Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,153,398 T→C 5.9% noncoding (90/143 nt)
H4R (CAC→CGC) ‡
sibA →
ibsA ←
sRNA antisense regulator of toxic IbsA protein
toxic membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,3980TC 5.9% 309.1 / 7.4 104noncoding (90/143 nt)
H4R (CAC→CGC) ‡
sibA
ibsA
sRNA antisense regulator of toxic IbsA protein
toxic membrane protein
Reads supporting (aligned to +/- strand):  ref base T (48/48);  new base C (3/3);  total (52/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 104 total aligned reads displayed.

CTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTG  >  NC_000913/2153251‑2153545
                                                                                                                                                   |                                                                                                                                                   
cTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGc                                                                                                                                                   <  1:1196537/150‑1 (MQ=255)
  cGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTGTTAGTAAGCGTGGAAGATAATCAGTAAAATGATCTCCACTAAGCTGAAGTTCtt                                                                                                                                                 >  1:347898/1‑150 (MQ=255)
   gCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtc                                                                                                                                                >  1:1302354/1‑150 (MQ=255)
    cAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtca                                                                                                                                               >  1:821346/1‑150 (MQ=255)
    cAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtca                                                                                                                                               <  1:1339949/150‑1 (MQ=255)
      aGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtcatc                                                                                                                                             <  2:802963/150‑1 (MQ=255)
           ggAGAGCCGTCGATTGAGACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACAAAGAGTATCACCAGTATGATGACGTGCTTCATCATAAc                                                                                                                                        <  1:280079/150‑1 (MQ=255)
           ggAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGAAGCACGTCATCATact                                                                                                                                        <  2:375133/150‑3 (MQ=255)
             agagCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAAccc                                                                                                                                      <  2:723902/150‑1 (MQ=255)
                 ccGTCGTTTGACACTAATGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGGTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACAAGTATGATGACGTGCTTCATCATAACCCTTTc                                                                                                                                  <  2:900088/150‑1 (MQ=255)
                 ccGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTc                                                                                                                                  >  2:301235/1‑150 (MQ=255)
                  cGTCGATTGACACTAAGGGCGGAGTGACATAACTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTcc                                                                                                                                 >  1:510433/1‑150 (MQ=255)
                        ttGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCttatta                                                                                                                           <  2:970963/150‑1 (MQ=255)
                         tGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTaa                                                                                                                          <  2:1731871/150‑1 (MQ=255)
                          gACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGTTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGAAGTGCTTCATCATAACCCATTCATTATTaaa                                                                                                                         >  2:901321/1‑150 (MQ=255)
                          gACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTaaa                                                                                                                         <  2:1586908/150‑1 (MQ=255)
                          gACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATAATAACCCTTTCCTTATTaaa                                                                                                                         >  1:1475442/1‑150 (MQ=255)
                          gACACTAAGGGCGGAGTGACATAAATTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCGGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCCCCAGTATGATGACGTGCTTCATCATAACCATTTCCGTATTaaa                                                                                                                         >  2:270727/1‑150 (MQ=255)
                           acacTAAGGGCGGAGTGACATAATATCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTATTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGAGCTTCATCATAACCCTTACCTAATTaaaa                                                                                                                        >  2:1547844/1‑150 (MQ=39)
                            cacTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGATCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAg                                                                                                                       <  1:123798/150‑1 (MQ=255)
                              cTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGATAATCACTAAGAGTATAACCAGTATGATGAAGTGCTTCATCATAACCATTTCATTATTAAAAGcc                                                                                                                     >  2:1721029/1‑150 (MQ=255)
                                               tAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAgg                                                                                                    >  1:1304001/1‑150 (MQ=255)
                                                aaTTTCTGGAGTGAGGGTTAGCGAGAGGGTTCTCCCTCCCCCTTGTGTGCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGTCGTGCTTCTTCTTAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGc                                                                                                   <  1:1693323/150‑1 (MQ=255)
                                                aaTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCATGGAAGCTAATCACTAAGACTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGc                                                                                                   >  2:946545/1‑150 (MQ=255)
                                                 aTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGAATCACCAGTATGATGACGTGCTTCATCATAACACTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGcg                                                                                                  >  2:1585234/1‑149 (MQ=255)
                                                 aTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTATTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCCTCATAAACCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCt                                                                                                  >  1:1013357/1‑150 (MQ=255)
                                                  tttCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCtt                                                                                                 >  2:964805/1‑150 (MQ=255)
                                                     catgAGTGAGGGTTAGGGAGAGGTTTCCCCCTCAACATGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAATAGTATCACCAGTATGATGACGTGCTTCATCATAGCCCTTTCATTATTAAAAGCCCTCTTCTCAGGGAGAGGCTTTca                                                                                              >  1:1014773/4‑149 (MQ=255)
                                                     cAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTcc                                                                                              >  2:6142/1‑150 (MQ=255)
                                                      aGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTccc                                                                                             <  1:1674373/150‑1 (MQ=255)
                                                        gAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGt                                                                                           <  2:827673/150‑1 (MQ=255)
                                                         aGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGtt                                                                                          >  2:1250316/1‑150 (MQ=255)
                                                         aGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGtt                                                                                          <  1:585578/150‑1 (MQ=255)
                                                          gTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGttt                                                                                         <  2:1073423/150‑1 (MQ=255)
                                                           ggAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTc                                                                                        <  2:55617/149‑1 (MQ=255)
                                                              gggTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGc                                                                                     <  1:1512202/150‑1 (MQ=255)
                                                               ggTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCg                                                                                    >  1:436367/1‑150 (MQ=255)
                                                                  tAGGGAGATGTTTACCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTcc                                                                                 <  2:1300429/150‑1 (MQ=255)
                                                                   aGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTccc                                                                                >  1:1480777/1‑150 (MQ=255)
                                                                   aGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTccc                                                                                <  2:1056641/150‑1 (MQ=255)
                                                                     ggAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTTATCACTAAGAGGATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCAAAGCTCCGGGAGCGCCTTTAAAGATTCAGCGTCCAGc                                                                              >  2:774769/1‑150 (MQ=37)
                                                                     ggAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGc                                                                              >  2:1812730/1‑150 (MQ=255)
                                                                       agagGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTg                                                                            <  1:964805/150‑1 (MQ=255)
                                                                         agGTTTCCCCCTCCCACGGGGGTTCTTAGTAAGCCTGGAAGCTAATCACTAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTACCTTATTAAAATCCATCTTCTCAGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                         >  2:797451/1‑150 (MQ=37)
                                                                          ggTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                         >  2:665802/1‑150 (MQ=255)
                                                                          ggTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                         >  2:1019226/1‑150 (MQ=255)
                                                                           ggttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaac                                                                        <  2:225302/148‑2 (MQ=255)
                                                                           gTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaac                                                                        <  2:790455/150‑2 (MQ=255)
                                                                            tttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAata                                                                       >  2:408395/1‑149 (MQ=255)
                                                                             ttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                      <  1:702059/150‑4 (MQ=255)
                                                                               ccccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAatcatc                                                                    <  2:765389/150‑1 (MQ=255)
                                                                                cccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCATTTCAGCGGGACGCTGaaacgggaa                                                                   <  1:803963/150‑7 (MQ=37)
                                                                                 ccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCgg                                                                  <  1:113897/150‑1 (MQ=255)
                                                                                     ccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTa                                                              <  2:510433/150‑1 (MQ=255)
                                                                                       ccctGGTGTTCTTAGTAAGCCTTGAATCTAATCACTAAGAGTATCACCAGTATGATGACGGGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCTGCTTAcc                                                            <  1:1642580/150‑1 (MQ=255)
                                                                                         ctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcctc                                                          <  1:1637349/150‑1 (MQ=255)
                                                                                          tGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcctcc                                                         <  2:1556325/150‑1 (MQ=255)
                                                                                            gtgtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCtt                                                       <  2:54429/150‑1 (MQ=255)
                                                                                              gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                     >  2:658193/1‑150 (MQ=255)
                                                                                              gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                     >  1:707618/1‑150 (MQ=255)
                                                                                              gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                     >  2:1509676/1‑150 (MQ=255)
                                                                                                tCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGc                                                   >  1:1473086/1‑150 (MQ=255)
                                                                                                 cTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCggc                                                  <  1:251666/150‑3 (MQ=255)
                                                                                                  ttAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGccc                                                 <  1:1679496/150‑1 (MQ=255)
                                                                                                   tAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCTTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCt                                                >  1:1695899/1‑150 (MQ=255)
                                                                                                   tAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTACAGCGTCCCGCTGAAATCATCGGCTTACATCCTTTCGCCCt                                                >  1:1385995/1‑150 (MQ=255)
                                                                                                      tAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGaa                                             <  1:873899/150‑1 (MQ=255)
                                                                                                      tAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGaa                                             <  2:521030/150‑1 (MQ=255)
                                                                                                       aaGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAAt                                            >  1:1293307/1‑150 (MQ=255)
                                                                                                          ccTGGAAGATAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                         <  1:1501667/150‑1 (MQ=255)
                                                                                                           cTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAg                                        <  2:1343420/150‑1 (MQ=255)
                                                                                                            tGGAAGCTAATCACTAAGACTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGATGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGt                                       >  2:837421/1‑150 (MQ=255)
                                                                                                                aGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTACTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCCCTGAAAACATCGGATTACCTCCTTACCCCATGAAACCAGTCTaa                                   >  2:129296/1‑149 (MQ=25)
                                                                                                                 gCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATc                                  <  1:746104/150‑1 (MQ=255)
                                                                                                                  cTAATCACTACGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 <  2:121779/150‑1 (MQ=255)
                                                                                                                  cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 <  2:839489/150‑1 (MQ=255)
                                                                                                                  cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 >  1:490773/1‑150 (MQ=255)
                                                                                                                  cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 <  1:1407057/150‑1 (MQ=255)
                                                                                                                  cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                 <  1:782561/150‑1 (MQ=255)
                                                                                                                       cACTAAGCGTAGCACCAGTAGGATGACGTTCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTCCCTCCTTTCGCCCTGAAGGCAGTCTATCGCTAAc                            <  2:266688/150‑1 (MQ=21)
                                                                                                                        aCTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGATGAAATCTTCGGCTTACCTCATTTCGCCATGAATGCAGTctaacgctaacg                           >  1:212489/1‑150 (MQ=21)
                                                                                                                         cTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATAGGCTTACCTACTTTCGCCCTGAATGCAGTCTATCGCTAAcgc                          >  1:1380375/1‑150 (MQ=255)
                                                                                                                          tAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAAcgcg                         >  2:944900/1‑150 (MQ=255)
                                                                                                                           aaGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGt                        <  2:1334604/150‑1 (MQ=255)
                                                                                                                            agagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTg                       >  1:435465/1‑150 (MQ=255)
                                                                                                                                  tCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTACCGCTGAAATCATAGGCTTACCTCAGTTCGCCCTGATTGCAGTGTATCGCTAACGCGTGAATaat                 >  1:739076/1‑149 (MQ=37)
                                                                                                                                   cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                >  2:611563/1‑150 (MQ=255)
                                                                                                                                   cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                >  2:359562/1‑150 (MQ=255)
                                                                                                                                   cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCATGAATGCAGTATATCGCTAACGCGTGAATaaaa                >  2:411484/1‑150 (MQ=255)
                                                                                                                                     ccAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATa              >  2:354497/1‑150 (MQ=255)
                                                                                                                                      ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                >  1:597940/4‑147 (MQ=21)
                                                                                                                                      ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                <  2:597940/144‑1 (MQ=21)
                                                                                                                                      ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                <  2:1336172/144‑1 (MQ=21)
                                                                                                                                      ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                >  1:1336172/4‑147 (MQ=21)
                                                                                                                                      ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTACAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAg             >  1:973547/4‑150 (MQ=21)
                                                                                                                                         tATGATGACGTGCTTCATCATATCCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGGTTTCCCGTTTCAGCGTCCCGCTGAAATCTTCGTCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGttt          <  1:270727/150‑1 (MQ=255)
                                                                                                                                            gatgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCct       <  2:821346/150‑1 (MQ=255)
                                                                                                                                             atgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCctc      >  2:1472884/1‑150 (MQ=255)
                                                                                                                                              tgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCctct     <  1:1099591/150‑1 (MQ=255)
                                                                                                                                                 cGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCtgtg  >  1:348428/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTCGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTG  >  NC_000913/2153251‑2153545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: