Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,290,448 A→G 5.1% intergenic (‑268/‑52) yejO ← / → narP pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,290,4480AG 5.1% 424.9 / 9.1 137intergenic (‑268/‑52)yejO/narPpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ
Reads supporting (aligned to +/- strand):  ref base A (65/65);  new base G (3/4);  total (68/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

Only 100 of 138 total aligned reads displayed.

ATTGATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTG  >  NC_000913/2290299‑2290596
                                                                                                                                                     |                                                                                                                                                    
attgattTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTa                                                                                                                                                      >  1:1824490/1‑150 (MQ=255)
   tattTTTTAGTTGTTTTTCTTGATGAGAAGATTATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGCAGTTTTCACTACATGTGCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCa                                                                                                                                                   <  2:1169668/149‑1 (MQ=255)
   gattTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCa                                                                                                                                                   >  1:513797/1‑150 (MQ=255)
   gattTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCa                                                                                                                                                   <  1:432311/150‑1 (MQ=255)
     ttttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAac                                                                                                                                                 >  1:725724/1‑150 (MQ=255)
     ttttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCAATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAac                                                                                                                                                 >  2:1693635/1‑150 (MQ=255)
      tttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacg                                                                                                                                                >  1:1341727/1‑150 (MQ=255)
       ttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCGATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgc                                                                                                                                               <  1:1512/150‑1 (MQ=255)
           agatgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacc                                                                                                                                           <  2:965581/147‑2 (MQ=255)
           aGTTGTTTTTCTTGATGAGATGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacg                                                                                                                                           <  2:1034295/150‑1 (MQ=255)
           aGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacc                                                                                                                                           <  1:924439/150‑2 (MQ=255)
           aGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacg                                                                                                                                           >  1:370019/1‑150 (MQ=255)
            gttgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacgc                                                                                                                                          >  1:1759925/1‑150 (MQ=255)
            gttgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGGTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacgc                                                                                                                                          <  1:1415121/150‑1 (MQ=255)
            gttggtTTTCTTGCTGAGAAGCTGATGCAAACATCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacgc                                                                                                                                          <  2:1160866/150‑1 (MQ=255)
              tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccagg                                                                                                                                        <  1:774872/150‑5 (MQ=255)
              tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccagg                                                                                                                                        <  1:1437479/150‑5 (MQ=255)
               gttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCa                                                                                                                                       <  1:1504167/150‑1 (MQ=255)
                tttttCTTGATGAGAAGCTGATGCAAAAATCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCaa                                                                                                                                      <  2:1441279/150‑1 (MQ=255)
                  tttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAAc                                                                                                                                    <  2:534519/150‑1 (MQ=255)
                  tttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAAc                                                                                                                                    <  2:1407336/150‑1 (MQ=255)
                   ttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACa                                                                                                                                   <  1:1056075/150‑1 (MQ=255)
                   ttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACa                                                                                                                                   <  1:1609720/150‑1 (MQ=255)
                     cTTTATGAGAAGCTGATTCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATa                                                                                                                                 <  2:613890/150‑1 (MQ=255)
                      ttGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATaa                                                                                                                                >  2:1233/1‑150 (MQ=255)
                       tgatgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAAt                                                                                                                               >  1:767906/1‑150 (MQ=255)
                         atgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGa                                                                                                                             >  2:570613/1‑150 (MQ=255)
                           gagaAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGAtt                                                                                                                           >  2:100203/1‑150 (MQ=255)
                                    aTGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATaaaa                                                                                                                  >  1:1660029/1‑150 (MQ=255)
                                        aaaaTTCAGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTc                                                                                                              <  1:400067/150‑1 (MQ=255)
                                                  cTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGactac                                                                                                    <  1:609512/150‑1 (MQ=255)
                                                   tttATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGactact                                                                                                   >  1:1245260/1‑150 (MQ=255)
                                                       taATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGc                                                                                               >  2:936190/1‑150 (MQ=255)
                                                       taATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGc                                                                                               >  2:1166650/1‑150 (MQ=255)
                                                        aaTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGcc                                                                                              >  2:153876/1‑150 (MQ=255)
                                                        aaTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGcc                                                                                              <  1:1268643/150‑1 (MQ=255)
                                                         aTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGATACGCTCAAACATAATGATTCTAATAAACCCTCAGGAGACTACTATGccg                                                                                             >  2:1279718/1‑149 (MQ=255)
                                                          tGAAAATGATGCCAAAGCGAACGACCAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTg                                                                                            <  2:1793793/150‑1 (MQ=255)
                                                          tGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTg                                                                                            >  1:37898/1‑150 (MQ=255)
                                                             aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGCCTACTATGCCTGAAg                                                                                         >  2:131917/1‑150 (MQ=255)
                                                             aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAg                                                                                         <  1:645529/150‑1 (MQ=255)
                                                                tgatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCaa                                                                                      >  2:1811724/1‑150 (MQ=255)
                                                                  atgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACCTTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAaca                                                                                    <  1:181202/150‑1 (MQ=255)
                                                                    gCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCGTACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACAcc                                                                                  >  1:196563/1‑150 (MQ=255)
                                                                    gCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACAcc                                                                                  >  2:1549431/1‑150 (MQ=255)
                                                                      cAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCtt                                                                                >  1:1045271/1‑150 (MQ=255)
                                                                      cAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCtt                                                                                >  1:395836/1‑150 (MQ=255)
                                                                         aGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAATTGGGGTAACATTCACGCGACTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTTAAGCAACACCTTTTc                                                                             <  1:960358/150‑1 (MQ=255)
                                                                          gCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCa                                                                            <  1:1401208/150‑1 (MQ=255)
                                                                              aCGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtg                                                                        >  2:518044/1‑150 (MQ=255)
                                                                               cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                       >  2:21111/1‑150 (MQ=255)
                                                                               cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                       >  2:1555839/1‑150 (MQ=255)
                                                                               cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                       >  2:1364259/1‑150 (MQ=255)
                                                                                taCAATGTTGTAGTTTTCAATACATGTCCATACATAAAATTTTGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgat                                                                      <  1:1693635/149‑1 (MQ=255)
                                                                                gACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgat                                                                      <  1:1213281/150‑1 (MQ=255)
                                                                                gACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgat                                                                      <  1:1693631/150‑1 (MQ=255)
                                                                                gACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgat                                                                      <  1:588295/150‑1 (MQ=255)
                                                                                 aCAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgatg                                                                     <  2:681680/150‑1 (MQ=255)
                                                                                 aCAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgatg                                                                     <  2:1660029/150‑1 (MQ=255)
                                                                                    aGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTCCGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCAGAAGCAACACCTTTTCAGGTGATGAtt                                                                  >  1:1244750/1‑150 (MQ=255)
                                                                                     ggTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGAttt                                                                 >  2:1617379/1‑149 (MQ=255)
                                                                                      gTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTAAGGAGACTACTATGCCTGAAGCACCACCAATTCAGGTGATGATtgt                                                                >  1:352396/1‑150 (MQ=255)
                                                                                       ttGTAGTTTTCACTACATGTCCATACATAAAATGTGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATtgtg                                                               <  1:1448459/150‑1 (MQ=255)
                                                                                        tGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTgg                                                              >  2:386499/1‑150 (MQ=255)
                                                                                          tAGTTTTCACTACATGTCCATACATAAATTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgat                                                            >  1:1722032/1‑150 (MQ=255)
                                                                                           aGTTCTCACAACATGTCCAAACAAAAAATGGGGTAACATTCACGCGCCTGGCAGCGCTACCCACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatg                                                           <  2:1702219/150‑1 (MQ=255)
                                                                                               ttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGatca                                                       <  1:1166650/150‑1 (MQ=255)
                                                                                                 cACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGatcatc                                                     <  1:1034385/150‑1 (MQ=255)
                                                                                                 cACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGatcatc                                                     >  2:750578/1‑150 (MQ=255)
                                                                                                  aCTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATcc                                                    <  2:1106054/150‑1 (MQ=255)
                                                                                                      cATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACtt                                                <  2:1416325/150‑1 (MQ=255)
                                                                                                       aTGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTa                                               <  2:513797/150‑1 (MQ=255)
                                                                                                           ccATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCg                                           >  1:881524/1‑150 (MQ=255)
                                                                                                           ccATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCg                                           >  2:364794/1‑150 (MQ=255)
                                                                                                            catacataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGa                                          <  1:1132777/150‑1 (MQ=255)
                                                                                                                 ataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCgg                                     <  2:478251/150‑1 (MQ=255)
                                                                                                                  taAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGgt                                    <  2:657494/150‑1 (MQ=255)
                                                                                                                   aaaaTGGGTTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGgtg                                   <  2:1334855/150‑1 (MQ=255)
                                                                                                                    aaaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGgtgt                                  >  1:1021165/1‑150 (MQ=255)
                                                                                                                     aaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGtt                                 <  2:128796/150‑1 (MQ=255)
                                                                                                                     aaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGtt                                 <  2:767906/150‑1 (MQ=255)
                                                                                                                           gTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAg                           <  2:1026130/150‑1 (MQ=255)
                                                                                                                             aaCATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGtt                         >  1:775659/1‑150 (MQ=255)
                                                                                                                                aTTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACt                      >  1:1557755/1‑150 (MQ=255)
                                                                                                                                aTTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACt                      >  1:1138428/1‑150 (MQ=255)
                                                                                                                                 ttCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTg                     >  1:873184/1‑150 (MQ=255)
                                                                                                                                  tCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTgg                    >  1:1212970/1‑150 (MQ=255)
                                                                                                                                      tttgagcgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCt                >  2:516132/8‑150 (MQ=255)
                                                                                                                                        tgagcgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTg              >  1:520681/6‑150 (MQ=255)
                                                                                                                                           gcgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATc           >  2:522674/3‑150 (MQ=255)
                                                                                                                                            ggTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATcc          <  2:1238194/150‑1 (MQ=255)
                                                                                                                                            ggTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGAGCATCCACTTATGCGACGCGGGGTTCGTCAGTTACTGGAGCTTGATcc          <  2:1245260/150‑1 (MQ=255)
                                                                                                                                              gaGCGTTACCACCGCTACGCGCCAACATAATGATGCTAATAAACCCTCAGGCGCCGACTATGCCTGCAGCACCACCTTTTCAGGTGATGATTGTGGCTGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTg        <  2:702841/149‑1 (MQ=255)
                                                                                                                                               aGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGCCTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTgg       <  1:1018378/150‑1 (MQ=255)
                                                                                                                                               aGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTgg       <  1:1496740/150‑1 (MQ=255)
                                                                                                                                                gCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGc      <  1:100203/150‑1 (MQ=255)
                                                                                                                                                 cGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCCCTTATGCGACGCGGTGTTCGTCAGTTACTGGAGATTGATCATGGct     >  2:345384/1‑150 (MQ=255)
                                                                                                                                                 cGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGct     >  2:1032191/1‑150 (MQ=255)
                                                                                                                                                 cGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGct     >  1:608607/1‑150 (MQ=255)
                                                                                                                                                    tACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGCCGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTg  >  2:1066096/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTGATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTG  >  NC_000913/2290299‑2290596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: