Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,153,763 T→C 6.4% noncoding (120/136 nt) sibB → sRNA antisense regulator of toxic IbsB protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,7630TC 6.4% 370.0 / 10.8 125noncoding (120/136 nt)sibBsRNA antisense regulator of toxic IbsB protein
Reads supporting (aligned to +/- strand):  ref base T (63/52);  new base C (5/3);  total (69/56)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

Only 100 of 126 total aligned reads displayed.

CAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCAT  >  NC_000913/2153614‑2153908
                                                                                                                                                     |                                                                                                                                                 
cAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCtt                                                                                                                                                   >  1:1335133/1‑150 (MQ=255)
  aTCTTATAGTGAAGTCATACTTTCAGGAGTGCGGGGTCAGCGTGCTTTCCCCCTCCCTGCTAGTCTTAGTAAGCGGTGAATCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTcc                                                                                                                                                 <  2:272375/150‑1 (MQ=255)
    cTTATTGTGTATGGATTCTTTCATGAGTGTGGGTAGTGCGGGGTTTGCCCCGGCCTGGTTGTCTTAGTAAGCGGGTTAGGTTATTACTAAGAGCACCACGATGTTGTTTAGCTTGATGATGACCCTTTCCTTATTTATGGCCCCTTCCTc                                                                                                                                               <  2:60137/150‑1 (MQ=255)
    cTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTc                                                                                                                                               >  1:1149901/1‑150 (MQ=255)
     ttATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCg                                                                                                                                              <  2:503084/150‑1 (MQ=255)
       aTCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCggg                                                                                                                                            <  1:742633/150‑1 (MQ=255)
          gTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgg                                                                                                                                         >  1:1233895/1‑150 (MQ=255)
           tGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCACCGCCCTGGTAGTCTTAGTAAGCTGGTAATCTAATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAggg                                                                                                                                        <  2:530207/150‑1 (MQ=255)
            gAAGGCATTCTTTCATTAGGGAGGGTAGACCGCGGTTTCCCCCGCCCTTTTAGTCTTATTAAGCGGCGAAGGTTATGACTAAGTTCACCACTATGATGAGTAGCTTCATCATTACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgggg                                                                                                                                       <  2:739076/150‑1 (MQ=255)
            gAAGGCATACTTTCAGGAGGGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgggg                                                                                                                                       <  2:862603/150‑1 (MQ=255)
             aaGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGc                                                                                                                                      <  2:392161/150‑1 (MQ=255)
               ggCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTCCCGAGGAAGGGGcca                                                                                                                                    <  1:218117/150‑3 (MQ=255)
               ggCATACTTTCAGGAGGGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCtt                                                                                                                                    <  2:1479654/150‑1 (MQ=255)
                gCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTGATGCCTAAGCGCCCCACGATGCTGAGTAGCTTCATCATGGCCGTTTCCTGATTTATGGCCCCTTCCTCGGGAGGGGCttt                                                                                                                                   >  1:266111/1‑150 (MQ=255)
                 cATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTc                                                                                                                                  >  2:1604434/1‑150 (MQ=255)
                 cATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCGTAGTAAGCGGGGAAGCTTATGACTAAGAGCCCCACGATGCTGAGTAGCTTCAGCAGGACCCTTTCCTTATTTATGGCCACTTACTCGGGAGGGGCTTTc                                                                                                                                  >  2:1142607/1‑150 (MQ=255)
                  aTACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTcc                                                                                                                                 <  2:1814421/150‑1 (MQ=255)
                  aTACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTcc                                                                                                                                 <  1:613371/150‑1 (MQ=255)
                   tACTTTCAGGAGTGAGGGACGCGCGGGCTGTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTCCCGAGGAAGGGGccataaa                                                                                                                                <  1:774769/150‑7 (MQ=255)
                     cTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGt                                                                                                                              >  2:792352/1‑150 (MQ=255)
                      tttCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGtt                                                                                                                             >  1:1523220/1‑150 (MQ=255)
                        tCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTTGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGttta                                                                                                                           >  2:393919/1‑149 (MQ=255)
                        tCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTTGTAAGCGGGGAAGCTTATGACAAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTc                                                                                                                           >  2:130320/1‑150 (MQ=255)
                          cggAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAg                                                                                                                         <  1:1320707/149‑1 (MQ=255)
                          aGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTATCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAg                                                                                                                         <  1:520820/150‑1 (MQ=255)
                                  gggTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCAGGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGc                                                                                                                 >  2:724854/1‑150 (MQ=255)
                                   ggTATAGCGGGGTTACCTGAGCCCTGGTAGTCTTAGTAAGCGGGGATGCTTATGACTAAGAGCCCCACGATCATGCGTAGCTGCATCATGACCCTTTACGTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCTGCgggacgct                                                                                                                <  1:99709/150‑8 (MQ=25)
                                    gTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTg                                                                                                               >  2:106169/1‑150 (MQ=255)
                                     tAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAATAGCACCACGATGATGAGTAGATTCATGATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGAGTACCGCTGa                                                                                                              >  1:1023134/1‑150 (MQ=38)
                                     tAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGa                                                                                                              >  2:1655480/1‑150 (MQ=255)
                                     cacagCGGGGTTTCACCATCCCTGGTAGGCCTAGTAAGCTGTGAACCATATGAATACTAGCATCCCGATGACGAGTAGCTTCAGAATGACCATTTCCTTATATATGCCCCCTTCTTCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGa                                                                                                              <  2:1764173/147‑1 (MQ=16)
                                         gCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAAtc                                                                                                          >  2:950035/1‑150 (MQ=255)
                                          cGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGTTAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATCACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                                                         <  1:819658/150‑4 (MQ=37)
                                          cGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                                                         <  1:180880/150‑4 (MQ=255)
                                            gggTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAAtcgtc                                                                                                       <  1:1433248/150‑1 (MQ=255)
                                               tttCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGc                                                                                                    <  2:1073700/150‑1 (MQ=255)
                                                ttCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCt                                                                                                   >  1:1450082/1‑150 (MQ=255)
                                                 tCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                                                                                  <  2:866603/150‑1 (MQ=255)
                                                 tCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                                                                                  >  2:1564295/1‑150 (MQ=255)
                                                  cccccGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTa                                                                                                 >  1:785516/1‑150 (MQ=255)
                                                     ccGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcct                                                                                              >  2:789369/1‑150 (MQ=255)
                                                          cTGGTAGGCTTAGTCAGCGGGGAAGCGTATGTCTAAGAGCACCACGCTGCTGAGTAGCGTCATCATGCCCCTTTGCTTATTTCTGGCCCGTGCCGCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGTAATCGTCGGCTTACCTCCttt                                                                                         <  2:1624661/150‑1 (MQ=25)
                                                           tGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTc                                                                                        >  2:62893/1‑150 (MQ=255)
                                                            ggTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCg                                                                                       >  1:1494361/1‑150 (MQ=255)
                                                            ggTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCg                                                                                       <  1:883788/150‑1 (MQ=255)
                                                                gTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCAt                                                                                   >  1:522013/1‑150 (MQ=255)
                                                                 tCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATg                                                                                  <  1:1683136/150‑1 (MQ=255)
                                                                  cTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGc                                                                                 <  2:1827167/150‑1 (MQ=255)
                                                                   ttAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCa                                                                                <  2:1571200/150‑1 (MQ=255)
                                                                   ttAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCa                                                                                <  2:327799/150‑1 (MQ=255)
                                                                    tAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCaa                                                                               <  2:970585/150‑1 (MQ=255)
                                                                     aGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAg                                                                              >  1:1134339/1‑150 (MQ=255)
                                                                      gTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATATATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGc                                                                             >  1:699095/1‑150 (MQ=255)
                                                                        aaGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAg                                                                           >  1:49146/1‑150 (MQ=255)
                                                                          gCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCGTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGta                                                                         >  2:64474/1‑149 (MQ=255)
                                                                           cGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCt                                                                        >  1:1684603/1‑150 (MQ=255)
                                                                             gggAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAt                                                                      <  1:1157498/150‑1 (MQ=255)
                                                                                aaGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGc                                                                   <  1:776229/150‑1 (MQ=255)
                                                                                aaGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGc                                                                   >  1:1701881/1‑150 (MQ=255)
                                                                                 aGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGcg                                                                  >  1:699627/1‑149 (MQ=255)
                                                                                    ttATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc                                                               <  2:1382166/150‑1 (MQ=255)
                                                                                    ttATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc                                                               <  2:1374441/150‑1 (MQ=255)
                                                                                    ttATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCATTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCACGCTGAAATCGTCGGCTTACAGCCTGTCGCCATGCGATCAGTCTATCGCTAAc                                                               >  1:79425/1‑150 (MQ=25)
                                                                                     tATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcg                                                              >  2:1745424/1‑150 (MQ=255)
                                                                                     tATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcg                                                              >  1:188256/1‑150 (MQ=255)
                                                                                     tATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcg                                                              <  2:1253361/150‑1 (MQ=255)
                                                                                       tGACTAAGAGCACCACGATGATGAGTAGCTTCCTCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTACCGTTTCAGCGTCCCGCTGAAATCGTCGGCGTACCTCCTTTCGACAGGCAAGCAGTCATTCGCGAAcgcc                                                            >  1:1119256/1‑149 (MQ=25)
                                                                                               agCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATaaa                                                    >  2:813505/1‑150 (MQ=255)
                                                                                               agCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATaaa                                                    >  2:1570426/1‑150 (MQ=255)
                                                                                                        atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTcc                                           <  2:1585584/150‑1 (MQ=255)
                                                                                                        atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTcc                                           <  2:1270878/150‑1 (MQ=255)
                                                                                                        atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTcc                                           >  1:1432352/1‑150 (MQ=255)
                                                                                                          gatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCtg                                         <  2:1737189/150‑1 (MQ=255)
                                                                                                              aGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtt                                     <  2:1606703/150‑1 (MQ=255)
                                                                                                              aGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtt                                     >  1:945374/1‑150 (MQ=255)
                                                                                                              aGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtt                                     <  2:1335133/150‑1 (MQ=255)
                                                                                                                  gCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGttatta                                 >  1:413892/1‑150 (MQ=255)
                                                                                                                    ttCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACt                               >  1:396680/1‑150 (MQ=255)
                                                                                                                      catcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTgg                             <  1:128752/150‑1 (MQ=255)
                                                                                                                       ttcatTACCTTTTCTTTTTTTAAGGCCTCTTCGTCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGa                            <  1:129296/149‑1 (MQ=17)
                                                                                                                       atcatGCCCCTTTCCTTATTTATGGCACATGCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTCACTCCATTCGCCATGCAACCAGTCTATCCCTAACGCGTAGATAATATAGTATCCTGTGttattagagga                            >  2:6959/1‑145 (MQ=21)
                                                                                                                       atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCCGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGa                            >  1:1199753/1‑150 (MQ=255)
                                                                                                                         catGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATg                          <  1:62893/150‑1 (MQ=255)
                                                                                                                          atGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGc                         >  2:1790178/1‑150 (MQ=255)
                                                                                                                              cccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAGCGCTAACGCGGAGAGAAAATAGTTTCCTGTGTTATTTCTGGATGCGTGc                     >  2:334877/1‑150 (MQ=255)
                                                                                                                               ccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCt                    <  1:1304652/150‑1 (MQ=255)
                                                                                                                               ccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCt                    >  2:1235078/1‑150 (MQ=255)
                                                                                                                                 tttCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCg                  <  1:1647896/150‑1 (MQ=255)
                                                                                                                                    ccTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAAACGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAGCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGAAGCGTGCTCCCaa               >  1:909935/1‑150 (MQ=255)
                                                                                                                                     cTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCaaa              >  1:256964/1‑150 (MQ=255)
                                                                                                                                       tataaaaggCCTCTTCTCCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCTTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAAtg            <  2:212489/143‑1 (MQ=17)
                                                                                                                                         tttatGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAAtgtg          >  1:1369091/1‑150 (MQ=255)
                                                                                                                                          ggaaagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGc         >  1:101412/6‑150 (MQ=21)
                                                                                                                                           tatGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGcc        >  2:954177/1‑150 (MQ=255)
                                                                                                                                            aaagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGccc       >  1:1639052/4‑150 (MQ=21)
                                                                                                                                             tGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCg      >  2:1459922/1‑150 (MQ=255)
                                                                                                                                             aagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGGAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTTCTGGATGCGTGCTCGATAATGTGCCCg      >  1:605725/3‑150 (MQ=18)
                                                                                                                                             aagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCg      >  1:613502/3‑150 (MQ=255)
                                                                                                                                               gCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTc    >  2:446487/1‑150 (MQ=255)
                                                                                                                                               gCCCCTTCCTCGGGAGGGGCTTTCCCCATTCATCGTCCCGCTGAAATCGTCGGCTAACCTCCTTTCGCCATGAAAGCAGTCTATCGCTAACGCGTAGATAATATAGTTACCTGTGAGAATACTGCATGCGTCCACGCAAATGTGCCCgac    >  1:1739916/1‑148 (MQ=18)
                                                                                                                                                     |                                                                                                                                                 
CAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCAT  >  NC_000913/2153614‑2153908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: