Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,279,422 A→G 5.3% A113A (GCT→GCC bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,279,4220AG 5.3% 330.9 / 6.5 114A113A (GCT→GCCbcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (60/47);  new base G (2/4);  total (63/51)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-01

Only 100 of 134 total aligned reads displayed.

AGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGA  >  NC_000913/2279274‑2279570
                                                                                                                                                    |                                                                                                                                                    
aGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcag                                                                                                                                                     >  1:1383944/1‑150 (MQ=255)
 gCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcagc                                                                                                                                                    <  1:1625102/150‑1 (MQ=255)
   tCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcagccg                                                                                                                                                  <  2:506956/150‑1 (MQ=255)
     aGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcagccgca                                                                                                                                                >  1:1189517/1‑150 (MQ=255)
      gCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTggctgcggctgcgg                                                                                                                                               >  1:1339100/1‑148 (MQ=255)
      gCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGccgcagccgcag                                                                                                                                               >  1:654324/1‑150 (MQ=255)
       ccACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGCTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCCTCAGGGCGGTAATGACCACGCTGGCcgcagccgcagc                                                                                                                                              >  1:1226544/1‑150 (MQ=255)
        cacaCCATCACCCAGCAGACAACTATCGTTGCAATCAGCTGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCgcagccgcagcc                                                                                                                                             <  1:315620/150‑1 (MQ=255)
        cacaCCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGTGCGTTAATGCCCACGCTGGCCgcagccgcagcc                                                                                                                                             >  2:510875/1‑150 (MQ=255)
          cacCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccag                                                                                                                                           <  2:182462/150‑1 (MQ=255)
            ccAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCCTCCGCGAGAACTCGTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCAGGCCGCAGCCGCagccagcc                                                                                                                                         >  2:900973/1‑150 (MQ=255)
            ccAGCACCCAGCCGCATACTATCTGGGCCATCAGCGGTGCAATGGTTGTCACCAGCTTGACAAACGACATCATCCGCGAGATCTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagcg                                                                                                                                         <  1:321714/150‑2 (MQ=255)
             cAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagcgt                                                                                                                                        <  1:575019/150‑3 (MQ=255)
              aGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTGTTTCGGGTAAATATCGCGGATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCg                                                                                                                                       >  1:675343/1‑150 (MQ=255)
              aGCAACCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCg                                                                                                                                       <  1:1588651/150‑1 (MQ=255)
                cACCCAGCCGCCAACTATCGGTGCCATCAGAGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGACCTATTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTg                                                                                                                                     >  2:267499/1‑150 (MQ=255)
                 aCCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCCTCCGCGAGAACGCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTgg                                                                                                                                    <  1:1216181/150‑1 (MQ=255)
                  cccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                                   >  2:130467/1‑150 (MQ=255)
                  cccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                                   >  2:1762261/1‑150 (MQ=255)
                   ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaa                                                                                                                                  <  2:1491390/150‑1 (MQ=255)
                     aGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaaga                                                                                                                                >  1:1034759/1‑150 (MQ=255)
                     aGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaaga                                                                                                                                >  1:1004392/1‑150 (MQ=255)
                        cgccAACTATCGGTTCCATCAGCGGTGCAATGGTTGTCACCAGAATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTTGCCGCAGCCGCAGCCAGCCCGTGGAAGAAAc                                                                                                                             <  1:537348/150‑1 (MQ=255)
                         gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCGGCCGCAGCCAGCCCGTGGAAGAAACg                                                                                                                            >  2:1631201/1‑150 (MQ=255)
                         gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACg                                                                                                                            >  1:719634/1‑150 (MQ=255)
                         gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACg                                                                                                                            >  2:241076/1‑150 (MQ=255)
                         gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACg                                                                                                                            >  2:328160/1‑150 (MQ=255)
                           cAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTATTGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCa                                                                                                                          >  2:1624117/1‑150 (MQ=255)
                            aaCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGGTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCAt                                                                                                                         <  2:1746429/150‑1 (MQ=255)
                               tATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATcac                                                                                                                      <  1:626137/150‑1 (MQ=255)
                                 tCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACaa                                                                                                                    <  2:1536951/150‑1 (MQ=255)
                                    gTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCa                                                                                                                 <  2:866696/150‑1 (MQ=255)
                                        cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTg                                                                                                             >  1:1543410/1‑150 (MQ=255)
                                        cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTg                                                                                                             >  1:1672561/1‑150 (MQ=255)
                                        cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTg                                                                                                             >  2:228941/1‑150 (MQ=255)
                                            aGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCg                                                                                                         <  1:1591100/150‑1 (MQ=255)
                                                 tGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGgt                                                                                                    >  2:1510302/1‑150 (MQ=255)
                                                    aaTGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGtt                                                                                                 >  1:1332348/1‑150 (MQ=255)
                                                      tGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTg                                                                                               <  1:1080901/150‑1 (MQ=255)
                                                      tGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTg                                                                                               <  2:1404059/150‑1 (MQ=255)
                                                        gTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGAGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGcc                                                                                             <  1:1389499/150‑1 (MQ=255)
                                                            tCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCCACg                                                                                         >  1:1358260/1‑150 (MQ=255)
                                                            tCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGCGTAAATATCGCGCATCATGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACg                                                                                         <  1:1517391/150‑1 (MQ=255)
                                                             cACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGc                                                                                        >  2:841123/1‑150 (MQ=255)
                                                                cAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGcaca                                                                                     >  1:1060206/1‑150 (MQ=255)
                                                                  gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACg                                                                                   <  1:1762261/150‑1 (MQ=255)
                                                                        cAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcg                                                                             >  1:882163/1‑150 (MQ=255)
                                                                        cAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGGGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcg                                                                             >  2:1168099/1‑150 (MQ=255)
                                                                         aaaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcgc                                                                            >  2:1225894/1‑150 (MQ=255)
                                                                          aaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTTTTTGCCAACGCACACGCCCCcgcg                                                                           >  2:137833/1‑150 (MQ=255)
                                                                                 tcatcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGcggcggcgg                                                                    <  1:458515/150‑1 (MQ=255)
                                                                                 tcatcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGcggcggcgg                                                                    <  2:1149887/150‑1 (MQ=255)
                                                                                      cgcgAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAac                                                               <  2:427825/150‑1 (MQ=255)
                                                                                      cgcgAGAACTCTACTTTCGGGTAAATATCGCGCATCAGTGCGTTAATGACCACGCTCGCCGCAGCCGCAGCCAGCCCGTGGAAGAACCGCATCACACTCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAac                                                               <  1:1229310/150‑1 (MQ=255)
                                                                                       gcgAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAaca                                                              <  1:1221063/150‑1 (MQ=255)
                                                                                        cgAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAacac                                                             <  1:1021028/150‑1 (MQ=255)
                                                                                             aCTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCg                                                        <  2:674895/150‑1 (MQ=255)
                                                                                                 ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTAcc                                                    >  2:1111783/1‑150 (MQ=255)
                                                                                                   cttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTAccgc                                                  >  2:1781736/1‑150 (MQ=255)
                                                                                                    tttCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTAccgcc                                                 >  2:1302985/1‑150 (MQ=255)
                                                                                                       cGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAg                                              >  1:1433574/1‑150 (MQ=255)
                                                                                                       cGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAg                                              >  2:214807/1‑150 (MQ=255)
                                                                                                       cGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAg                                              <  1:442548/150‑1 (MQ=255)
                                                                                                       cGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAg                                              >  1:1659358/1‑150 (MQ=255)
                                                                                                        gggTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCCGCTGATCGATGGTGTTTGCCAACGCACCCGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGc                                             >  1:276450/1‑150 (MQ=255)
                                                                                                        gggTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCgcgc                                             >  1:412824/1‑150 (MQ=255)
                                                                                                        gggTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGc                                             >  1:1384818/1‑150 (MQ=255)
                                                                                                         ggTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAACTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGca                                            <  2:1189517/150‑1 (MQ=255)
                                                                                                               tatCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCCGCCGCAGCCAGCCCGGGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACCCGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGCGCACCACCg                                      >  2:913431/1‑150 (MQ=255)
                                                                                                                atCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCgg                                     <  2:1332348/150‑1 (MQ=255)
                                                                                                                atCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACAGCCGAGCACCACCgg                                     >  1:1442121/1‑150 (MQ=255)
                                                                                                                atCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGGTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCgg                                     <  1:491336/150‑1 (MQ=255)
                                                                                                                   gcgcATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCtt                                  <  2:719634/150‑1 (MQ=255)
                                                                                                                        tCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCCCACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGCGCACCACCGGCTTCCGcc                             >  1:1749750/1‑150 (MQ=255)
                                                                                                                        tCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGcc                             <  1:872439/150‑1 (MQ=255)
                                                                                                                         cAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGCGCACCACCGGCTTACGccc                            >  1:224981/1‑150 (MQ=255)
                                                                                                                         cAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGccc                            <  2:1738364/150‑1 (MQ=255)
                                                                                                                          aGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCCCCGCGGCGGCGGCCAACACCCGCGTACCGCCGAGCACCACCGGCTTACGCCCg                           >  1:234105/1‑150 (MQ=255)
                                                                                                                           gggCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCCCCGGCTTACGCCCGa                          >  2:104835/1‑150 (MQ=255)
                                                                                                                             gCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAg                        <  1:678680/150‑1 (MQ=255)
                                                                                                                             gCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAg                        <  2:38239/150‑1 (MQ=255)
                                                                                                                                ttAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGCAGCTg                     >  1:1493244/1‑150 (MQ=255)
                                                                                                                                 tAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCCCCACCGGCTTACGCCCGAAGCTGt                    >  1:1727973/1‑150 (MQ=255)
                                                                                                                                      aCCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGcc               >  2:151973/1‑150 (MQ=255)
                                                                                                                                       ccACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAACCACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCa              >  2:461228/1‑150 (MQ=255)
                                                                                                                                       ccACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCa              <  1:130467/150‑1 (MQ=255)
                                                                                                                                        cACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGCGCACCACCGGCTTACGCCCGAAGCTGTCTGCCAt             >  1:813424/1‑150 (MQ=255)
                                                                                                                                          ctcTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTTCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCg           <  2:884102/148‑1 (MQ=255)
                                                                                                                                             tGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGcc        <  1:1431703/150‑1 (MQ=255)
                                                                                                                                             gggCAGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGCTTGCCAACGAACACGCCACCGCGGCGGTGGCAAACACCAGCGTACCGACGAGCACCACCGGCTTACGCCCGAAGCTTTCTGCCATCGGcc        <  1:253643/149‑1 (MQ=255)
                                                                                                                                               gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCg      <  2:1548990/150‑1 (MQ=255)
                                                                                                                                               gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCg      <  2:1007915/150‑1 (MQ=255)
                                                                                                                                               gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGATGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCg      <  1:2006/150‑1 (MQ=255)
                                                                                                                                                ccgcagccgcagCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGt     <  1:841123/150‑1 (MQ=255)
                                                                                                                                                  gcagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAg   <  1:1510302/150‑1 (MQ=255)
                                                                                                                                                  gcagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAg   <  2:654324/150‑1 (MQ=255)
                                                                                                                                                  gcagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAg   <  1:31392/150‑1 (MQ=255)
                                                                                                                                                  gcagccgcagccAGCCCGTCGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACCCCAGCGTACCGCCGAGCCCCCCCGGCTTACGCCCGAAGCGGTCTGCCATCGGccccgag   >  1:82797/1‑146 (MQ=255)
                                                                                                                                                   cagccgcagccAGCCCTTGGAAGAAACGCATCACAATCTGCTGATCGATGGAGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGa  <  1:267499/150‑1 (MQ=255)
                                                                                                                                                   cagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGa  <  1:1081340/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGA  >  NC_000913/2279274‑2279570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: