Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,151 C→T 5.5% pseudogene (212/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,1510CT 5.5% 399.3 / 7.6 129pseudogene (212/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (65/55);  new base T (5/2);  total (71/58)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 130 total aligned reads displayed.

TGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATT  >  NC_000913/2173002‑2173298
                                                                                                                                                     |                                                                                                                                                   
  cACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaa                                                                                                                                                   <  1:1713048/150‑1 (MQ=255)
  cACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaa                                                                                                                                                   <  1:1719959/150‑1 (MQ=255)
   aCCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAAt                                                                                                                                                  <  1:1454673/150‑1 (MQ=255)
    ccGAAAATAATGAAACCGTGAATAATTAGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCTGCTTATAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATa                                                                                                                                                 <  1:234282/150‑1 (MQ=255)
      gATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGt                                                                                                                                               >  1:1238460/1‑150 (MQ=255)
        taataaTGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCGGCCAGTTGTGTGTGCAAACCAAGAGgtt                                                                                                                                             >  2:208123/1‑150 (MQ=255)
        taataaTGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGttt                                                                                                                                             <  2:130386/150‑1 (MQ=255)
         aataatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTg                                                                                                                                            <  2:1076550/150‑1 (MQ=255)
            aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGTGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGGTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTCTGCAAACCAATATTTTGCGt                                                                                                                                         >  2:37729/1‑150 (MQ=255)
            aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcac                                                                                                                                         <  1:766767/150‑3 (MQ=255)
            aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGt                                                                                                                                         >  1:1307046/1‑150 (MQ=255)
              tGAAACCGGGAAAATTTTGCGCTGAGAATACCTGAATAAGTAACCATGTTATGTGAGTACCGCCCTGATCCATTGAAGCCACCATACCCGCGGCTTTTAACGCCCCAGCATTATCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcacac                                                                                                                                       <  1:1096105/150‑5 (MQ=255)
               gAAACCGTGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTTATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                      <  2:283408/150‑1 (MQ=255)
               gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCTAACCAATAGTTTGCGTcgc                                                                                                                                      >  2:171080/1‑150 (MQ=255)
               gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                      <  1:200723/150‑1 (MQ=255)
               gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAAACAGTAACCAGGTAATGGGAGAACCTCCCTGATCAATTGAAGCCACCATACACCCGGCTTTTAACGCCCCAGCAATAGCCGCCAGTTGGCTGAGCAACCAAATAGTTTGAGTcgg                                                                                                                                      >  2:1344924/1‑149 (MQ=255)
                aaaCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgcg                                                                                                                                     <  2:1789528/150‑1 (MQ=255)
                  aCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAAACGCCCTGATCCATTGACGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGGGTGCAAACCAATAGGTTGCGTCGCGAt                                                                                                                                   <  2:937831/150‑1 (MQ=255)
                    cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                 <  2:749228/150‑1 (MQ=255)
                    cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                 >  1:1810317/1‑150 (MQ=255)
                    cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                 <  1:1638622/150‑1 (MQ=255)
                      ggAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCcac                                                                                                                               >  2:1500229/1‑150 (MQ=255)
                           atTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGt                                                                                                                          <  1:1126358/150‑1 (MQ=255)
                             tttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGa                                                                                                                        <  1:1466995/150‑1 (MQ=255)
                              ttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGac                                                                                                                       >  2:78492/1‑149 (MQ=255)
                                    ggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCGATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCAt                                                                                                                 >  1:992969/1‑150 (MQ=255)
                                       gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAat                                                                                                              <  1:825548/150‑1 (MQ=255)
                                       gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAat                                                                                                              >  2:219167/1‑150 (MQ=255)
                                        aaaaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATaacg                                                                                                             <  2:1655227/150‑3 (MQ=255)
                                         aaaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAACCCAATAGTTTGCGTCGCGATCCACAGGGTGCTGCTCATAatga                                                                                                            >  2:1740583/1‑150 (MQ=255)
                                          aaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCACACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgat                                                                                                           >  1:1614657/1‑150 (MQ=255)
                                          aaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgat                                                                                                           <  2:25156/150‑1 (MQ=255)
                                           aCCTGAATCAGTAACCATGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgatg                                                                                                          <  2:735219/150‑1 (MQ=255)
                                              tGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGaca                                                                                                       >  1:1805268/1‑150 (MQ=255)
                                               gAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGacac                                                                                                      >  2:522549/1‑150 (MQ=255)
                                                    aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATCCCCCCGGCTTTTACCGCCCCGGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGGGTCGCGATCCCCAGGGTGATGCTCTTATTGATGCCAcccatg                                                                                                 >  2:870433/1‑147 (MQ=255)
                                                    aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCgag                                                                                                 >  2:1494757/1‑150 (MQ=255)
                                                    aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCgag                                                                                                 <  1:764306/150‑1 (MQ=255)
                                                       aaCCAGGTAATGGGAGAACCGCCATGATACATTGAAGACACCATACCCCCGGCTTTTAACGCACCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGATCCACAGGGTGATGCTAATAATGATGACACCCGAGAtt                                                                                              >  2:1134543/1‑150 (MQ=255)
                                                         ccAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTaa                                                                                            <  1:1512925/150‑1 (MQ=255)
                                                         ccAGGTAATGGGAGAACCGCCCTGATCCATCTAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTaa                                                                                            >  2:1536081/1‑150 (MQ=255)
                                                           aGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAgg                                                                                          <  1:1593606/150‑1 (MQ=255)
                                                           aGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAgg                                                                                          <  1:1600595/150‑1 (MQ=255)
                                                           aGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAgg                                                                                          <  1:942924/150‑1 (MQ=255)
                                                               taTGGGTGATTCGCCATGATCCATTGAAGCCACCATACCCCGGGCTTTTAACTCCCCAGTATTAGCCGCCAGTTGTTTGTGCAAACCATTTGTTTGCGTCGCGATCCACAGTGTGTTGCTCTTAATTATGACACCCGAGTTTAAGGTGCg                                                                                      <  2:1540943/149‑1 (MQ=255)
                                                               aaTGGGAGAACCGCCCTGATCCAATGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTAGGGTGTGCAAAGCAATAGTTTGCGTCGCGATGCACAGGGTGATGCTCATAATGATGACACCCGAGATTAACGTGCg                                                                                      >  2:127933/1‑150 (MQ=255)
                                                                 tGGGAGAACCGCCCTGATCCATTGACGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGa                                                                                    >  1:108264/1‑150 (MQ=255)
                                                                   ggAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAAc                                                                                  <  2:1249674/150‑1 (MQ=255)
                                                                        aCCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCACTAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGAtt                                                                             <  2:913526/150‑1 (MQ=255)
                                                                          cGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTc                                                                           <  1:1589403/150‑1 (MQ=255)
                                                                           gCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTcc                                                                          >  1:1186076/1‑150 (MQ=255)
                                                                             ccTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCTCCAGTGGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCAc                                                                        <  1:846259/150‑1 (MQ=255)
                                                                              cTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGCTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACg                                                                       >  1:1414549/1‑150 (MQ=255)
                                                                                  tCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGCTCCACCGGGTGTTGCTCATAATGATGACACCCGAGATTATGGAGCGGAAAAGATTTACACGATGc                                                                   >  1:901512/1‑150 (MQ=255)
                                                                                  tCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGc                                                                   >  2:1032406/1‑150 (MQ=255)
                                                                                   ccATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCa                                                                  >  1:1695719/1‑150 (MQ=255)
                                                                                    cATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAAAAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGCCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCAc                                                                 >  1:742221/1‑150 (MQ=255)
                                                                                       tGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGc                                                              >  2:595666/1‑150 (MQ=255)
                                                                                       tGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGc                                                              >  2:136935/1‑150 (MQ=255)
                                                                                        gacgCCCCCATACCCCCGGCTTTTAACGCCCCAGCACTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGCGCGGAACAGATTTCCACGATGCACGGCg                                                             <  2:959756/147‑1 (MQ=255)
                                                                                        gAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCATACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCg                                                             >  1:753218/1‑150 (MQ=255)
                                                                                             caccaTACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGc                                                        <  1:980534/150‑1 (MQ=255)
                                                                                                  tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                   <  2:479977/150‑1 (MQ=255)
                                                                                                  tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                   >  1:603062/1‑150 (MQ=255)
                                                                                                  aaCCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                   <  1:843269/149‑1 (MQ=255)
                                                                                                       ccGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACg                                              >  1:1600713/1‑150 (MQ=255)
                                                                                                         ggCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaa                                            <  2:1695719/150‑1 (MQ=255)
                                                                                                            ttttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCCCgaagca                                         >  2:166797/1‑148 (MQ=255)
                                                                                                              ttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGc                                       >  2:511059/1‑150 (MQ=255)
                                                                                                               tAACGCCCCATAATTAGCCGCCAGTTGTGTGTGCAAACGAATAGTTTGCGTCGCGATCGACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGcc                                      <  2:866529/150‑1 (MQ=255)
                                                                                                               tAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCCCGAAGAAGcc                                      >  1:703863/1‑150 (MQ=255)
                                                                                                                aaCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCg                                     >  2:1312916/1‑150 (MQ=255)
                                                                                                                aaCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTTAGGTGCGGAACAGATTTCCACGATGCACGGCGACCTCCATCGCCAAGAAGAAGCCg                                     >  2:1109521/1‑150 (MQ=255)
                                                                                                                aaCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTACCACGATGCACGGCGACCGCCATCGCCACCAAGAAGCCg                                     >  1:1295673/1‑150 (MQ=255)
                                                                                                                aaCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGAGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGACCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCg                                     >  1:118342/1‑150 (MQ=255)
                                                                                                                 aCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCATACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCAAAATTATGAAACACGAGATTAAGGTTCTGAACAGATTTCCCCGAGGCACGGCGACCGCCATCGCCACGAAGACGCCGa                                    >  2:879058/1‑150 (MQ=255)
                                                                                                                  cGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAt                                   >  1:497595/1‑150 (MQ=255)
                                                                                                                  cGCCCCAGCATTAGCCGCCAGCTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCAGAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAt                                   >  1:1585421/1‑150 (MQ=255)
                                                                                                                   gCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGCTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAtg                                  >  1:550504/1‑150 (MQ=255)
                                                                                                                               gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGCTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                      <  1:389369/150‑1 (MQ=255)
                                                                                                                               gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                      >  2:1343276/1‑150 (MQ=255)
                                                                                                                               gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                      <  2:1251004/150‑1 (MQ=255)
                                                                                                                               gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                      >  2:610797/1‑150 (MQ=255)
                                                                                                                                ccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGc                     <  1:1433970/150‑1 (MQ=255)
                                                                                                                                 cgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGcc                    >  1:738089/1‑150 (MQ=255)
                                                                                                                                      gttgggttggCAAACCAATAGTTTGCGGCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGCTTAAGGTGCGGAACAGATTTCCACGATGCATGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACg               <  1:1121341/150‑1 (MQ=255)
                                                                                                                                      gTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAcc               >  1:311454/1‑149 (MQ=255)
                                                                                                                                        tGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAATAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgc             >  1:1512329/1‑150 (MQ=255)
                                                                                                                                          ggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcag           >  1:668096/1‑150 (MQ=255)
                                                                                                                                          ggTGTGCAAACCAATAGTTTGAGTCGCGATCAACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAAAAGATTTCCACGATGCACGGCGACCGCCATCGACACGAAGAAGCCGATGGTGGCAAGATAGCCAAACGgcag           >  1:455195/1‑150 (MQ=255)
                                                                                                                                           cgacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagc          >  1:1484842/5‑150 (MQ=255)
                                                                                                                                           cgacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagc          >  2:763581/5‑150 (MQ=255)
                                                                                                                                            gacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTCATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagcc         >  1:351055/4‑149 (MQ=255)
                                                                                                                                              ttcAAACCAATAGGTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGCCACCCGAGATTTAGGTGCGGAACAGATTTCCACGATGCACGTCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCAcc       <  1:1510740/148‑1 (MQ=255)
                                                                                                                                              tgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGcccc       >  2:1097019/1‑150 (MQ=255)
                                                                                                                                               gCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCt      >  2:1048406/1‑150 (MQ=255)
                                                                                                                                               gCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCt      >  1:445126/1‑150 (MQ=255)
                                                                                                                                                 aaaCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGa    <  2:1186076/150‑1 (MQ=255)
                                                                                                                                                  aaCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAt   >  2:1030794/1‑150 (MQ=255)
                                                                                                                                                   aCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAtt  >  1:606442/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATT  >  NC_000913/2173002‑2173298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: