Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,449 A→G 5.7% intergenic (+57/‑68) yejH → / → rplY putative ATP‑dependent DNA or RNA helicase/50S ribosomal subunit protein L25

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,4490AG 5.7% 315.2 / 6.7 105intergenic (+57/‑68)yejH/rplYputative ATP‑dependent DNA or RNA helicase/50S ribosomal subunit protein L25
Reads supporting (aligned to +/- strand):  ref base A (47/51);  new base G (4/2);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 105 total aligned reads displayed.

CGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCG  >  NC_000913/2282302‑2282597
                                                                                                                                                   |                                                                                                                                                     
gtcccTCATGAAAGGCCTGTACTGGCAGGTGCGTGATAAAGTGTTCGATTACTAAGGTCGTTTTCTTCTGGAGCACTAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATa                                                                                                                                                     <  1:330248/148‑1 (MQ=255)
   ccGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGc                                                                                                                                                  <  2:805793/150‑1 (MQ=255)
    cGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCa                                                                                                                                                <  1:683337/150‑1 (MQ=255)
    cGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCa                                                                                                                                                <  1:718161/150‑1 (MQ=255)
    cGCATGAAAGGCCAGTACTGGAAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCa                                                                                                                                                >  1:1201283/1‑150 (MQ=255)
        tGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGc                                                                                                                                            <  1:648844/150‑1 (MQ=255)
        tGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGc                                                                                                                                            >  2:818105/1‑150 (MQ=255)
          aaaGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAg                                                                                                                                          >  2:679645/1‑150 (MQ=255)
           aaGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGa                                                                                                                                         <  2:1484148/150‑1 (MQ=255)
            aGGCCAGTACTGGCAGGTGCGTGAAAAACTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCGTATGCaaaagcggn                                                                                                                                        <  2:56020/150‑7 (MQ=255)
             ggCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATc                                                                                                                                       <  1:400562/150‑1 (MQ=255)
             ggCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATc                                                                                                                                       >  1:1277715/1‑150 (MQ=255)
             ggCCAGTACTGGCAGGGGCGAGAAAAAGTGTTCGATTACGAAGGGCGGTTTCGTCGGGCGCACGAATTTCGCGGTTAATGGTAGTTTTGATTGAGGTATAAGGCGATTGAGTAAAGAATCTCGCCCGCTTTTGCGTATGCaaaagcgggc                                                                                                                                       <  1:1563460/150‑8 (MQ=255)
              gCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCa                                                                                                                                      <  2:1578885/150‑1 (MQ=255)
                cAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACt                                                                                                                                    >  2:558592/1‑150 (MQ=255)
                  gTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTa                                                                                                                                  >  2:886619/1‑150 (MQ=255)
                    aCTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGAATGATGTATAAGGCGATTGAGTTTAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTAcc                                                                                                                                <  2:1703325/150‑1 (MQ=255)
                     cTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCACTTTTGCATACGCAAAGCAGATCACTTACCt                                                                                                                               >  1:1141823/1‑150 (MQ=255)
                       ggCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGt                                                                                                                             >  1:1539475/1‑150 (MQ=255)
                        gCAGGTGCGTGATAAAGTGTTCGTTTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTATTGGTAGTTTTGATTTATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGtt                                                                                                                            <  1:1170047/150‑1 (MQ=255)
                         cAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTg                                                                                                                           <  1:1210942/150‑1 (MQ=255)
                         cAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTg                                                                                                                           >  2:773966/1‑150 (MQ=255)
                            gTGCGTGAAAAAGTGTTCGATTACGTAGGTCGTTTTCGTCTGGCGCACGATATACGCGGTTAATGGTAGTTTTGATTGATGTATTAGGCGATTGAGTATAGTATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTg                                                                                                                        <  1:301042/150‑1 (MQ=255)
                               cGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCACGCTTTTGCATACGCAAAGCAGATCACTTAACTGTTGCTGGGt                                                                                                                     >  1:1119658/1‑150 (MQ=255)
                                   aaaaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGcc                                                                                                                 <  1:1232559/150‑1 (MQ=255)
                                    aaaaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCt                                                                                                                >  1:772011/1‑150 (MQ=255)
                                       aGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGGTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTa                                                                                                             <  1:1315807/150‑1 (MQ=255)
                                           ttCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAg                                                                                                         <  1:1389543/150‑1 (MQ=255)
                                            tCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAgg                                                                                                        <  2:844569/150‑1 (MQ=255)
                                             ggATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGTTTGATGTATAAGGCGATTGAGTATAGTATCTCGCCCGCTTTTGCATACGCAATGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGa                                                                                                       <  1:10771/149‑1 (MQ=255)
                                             cGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGa                                                                                                       >  2:1693751/1‑150 (MQ=255)
                                              gATTACGAAGGTCGTTTTCGTCTGGCGCTCGAATTACTCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTAGTGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGAt                                                                                                      <  1:113634/150‑1 (MQ=255)
                                                ttACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTa                                                                                                    >  1:396460/1‑150 (MQ=255)
                                                 tACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTaa                                                                                                   >  1:10937/1‑150 (MQ=255)
                                                   cGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAAtt                                                                                                 <  1:230604/150‑1 (MQ=255)
                                                    gAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAAttt                                                                                                <  2:493441/150‑1 (MQ=255)
                                                     aaGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTa                                                                                               >  1:453188/1‑150 (MQ=255)
                                                     aaGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTa                                                                                               >  2:1525241/1‑150 (MQ=255)
                                                       ggTCTTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAag                                                                                             <  1:1432730/150‑1 (MQ=255)
                                                       ggTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAGTTTAag                                                                                             <  2:757188/150‑1 (MQ=255)
                                                         tagTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAagag                                                                                           <  2:1165494/148‑1 (MQ=255)
                                                         tCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAagag                                                                                           <  2:1262126/150‑1 (MQ=255)
                                                          cGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAagaga                                                                                          >  2:1468395/1‑150 (MQ=255)
                                                               tCGTCTGGCGCACGAATTACGCTGTTAATGGTAGTTTTGAATGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGagaaag                                                                                     <  2:149808/150‑1 (MQ=255)
                                                                 gTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGagaaagaa                                                                                   <  2:466710/150‑1 (MQ=255)
                                                                  tCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAgaaagaaa                                                                                  <  1:557637/150‑1 (MQ=255)
                                                                      gcgcACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGtt                                                                              >  1:1156335/1‑150 (MQ=255)
                                                                        gcACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTa                                                                            >  2:826605/1‑150 (MQ=255)
                                                                         cACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGAGATTGATTATAGAATCTCGCCCGCTTTTGCATACGCAAATCAGATCTCTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAATAGAGAAAGAAATGTTTAc                                                                           <  1:911882/150‑1 (MQ=255)
                                                                            gAATTACGCGGTTTATGGTAGTTTTGATTGATGTATAAGGCGATTGATTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTAt                                                                        <  1:1341431/150‑1 (MQ=255)
                                                                             aaTTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATc                                                                       >  1:908253/1‑150 (MQ=255)
                                                                               ttACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGGCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCac                                                                     >  1:775264/1‑149 (MQ=255)
                                                                                 aCGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACg                                                                   <  2:1670305/150‑1 (MQ=255)
                                                                                  cgcgGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGc                                                                  <  2:1373117/150‑1 (MQ=255)
                                                                                     ggTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGa                                                               <  1:460913/150‑1 (MQ=255)
                                                                                      gTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGaa                                                              >  2:750014/1‑150 (MQ=255)
                                                                                       ttAATGGTAGTCTTGATTGATTTATAATGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTATCTGTTTCTGGGTCGCCTGTATCAGGATTAATTTAAGATAGAAAGAAATGTTTACTATCAACGCAGAAg                                                             <  2:1101935/150‑1 (MQ=255)
                                                                                         aaTGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTa                                                           >  1:660012/1‑150 (MQ=255)
                                                                                          aTGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTAc                                                          <  2:1665398/150‑1 (MQ=255)
                                                                                           tGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACg                                                         >  2:598102/1‑150 (MQ=255)
                                                                                              tAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTaa                                                      >  2:1061503/1‑150 (MQ=255)
                                                                                                 ttttGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAaga                                                   >  1:1102270/1‑150 (MQ=255)
                                                                                                   ttgattgaTGTATAAGGCGATTGAGTATAGAAGCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGc                                                 <  2:1285124/150‑1 (MQ=255)
                                                                                                      tttgatGTATAAGGCGATTGAGTATATAATCTCGCCAGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAgg                                              <  2:291239/149‑1 (MQ=255)
                                                                                                       ttgatGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCTGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGTAAGAAATGTTTACTTTCATCGCTGAAGTACGTTAAGAGCAggg                                             >  1:194285/1‑150 (MQ=255)
                                                                                                        tgatGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGt                                            >  2:1780899/1‑150 (MQ=255)
                                                                                                         gatGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGAAGAAGTACGTAAAGAGCAGGGTa                                           >  1:1163555/1‑150 (MQ=255)
                                                                                                           tGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAg                                         >  1:926940/1‑150 (MQ=255)
                                                                                                            gTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACATGTTGCTGGGTCGCCTGTAGAAGGATTAATTTAAGATAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAgg                                        >  1:1596135/1‑150 (MQ=255)
                                                                                                             tataAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAggg                                       <  2:660012/150‑1 (MQ=255)
                                                                                                             tataAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCAATTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAggg                                       <  2:117062/150‑1 (MQ=255)
                                                                                                               taAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTTCCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTg                                     <  2:1632578/150‑1 (MQ=255)
                                                                                                               taAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTg                                     >  1:381452/1‑150 (MQ=255)
                                                                                                                 aGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCg                                   >  1:773328/1‑150 (MQ=255)
                                                                                                                   gCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAg                                 <  1:444338/150‑1 (MQ=255)
                                                                                                                    cGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCCCTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgc                                >  1:1118051/1‑150 (MQ=255)
                                                                                                                    cGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgc                                <  1:728047/150‑1 (MQ=255)
                                                                                                                      atggAGTTTTGAATCTCGCCCGGTTTTGGATTCGCAAAGGAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCTGGATTAATTTAAGAGAGAAAGAATTGTTTACTATCATCGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgccg                              <  2:1165527/147‑1 (MQ=255)
                                                                                                                      aTTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgccg                              <  1:886619/150‑1 (MQ=255)
                                                                                                                       ttGAGTATAGAATCTCGCCCGCTTTTGCCTACGCAAAGCAGCTCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGAGTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgccgc                             >  1:300788/1‑150 (MQ=255)
                                                                                                                         gAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATATAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgccgccg                           >  1:186685/1‑150 (MQ=255)
                                                                                                                             ataGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTg                       <  2:782476/150‑1 (MQ=255)
                                                                                                                             ataGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGAAGGGTAAGGGTGCGAGACGCCGCCTg                       >  2:312139/1‑150 (MQ=255)
                                                                                                                                gAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGTGTAAGGGTGCGAGCCGCCGCCTGCGt                    <  1:773966/150‑1 (MQ=255)
                                                                                                                                 aaTCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTg                   <  1:1693751/150‑1 (MQ=255)
                                                                                                                                     tcGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGc               <  2:772011/150‑1 (MQ=255)
                                                                                                                                       gCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGTTGCGAGCCGCCGCCTGCCTGCCGCTa             >  2:228509/1‑150 (MQ=255)
                                                                                                                                       gCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa             >  1:127878/1‑150 (MQ=255)
                                                                                                                                       gCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa             >  2:788108/1‑150 (MQ=255)
                                                                                                                                       gCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGCTCGCCTGTAGCAGCATTAATATAAGAGAGAAAGAAATGTAAACAATCAACGCAGAAGTACGTAAAGAGAAGGGAAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa             >  2:223099/1‑150 (MQ=255)
                                                                                                                                        cccGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTaa            >  1:1646268/1‑150 (MQ=255)
                                                                                                                                        atctgctttGCGTATGCAAAAGCAGATCAGTTACCTGTTGCTGGGTCGCCTGTAGCTGGATTAATTTAAGAGAGAAAGAAATGGTTACTATCAACGCAGAAGTACGTAAAGCGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa             >  1:80940/7‑150 (MQ=255)
                                                                                                                                        atctgctttGCGTATGCAAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa             >  1:1244587/7‑150 (MQ=255)
                                                                                                                                          cGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACa          <  2:1418809/150‑1 (MQ=255)
                                                                                                                                            gctttGCGTATGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGACAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAg        >  2:106080/3‑150 (MQ=255)
                                                                                                                                              tttGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGtt      >  1:648574/1‑150 (MQ=255)
                                                                                                                                                tGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTACGTGCCACTAACAAGTTcc    <  2:1539475/150‑1 (MQ=255)
                                                                                                                                                  cATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGCAGTACGTAAAGCGCAGGGTAAGGGTCCGCGCCGCCGCCTGCGGCCCGCTACCAAGTTCCCg  >  2:1444122/1‑150 (MQ=255)
                                                                                                                                                  cATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCg  >  1:933712/1‑150 (MQ=255)
                                                                                                                                                  cATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCg  >  2:1581696/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCG  >  NC_000913/2282302‑2282597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: