Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,153,766 T→G 7.0% noncoding (123/136 nt) sibB → sRNA antisense regulator of toxic IbsB protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,7660TG 7.0% 353.1 / 12.1 125noncoding (123/136 nt)sibBsRNA antisense regulator of toxic IbsB protein
Reads supporting (aligned to +/- strand):  ref base T (65/50);  new base G (5/4);  total (70/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 125 total aligned reads displayed.

CTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCATTCAGAC  >  NC_000913/2153618‑2153914
                                                                                                                                                    |                                                                                                                                                    
cTTATTGTGTATGGATTCTTTCATGAGTGTGGGTAGTGCGGGGTTTGCCCCGGCCTGGTTGTCTTAGTAAGCGGGTTAGGTTATTACTAAGAGCACCACGATGTTGTTTAGCTTGATGATGACCCTTTCCTTATTTATGGCCCCTTCCTc                                                                                                                                                     <  2:60137/150‑1 (MQ=255)
cTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTc                                                                                                                                                     >  1:1149901/1‑150 (MQ=255)
 ttATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCg                                                                                                                                                    <  2:503084/150‑1 (MQ=255)
   aTCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCggg                                                                                                                                                  <  1:742633/150‑1 (MQ=255)
      gTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgg                                                                                                                                               >  1:1233895/1‑150 (MQ=255)
       tGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCACCGCCCTGGTAGTCTTAGTAAGCTGGTAATCTAATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAggg                                                                                                                                              <  2:530207/150‑1 (MQ=255)
        gAAGGCATTCTTTCATTAGGGAGGGTAGACCGCGGTTTCCCCCGCCCTTTTAGTCTTATTAAGCGGCGAAGGTTATGACTAAGTTCACCACTATGATGAGTAGCTTCATCATTACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgggg                                                                                                                                             <  2:739076/150‑1 (MQ=255)
        gAAGGCATACTTTCAGGAGGGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgggg                                                                                                                                             <  2:862603/150‑1 (MQ=255)
         aaGGCATACTTTCAGGTGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTCCCGAGGAAGGGGc                                                                                                                                            <  1:692690/150‑1 (MQ=255)
         aaGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGc                                                                                                                                            <  2:392161/150‑1 (MQ=255)
           ggCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTCCCGAGGAAGGGGcca                                                                                                                                          <  1:218117/150‑3 (MQ=255)
           ggCATACTTTCAGGAGGGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCtt                                                                                                                                          <  2:1479654/150‑1 (MQ=255)
            gCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTGATGCCTAAGCGCCCCACGATGCTGAGTAGCTTCATCATGGCCGTTTCCTGATTTATGGCCCCTTCCTCGGGAGGGGCttt                                                                                                                                         >  1:266111/1‑150 (MQ=255)
             cATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTc                                                                                                                                        >  2:1604434/1‑150 (MQ=255)
             cATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCGTAGTAAGCGGGGAAGCTTATGACTAAGAGCCCCACGATGCTGAGTAGCTTCAGCAGGACCCTTTCCTTATTTATGGCCACTTACTCGGGAGGGGCTTTc                                                                                                                                        >  2:1142607/1‑150 (MQ=255)
              aTACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTcc                                                                                                                                       <  2:1814421/150‑1 (MQ=255)
               tACTTTCAGGAGTGAGGGACGCGCGGGCTGTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTCCCGAGGAAGGGGccataaa                                                                                                                                      <  1:774769/150‑7 (MQ=255)
                 cTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGt                                                                                                                                    >  2:792352/1‑150 (MQ=255)
                  tttCAGGCGTGAGGGTTGAGCGGGGTTTCCCCCGCCCTGGTAGTCGTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGtt                                                                                                                                   <  1:206959/150‑1 (MQ=255)
                  tttCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGtt                                                                                                                                   >  1:1523220/1‑150 (MQ=255)
                   ttCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGttt                                                                                                                                  >  2:1304652/1‑150 (MQ=255)
                    tCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTTGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGttta                                                                                                                                 >  2:393919/1‑149 (MQ=255)
                    tCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTTGTAAGCGGGGAAGCTTATGACAAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTc                                                                                                                                 >  2:130320/1‑150 (MQ=255)
                      cggAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAg                                                                                                                               <  1:1320707/149‑1 (MQ=255)
                      aGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTATCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAg                                                                                                                               <  1:520820/150‑1 (MQ=255)
                              gggTAGCGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCGTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGc                                                                                                                       >  1:1443179/1‑150 (MQ=255)
                              gggTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCAGGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGc                                                                                                                       >  2:724854/1‑150 (MQ=255)
                               ggTATAGCGGGGTTACCTGAGCCCTGGTAGTCTTAGTAAGCGGGGATGCTTATGACTAAGAGCCCCACGATCATGCGTAGCTGCATCATGACCCTTTACGTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCTGCgggacgct                                                                                                                      <  1:99709/150‑8 (MQ=25)
                                gTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTg                                                                                                                     >  2:106169/1‑150 (MQ=255)
                                 tAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAATAGCACCACGATGATGAGTAGATTCATGATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGAGTACCGCTGa                                                                                                                    >  1:1023134/1‑150 (MQ=38)
                                 tAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGa                                                                                                                    >  2:1655480/1‑150 (MQ=255)
                                 cacagCGGGGTTTCACCATCCCTGGTAGGCCTAGTAAGCTGTGAACCATATGAATACTAGCATCCCGATGACGAGTAGCTTCAGAATGACCATTTCCTTATATATGCCCCCTTCTTCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGa                                                                                                                    <  2:1764173/147‑1 (MQ=16)
                                     gCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAAtc                                                                                                                >  2:950035/1‑150 (MQ=255)
                                      cGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGTTAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATCACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                                                               <  1:819658/150‑4 (MQ=37)
                                      cGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                                                               <  1:180880/150‑4 (MQ=255)
                                        gggTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAAtcgtc                                                                                                             <  1:1433248/150‑1 (MQ=255)
                                             tCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                                                                                        >  2:1572790/1‑150 (MQ=255)
                                             tCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                                                                                        >  2:1564295/1‑150 (MQ=255)
                                                 ccGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcct                                                                                                    >  2:789369/1‑150 (MQ=255)
                                                   gCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcctcc                                                                                                  <  1:1731410/150‑1 (MQ=255)
                                                    cccTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGTCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCCGCGTCCCGCTGAAATCGTCGGCTTAcctcct                                                                                                 >  2:1764835/1‑150 (MQ=255)
                                                      cTGGTAGGCTTAGTCAGCGGGGAAGCGTATGTCTAAGAGCACCACGCTGCTGAGTAGCGTCATCATGCCCCTTTGCTTATTTCTGGCCCGTGCCGCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGTAATCGTCGGCTTACCTCCttt                                                                                               <  2:1624661/150‑1 (MQ=25)
                                                       tGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTc                                                                                              >  2:62893/1‑150 (MQ=255)
                                                        ggTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCg                                                                                             <  1:883788/150‑1 (MQ=255)
                                                        ggTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCg                                                                                             >  1:1494361/1‑150 (MQ=255)
                                                            gTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCAt                                                                                         >  1:522013/1‑150 (MQ=255)
                                                             tCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATg                                                                                        <  1:1683136/150‑1 (MQ=255)
                                                               ttAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCa                                                                                      <  2:327799/150‑1 (MQ=255)
                                                               ttAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCa                                                                                      <  2:1571200/150‑1 (MQ=255)
                                                                tAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCaa                                                                                     <  2:970585/150‑1 (MQ=255)
                                                                 aGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAg                                                                                    >  1:1134339/1‑150 (MQ=255)
                                                                  gTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATATATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGc                                                                                   >  1:699095/1‑150 (MQ=255)
                                                                      gCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCGTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGta                                                                               >  2:64474/1‑149 (MQ=255)
                                                                      gCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTc                                                                               <  2:1478737/150‑1 (MQ=255)
                                                                       cGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCt                                                                              >  1:1684603/1‑150 (MQ=255)
                                                                         gggAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAt                                                                            <  1:1157498/150‑1 (MQ=255)
                                                                          ggAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATc                                                                           <  1:775813/150‑1 (MQ=255)
                                                                            aaGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGc                                                                         >  1:1701881/1‑150 (MQ=255)
                                                                            aaGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGc                                                                         <  1:776229/150‑1 (MQ=255)
                                                                             aGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCt                                                                        >  2:18364/1‑150 (MQ=255)
                                                                                ttATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc                                                                     <  2:1374441/150‑1 (MQ=255)
                                                                                ttATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCATTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCACGCTGAAATCGTCGGCTTACAGCCTGTCGCCATGCGATCAGTCTATCGCTAAc                                                                     >  1:79425/1‑150 (MQ=25)
                                                                                 tATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcg                                                                    <  2:1253361/150‑1 (MQ=255)
                                                                                 tATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcg                                                                    >  1:188256/1‑150 (MQ=255)
                                                                                   tGACTAAGAGCACCACGATGATGAGTAGCTTCCTCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTACCGTTTCAGCGTCCCGCTGAAATCGTCGGCGTACCTCCTTTCGACAGGCAAGCAGTCATTCGCGAAcgcc                                                                  >  1:1119256/1‑149 (MQ=25)
                                                                                     aCTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTa                                                                >  1:588558/1‑150 (MQ=255)
                                                                                           agCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATaaa                                                          >  2:813505/1‑150 (MQ=255)
                                                                                                    atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTcc                                                 >  1:1432352/1‑150 (MQ=255)
                                                                                                    atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTcc                                                 <  2:1585584/150‑1 (MQ=255)
                                                                                                      gatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCtg                                               >  1:411793/1‑150 (MQ=255)
                                                                                                      gatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCtg                                               <  2:1737189/150‑1 (MQ=255)
                                                                                                          aGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtt                                           <  2:1335133/150‑1 (MQ=255)
                                                                                                          aGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtt                                           >  1:945374/1‑150 (MQ=255)
                                                                                                           gTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtta                                          <  2:1149901/150‑1 (MQ=255)
                                                                                                              gCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGttatta                                       >  1:413892/1‑150 (MQ=255)
                                                                                                              gCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATAGCTAACGCGTAGATAATATAGTTTCCTGTGttatta                                       >  2:1625926/1‑150 (MQ=255)
                                                                                                                ttCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACt                                     >  1:396680/1‑150 (MQ=255)
                                                                                                                  catcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTgg                                   <  1:128752/150‑1 (MQ=255)
                                                                                                                   ttcatTACCTTTTCTTTTTTTAAGGCCTCTTCGTCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGa                                  <  1:129296/149‑1 (MQ=17)
                                                                                                                   atcatGCCCCTTTCCTTATTTATGGCACATGCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTCACTCCATTCGCCATGCAACCAGTCTATCCCTAACGCGTAGATAATATAGTATCCTGTGttattagagga                                  >  2:6959/1‑145 (MQ=21)
                                                                                                                   atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGa                                  >  1:96901/1‑150 (MQ=255)
                                                                                                                   atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCCGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGa                                  >  1:1199753/1‑150 (MQ=255)
                                                                                                                     catGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATg                                <  1:62893/150‑1 (MQ=255)
                                                                                                                      atGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGc                               >  2:1790178/1‑150 (MQ=255)
                                                                                                                        gACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTACTGTGTTATTACTGGATGCGt                             >  2:884178/1‑150 (MQ=255)
                                                                                                                          cccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAGCGCTAACGCGGAGAGAAAATAGTTTCCTGTGTTATTTCTGGATGCGTGc                           >  2:334877/1‑150 (MQ=255)
                                                                                                                           ccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCt                          <  1:1304652/150‑1 (MQ=255)
                                                                                                                           ccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCt                          >  2:1235078/1‑150 (MQ=255)
                                                                                                                             tttCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTACTTTCGCCATGCAAGCAGTCTATCGCTACCGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCg                        >  1:878916/1‑150 (MQ=255)
                                                                                                                                   tataaaaggCCTCTTCTCCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCTTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAAtg                  <  2:212489/143‑1 (MQ=17)
                                                                                                                                     tttatGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAAtgtg                >  1:1369091/1‑150 (MQ=255)
                                                                                                                                      ggaaagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGc               >  1:101412/6‑150 (MQ=21)
                                                                                                                                       tatGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGcc              >  2:954177/1‑150 (MQ=255)
                                                                                                                                        aaagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGccc             >  1:1639052/4‑150 (MQ=21)
                                                                                                                                         tGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCg            >  2:1459922/1‑150 (MQ=255)
                                                                                                                                         aagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGGAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTTCTGGATGCGTGCTCGATAATGTGCCCg            >  1:605725/3‑150 (MQ=18)
                                                                                                                                         aagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCg            >  1:613502/3‑150 (MQ=255)
                                                                                                                                           gCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTc          >  2:446487/1‑150 (MQ=255)
                                                                                                                                             cccTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCAt        <  1:1646606/150‑1 (MQ=255)
                                                                                                                                                   cTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCATTCAGAc  >  2:789945/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCATTCAGAC  >  NC_000913/2153618‑2153914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: