Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,290,450 C→T 5.1% intergenic (‑270/‑50) yejO ← / → narP pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,290,4500CT 5.1% 417.4 / 7.0 137intergenic (‑270/‑50)yejO/narPpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ
Reads supporting (aligned to +/- strand):  ref base C (65/65);  new base T (3/4);  total (68/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

Only 100 of 142 total aligned reads displayed.

GATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAG  >  NC_000913/2290302‑2290599
                                                                                                                                                    |                                                                                                                                                     
gattTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCa                                                                                                                                                      >  1:513797/1‑150 (MQ=255)
  ttttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAac                                                                                                                                                    >  1:1528487/1‑150 (MQ=255)
  ttttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAac                                                                                                                                                    >  2:1693631/1‑150 (MQ=255)
  ttttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAac                                                                                                                                                    >  1:725724/1‑150 (MQ=255)
  ttttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCAATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAac                                                                                                                                                    >  2:1693635/1‑150 (MQ=255)
   tttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacg                                                                                                                                                   >  1:1341727/1‑150 (MQ=255)
   tttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacg                                                                                                                                                   <  1:180034/150‑1 (MQ=255)
    ttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCGATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgc                                                                                                                                                  <  1:1512/150‑1 (MQ=255)
       tAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTATTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctac                                                                                                                                               <  2:702333/150‑1 (MQ=255)
        agatgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacc                                                                                                                                              <  2:965581/147‑2 (MQ=255)
        aGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacc                                                                                                                                              <  1:924439/150‑2 (MQ=255)
        aGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacg                                                                                                                                              >  1:370019/1‑150 (MQ=255)
         gttgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacgc                                                                                                                                             >  1:1759925/1‑150 (MQ=255)
         gttgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGGTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacgc                                                                                                                                             <  1:1415121/150‑1 (MQ=255)
         gttggtTTTCTTGCTGAGAAGCTGATGCAAACATCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacgc                                                                                                                                             <  2:1160866/150‑1 (MQ=255)
           tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccagg                                                                                                                                           <  1:774872/150‑5 (MQ=255)
             tttttCTTGATGAGAAGCTGATGCAAAAATCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCaa                                                                                                                                         <  2:1441279/150‑1 (MQ=255)
              ttttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCaaa                                                                                                                                        >  2:1146720/1‑150 (MQ=255)
              ttttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCaaa                                                                                                                                        <  2:1297439/150‑1 (MQ=255)
               tttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAAc                                                                                                                                       <  2:534519/150‑1 (MQ=255)
               tttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAAc                                                                                                                                       <  2:1407336/150‑1 (MQ=255)
                ttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACa                                                                                                                                      <  1:1609720/150‑1 (MQ=255)
                  cTTTATGAGAAGCTGATTCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATa                                                                                                                                    <  2:613890/150‑1 (MQ=255)
                   ttGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATaa                                                                                                                                   >  2:1233/1‑150 (MQ=255)
                    tgatgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAAt                                                                                                                                  >  1:767906/1‑150 (MQ=255)
                      atgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGa                                                                                                                                >  2:570613/1‑150 (MQ=255)
                                     aaaaTTCCGTCTTTTTAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTc                                                                                                                 <  1:393342/150‑1 (MQ=255)
                                     aaaaTTCAGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTc                                                                                                                 <  1:400067/150‑1 (MQ=255)
                                       aaTTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAg                                                                                                               <  1:1408912/150‑1 (MQ=255)
                                            cGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGgagaa                                                                                                          >  1:1695260/1‑149 (MQ=255)
                                               cTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGactac                                                                                                       <  1:609512/150‑1 (MQ=255)
                                                tttATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCGATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGactact                                                                                                      >  1:1238194/1‑150 (MQ=255)
                                                    taATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGc                                                                                                  >  2:1166650/1‑150 (MQ=255)
                                                    taATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGc                                                                                                  >  2:936190/1‑150 (MQ=255)
                                                     aaTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGcc                                                                                                 <  1:1268643/150‑1 (MQ=255)
                                                     aaTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGcc                                                                                                 >  2:153876/1‑150 (MQ=255)
                                                       tGAAAATGATGCCAAAGCGAACGACCAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTg                                                                                               <  2:1793793/150‑1 (MQ=255)
                                                       tGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTg                                                                                               >  1:37898/1‑150 (MQ=255)
                                                          aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGCCTACTATGCCTGAAg                                                                                            >  2:131917/1‑150 (MQ=255)
                                                          aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAg                                                                                            <  1:645529/150‑1 (MQ=255)
                                                                 gCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCGTACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACAcc                                                                                     >  1:196563/1‑150 (MQ=255)
                                                                 gCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACAcc                                                                                     <  1:1268887/150‑1 (MQ=255)
                                                                 gCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACAcc                                                                                     >  2:1549431/1‑150 (MQ=255)
                                                                   cAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCtt                                                                                   >  2:1048256/1‑150 (MQ=255)
                                                                   cAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCtt                                                                                   >  1:1045271/1‑150 (MQ=255)
                                                                      aGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAATTGGGGTAACATTCACGCGACTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTTAAGCAACACCTTTTc                                                                                <  1:960358/150‑1 (MQ=255)
                                                                           aCGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtg                                                                           >  2:518044/1‑150 (MQ=255)
                                                                            cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                          >  2:1364259/1‑150 (MQ=255)
                                                                            cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                          >  2:1555839/1‑150 (MQ=255)
                                                                            cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                          >  2:21111/1‑150 (MQ=255)
                                                                            cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACAATGCCTGAAGCAACACCTTTTCAGGtga                                                                          >  2:787740/1‑150 (MQ=255)
                                                                             taCAATGTTGTAGTTTTCAATACATGTCCATACATAAAATTTTGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgat                                                                         <  1:1693635/149‑1 (MQ=255)
                                                                             gACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgat                                                                         <  1:1693631/150‑1 (MQ=255)
                                                                              aCAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgatg                                                                        <  2:681680/150‑1 (MQ=255)
                                                                              aCAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgatg                                                                        <  2:1660029/150‑1 (MQ=255)
                                                                                 aGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTCCGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCAGAAGCAACACCTTTTCAGGTGATGAtt                                                                     >  1:1244750/1‑150 (MQ=255)
                                                                                    ttGTAGTTTTCACTACATGTCCATACATAAAATGTGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATtgtg                                                                  <  1:1448459/150‑1 (MQ=255)
                                                                                    ttGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATtgtg                                                                  <  2:644485/150‑1 (MQ=255)
                                                                                    ttGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATtgtg                                                                  <  2:1711217/150‑1 (MQ=255)
                                                                                     tGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTgg                                                                 >  2:386499/1‑150 (MQ=255)
                                                                                         gTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCATTTCAGGTGATGATTGTGgatga                                                             >  2:101987/1‑150 (MQ=255)
                                                                                            ttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGatca                                                          <  1:1166650/150‑1 (MQ=255)
                                                                                              cACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGatcatc                                                        <  1:1034385/150‑1 (MQ=255)
                                                                                                   cATGTGAATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATTCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACtt                                                   <  1:411652/150‑1 (MQ=255)
                                                                                                   cATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACtt                                                   <  2:1416325/150‑1 (MQ=255)
                                                                                                    aTGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTa                                                  <  2:513797/150‑1 (MQ=255)
                                                                                                     tGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTAt                                                 >  1:1614698/1‑150 (MQ=255)
                                                                                                        ccATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCg                                              >  1:881524/1‑150 (MQ=255)
                                                                                                        ccATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCg                                              >  2:364794/1‑150 (MQ=255)
                                                                                                         catacataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGa                                             <  1:1132777/150‑1 (MQ=255)
                                                                                                            acataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGAcgc                                          <  1:1450338/150‑1 (MQ=255)
                                                                                                             cataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGAcgcg                                         >  1:539653/1‑150 (MQ=255)
                                                                                                              ataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCgg                                        <  2:478251/150‑1 (MQ=255)
                                                                                                                 aaaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGgtgt                                     >  1:1021165/1‑150 (MQ=255)
                                                                                                                  aaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGtt                                    <  2:128796/150‑1 (MQ=255)
                                                                                                                  aaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGtt                                    <  2:767906/150‑1 (MQ=255)
                                                                                                                   aTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGgtgtgc                                   >  1:261220/1‑148 (MQ=255)
                                                                                                                        gTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAg                              <  2:1026130/150‑1 (MQ=255)
                                                                                                                          aaCATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGtt                            >  1:775659/1‑150 (MQ=255)
                                                                                                                            cATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTAc                          >  1:1092653/1‑150 (MQ=255)
                                                                                                                             aTTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACt                         >  1:1138428/1‑150 (MQ=255)
                                                                                                                              ttCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTg                        >  1:873184/1‑150 (MQ=255)
                                                                                                                               tCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTgg                       >  1:1212970/1‑150 (MQ=255)
                                                                                                                                cACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACATATGCGACGCGGTGTTCGTCAGATACTGGa                      >  2:1224727/1‑150 (MQ=255)
                                                                                                                                   tttgagcgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCt                   >  2:516132/8‑150 (MQ=255)
                                                                                                                                     tgagcgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTg                 >  1:520681/6‑150 (MQ=255)
                                                                                                                                      ccTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGa                >  2:1277992/1‑150 (MQ=255)
                                                                                                                                        gcgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATc              >  2:522674/3‑150 (MQ=255)
                                                                                                                                         ggTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGAGCATCCACTTATGCGACGCGGGGTTCGTCAGTTACTGGAGCTTGATcc             <  2:1245260/150‑1 (MQ=255)
                                                                                                                                           gaGCGTTACCACCGCTACGCGCCAACATAATGATGCTAATAAACCCTCAGGCGCCGACTATGCCTGCAGCACCACCTTTTCAGGTGATGATTGTGGCTGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTg           <  2:702841/149‑1 (MQ=255)
                                                                                                                                            aGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGCCTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTgg          <  1:1018378/150‑1 (MQ=255)
                                                                                                                                            aGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTgg          <  1:1496740/150‑1 (MQ=255)
                                                                                                                                             gCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGc         <  1:100203/150‑1 (MQ=255)
                                                                                                                                              cGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCCCTTATGCGACGCGGTGTTCGTCAGTTACTGGAGATTGATCATGGct        >  2:345384/1‑150 (MQ=255)
                                                                                                                                              cGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGct        >  2:1032191/1‑150 (MQ=255)
                                                                                                                                              cGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGct        >  1:608607/1‑150 (MQ=255)
                                                                                                                                                 tACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGCCGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTg     >  2:1066096/1‑150 (MQ=255)
                                                                                                                                                   ccAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGaa   >  2:1561322/1‑150 (MQ=255)
                                                                                                                                                   ccAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGaa   <  2:1341727/150‑1 (MQ=255)
                                                                                                                                                   ccAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGaa   >  2:194524/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAG  >  NC_000913/2290302‑2290599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: