Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,153 A→T 5.9% pseudogene (210/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,1530AT 5.9% 379.2 / 6.3 127pseudogene (210/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base A (66/52);  new base T (5/3);  total (71/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

CACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCC  >  NC_000913/2173004‑2173302
                                                                                                                                                     |                                                                                                                                                     
cACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaa                                                                                                                                                       <  1:1713048/150‑1 (MQ=255)
cACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaa                                                                                                                                                       <  1:1719959/150‑1 (MQ=255)
 aCCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAAt                                                                                                                                                      <  1:1454673/150‑1 (MQ=255)
    gATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGt                                                                                                                                                   >  1:1238460/1‑150 (MQ=255)
    gATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGt                                                                                                                                                   >  2:1709592/1‑150 (MQ=255)
       aataatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTg                                                                                                                                                <  2:1076550/150‑1 (MQ=255)
          aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGTGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGGTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTCTGCAAACCAATATTTTGCGt                                                                                                                                             >  2:37729/1‑150 (MQ=255)
          aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcac                                                                                                                                             <  1:766767/150‑3 (MQ=255)
          aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGt                                                                                                                                             >  1:1307046/1‑150 (MQ=255)
            tGAAACCGGGAAAATTTTGCGCTGAGAATACCTGAATAAGTAACCATGTTATGTGAGTACCGCCCTGATCCATTGAAGCCACCATACCCGCGGCTTTTAACGCCCCAGCATTATCCGCCAGTTGGGTGTGCAAACTATTGGTTTGcacac                                                                                                                                           <  1:1096105/150‑5 (MQ=255)
             gAAACCGTGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTTATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                          <  2:283408/150‑1 (MQ=255)
             gAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                          <  1:200723/150‑1 (MQ=255)
              aaaCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgcg                                                                                                                                         <  2:1789528/150‑1 (MQ=255)
                aCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAAACGCCCTGATCCATTGACGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGGGTGCAAACCAATAGGTTGCGTCGCGAt                                                                                                                                       <  2:937831/150‑1 (MQ=255)
                  cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                     <  2:749228/150‑1 (MQ=255)
                  cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                     >  1:1810317/1‑150 (MQ=255)
                  cGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc                                                                                                                                     <  1:1638622/150‑1 (MQ=255)
                         atTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGt                                                                                                                              <  1:1126358/150‑1 (MQ=255)
                           tttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGa                                                                                                                            <  1:1466995/150‑1 (MQ=255)
                            ttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGac                                                                                                                           >  2:78492/1‑149 (MQ=255)
                                  ggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCGATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCAt                                                                                                                     >  1:992969/1‑150 (MQ=255)
                                     gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAat                                                                                                                  <  1:825548/150‑1 (MQ=255)
                                     gaAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAat                                                                                                                  >  2:219167/1‑150 (MQ=255)
                                      aaatCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATTCCCCCGGCTTTTAACGCCCCATCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGGGATGCTCATAatg                                                                                                                 <  2:1810228/150‑1 (MQ=255)
                                       aaaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAACCCAATAGTTTGCGTCGCGATCCACAGGGTGCTGCTCATAatga                                                                                                                >  2:1740583/1‑150 (MQ=255)
                                        aaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCACACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgat                                                                                                               >  1:1614657/1‑150 (MQ=255)
                                        aaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgat                                                                                                               <  2:25156/150‑1 (MQ=255)
                                        aaCCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgat                                                                                                               <  2:520027/150‑1 (MQ=255)
                                         aCCTTAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAatgatg                                                                                                              <  2:1054874/150‑1 (MQ=255)
                                          ccTGAATCAGTAAGCAGGTAATGGGAGAAGCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGGATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAAtgatga                                                                                                             <  2:1016460/150‑1 (MQ=255)
                                            tGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGacc                                                                                                           >  1:1597917/1‑149 (MQ=255)
                                            tGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGaca                                                                                                           >  1:1805268/1‑150 (MQ=255)
                                                  aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATCCCCCCGGCTTTTACCGCCCCGGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGGGTCGCGATCCCCAGGGTGATGCTCTTATTGATGCCAcccatg                                                                                                     >  2:870433/1‑147 (MQ=255)
                                                  aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCgag                                                                                                     <  1:764306/150‑1 (MQ=255)
                                                  aGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCgag                                                                                                     >  2:1494757/1‑150 (MQ=255)
                                                     aaCCAGGTAATGGGAGAACCGCCATGATACATTGAAGACACCATACCCCCGGCTTTTAACGCACCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTAGCGATCCACAGGGTGATGCTAATAATGATGACACCCGAGAtt                                                                                                  >  2:1134543/1‑150 (MQ=255)
                                                      aCCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGGGATGCTCATAATGATGACACCCGATATTa                                                                                                 <  2:1314480/150‑1 (MQ=255)
                                                       ccAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTaa                                                                                                <  1:1512925/150‑1 (MQ=255)
                                                       ccAGGTAATGGGAGAACCGCCCTGATCCATCTAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTaa                                                                                                >  2:1536081/1‑150 (MQ=255)
                                                         aGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAgg                                                                                              <  1:1593606/150‑1 (MQ=255)
                                                         aGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAgg                                                                                              <  1:942924/150‑1 (MQ=255)
                                                            tAATGGGAGAACCGCCCTGATCCATTGACGCCACCAAACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGGGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGc                                                                                           <  2:1620087/150‑1 (MQ=255)
                                                             taTGGGTGATTCGCCATGATCCATTGAAGCCACCATACCCCGGGCTTTTAACTCCCCAGTATTAGCCGCCAGTTGTTTGTGCAAACCATTTGTTTGCGTCGCGATCCACAGTGTGTTGCTCTTAATTATGACACCCGAGTTTAAGGTGCg                                                                                          <  2:1540943/149‑1 (MQ=255)
                                                             aaTGGGAGAACCGCCCTGATCCAATGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTAGGGTGTGCAAAGCAATAGTTTGCGTCGCGATGCACAGGGTGATGCTCATAATGATGACACCCGAGATTAACGTGCg                                                                                          >  2:127933/1‑150 (MQ=255)
                                                               tGGGAGAACCGCCCTGATCCATTGACGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGa                                                                                        >  1:108264/1‑150 (MQ=255)
                                                                  gagaACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACa                                                                                     >  2:1443277/1‑150 (MQ=255)
                                                                      aCCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCACTAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGAtt                                                                                 <  2:913526/150‑1 (MQ=255)
                                                                        cGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTc                                                                               <  1:1589403/150‑1 (MQ=255)
                                                                         gCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTcc                                                                              >  1:1186076/1‑150 (MQ=255)
                                                                           ccTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCTCCAGTGGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCAc                                                                            <  1:846259/150‑1 (MQ=255)
                                                                            cTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGCTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACg                                                                           >  1:1414549/1‑150 (MQ=255)
                                                                              gATCCATTGAAGCCACCATACCCCCTGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGAt                                                                         <  1:219448/150‑1 (MQ=255)
                                                                                tCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGCTCCACCGGGTGTTGCTCATAATGATGACACCCGAGATTATGGAGCGGAAAAGATTTACACGATGc                                                                       >  1:901512/1‑150 (MQ=255)
                                                                                 ccATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCa                                                                      >  1:1695719/1‑150 (MQ=255)
                                                                                     tGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGc                                                                  >  2:595666/1‑150 (MQ=255)
                                                                                     tGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGc                                                                  >  2:136935/1‑150 (MQ=255)
                                                                                      gacgCCCCCATACCCCCGGCTTTTAACGCCCCAGCACTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGCGCGGAACAGATTTCCACGATGCACGGCg                                                                 <  2:959756/147‑1 (MQ=255)
                                                                                      gAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCATACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCg                                                                 >  1:753218/1‑150 (MQ=255)
                                                                                                tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                       <  2:479977/150‑1 (MQ=255)
                                                                                                tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                       >  1:603062/1‑150 (MQ=255)
                                                                                                aaCCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCg                                                       <  1:843269/149‑1 (MQ=255)
                                                                                                     ccGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACg                                                  >  1:1600713/1‑150 (MQ=255)
                                                                                                       ggCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACgaa                                                <  2:1695719/150‑1 (MQ=255)
                                                                                                        gCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCTCCACgaag                                               <  1:1295531/150‑1 (MQ=255)
                                                                                                          ttttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCCCgaagca                                             >  2:166797/1‑148 (MQ=255)
                                                                                                            ttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGc                                           >  2:511059/1‑150 (MQ=255)
                                                                                                             tAACGCCCCATAATTAGCCGCCAGTTGTGTGTGCAAACGAATAGTTTGCGTCGCGATCGACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGcc                                          <  2:866529/150‑1 (MQ=255)
                                                                                                             tAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCCCGAAGAAGcc                                          >  1:703863/1‑150 (MQ=255)
                                                                                                              aaCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCg                                         >  2:1312916/1‑150 (MQ=255)
                                                                                                               aCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCATACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCAAAATTATGAAACACGAGATTAAGGTTCTGAACAGATTTCCCCGAGGCACGGCGACCGCCATCGCCACGAAGACGCCGa                                        >  2:879058/1‑150 (MQ=255)
                                                                                                                cGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAt                                       >  1:497595/1‑150 (MQ=255)
                                                                                                                 gCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGCTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAtg                                      >  1:550504/1‑150 (MQ=255)
                                                                                                                     cAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAtggtgg                                  <  2:1132206/150‑1 (MQ=255)
                                                                                                                             gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGCTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                          <  1:389369/150‑1 (MQ=255)
                                                                                                                             gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                          >  1:745955/1‑150 (MQ=255)
                                                                                                                             gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                          >  2:610797/1‑150 (MQ=255)
                                                                                                                             gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                          <  2:1251004/150‑1 (MQ=255)
                                                                                                                             gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCg                          >  2:1343276/1‑150 (MQ=255)
                                                                                                                               cgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGcc                        >  1:738089/1‑150 (MQ=255)
                                                                                                                                    gttgggttggCAAACCAATAGTTTGCGGCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGCTTAAGGTGCGGAACAGATTTCCACGATGCATGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACg                   <  1:1121341/150‑1 (MQ=255)
                                                                                                                                    gTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAcc                   >  1:311454/1‑149 (MQ=255)
                                                                                                                                      tGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAATAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgc                 >  1:1512329/1‑150 (MQ=255)
                                                                                                                                        ggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcag               >  1:668096/1‑150 (MQ=255)
                                                                                                                                        ggTGTGCAAACCAATAGTTTGAGTCGCGATCAACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAAAAGATTTCCACGATGCACGGCGACCGCCATCGACACGAAGAAGCCGATGGTGGCAAGATAGCCAAACGgcag               >  1:455195/1‑150 (MQ=255)
                                                                                                                                         cgacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagc              >  1:1484842/5‑150 (MQ=255)
                                                                                                                                         cgacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagc              >  2:763581/5‑150 (MQ=255)
                                                                                                                                         cgacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagc              >  2:632156/5‑150 (MQ=255)
                                                                                                                                          gacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagca             >  2:1266683/4‑150 (MQ=255)
                                                                                                                                          gacgCAAACTATTGGTTTGCGTCGCGATCCACAGGGTCATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagcc             >  1:351055/4‑149 (MQ=255)
                                                                                                                                            ttcAAACCAATAGGTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGCCACCCGAGATTTAGGTGCGGAACAGATTTCCACGATGCACGTCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCAcc           <  1:1510740/148‑1 (MQ=255)
                                                                                                                                            tgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGcccc           >  2:1097019/1‑150 (MQ=255)
                                                                                                                                             gCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCt          >  2:1048406/1‑150 (MQ=255)
                                                                                                                                             gCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCt          >  1:445126/1‑150 (MQ=255)
                                                                                                                                              cAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTg         <  1:892392/150‑1 (MQ=255)
                                                                                                                                              cAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTg         <  1:1420270/150‑1 (MQ=255)
                                                                                                                                               aaaCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGa        <  2:1186076/150‑1 (MQ=255)
                                                                                                                                                aaCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAt       >  2:1030794/1‑150 (MQ=255)
                                                                                                                                                aaCCAATAGTTGGCGGCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAt       <  1:1570738/150‑1 (MQ=255)
                                                                                                                                                 aCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAtt      >  1:606442/1‑150 (MQ=255)
                                                                                                                                                    aaTAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTAcc   >  2:1564636/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCC  >  NC_000913/2173004‑2173302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: