCAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCa                              >  2:38414/1‑150 (MQ=255)

                                                                                                                     cGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCa                              >  2:1482100/1‑150 (MQ=255)

                                                                                                                      gCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCaa                             >  1:306499/1‑150 (MQ=255)

                                                                                                                           cGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTc                        >  2:239865/1‑150 (MQ=255)

                                                                                                                                   aaCGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttc                <  1:42497/150‑1 (MQ=255)

                                                                                                                                     cGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttctt              <  1:1251782/150‑1 (MQ=255)

                                                                                                                                       tCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttctttc            <  2:1679821/150‑1 (MQ=255)

                                                                                                                                        cACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCa           >  2:573902/1‑150 (MQ=255)

                                                                                                                                        cACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCa           <  1:1368038/150‑1 (MQ=255)

                                                                                                                                        cACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTACGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCa           <  2:1432500/150‑1 (MQ=255)

                                                                                                                                          cGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATa         <  1:1626751/150‑1 (MQ=255)

                                                                                                                                          cGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATa         >  1:802613/1‑150 (MQ=255)

                                                                                                                                           gcctGATGCGCCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAc        >  2:1015548/4‑150 (MQ=255)

                                                                                                                                           gcctGATGCGCCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAc        >  2:378038/4‑150 (MQ=255)

                                                                                                                                              tGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgcg     <  2:707745/150‑1 (MQ=255)

                                                                                                                                              tGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgcg     >  1:1399775/1‑150 (MQ=255)

                                                                                                                                                aTGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTa   <  1:796719/150‑1 (MQ=255)

                                                                                                                                                 tGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTAt  >  2:1640300/1‑150 (MQ=255)

                                                                                                                                                 tGCGGCGCATCAGGAAGATATCCAGCGGGGTATCAACGAAAATGGAGCAGTTAAGTTCGTCACGCAAACGCGCAGCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGGCACCGTTTCTTTCATACGCGTAt  >  1:1644060/1‑150 (MQ=255)

CGGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTAT  >  NC_000913/2142356‑2142650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`