Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,153,401 T→G 5.6% noncoding (93/143 nt)
K3T (AAG→ACG) 
sibA →
ibsA ←
sRNA antisense regulator of toxic IbsA protein
toxic membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,4010TG 5.6% 311.1 / 4.4 104noncoding (93/143 nt)
K3T (AAG→ACG) 
sibA
ibsA
sRNA antisense regulator of toxic IbsA protein
toxic membrane protein
Reads supporting (aligned to +/- strand):  ref base T (49/49);  new base G (3/3);  total (52/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

Only 100 of 106 total aligned reads displayed.

CGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTAT  >  NC_000913/2153253‑2153550
                                                                                                                                                    |                                                                                                                                                     
cGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTGTTAGTAAGCGTGGAAGATAATCAGTAAAATGATCTCCACTAAGCTGAAGTTCtt                                                                                                                                                      >  1:347898/1‑150 (MQ=255)
 gCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtc                                                                                                                                                     >  1:1302354/1‑150 (MQ=255)
  cAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtca                                                                                                                                                    >  1:821346/1‑150 (MQ=255)
  cAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtca                                                                                                                                                    <  1:1339949/150‑1 (MQ=255)
    aGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTtcatc                                                                                                                                                  <  2:802963/150‑1 (MQ=255)
         ggAGAGCCGTCGATTGAGACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACAAAGAGTATCACCAGTATGATGACGTGCTTCATCATAAc                                                                                                                                             <  1:280079/150‑1 (MQ=255)
         ggAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGAAGCACGTCATCATact                                                                                                                                             <  2:375133/150‑3 (MQ=255)
               ccGTCGTTTGACACTAATGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGGTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACAAGTATGATGACGTGCTTCATCATAACCCTTTc                                                                                                                                       <  2:900088/150‑1 (MQ=255)
               ccGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTc                                                                                                                                       >  2:301235/1‑150 (MQ=255)
                      ttGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCttatta                                                                                                                                <  2:970963/150‑1 (MQ=255)
                       tGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTaa                                                                                                                               <  2:1731871/150‑1 (MQ=255)
                        gACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGTTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGAAGTGCTTCATCATAACCCATTCATTATTaaa                                                                                                                              >  2:901321/1‑150 (MQ=255)
                        gACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTaaa                                                                                                                              <  2:1586908/150‑1 (MQ=255)
                        gACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATAATAACCCTTTCCTTATTaaa                                                                                                                              >  1:1475442/1‑150 (MQ=255)
                        gACACTAAGGGCGGAGTGACATAAATTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCGGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCCCCAGTATGATGACGTGCTTCATCATAACCATTTCCGTATTaaa                                                                                                                              >  2:270727/1‑150 (MQ=255)
                         acacTAAGGGCGGAGTGACATAATATCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTATTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGAGCTTCATCATAACCCTTACCTAATTaaaa                                                                                                                             >  2:1547844/1‑150 (MQ=39)
                          cacTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGATCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAg                                                                                                                            <  1:123798/150‑1 (MQ=255)
                            cTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGATAATCACTAAGAGTATAACCAGTATGATGAAGTGCTTCATCATAACCATTTCATTATTAAAAGcc                                                                                                                          >  2:1721029/1‑150 (MQ=255)
                                             tAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAgg                                                                                                         >  1:1304001/1‑150 (MQ=255)
                                              aaTTTCTGGAGTGAGGGTTAGCGAGAGGGTTCTCCCTCCCCCTTGTGTGCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGTCGTGCTTCTTCTTAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGc                                                                                                        <  1:1693323/150‑1 (MQ=255)
                                              aaTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCATGGAAGCTAATCACTAAGACTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGc                                                                                                        >  2:946545/1‑150 (MQ=255)
                                               aTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGAATCACCAGTATGATGACGTGCTTCATCATAACACTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGcg                                                                                                       >  2:1585234/1‑149 (MQ=255)
                                               aTTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTATTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCCTCATAAACCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCt                                                                                                       >  1:1013357/1‑150 (MQ=255)
                                                tttCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCtt                                                                                                      >  2:964805/1‑150 (MQ=255)
                                                   catgAGTGAGGGTTAGGGAGAGGTTTCCCCCTCAACATGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAATAGTATCACCAGTATGATGACGTGCTTCATCATAGCCCTTTCATTATTAAAAGCCCTCTTCTCAGGGAGAGGCTTTca                                                                                                   >  1:1014773/4‑149 (MQ=255)
                                                   cAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTcc                                                                                                   >  2:6142/1‑150 (MQ=255)
                                                    aGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTccc                                                                                                  <  1:1674373/150‑1 (MQ=255)
                                                      gAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGt                                                                                                <  2:827673/150‑1 (MQ=255)
                                                       aGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGtt                                                                                               >  2:1250316/1‑150 (MQ=255)
                                                       aGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGtt                                                                                               <  1:585578/150‑1 (MQ=255)
                                                        gTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGttt                                                                                              <  2:1073423/150‑1 (MQ=255)
                                                         ggAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTc                                                                                             <  2:55617/149‑1 (MQ=255)
                                                             ggTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCg                                                                                         >  1:436367/1‑150 (MQ=255)
                                                                tAGGGAGATGTTTACCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTcc                                                                                      <  2:1300429/150‑1 (MQ=255)
                                                                 aGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTccc                                                                                     <  2:1056641/150‑1 (MQ=255)
                                                                 aGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTccc                                                                                     >  1:1480777/1‑150 (MQ=255)
                                                                   ggAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTTATCACTAAGAGGATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCAAAGCTCCGGGAGCGCCTTTAAAGATTCAGCGTCCAGc                                                                                   >  2:774769/1‑150 (MQ=37)
                                                                   ggAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGc                                                                                   >  2:1812730/1‑150 (MQ=255)
                                                                     agagGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTg                                                                                 <  1:964805/150‑1 (MQ=255)
                                                                       agGTTTCCCCCTCCCACGGGGGTTCTTAGTAAGCCTGGAAGCTAATCACTAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTACCTTATTAAAATCCATCTTCTCAGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                              >  2:797451/1‑150 (MQ=37)
                                                                        ggTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                              >  2:665802/1‑150 (MQ=255)
                                                                        ggTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                              >  2:1019226/1‑150 (MQ=255)
                                                                         ggttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaac                                                                             <  2:225302/148‑2 (MQ=255)
                                                                         gTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaac                                                                             <  2:790455/150‑2 (MQ=255)
                                                                          tttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAata                                                                            >  2:408395/1‑149 (MQ=255)
                                                                           ttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                           <  1:702059/150‑4 (MQ=255)
                                                                             ccccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAatcatc                                                                         <  2:765389/150‑1 (MQ=255)
                                                                              cccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCATTTCAGCGGGACGCTGaaacgggaa                                                                        <  1:803963/150‑7 (MQ=37)
                                                                               ccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCgg                                                                       <  1:113897/150‑1 (MQ=255)
                                                                                   ccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTa                                                                   <  2:510433/150‑1 (MQ=255)
                                                                                     ccctGGTGTTCTTAGTAAGCCTTGAATCTAATCACTAAGAGTATCACCAGTATGATGACGGGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCTGCTTAcc                                                                 <  1:1642580/150‑1 (MQ=255)
                                                                                       ctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcctc                                                               <  1:1637349/150‑1 (MQ=255)
                                                                                        tGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcctcc                                                              <  2:1556325/150‑1 (MQ=255)
                                                                                          gtgtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCtt                                                            <  2:54429/150‑1 (MQ=255)
                                                                                            gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                          >  1:707618/1‑150 (MQ=255)
                                                                                            gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                          >  2:658193/1‑150 (MQ=255)
                                                                                            gtTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTc                                                          >  2:1509676/1‑150 (MQ=255)
                                                                                              tCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGc                                                        >  1:1473086/1‑150 (MQ=255)
                                                                                               cTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCggc                                                       <  1:251666/150‑3 (MQ=255)
                                                                                                ttAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGccc                                                      <  1:1679496/150‑1 (MQ=255)
                                                                                                 tAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCTTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCt                                                     >  1:1695899/1‑150 (MQ=255)
                                                                                                 tAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTACAGCGTCCCGCTGAAATCATCGGCTTACATCCTTTCGCCCt                                                     >  1:1385995/1‑150 (MQ=255)
                                                                                                    tAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGaa                                                  <  2:521030/150‑1 (MQ=255)
                                                                                                    tAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGaa                                                  <  1:873899/150‑1 (MQ=255)
                                                                                                     aaGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAAt                                                 >  1:1293307/1‑150 (MQ=255)
                                                                                                        ccTGGAAGATAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                              <  1:1501667/150‑1 (MQ=255)
                                                                                                         cTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAg                                             <  2:1343420/150‑1 (MQ=255)
                                                                                                          tGGAAGCTAATCACTAAGACTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGATGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGt                                            >  2:837421/1‑150 (MQ=255)
                                                                                                              aGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTACTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCCCTGAAAACATCGGATTACCTCCTTACCCCATGAAACCAGTCTaa                                        >  2:129296/1‑149 (MQ=25)
                                                                                                               gCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATc                                       <  1:746104/150‑1 (MQ=255)
                                                                                                                cTAATCACTACGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                      <  2:121779/150‑1 (MQ=255)
                                                                                                                cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                      <  1:1407057/150‑1 (MQ=255)
                                                                                                                cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                      <  1:782561/150‑1 (MQ=255)
                                                                                                                cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                      >  1:490773/1‑150 (MQ=255)
                                                                                                                cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCg                                      <  2:839489/150‑1 (MQ=255)
                                                                                                                 tAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGc                                     <  1:1812730/150‑1 (MQ=255)
                                                                                                                      aCTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGATGAAATCTTCGGCTTACCTCATTTCGCCATGAATGCAGTctaacgctaacg                                >  1:212489/1‑150 (MQ=21)
                                                                                                                       cTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATAGGCTTACCTACTTTCGCCCTGAATGCAGTCTATCGCTAAcgc                               >  1:1380375/1‑150 (MQ=255)
                                                                                                                        tAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCTTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAAcgcg                              <  2:428238/150‑1 (MQ=255)
                                                                                                                        tAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAAcgcg                              >  2:944900/1‑150 (MQ=255)
                                                                                                                         aaGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGt                             <  2:1334604/150‑1 (MQ=255)
                                                                                                                          agagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTg                            >  1:435465/1‑150 (MQ=255)
                                                                                                                                tCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTACCGCTGAAATCATAGGCTTACCTCAGTTCGCCCTGATTGCAGTGTATCGCTAACGCGTGAATaat                      >  1:739076/1‑149 (MQ=37)
                                                                                                                                 cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                     >  2:611563/1‑150 (MQ=255)
                                                                                                                                 cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                     >  2:359562/1‑150 (MQ=255)
                                                                                                                                 cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCATGAATGCAGTATATCGCTAACGCGTGAATaaaa                     >  2:411484/1‑150 (MQ=255)
                                                                                                                                   ccAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATa                   >  2:354497/1‑150 (MQ=255)
                                                                                                                                    ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                     >  1:1336172/4‑147 (MQ=21)
                                                                                                                                    ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                     <  2:597940/144‑1 (MQ=21)
                                                                                                                                    ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                     >  1:597940/4‑147 (MQ=21)
                                                                                                                                    ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                     <  2:1336172/144‑1 (MQ=21)
                                                                                                                                    ggttATGATGAAGCACGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTACAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAg                  >  1:973547/4‑150 (MQ=21)
                                                                                                                                       tATGATGACGTGCTTCATCATATCCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGGTTTCCCGTTTCAGCGTCCCGCTGAAATCTTCGTCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGttt               <  1:270727/150‑1 (MQ=255)
                                                                                                                                          gatgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCct            <  2:821346/150‑1 (MQ=255)
                                                                                                                                           atgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCctc           >  2:1472884/1‑150 (MQ=255)
                                                                                                                                            tgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCctct          <  1:1099591/150‑1 (MQ=255)
                                                                                                                                               cGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCtgtg       >  1:348428/1‑150 (MQ=255)
                                                                                                                                                  gCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGatt    >  1:1559032/1‑150 (MQ=255)
                                                                                                                                                   cTTCATCATAACCCTTTCCTTATTTAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGatta   <  1:618971/150‑1 (MQ=255)
                                                                                                                                                    ttCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTACCCGTTTCAGCGTCCCGCTGAAAGCATCGGCGTACCTCGTTTAGCCCTGAATGCAGTCTATAGCGAACGCGTGAATAAAATAGTATCCTCTGTGattat  >  2:819658/1‑150 (MQ=21)
                                                                                                                                                    |                                                                                                                                                     
CGCAAGAACGGAGAGCCGTCGATTGACACTAAGGGCGGAGTGACATAATTTCAGGAGTGAGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTAT  >  NC_000913/2153253‑2153550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: