Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,153,396 C→A 6.7% noncoding (88/143 nt)
V5F (GTC→TTC) 
sibA →
ibsA ←
sRNA antisense regulator of toxic IbsA protein
toxic membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,3960CA 6.7% 305.2 / 4.2 105noncoding (88/143 nt)
V5F (GTC→TTC) 
sibA
ibsA
sRNA antisense regulator of toxic IbsA protein
toxic membrane protein
Reads supporting (aligned to +/- strand):  ref base C (47/49);  new base A (6/3);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TCGCCTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTG  >  NC_000913/2153247‑2153545
                                                                                                                                                     |                                                                                                                                                     
tCGCCTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGAc                                                                                                                                                       >  1:1556325/1‑150 (MQ=255)
 caccTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACg                                                                                                                                                      >  1:839489/3‑150 (MQ=255)
 cGCCTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACg                                                                                                                                                      >  1:514156/1‑150 (MQ=255)
    cTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGc                                                                                                                                                   <  1:1196537/150‑1 (MQ=255)
      cGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTGTTAGTAAGCGTGGAAGATAATCAGTAAAATGATCTCCACTAAGCTGAAGTTCtt                                                                                                                                                 >  1:347898/1‑150 (MQ=255)
       gCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtc                                                                                                                                                >  1:1302354/1‑150 (MQ=255)
        cAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtca                                                                                                                                               >  1:821346/1‑150 (MQ=255)
        cAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtca                                                                                                                                               <  1:1339949/150‑1 (MQ=255)
          aGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtcatc                                                                                                                                             >  2:1099591/1‑150 (MQ=255)
          aGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtcatc                                                                                                                                             <  2:802963/150‑1 (MQ=255)
               ggAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGAAGCACGTCATCATact                                                                                                                                        <  2:375133/150‑3 (MQ=255)
                 agagCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAAccc                                                                                                                                      <  2:723902/150‑1 (MQ=255)
                     ccGTCGTTTGACACTAATGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGGTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACAAGTATGATGACGTGCTTCATCATAACCCTTTc                                                                                                                                  <  2:900088/150‑1 (MQ=255)
                     ccGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTc                                                                                                                                  >  2:301235/1‑150 (MQ=255)
                      cGTCGATTGACACTAAGGGCGGAGTGACATAACTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTcc                                                                                                                                 >  1:510433/1‑150 (MQ=255)
                            ttGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCttatta                                                                                                                           <  2:970963/150‑1 (MQ=255)
                             tGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTaa                                                                                                                          <  2:1731871/150‑1 (MQ=255)
                              gACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGTTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGAAGTGCTTCATCATAACCCATTCATTATTaaa                                                                                                                         >  2:901321/1‑150 (MQ=255)
                              gACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTaaa                                                                                                                         <  2:1586908/150‑1 (MQ=255)
                              gACACTAAGGGCGGAGTGACATAAATTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCGGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCCCCAGTATGATGACGTGCTTCATCATAACCATTTCCGTATTaaa                                                                                                                         >  2:270727/1‑150 (MQ=255)
                               acacTAAGGGCGGAGTGACATAATATCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTATTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGAGCTTCATCATAACCCTTACCTAATTaaaa                                                                                                                        >  2:1547844/1‑150 (MQ=39)
                                cacTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGATCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAg                                                                                                                       <  1:123798/150‑1 (MQ=255)
                                  cTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGATAATCACTAAGAGTATAACCAGTATGATGAAGTGCTTCATCATAACCATTTCATTATTAAAAGcc                                                                                                                     >  2:1721029/1‑150 (MQ=255)
                                                   tAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAgg                                                                                                    >  1:1304001/1‑150 (MQ=255)
                                                    aaTTTCTGGAGTGAGGGTTAGCGAGAGGGTTCTCCCTCCCCCTTGTGTGCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGTCGTGCTTCTTCTTAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGc                                                                                                   <  1:1693323/150‑1 (MQ=255)
                                                    aaTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCATGGAAGCTAATCACTAAGACTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGc                                                                                                   >  2:946545/1‑150 (MQ=255)
                                                     aTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGAATCACCAGTATGATGACGTGCTTCATCATAACACTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGcg                                                                                                  >  2:1585234/1‑149 (MQ=255)
                                                     aTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTATTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCCTCATAAACCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCt                                                                                                  >  1:1013357/1‑150 (MQ=255)
                                                      tttCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCtt                                                                                                 >  2:964805/1‑150 (MQ=255)
                                                         catgAGTGAGGGTTAGGGAGAGGTTTCCCCCTCAACATGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAATAGTATCACCAGTATGATGACGTGCTTCATCATAGCCCTTTCATTATTAAAAGCCCTCTTCTCAGGGAGAGGCTTTca                                                                                              >  1:1014773/4‑149 (MQ=255)
                                                         cAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTcc                                                                                              >  2:6142/1‑150 (MQ=255)
                                                          aGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTccc                                                                                             <  1:1674373/150‑1 (MQ=255)
                                                            gAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGt                                                                                           <  2:827673/150‑1 (MQ=255)
                                                             aGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGtt                                                                                          >  2:1250316/1‑150 (MQ=255)
                                                             aGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGtt                                                                                          <  1:585578/150‑1 (MQ=255)
                                                              gTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGttt                                                                                         <  2:1073423/150‑1 (MQ=255)
                                                               ggAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTc                                                                                        <  2:55617/149‑1 (MQ=255)
                                                                  gggTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGc                                                                                     <  1:1512202/150‑1 (MQ=255)
                                                                   ggTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCg                                                                                    >  1:436367/1‑150 (MQ=255)
                                                                      tAGGGAGATGTTTACCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTcc                                                                                 <  2:1300429/150‑1 (MQ=255)
                                                                       aGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTccc                                                                                >  1:1480777/1‑150 (MQ=255)
                                                                       aGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTccc                                                                                <  2:1056641/150‑1 (MQ=255)
                                                                         ggAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTTATCACTAAGAGGATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCAAAGCTCCGGGAGCGCCTTTAAAGATTCAGCGTCCAGc                                                                              >  2:774769/1‑150 (MQ=37)
                                                                         ggAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGc                                                                              >  2:1812730/1‑150 (MQ=255)
                                                                           agagGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTg                                                                            <  1:964805/150‑1 (MQ=255)
                                                                             agGTTTCCCCCTCCCACGGGGGTTCTTAGTAAGCCTGGAAGCTAATCACTAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTACCTTATTAAAATCCATCTTCTCAGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                         >  2:797451/1‑150 (MQ=37)
                                                                              ggTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                         >  2:1019226/1‑150 (MQ=255)
                                                                              ggTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                         >  2:665802/1‑150 (MQ=255)
                                                                               ggttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaac                                                                        <  2:225302/148‑2 (MQ=255)
                                                                               gTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaac                                                                        <  2:790455/150‑2 (MQ=255)
                                                                                tttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAata                                                                       >  2:408395/1‑149 (MQ=255)
                                                                                 ttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                      <  1:702059/150‑4 (MQ=255)
                                                                                     ccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCgg                                                                  <  1:113897/150‑1 (MQ=255)
                                                                                         ccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTa                                                              <  2:510433/150‑1 (MQ=255)
                                                                                           ccctGGTGTTCTTAGTAAGCCTTGAATCTAATCACTAAGAGTATCACCAGTATGATGACGGGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCTGCTTAcc                                                            <  1:1642580/150‑1 (MQ=255)
                                                                                             ctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcctc                                                          <  1:1637349/150‑1 (MQ=255)
                                                                                              tGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcctcc                                                         <  2:1556325/150‑1 (MQ=255)
                                                                                                gtgtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCtt                                                       <  2:54429/150‑1 (MQ=255)
                                                                                                  gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                     >  2:658193/1‑150 (MQ=255)
                                                                                                  gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                     >  1:707618/1‑150 (MQ=255)
                                                                                                  gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                     >  2:1509676/1‑150 (MQ=255)
                                                                                                    tCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGc                                                   >  1:1473086/1‑150 (MQ=255)
                                                                                                     cTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCggc                                                  <  1:251666/150‑3 (MQ=255)
                                                                                                      ttAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGccc                                                 <  1:1679496/150‑1 (MQ=255)
                                                                                                       tAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCTTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCt                                                >  1:1695899/1‑150 (MQ=255)
                                                                                                       tAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTACAGCGTCCCGCTGAAATCATCGGCTTACATCCTTTCGCCCt                                                >  1:1385995/1‑150 (MQ=255)
                                                                                                          tAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGaa                                             <  2:521030/150‑1 (MQ=255)
                                                                                                          tAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGaa                                             <  1:873899/150‑1 (MQ=255)
                                                                                                           aaGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAAt                                            >  1:1293307/1‑150 (MQ=255)
                                                                                                              ccTGGAAGATAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                         <  1:1501667/150‑1 (MQ=255)
                                                                                                                tGGAAGCTAATCACTAAGACTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGATGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGt                                       >  2:837421/1‑150 (MQ=255)
                                                                                                                    aGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTACTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCCCTGAAAACATCGGATTACCTCCTTACCCCATGAAACCAGTCTaa                                   >  2:129296/1‑149 (MQ=25)
                                                                                                                     gCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATc                                  <  1:746104/150‑1 (MQ=255)
                                                                                                                      cTAATCACTACGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 <  2:121779/150‑1 (MQ=255)
                                                                                                                      cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 <  1:782561/150‑1 (MQ=255)
                                                                                                                      cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 <  2:839489/150‑1 (MQ=255)
                                                                                                                      cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 >  1:490773/1‑150 (MQ=255)
                                                                                                                      cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 <  1:1407057/150‑1 (MQ=255)
                                                                                                                       tAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGc                                <  1:1812730/150‑1 (MQ=255)
                                                                                                                           cACTAAGCGTAGCACCAGTAGGATGACGTTCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTCCCTCCTTTCGCCCTGAAGGCAGTCTATCGCTAAc                            <  2:266688/150‑1 (MQ=21)
                                                                                                                            aCTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGATGAAATCTTCGGCTTACCTCATTTCGCCATGAATGCAGTctaacgctaacg                           >  1:212489/1‑150 (MQ=21)
                                                                                                                             cTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATAGGCTTACCTACTTTCGCCCTGAATGCAGTCTATCGCTAAcgc                          >  1:1380375/1‑150 (MQ=255)
                                                                                                                              tAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCTTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAAcgcg                         <  2:428238/150‑1 (MQ=255)
                                                                                                                              tAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAAcgcg                         >  2:944900/1‑150 (MQ=255)
                                                                                                                               aaGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGt                        <  2:1334604/150‑1 (MQ=255)
                                                                                                                                agagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTg                       >  1:435465/1‑150 (MQ=255)
                                                                                                                                      tCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTACCGCTGAAATCATAGGCTTACCTCAGTTCGCCCTGATTGCAGTGTATCGCTAACGCGTGAATaat                 >  1:739076/1‑149 (MQ=37)
                                                                                                                                       cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                >  2:611563/1‑150 (MQ=255)
                                                                                                                                       cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCATGAATGCAGTATATCGCTAACGCGTGAATaaaa                >  2:411484/1‑150 (MQ=255)
                                                                                                                                         ccAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATa              >  2:354497/1‑150 (MQ=255)
                                                                                                                                          ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                <  2:597940/144‑1 (MQ=21)
                                                                                                                                          ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                <  2:1336172/144‑1 (MQ=21)
                                                                                                                                          ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                >  1:1336172/4‑147 (MQ=21)
                                                                                                                                          ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                >  1:597940/4‑147 (MQ=21)
                                                                                                                                          ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTACAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAg             >  1:973547/4‑150 (MQ=21)
                                                                                                                                             tATGATGACGTGCTTCATCATATCCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGGTTTCCCGTTTCAGCGTCCCGCTGAAATCTTCGTCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGttt          <  1:270727/150‑1 (MQ=255)
                                                                                                                                                gatgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCct       <  2:821346/150‑1 (MQ=255)
                                                                                                                                                 atgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCctc      >  2:1472884/1‑150 (MQ=255)
                                                                                                                                                  tgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCctct     <  1:1099591/150‑1 (MQ=255)
                                                                                                                                                     cGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCtgtg  >  1:348428/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGCCTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTG  >  NC_000913/2153247‑2153545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: