Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 324,585 G→A 5.1% intergenic (+132/+111) ykgG → / ← ykgH LutC family protein; putative electron transport chain YkgEFG component/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913324,5850GA 5.1% 421.3 / 6.6 136intergenic (+132/+111)ykgG/ykgHLutC family protein; putative electron transport chain YkgEFG component/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (81/47);  new base A (2/5);  total (84/52)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

TTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCAT  >  NC_000913/324437‑324731
                                                                                                                                                    |                                                                                                                                                  
 tattGAGGATTGTTGAGAGATATATGAAGTCTGGACATAAACCTGAAGAAGTCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATTCTGCTCTTCTGTAAATATCCTGGTATTACCAATGcc                                                                                                                                                  <  2:714107/150‑1 (MQ=255)
      aGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGat                                                                                                                                             >  2:1303281/1‑150 (MQ=255)
       ggATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGata                                                                                                                                            <  1:1720055/150‑1 (MQ=255)
       ggATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAAAGCCAGGata                                                                                                                                            >  1:619497/1‑150 (MQ=255)
            tttgAGAGAGATATGAAGTCTTGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTAc                                                                                                                                       <  1:577014/149‑1 (MQ=255)
            gttgAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTATGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTTCTATTCTGTAAATATCCTGGTATTAACAATTCCATGATATTTAc                                                                                                                                       >  1:1147871/1‑150 (MQ=255)
            gttgAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTAc                                                                                                                                       >  2:1088333/1‑150 (MQ=255)
            gttgAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTAc                                                                                                                                       <  1:569675/150‑1 (MQ=255)
            gttgAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTAc                                                                                                                                       >  2:69561/1‑150 (MQ=255)
            gttgAGAGAGAAATGAAGTCTGGACAAAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGCATTGGTAATACCAGGATATTTAc                                                                                                                                       <  1:1168617/150‑1 (MQ=38)
              tgAGAGAGATATGAAGTCTGGACATAAACATGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAg                                                                                                                                     >  1:165660/1‑150 (MQ=255)
               gagagataTATGAAGTCTGGACATAAACCAGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGt                                                                                                                                    <  1:299501/150‑1 (MQ=255)
                cgagagaTATGAAGGCTGGACATAAACCTGACGACGGCATACGAGGGTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTg                                                                                                                                   <  1:758258/149‑1 (MQ=255)
                 gagagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGa                                                                                                                                  >  2:362683/1‑150 (MQ=255)
                   gagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaa                                                                                                                                <  1:954229/150‑1 (MQ=255)
                   gagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaa                                                                                                                                <  2:745935/150‑1 (MQ=255)
                    agaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaaa                                                                                                                               >  1:1434105/1‑150 (MQ=255)
                    agaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaaa                                                                                                                               >  1:343423/1‑150 (MQ=255)
                     gaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGATGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaaaa                                                                                                                              >  1:1307929/1‑150 (MQ=255)
                     gaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTACGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaaaa                                                                                                                              >  1:782166/1‑150 (MQ=255)
                        atGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGAc                                                                                                                           >  1:700236/1‑150 (MQ=255)
                         tGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACa                                                                                                                          >  2:525953/1‑150 (MQ=255)
                         tGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACa                                                                                                                          >  1:349637/1‑150 (MQ=255)
                             gTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCa                                                                                                                      <  1:35688/150‑1 (MQ=255)
                                tGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATcc                                                                                                                   >  2:1701356/1‑150 (MQ=255)
                                 ggACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCa                                                                                                                  >  2:255960/1‑150 (MQ=255)
                                   aCATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGATTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCAtt                                                                                                                >  2:1591346/1‑150 (MQ=255)
                                     aTAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGTCATCCATTcc                                                                                                              >  1:1215939/1‑150 (MQ=255)
                                      tAAACCGGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTACCCCCGTTCCGTCATGGTTATGCTGCTCTGCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCt                                                                                                             <  1:883736/150‑1 (MQ=255)
                                       aaaCCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTg                                                                                                            >  2:833213/1‑150 (MQ=255)
                                        aaacttaagaagGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGa                                                                                                           <  1:894657/144‑1 (MQ=255)
                                        aaCCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATAATTACAGTGAAAAAGACAGGCATCCATTCCTGa                                                                                                           >  1:799554/1‑150 (MQ=255)
                                         aCCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaa                                                                                                          >  1:374198/1‑150 (MQ=255)
                                               agaagGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACggg                                                                                                    <  2:926946/150‑1 (MQ=255)
                                               agaagGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACggg                                                                                                    >  2:409395/1‑150 (MQ=255)
                                                gaatGCATACGAGGTTGAAAATAGAGTTTAGGACAAACTGACTTAACACATTTCCGTAATGTTTATGATGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCTTTCCTGAAAACGGGt                                                                                                   <  1:167503/150‑1 (MQ=255)
                                                gaagGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGt                                                                                                   >  2:1806367/1‑150 (MQ=255)
                                                 atggCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAATACGGGtt                                                                                                  <  1:1082453/148‑1 (MQ=255)
                                                 aagGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGtt                                                                                                  <  2:1564359/150‑1 (MQ=255)
                                                  agGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttc                                                                                                 <  1:586010/150‑1 (MQ=255)
                                                  agGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCATCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttc                                                                                                 <  2:1315522/150‑1 (MQ=255)
                                                     cATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttccct                                                                                              >  2:68340/1‑150 (MQ=255)
                                                     cATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTCTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttccct                                                                                              >  1:470021/1‑150 (MQ=255)
                                                      aTACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttccctt                                                                                             >  2:149038/1‑150 (MQ=255)
                                                       aaCGAGGTTGAAAATAGCGTTTAGGACAACCAGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttccctac                                                                                            >  1:484864/2‑148 (MQ=255)
                                                        aCGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttcccttcc                                                                                           >  1:1076028/1‑150 (MQ=255)
                                                             gTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTc                                                                                      <  2:812387/150‑1 (MQ=255)
                                                              ttGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCa                                                                                     <  1:163780/150‑1 (MQ=255)
                                                              ttGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCa                                                                                     <  2:1809236/150‑1 (MQ=255)
                                                               tGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTATGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCAt                                                                                    <  1:1780152/150‑1 (MQ=255)
                                                                gAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCAtt                                                                                   <  1:1225510/150‑1 (MQ=255)
                                                                        cGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAATAAGACAGGCATCCATGCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTc                                                                           >  1:1102481/1‑150 (MQ=255)
                                                                         gTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCGCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCa                                                                          <  1:88919/150‑1 (MQ=255)
                                                                             aGGACTACCTGACTTAACCCCTTTCCTTCATGGTTATGCTGCTCTTCTGTAAATATCATTGTATTACGAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCATGAAAACGGGTTCCCTTCCCGTTCATTGTCACTTCAGCtt                                                                      <  1:1328618/150‑1 (MQ=255)
                                                                              ggACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCttt                                                                     >  1:1699398/1‑150 (MQ=255)
                                                                               gACAACCTTACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATACTTGTATTACCAATGACAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTta                                                                    <  1:181644/150‑1 (MQ=255)
                                                                               gACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTACCGGGCATTGTCACTTCAGCTTta                                                                    <  2:1611360/150‑1 (MQ=255)
                                                                                   aCCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCACTTCCCGGTCATTGTCTCTTCAGATTTATACa                                                                >  1:1141718/1‑150 (MQ=255)
                                                                                       gACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCa                                                            >  2:1031273/1‑150 (MQ=255)
                                                                                         cTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                          >  2:411606/1‑150 (MQ=255)
                                                                                         cTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                          >  1:1196978/1‑150 (MQ=255)
                                                                                          ttAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCActc                                                         >  1:481072/1‑150 (MQ=255)
                                                                                          ttAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCActc                                                         >  2:1732528/1‑150 (MQ=255)
                                                                                           tAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATCCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCActct                                                        >  1:670061/1‑150 (MQ=255)
                                                                                             aCCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCActctct                                                      >  2:1375459/1‑150 (MQ=255)
                                                                                              ccccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTa                                                     <  2:349500/150‑1 (MQ=255)
                                                                                              ccccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTa                                                     >  2:1598512/1‑150 (MQ=255)
                                                                                                ccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATc                                                   >  2:104268/1‑150 (MQ=255)
                                                                                                  gTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAg                                                 >  2:1552421/1‑150 (MQ=255)
                                                                                                    tCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGaa                                               <  1:1580458/150‑1 (MQ=255)
                                                                                                     ccGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCGAGGATATTTACAGTGGAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCCCTTCAGCTTTATACACGCCCTCTATAGCAGAAt                                              >  1:1146378/1‑150 (MQ=255)
                                                                                                      cGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtg                                             >  1:412273/1‑150 (MQ=255)
                                                                                                       gTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtgt                                            >  2:1061090/1‑150 (MQ=255)
                                                                                                         cATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCAGGTCTTTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtgttt                                          >  1:1391144/1‑149 (MQ=255)
                                                                                                          aTGGTTATGCTGCTCTTCTGTAAATATCCTGGTATAACCAATGCCAGGATATTTAGAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtgttgt                                         <  1:1255207/150‑1 (MQ=255)
                                                                                                           tGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATgttgtt                                        >  1:977370/1‑150 (MQ=255)
                                                                                                           tGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATgttgtt                                        >  2:1156328/1‑150 (MQ=255)
                                                                                                                  gctgctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACt                                 <  1:1193507/150‑1 (MQ=255)
                                                                                                                   ctgctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCTTTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTg                                >  1:914409/1‑150 (MQ=255)
                                                                                                                   ctgctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCATCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCATAATGTTGTTTATTACTg                                >  2:1138084/1‑150 (MQ=255)
                                                                                                                           ctGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGt                        <  2:374198/150‑1 (MQ=255)
                                                                                                                           ctGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGt                        <  2:516755/150‑1 (MQ=255)
                                                                                                                            tGTAAATATCCTGGCATTGGTAATACGAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGtt                       >  1:279854/1‑150 (MQ=39)
                                                                                                                             gTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGttt                      <  1:833213/150‑1 (MQ=255)
                                                                                                                             gTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGttt                      <  2:165660/150‑1 (MQ=255)
                                                                                                                              tAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGtttt                     <  1:1591346/150‑1 (MQ=255)
                                                                                                                                aaTATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttgt                   <  2:619497/150‑1 (MQ=255)
                                                                                                                                 atatCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttgtt                  >  2:1177724/1‑150 (MQ=255)
                                                                                                                                  tatCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGAACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttgttg                 >  2:1204617/1‑150 (MQ=255)
                                                                                                                                    tCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCTTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGt               >  2:1226305/1‑150 (MQ=255)
                                                                                                                                     ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTa              >  2:1703271/1‑150 (MQ=255)
                                                                                                                                      cTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCCGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTAt             >  2:1051593/1‑150 (MQ=255)
                                                                                                                                      cTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTAt             >  2:1165092/1‑150 (MQ=255)
                                                                                                                                        ggTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTc           <  2:700236/150‑1 (MQ=255)
                                                                                                                                        ggTATTACCAAAGCCAGGATATATACAGTGAAAAAGACAGGCATCCATTCCTGCAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTc           >  1:1233449/1‑150 (MQ=255)
                                                                                                                                         gTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCa          <  1:1580919/150‑1 (MQ=255)
                                                                                                                                         gTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGATTTGTTGGTATTCa          <  1:409395/150‑1 (MQ=255)
                                                                                                                                             tACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTa      >  2:1703163/1‑150 (MQ=255)
                                                                                                                                               ccAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTAcc    >  1:677101/1‑150 (MQ=255)
                                                                                                                                                 aaTGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCAt  <  1:362683/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCAT  >  NC_000913/324437‑324731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: