Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,153,770 G→A 6.6% noncoding (127/136 nt) sibB → sRNA antisense regulator of toxic IbsB protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,7700GA 6.6% 371.5 / 9.7 121noncoding (127/136 nt)sibBsRNA antisense regulator of toxic IbsB protein
Reads supporting (aligned to +/- strand):  ref base G (64/48);  new base A (5/3);  total (69/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 122 total aligned reads displayed.

ATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCATTCAGAC  >  NC_000913/2153621‑2153914
                                                                                                                                                     |                                                                                                                                                
   gTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgg                                                                                                                                               >  1:1233895/1‑150 (MQ=255)
    tGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCACCGCCCTGGTAGTCTTAGTAAGCTGGTAATCTAATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAggg                                                                                                                                              <  2:530207/150‑1 (MQ=255)
    tGAAGGAATACTTTAAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTGTGTAACCGGGGAAGCTTTTGACTAAGAGCACCACGATGTTGAGTAGCTTCATCATGGAGCTTTCCGTATTTAGTGCCCCTTCCTCGGGAggg                                                                                                                                              >  1:125080/1‑150 (MQ=255)
     gAATGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCGCGCCCTGGTAGTCTTAGTAAGCGGTGAATCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGTAgggg                                                                                                                                             <  1:1325198/150‑1 (MQ=255)
     gAAGGCATTCTTTCATTAGGGAGGGTAGACCGCGGTTTCCCCCGCCCTTTTAGTCTTATTAAGCGGCGAAGGTTATGACTAAGTTCACCACTATGATGAGTAGCTTCATCATTACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgggg                                                                                                                                             <  2:739076/150‑1 (MQ=255)
     gAAGGCATACTTTCAGGAGGGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAgggg                                                                                                                                             <  2:862603/150‑1 (MQ=255)
      aaGGCATACTTTCAGGTGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTCCCGAGGAAGGGGc                                                                                                                                            <  1:692690/150‑1 (MQ=255)
        ggCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTCCCGAGGAAGGGGcca                                                                                                                                          <  1:218117/150‑3 (MQ=255)
        ggCATACTTTCAGGAGGGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCtt                                                                                                                                          <  2:1479654/150‑1 (MQ=255)
         gCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTGATGCCTAAGCGCCCCACGATGCTGAGTAGCTTCATCATGGCCGTTTCCTGATTTATGGCCCCTTCCTCGGGAGGGGCttt                                                                                                                                         >  1:266111/1‑150 (MQ=255)
          cATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTc                                                                                                                                        >  2:1604434/1‑150 (MQ=255)
          cATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCGTAGTAAGCGGGGAAGCTTATGACTAAGAGCCCCACGATGCTGAGTAGCTTCAGCAGGACCCTTTCCTTATTTATGGCCACTTACTCGGGAGGGGCTTTc                                                                                                                                        >  2:1142607/1‑150 (MQ=255)
           aTACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTcc                                                                                                                                       <  2:1814421/150‑1 (MQ=255)
           aTACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTcc                                                                                                                                       <  1:613371/150‑1 (MQ=255)
            tACTTTCAGGAGTGAGGGACGCGCGGGCTGTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTCCCGAGGAAGGGGccataaa                                                                                                                                      <  1:774769/150‑7 (MQ=255)
              cTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGt                                                                                                                                    >  2:792352/1‑150 (MQ=255)
               tttCAGGCGTGAGGGTTGAGCGGGGTTTCCCCCGCCCTGGTAGTCGTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGtt                                                                                                                                   <  1:206959/150‑1 (MQ=255)
               tttCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGtt                                                                                                                                   >  1:1523220/1‑150 (MQ=255)
                ttCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGttt                                                                                                                                  >  2:1304652/1‑150 (MQ=255)
                 tCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTTGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGttta                                                                                                                                 >  2:393919/1‑149 (MQ=255)
                 tCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTTGTAAGCGGGGAAGCTTATGACAAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTc                                                                                                                                 >  2:130320/1‑150 (MQ=255)
                   cggAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAg                                                                                                                               <  1:1320707/149‑1 (MQ=255)
                   aGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTATCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAg                                                                                                                               <  1:520820/150‑1 (MQ=255)
                           gggTAGCGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCGTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGc                                                                                                                       >  1:1443179/1‑150 (MQ=255)
                           gggTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCAGGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGc                                                                                                                       >  2:724854/1‑150 (MQ=255)
                            ggTATAGCGGGGTTACCTGAGCCCTGGTAGTCTTAGTAAGCGGGGATGCTTATGACTAAGAGCCCCACGATCATGCGTAGCTGCATCATGACCCTTTACGTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCTGCgggacgct                                                                                                                      <  1:99709/150‑8 (MQ=25)
                             gTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTg                                                                                                                     >  2:106169/1‑150 (MQ=255)
                              tAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAATAGCACCACGATGATGAGTAGATTCATGATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGAGTACCGCTGa                                                                                                                    >  1:1023134/1‑150 (MQ=38)
                              tAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGa                                                                                                                    >  2:1655480/1‑150 (MQ=255)
                              cacagCGGGGTTTCACCATCCCTGGTAGGCCTAGTAAGCTGTGAACCATATGAATACTAGCATCCCGATGACGAGTAGCTTCAGAATGACCATTTCCTTATATATGCCCCCTTCTTCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGa                                                                                                                    <  2:1764173/147‑1 (MQ=16)
                                  gCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAAtc                                                                                                                >  2:950035/1‑150 (MQ=255)
                                   cGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                                                               <  1:180880/150‑4 (MQ=255)
                                        tttCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGc                                                                                                          <  2:1073700/150‑1 (MQ=255)
                                         ttCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCt                                                                                                         >  1:1450082/1‑150 (MQ=255)
                                          tCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                                                                                        >  2:1572790/1‑150 (MQ=255)
                                           cccccGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTa                                                                                                       >  1:785516/1‑150 (MQ=255)
                                                 cccTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGTCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCCGCGTCCCGCTGAAATCGTCGGCTTAcctcct                                                                                                 >  2:1764835/1‑150 (MQ=255)
                                                   cTGGTAGGCTTAGTCAGCGGGGAAGCGTATGTCTAAGAGCACCACGCTGCTGAGTAGCGTCATCATGCCCCTTTGCTTATTTCTGGCCCGTGCCGCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGTAATCGTCGGCTTACCTCCttt                                                                                               <  2:1624661/150‑1 (MQ=25)
                                                    tGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTc                                                                                              >  2:62893/1‑150 (MQ=255)
                                                     ggTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCg                                                                                             <  1:883788/150‑1 (MQ=255)
                                                     ggTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCg                                                                                             >  1:1494361/1‑150 (MQ=255)
                                                         gTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCAt                                                                                         >  1:522013/1‑150 (MQ=255)
                                                          tCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATg                                                                                        <  1:1683136/150‑1 (MQ=255)
                                                           cTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGc                                                                                       <  2:1827167/150‑1 (MQ=255)
                                                            ttAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCa                                                                                      <  2:1571200/150‑1 (MQ=255)
                                                            ttAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCa                                                                                      <  2:327799/150‑1 (MQ=255)
                                                             tAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCaa                                                                                     <  2:970585/150‑1 (MQ=255)
                                                             tAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCaa                                                                                     <  2:52360/150‑1 (MQ=255)
                                                              aGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAg                                                                                    >  1:1134339/1‑150 (MQ=255)
                                                               gTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATATATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGc                                                                                   >  1:699095/1‑150 (MQ=255)
                                                                   gCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTc                                                                               <  2:1478737/150‑1 (MQ=255)
                                                                    cGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCt                                                                              >  1:1684603/1‑150 (MQ=255)
                                                                       ggAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATc                                                                           <  1:775813/150‑1 (MQ=255)
                                                                         aaGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGc                                                                         <  1:776229/150‑1 (MQ=255)
                                                                         aaGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGc                                                                         >  1:1701881/1‑150 (MQ=255)
                                                                          aGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGcg                                                                        >  1:699627/1‑149 (MQ=255)
                                                                             ttATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc                                                                     <  2:1382166/150‑1 (MQ=255)
                                                                             ttATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCATTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCACGCTGAAATCGTCGGCTTACAGCCTGTCGCCATGCGATCAGTCTATCGCTAAc                                                                     >  1:79425/1‑150 (MQ=25)
                                                                              tATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcg                                                                    >  1:188256/1‑150 (MQ=255)
                                                                              tATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcg                                                                    <  2:1253361/150‑1 (MQ=255)
                                                                                tGACTAAGAGCACCACGATGATGAGTAGCTTCCTCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTACCGTTTCAGCGTCCCGCTGAAATCGTCGGCGTACCTCCTTTCGACAGGCAAGCAGTCATTCGCGAAcgcc                                                                  >  1:1119256/1‑149 (MQ=25)
                                                                                  aCTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTa                                                                >  1:588558/1‑150 (MQ=255)
                                                                                        agCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATaaa                                                          >  2:813505/1‑150 (MQ=255)
                                                                                        agCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATaaa                                                          >  2:1570426/1‑150 (MQ=255)
                                                                                                 atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTcc                                                 <  2:1585584/150‑1 (MQ=255)
                                                                                                 atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTcc                                                 <  2:1270878/150‑1 (MQ=255)
                                                                                                 atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTcc                                                 >  1:1432352/1‑150 (MQ=255)
                                                                                                   gatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCtg                                               >  1:411793/1‑150 (MQ=255)
                                                                                                   gatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCtg                                               <  2:1737189/150‑1 (MQ=255)
                                                                                                       aGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtt                                           <  2:1335133/150‑1 (MQ=255)
                                                                                                       aGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtt                                           >  1:945374/1‑150 (MQ=255)
                                                                                                        gTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGtta                                          <  2:1149901/150‑1 (MQ=255)
                                                                                                           gCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGttatta                                       >  1:413892/1‑150 (MQ=255)
                                                                                                           gCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATAGCTAACGCGTAGATAATATAGTTTCCTGTGttatta                                       >  2:1625926/1‑150 (MQ=255)
                                                                                                             ttCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACt                                     >  1:396680/1‑150 (MQ=255)
                                                                                                               catcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTgg                                   <  1:128752/150‑1 (MQ=255)
                                                                                                                ttcatTACCTTTTCTTTTTTTAAGGCCTCTTCGTCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGa                                  <  1:129296/149‑1 (MQ=17)
                                                                                                                atcatGCCCCTTTCCTTATTTATGGCACATGCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTCACTCCATTCGCCATGCAACCAGTCTATCCCTAACGCGTAGATAATATAGTATCCTGTGttattagagga                                  >  2:6959/1‑145 (MQ=21)
                                                                                                                atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCCGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGa                                  >  1:1199753/1‑150 (MQ=255)
                                                                                                                  catGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATg                                <  1:62893/150‑1 (MQ=255)
                                                                                                                  catGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATg                                >  2:360276/1‑150 (MQ=255)
                                                                                                                   atGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGc                               >  2:1790178/1‑150 (MQ=255)
                                                                                                                     gACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTACTGTGTTATTACTGGATGCGt                             >  2:884178/1‑150 (MQ=255)
                                                                                                                       cccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAGCGCTAACGCGGAGAGAAAATAGTTTCCTGTGTTATTTCTGGATGCGTGc                           >  2:334877/1‑150 (MQ=255)
                                                                                                                          tttCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCg                        <  1:1647896/150‑1 (MQ=255)
                                                                                                                          tttCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTACTTTCGCCATGCAAGCAGTCTATCGCTACCGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCg                        >  1:878916/1‑150 (MQ=255)
                                                                                                                             ccTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAAACGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAGCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGAAGCGTGCTCCCaa                     >  1:909935/1‑150 (MQ=255)
                                                                                                                              cTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTTTTACTGGATGCGGGCTCGCaat                    >  2:66783/1‑149 (MQ=255)
                                                                                                                                tatttatGGCCCCTTCCTCGGGAGGGGCTGTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTAACCTCCTTTCGCCCTGCACGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAAtg                  <  1:1142607/150‑1 (MQ=255)
                                                                                                                                tataaaaggCCTCTTCTCCGGGAGTGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCTTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAAtg                  <  2:212489/143‑1 (MQ=17)
                                                                                                                                  tttatGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAAtgtg                >  1:1369091/1‑150 (MQ=255)
                                                                                                                                   ggaaagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGc               >  2:789935/6‑150 (MQ=21)
                                                                                                                                   ggaaagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGc               >  1:101412/6‑150 (MQ=21)
                                                                                                                                    tatGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGcc              >  2:954177/1‑150 (MQ=255)
                                                                                                                                     aaagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGccc             >  1:1639052/4‑150 (MQ=21)
                                                                                                                                      tGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCg            >  2:1459922/1‑150 (MQ=255)
                                                                                                                                      aagCCCCTCCCGAGGAAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGGAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTTCTGGATGCGTGCTCGATAATGTGCCCg            >  1:605725/3‑150 (MQ=18)
                                                                                                                                        gCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTc          >  2:446487/1‑150 (MQ=255)
                                                                                                                                        gCCCCTTCCTCGGGAGGGGCTTTCCCCATTCATCGTCCCGCTGAAATCGTCGGCTAACCTCCTTTCGCCATGAAAGCAGTCTATCGCTAACGCGTAGATAATATAGTTACCTGTGAGAATACTGCATGCGTCCACGCAAATGTGCCCgac          >  1:1739916/1‑148 (MQ=18)
                                                                                                                                                cTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCATTCAGAc  >  2:789945/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
ATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCATTCAGAC  >  NC_000913/2153621‑2153914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: