Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,254,348 A→C 5.2% V280G (GTT→GGT)  rihB ← ribonucleoside hydrolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,254,3480AC 5.2% 346.8 / 6.2 119V280G (GTT→GGT) rihBribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base A (57/54);  new base C (2/5);  total (59/60)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 123 total aligned reads displayed.

AGCCAGGCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGCCGTAG  >  NC_000913/2254199‑2254497
                                                                                                                                                     |                                                                                                                                                     
aGCCAGGCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGaa                                                                                                                                                       <  1:888205/150‑1 (MQ=255)
aGCCAGGCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGaa                                                                                                                                                       <  1:1523313/150‑1 (MQ=255)
 gCCAGGCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAAc                                                                                                                                                      <  2:1023705/150‑1 (MQ=255)
 gCCAGGCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAAc                                                                                                                                                      >  2:310592/1‑150 (MQ=255)
  ccAGGCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACg                                                                                                                                                     <  1:1528632/150‑1 (MQ=255)
   cAGGCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGc                                                                                                                                                    >  2:129557/1‑150 (MQ=255)
     ggCCTGCATCTTGCGATCCCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGTTCTTGGCAAGCCCGcca                                                                                                                                                  <  2:157384/150‑2 (MQ=255)
     ggCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGccc                                                                                                                                                  >  1:1420958/1‑150 (MQ=255)
      gCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTACTGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCa                                                                                                                                                 <  1:533803/150‑1 (MQ=255)
       ccTTCATCTTGCGATACCGGCCCGGCTTGTTATCAACACATCATGCGCGTTGATGTAGCCGAGCACGCCCTCTTCGACTAATCCCAAGAACCATTCTGTATCAATAGTGATGCCGACTTTGGTATTGCCGGTCTTGGCAAGCCCGccaat                                                                                                                                                <  2:279544/150‑4 (MQ=255)
       ccTGCATCTTGCTATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGTTCTTGGCAAGCCCGccaat                                                                                                                                                <  2:1725592/150‑4 (MQ=255)
       ccTGCATCTTGCGATACCGGCCCGGCTTGTTATCCACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGTTCTTGGCAAGCCCGccaat                                                                                                                                                <  2:1718627/150‑4 (MQ=255)
       ccTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCCCTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATTCCGAATTTGGTATTTGCGGGATTGCCAAGCACGccaac                                                                                                                                                >  2:857586/1‑147 (MQ=255)
       ccTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAg                                                                                                                                                >  1:1389103/1‑150 (MQ=255)
       ccTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAg                                                                                                                                                >  1:243735/1‑150 (MQ=255)
       ccTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAg                                                                                                                                                >  2:850018/1‑150 (MQ=255)
          gCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGTTCTTGGCAAGCCCGccaatacc                                                                                                                                             <  1:421019/150‑7 (MQ=255)
          gCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCtc                                                                                                                                             <  2:843203/150‑1 (MQ=255)
                tGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCa                                                                                                                                       <  1:1145798/150‑1 (MQ=255)
                  cGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGa                                                                                                                                     >  2:1804918/1‑150 (MQ=255)
                    aTACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACg                                                                                                                                   <  1:728632/150‑1 (MQ=255)
                       ccggcccggcTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTa                                                                                                                                >  1:349397/1‑150 (MQ=255)
                           cccggcTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGcc                                                                                                                            >  2:1014036/1‑150 (MQ=255)
                            ccggcTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGCCTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGccc                                                                                                                           >  1:907324/1‑150 (MQ=255)
                            ccggcTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGccc                                                                                                                           <  1:1498565/150‑1 (MQ=255)
                            ccggcTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGccc                                                                                                                           >  1:1266574/1‑150 (MQ=255)
                            ccggcTTGTTATAAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCGTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGCCTTGCCAAGACCGCCAAGCTCGTCGCAGACCGTACGccc                                                                                                                           >  2:1398096/1‑150 (MQ=255)
                               gcTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATa                                                                                                                        <  1:1118458/150‑1 (MQ=255)
                                cTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAg                                                                                                                       >  2:361622/1‑150 (MQ=255)
                                 ttGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGc                                                                                                                      <  1:1271779/150‑1 (MQ=255)
                                     tATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAACAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAgg                                                                                                                  >  2:1630296/1‑150 (MQ=255)
                                      aTCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAggg                                                                                                                 >  2:1732077/1‑150 (MQ=255)
                                       tCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGc                                                                                                                <  1:1786961/150‑1 (MQ=255)
                                        cAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGcc                                                                                                               >  1:181273/1‑150 (MQ=255)
                                         aaCACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGccc                                                                                                              >  2:882239/1‑149 (MQ=255)
                                         aaCACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCa                                                                                                              >  1:206724/1‑150 (MQ=255)
                                           cacTTAATGGGTTTTGATGTAGTCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACTCCCATAGCAAGGGCCACt                                                                                                            >  1:1652030/1‑150 (MQ=255)
                                              ttAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGACCGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGtt                                                                                                         >  1:989970/1‑150 (MQ=255)
                                                 aTGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGAc                                                                                                      <  2:353214/150‑1 (MQ=255)
                                                  tGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACg                                                                                                     <  1:836842/150‑1 (MQ=255)
                                                   gggTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGt                                                                                                    <  1:129557/150‑1 (MQ=255)
                                                    ggTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTc                                                                                                   >  2:813226/1‑150 (MQ=255)
                                                     gTTTTGATGTAGCCGCGCACGCACGCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTcc                                                                                                  <  1:1412582/150‑1 (MQ=255)
                                                      ttttGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTAGACGTCCa                                                                                                 >  1:1338409/1‑150 (MQ=255)
                                                          gaggTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCATTAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTc                                                                                             <  2:645282/147‑1 (MQ=255)
                                                          gATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTc                                                                                             >  1:252081/1‑150 (MQ=255)
                                                           aTGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCg                                                                                            >  1:1257337/1‑150 (MQ=255)
                                                               aGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGt                                                                                        <  1:1577664/150‑1 (MQ=255)
                                                               aGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGAGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGt                                                                                        <  2:1817522/150‑1 (MQ=255)
                                                                gCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTa                                                                                       <  2:1471663/150‑1 (MQ=255)
                                                                  cgcgCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTTCa                                                                                     <  1:1518933/150‑1 (MQ=255)
                                                                      cacgcacTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACAtctc                                                                                 >  1:1762028/1‑150 (MQ=255)
                                                                         gcacTCTTCGACTAATCCCCAGAACCAGGCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTg                                                                              <  2:1376538/150‑1 (MQ=255)
                                                                             tctTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGtt                                                                          >  1:1046037/1‑150 (MQ=255)
                                                                                   aCTAATCCCCAGACCCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATg                                                                    <  1:715346/150‑1 (MQ=255)
                                                                                    cTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGc                                                                   >  2:30057/1‑150 (MQ=255)
                                                                                      aaTCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCa                                                                 >  2:272626/1‑150 (MQ=255)
                                                                                          cccAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAg                                                             >  1:548112/1‑150 (MQ=255)
                                                                                          cccAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAg                                                             >  1:148413/1‑150 (MQ=255)
                                                                                          cccAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGACACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAg                                                             >  2:1230042/1‑150 (MQ=255)
                                                                                             ttaaCCAGTCTGTATCAATATTGATGCCGACTTTTGTATTGGCGGGCTTGCCAAGAACGCCGAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGt                                                          <  2:460878/148‑1 (MQ=255)
                                                                                             aGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGt                                                          <  1:272626/150‑1 (MQ=255)
                                                                                             aGAACCAGTCTGTATCAATAGTGAGGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGt                                                          <  2:944468/150‑1 (MQ=255)
                                                                                               ccaaaGTCGGCATCACTATTGATACAGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTa                                                        >  1:587863/5‑150 (MQ=255)
                                                                                                 ccAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAAt                                                      >  1:370211/1‑150 (MQ=255)
                                                                                                   aGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCa                                                    <  2:1412048/150‑1 (MQ=255)
                                                                                                   aGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCa                                                    >  1:1586765/1‑150 (MQ=255)
                                                                                                   aGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGGTAATCa                                                    <  2:1297675/150‑1 (MQ=255)
                                                                                                    gTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAg                                                   >  2:531816/1‑150 (MQ=255)
                                                                                                       tGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATa                                                <  1:347905/150‑1 (MQ=255)
                                                                                                         tATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAAc                                              >  1:701446/1‑150 (MQ=255)
                                                                                                          aTCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAAcc                                             <  1:1804918/150‑1 (MQ=255)
                                                                                                           tCAATCGTTATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCTTCGCAGACGGTACGCCCATAGCAAGTGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCg                                            <  2:1779576/150‑1 (MQ=255)
                                                                                                            cAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGa                                           >  2:63463/1‑150 (MQ=255)
                                                                                                              aTAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATg                                         <  2:382107/150‑1 (MQ=255)
                                                                                                               tAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGc                                        >  1:1326110/1‑150 (MQ=255)
                                                                                                                aGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCa                                       <  1:1141211/150‑1 (MQ=255)
                                                                                                                  tGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAgg                                     <  1:1732077/150‑1 (MQ=255)
                                                                                                                    ctGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCACGGGCCACTGTTGACGTCCACTTCGACGTACAGCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTg                                   <  1:882917/149‑1 (MQ=255)
                                                                                                                       ccGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGcg                                >  2:1236719/1‑150 (MQ=255)
                                                                                                                       ccGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCAGCAGGGTTAATCAGAGAACCGATGCAGGTGGcg                                >  2:802518/1‑150 (MQ=255)
                                                                                                                         gACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGcgtc                              >  2:648734/1‑150 (MQ=255)
                                                                                                                         gACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCAACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGcgtc                              >  1:1364184/1‑150 (MQ=255)
                                                                                                                          aCTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGcgtcg                             <  1:1014036/150‑1 (MQ=255)
                                                                                                                          aCTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGcgtcg                             <  2:1420958/150‑1 (MQ=255)
                                                                                                                            tttGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTg                           <  2:206724/150‑1 (MQ=255)
                                                                                                                            tttGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGAGAACCGATGCAGGTGGCGTCGTg                           >  1:10844/1‑150 (MQ=255)
                                                                                                                             ttGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGc                          <  1:903936/150‑1 (MQ=255)
                                                                                                                              tGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCa                         >  2:955062/1‑150 (MQ=255)
                                                                                                                                    tGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGCCGTCCACTTCGACGTACATCTCCTGGGGTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCCCCGGgc                   >  2:101258/1‑150 (MQ=255)
                                                                                                                                     ggCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGgcc                  >  1:589628/1‑150 (MQ=255)
                                                                                                                                     ggCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGgcc                  <  1:1062003/150‑1 (MQ=255)
                                                                                                                                             tGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCaa          >  2:57577/1‑150 (MQ=255)
                                                                                                                                             tGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCaa          <  2:1046037/150‑1 (MQ=255)
                                                                                                                                             tGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCaa          >  1:3337/1‑150 (MQ=255)
                                                                                                                                             tGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCAACGGGCCGCCAGCaa          >  2:178881/1‑150 (MQ=255)
                                                                                                                                                cAAGATCGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGTGCCACTGGGGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGCTAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGc       <  1:1205963/150‑1 (MQ=255)
                                                                                                                                                cAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGc       <  2:1586765/150‑1 (MQ=255)
                                                                                                                                                cAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGAAGTACATCTCCTGGGGTTTAATGCCATCAGGGTTAAGCAGCTAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGc       >  2:1584488/1‑150 (MQ=255)
                                                                                                                                                     aCGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCATGTTTAATCAGATAACCGATGCAGGTGGCGGCGTGCACCGGGCCGCCAGCAAGGCCGTAg  <  2:242380/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCCAGGCCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGCCGTAG  >  NC_000913/2254199‑2254497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: