Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,452 C→T 5.5% intergenic (+60/‑65) yejH → / → rplY putative ATP‑dependent DNA or RNA helicase/50S ribosomal subunit protein L25

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,4520CT 5.5% 343.1 / 6.0 108intergenic (+60/‑65)yejH/rplYputative ATP‑dependent DNA or RNA helicase/50S ribosomal subunit protein L25
Reads supporting (aligned to +/- strand):  ref base C (50/52);  new base T (4/2);  total (54/54)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

Only 100 of 109 total aligned reads displayed.

CCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGCAA  >  NC_000913/2282305‑2282601
                                                                                                                                                   |                                                                                                                                                      
ccGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGc                                                                                                                                                      <  2:805793/150‑1 (MQ=255)
 cGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCa                                                                                                                                                    <  1:718161/150‑1 (MQ=255)
 cGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCa                                                                                                                                                    <  1:683337/150‑1 (MQ=255)
 cGCATGAAAGGCCAGTACTGGAAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCa                                                                                                                                                    >  1:1201283/1‑150 (MQ=255)
     tGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGc                                                                                                                                                <  1:648844/150‑1 (MQ=255)
     tGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGc                                                                                                                                                >  2:818105/1‑150 (MQ=255)
        aaGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGa                                                                                                                                             <  2:1484148/150‑1 (MQ=255)
         aGGCCAGTACTGGCAGGTGCGTGAAAAACTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCGTATGCaaaagcggn                                                                                                                                            <  2:56020/150‑7 (MQ=255)
          ggCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATc                                                                                                                                           <  1:400562/150‑1 (MQ=255)
          ggCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATc                                                                                                                                           >  1:1277715/1‑150 (MQ=255)
          ggCCAGTACTGGCAGGGGCGAGAAAAAGTGTTCGATTACGAAGGGCGGTTTCGTCGGGCGCACGAATTTCGCGGTTAATGGTAGTTTTGATTGAGGTATAAGGCGATTGAGTAAAGAATCTCGCCCGCTTTTGCGTATGCaaaagcgggc                                                                                                                                           <  1:1563460/150‑8 (MQ=255)
           gCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCa                                                                                                                                          <  2:1578885/150‑1 (MQ=255)
             cAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACt                                                                                                                                        >  2:558592/1‑150 (MQ=255)
               gTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTa                                                                                                                                      >  2:886619/1‑150 (MQ=255)
                 aCTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGAATGATGTATAAGGCGATTGAGTTTAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTAcc                                                                                                                                    <  2:1703325/150‑1 (MQ=255)
                  cTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCACTTTTGCATACGCAAAGCAGATCACTTACCt                                                                                                                                   >  1:1141823/1‑150 (MQ=255)
                    ggCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGt                                                                                                                                 >  1:1539475/1‑150 (MQ=255)
                     gCAGGTGCGTGATAAAGTGTTCGTTTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTATTGGTAGTTTTGATTTATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGtt                                                                                                                                <  1:1170047/150‑1 (MQ=255)
                      cAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTg                                                                                                                               >  2:773966/1‑150 (MQ=255)
                      cAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTg                                                                                                                               <  1:1210942/150‑1 (MQ=255)
                         gTGCGTGAAAAAGTGTTCGATTACGTAGGTCGTTTTCGTCTGGCGCACGATATACGCGGTTAATGGTAGTTTTGATTGATGTATTAGGCGATTGAGTATAGTATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTg                                                                                                                            <  1:301042/150‑1 (MQ=255)
                            cGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCACGCTTTTGCATACGCAAAGCAGATCACTTAACTGTTGCTGGGt                                                                                                                         >  1:1119658/1‑150 (MQ=255)
                                   aaGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGt                                                                                                                  >  1:1464527/1‑150 (MQ=255)
                                    aGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGGTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTa                                                                                                                 <  1:1315807/150‑1 (MQ=255)
                                        ttCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAg                                                                                                             <  1:1389543/150‑1 (MQ=255)
                                         tCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAgg                                                                                                            <  2:844569/150‑1 (MQ=255)
                                          ggATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGTTTGATGTATAAGGCGATTGAGTATAGTATCTCGCCCGCTTTTGCATACGCAATGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGa                                                                                                           <  1:10771/149‑1 (MQ=255)
                                          cGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGa                                                                                                           >  2:1693751/1‑150 (MQ=255)
                                           gATTACGAAGGTCGTTTTCGTCTGGCGCTCGAATTACTCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTAGTGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGAt                                                                                                          <  1:113634/150‑1 (MQ=255)
                                             ttACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTa                                                                                                        >  1:396460/1‑150 (MQ=255)
                                              tACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTaa                                                                                                       >  1:10937/1‑150 (MQ=255)
                                                cGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAAtt                                                                                                     <  1:230604/150‑1 (MQ=255)
                                                cGAAGGTCGTTTTCGTCTGGCGCACGAATTACACGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAAtt                                                                                                     >  2:269070/1‑150 (MQ=255)
                                                 gAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAAttt                                                                                                    <  2:493441/150‑1 (MQ=255)
                                                  aaGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTa                                                                                                   >  1:453188/1‑150 (MQ=255)
                                                  aaGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTa                                                                                                   >  2:1525241/1‑150 (MQ=255)
                                                    ggTCTTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAag                                                                                                 <  1:1432730/150‑1 (MQ=255)
                                                    ggTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAGTTTAag                                                                                                 <  2:757188/150‑1 (MQ=255)
                                                      tagTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAagag                                                                                               <  2:1165494/148‑1 (MQ=255)
                                                      tCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAagag                                                                                               <  2:1262126/150‑1 (MQ=255)
                                                       cGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAagaga                                                                                              >  2:1468395/1‑150 (MQ=255)
                                                            tCGTCTGGCGCACGAATTACGCTGTTAATGGTAGTTTTGAATGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGagaaag                                                                                         <  2:149808/150‑1 (MQ=255)
                                                             cGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCTCTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGagaaaga                                                                                        <  2:1201283/150‑1 (MQ=255)
                                                              gTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGagaaagaa                                                                                       <  2:466710/150‑1 (MQ=255)
                                                               tCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAgaaagaaa                                                                                      <  1:557637/150‑1 (MQ=255)
                                                                   gcgcACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGtt                                                                                  >  1:1156335/1‑150 (MQ=255)
                                                                     gcACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTa                                                                                >  2:826605/1‑150 (MQ=255)
                                                                      cACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGAGATTGATTATAGAATCTCGCCCGCTTTTGCATACGCAAATCAGATCTCTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAATAGAGAAAGAAATGTTTAc                                                                               <  1:911882/150‑1 (MQ=255)
                                                                         gAATTACGCGGTTTATGGTAGTTTTGATTGATGTATAAGGCGATTGATTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTAt                                                                            <  1:1341431/150‑1 (MQ=255)
                                                                          aaTTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATc                                                                           >  1:908253/1‑150 (MQ=255)
                                                                            ttACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGGCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCac                                                                         >  1:775264/1‑149 (MQ=255)
                                                                              aCGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACg                                                                       <  2:1670305/150‑1 (MQ=255)
                                                                               cgcgGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGc                                                                      <  2:1373117/150‑1 (MQ=255)
                                                                                  ggTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGa                                                                   <  1:460913/150‑1 (MQ=255)
                                                                                   gTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGaa                                                                  >  2:750014/1‑150 (MQ=255)
                                                                                      aaTGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTa                                                               >  1:660012/1‑150 (MQ=255)
                                                                                       aTGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTAc                                                              <  2:1665398/150‑1 (MQ=255)
                                                                                        tGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACg                                                             >  2:598102/1‑150 (MQ=255)
                                                                                           tAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTaa                                                          >  2:1061503/1‑150 (MQ=255)
                                                                                              ttttGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAaga                                                       >  1:1102270/1‑150 (MQ=255)
                                                                                                ttgattgaTGTATAAGGCGATTGAGTATAGAAGCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGc                                                     <  2:1285124/150‑1 (MQ=255)
                                                                                                   tttgatGTATAAGGCGATTGAGTATATAATCTCGCCAGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAgg                                                  <  2:291239/149‑1 (MQ=255)
                                                                                                    ttgatGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCTGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGTAAGAAATGTTTACTTTCATCGCTGAAGTACGTTAAGAGCAggg                                                 >  1:194285/1‑150 (MQ=255)
                                                                                                     tgatGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGt                                                >  2:1780899/1‑150 (MQ=255)
                                                                                                      gatGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGAAGAAGTACGTAAAGAGCAGGGTa                                               >  1:1163555/1‑150 (MQ=255)
                                                                                                        tGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAg                                             >  1:926940/1‑150 (MQ=255)
                                                                                                         gTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACATGTTGCTGGGTCGCCTGTAGAAGGATTAATTTAAGATAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAgg                                            >  1:1596135/1‑150 (MQ=255)
                                                                                                          tataAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAggg                                           <  2:660012/150‑1 (MQ=255)
                                                                                                          tataAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCAATTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAggg                                           <  2:117062/150‑1 (MQ=255)
                                                                                                            taAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTTCCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTg                                         <  2:1632578/150‑1 (MQ=255)
                                                                                                            taAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTg                                         >  1:381452/1‑150 (MQ=255)
                                                                                                              aGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCg                                       >  1:773328/1‑150 (MQ=255)
                                                                                                                 cGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCCCTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgc                                    >  1:1118051/1‑150 (MQ=255)
                                                                                                                 cGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgc                                    <  1:728047/150‑1 (MQ=255)
                                                                                                                   atggAGTTTTGAATCTCGCCCGGTTTTGGATTCGCAAAGGAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCTGGATTAATTTAAGAGAGAAAGAATTGTTTACTATCATCGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgccg                                  <  2:1165527/147‑1 (MQ=255)
                                                                                                                   aTTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgccg                                  <  1:886619/150‑1 (MQ=255)
                                                                                                                    ttGAGTATAGAATCTCGCCCGCTTTTGCCTACGCAAAGCAGCTCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGAGTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgccgc                                 >  1:300788/1‑150 (MQ=255)
                                                                                                                      gAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATATAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAgccgccg                               >  1:186685/1‑150 (MQ=255)
                                                                                                                          ataGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTg                           <  2:782476/150‑1 (MQ=255)
                                                                                                                          ataGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGAAGGGTAAGGGTGCGAGACGCCGCCTg                           >  2:312139/1‑150 (MQ=255)
                                                                                                                             gAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGTGTAAGGGTGCGAGCCGCCGCCTGCGt                        <  1:773966/150‑1 (MQ=255)
                                                                                                                              aaTCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTg                       <  1:1693751/150‑1 (MQ=255)
                                                                                                                                  tcGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGc                   <  2:772011/150‑1 (MQ=255)
                                                                                                                                    gCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGTTGCGAGCCGCCGCCTGCCTGCCGCTa                 >  2:228509/1‑150 (MQ=255)
                                                                                                                                    gCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa                 >  2:788108/1‑150 (MQ=255)
                                                                                                                                    gCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa                 >  1:127878/1‑150 (MQ=255)
                                                                                                                                     atctgctttGCGTATGCAAAAGCAGATCAGTTACCTGTTGCTGGGTCGCCTGTAGCTGGATTAATTTAAGAGAGAAAGAAATGGTTACTATCAACGCAGAAGTACGTAAAGCGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa                 >  1:80940/7‑150 (MQ=255)
                                                                                                                                     atctgctttGCGTATGCAAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTa                 >  1:1244587/7‑150 (MQ=255)
                                                                                                                                       ctgctttGCGTATGCAAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAAc               >  1:649390/5‑150 (MQ=255)
                                                                                                                                       cGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACa              <  2:1418809/150‑1 (MQ=255)
                                                                                                                                         gctttGCGTATGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGACAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAg            >  2:106080/3‑150 (MQ=255)
                                                                                                                                           tttGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGtt          >  1:648574/1‑150 (MQ=255)
                                                                                                                                             tGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTACGTGCCACTAACAAGTTcc        <  2:1539475/150‑1 (MQ=255)
                                                                                                                                               cATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGCAGTACGTAAAGCGCAGGGTAAGGGTCCGCGCCGCCGCCTGCGGCCCGCTACCAAGTTCCCg      >  2:1444122/1‑150 (MQ=255)
                                                                                                                                               cATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCg      >  1:933712/1‑150 (MQ=255)
                                                                                                                                               cATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCg      >  2:1581696/1‑150 (MQ=255)
                                                                                                                                                 tACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGc    >  1:1808004/1‑150 (MQ=255)
                                                                                                                                                 aaCGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGc    <  2:578121/149‑1 (MQ=255)
                                                                                                                                                 aaCGCAAAGCAGATCACTTACCTGTTGCTGGGGCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGc    <  1:55025/149‑1 (MQ=255)
                                                                                                                                                   cGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGCaa  >  1:1748405/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATGGTAGTTTTGATTGATGTATAAGGCGATTGAGTATAGAATCTCGCCCGCTTTTGCATACGCAAAGCAGATCACTTACCTGTTGCTGGGTCGCCTGTAGCAGGATTAATTTAAGAGAGAAAGAAATGTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGCAA  >  NC_000913/2282305‑2282601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: