Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,364,998 C→A 9.4% intergenic (+19/+20) nudI → / ← ais nucleoside triphosphatase/putative LPS core heptose(II)‑phosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,364,9980CA 9.4% 347.8 / 20.7 123intergenic (+19/+20)nudI/aisnucleoside triphosphatase/putative LPS core heptose(II)‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (55/56);  new base A (5/7);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 123 total aligned reads displayed.

CTGCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAAT  >  NC_000913/2364852‑2365142
                                                                                                                                                  |                                                                                                                                                
cTGCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTGCGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTgg                                                                                                                                               >  2:188012/1‑150 (MQ=255)
 tGCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGc                                                                                                                                              <  1:829330/150‑1 (MQ=255)
  gCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCATGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCt                                                                                                                                             <  2:1272167/150‑1 (MQ=255)
       ccGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCat                                                                                                                                        <  1:1090044/150‑2 (MQ=255)
       ccGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCat                                                                                                                                        <  1:1803460/150‑2 (MQ=255)
        cGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCatc                                                                                                                                       <  1:1608750/150‑3 (MQ=255)
        cGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATCACAGCCAGTAATGGCTGTCatc                                                                                                                                       <  1:1492785/150‑3 (MQ=255)
        cGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATCACAGCCAGTAATGGCTGTCatc                                                                                                                                       <  1:1197900/150‑3 (MQ=255)
           gaAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTg                                                                                                                                    >  1:1646214/1‑150 (MQ=255)
           gaAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTg                                                                                                                                    >  2:1266082/1‑150 (MQ=255)
             aGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaa                                                                                                                                  >  1:1218772/1‑150 (MQ=255)
              gTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaaa                                                                                                                                 >  1:1820284/1‑150 (MQ=255)
               tGAAAATAAACGAAGCGTTTCAGGACTACGCTTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCTACACCCGAAAAACGTTACTTTTGAAAGGTCTTCTGTAACAATGATGACAGACATTACTGTATGTCAGATTTaaaa                                                                                                                                >  2:1423883/1‑150 (MQ=255)
                gAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAAt                                                                                                                               >  1:1323156/1‑150 (MQ=255)
                   aaTAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTa                                                                                                                            <  2:781728/150‑1 (MQ=255)
                     tAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGt                                                                                                                          <  2:1623001/150‑1 (MQ=255)
                       aaCGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTAATGGCTGTCAGATTGAAAATTTAGTgg                                                                                                                        >  2:1079673/1‑150 (MQ=255)
                        aCGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGt                                                                                                                       >  1:723713/1‑150 (MQ=255)
                         cGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGtt                                                                                                                      >  1:252907/1‑150 (MQ=255)
                          gAATAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTTCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTa                                                                                                                     <  1:1034561/150‑1 (MQ=255)
                               gTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGaa                                                                                                                >  2:1660997/1‑150 (MQ=255)
                               gTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGaa                                                                                                                >  2:750875/1‑150 (MQ=255)
                                tttCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAAt                                                                                                               <  1:1030994/150‑1 (MQ=255)
                                 ttCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAAtt                                                                                                              >  1:1665512/1‑150 (MQ=255)
                                  tCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTc                                                                                                             >  1:773087/1‑150 (MQ=255)
                                   cAGGACTACGCGTGGGTAAAACCTGAATATCTAGAGCATTATTATTTGAATGTCGACACCCGAAAAACGTTACGTTTGAAAGGTCTTCTCTAACAATGATGACAGCCATTACTGGATGTCAGATTGAAAATTTAGTGGTTAACTAATTca                                                                                                            >  1:839316/1‑149 (MQ=255)
                                   cAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTcc                                                                                                            <  1:1485552/150‑1 (MQ=255)
                                    aGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATATGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACACCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTccc                                                                                                           <  1:1740689/150‑1 (MQ=255)
                                     ggACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTcccc                                                                                                          >  1:1531618/1‑150 (MQ=255)
                                       aCTACGCGTTGGTAAAACCTCAAGATTTGGTGCATTATGATTTGAATTTCGCCACCCGAAAAACGTTACGTTTGAAATGTCTTATGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCAt                                                                                                        <  1:1132044/150‑1 (MQ=255)
                                        gtACGCGTGGGTAAAACCTGAAGATCTGGTGCATTAAGATTAGAATCTCGCCACCCGAAAAACGATACGTTTGAAAGGTCTTCTGAAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATc                                                                                                       >  1:338523/2‑150 (MQ=255)
                                        cTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATc                                                                                                       >  2:1591029/1‑150 (MQ=255)
                                         tACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATcc                                                                                                      <  1:1809840/150‑1 (MQ=255)
                                          aCGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCa                                                                                                     <  2:313963/150‑1 (MQ=255)
                                           cgcgTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAg                                                                                                    <  1:1722631/150‑1 (MQ=255)
                                            tagtgtgtAAAACATGAAGATCTGGTGAATTATGATTTGAATGTCGCAACCCGAAAAACGTTACTTTTTAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGa                                                                                                   <  1:324860/148‑1 (MQ=255)
                                             cgTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGAt                                                                                                  >  2:1604922/1‑150 (MQ=255)
                                             cgTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGAt                                                                                                  >  1:1177569/1‑150 (MQ=255)
                                              gggggTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATa                                                                                                 <  2:1619782/148‑1 (MQ=255)
                                              gTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATa                                                                                                 >  2:949880/1‑150 (MQ=255)
                                                   tAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACtt                                                                                            >  1:1368900/1‑150 (MQ=255)
                                                       aCCTGAAGATCTGGTGCATTATGATTTGAATGTCCCCACCCGAAAAACGTTACGTATGAAAGGTCTTCTCTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAATTTTAGTGTTTAAAGAATTCCCCATCCAGATAAACTTTGcc                                                                                        >  2:483598/1‑150 (MQ=255)
                                                       aCCTGAAGATCTGGTGCATTATGATATGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACACTGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGcc                                                                                        <  2:196739/150‑1 (MQ=255)
                                                        ccTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCt                                                                                       >  1:511712/1‑150 (MQ=255)
                                                        ccTGAAGATCTGGTGCATGATGATTTGAATGTCGCCACCAGAAAAACGTTACGTTTGAAATGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCACCATCCAGATAAACTTTTCCt                                                                                       <  2:1168798/150‑1 (MQ=255)
                                                          tGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCttt                                                                                     >  2:673799/1‑150 (MQ=255)
                                                           gAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCtttt                                                                                    >  1:1017304/1‑150 (MQ=255)
                                                             aGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGTCTTTTTc                                                                                  <  1:1068449/150‑1 (MQ=255)
                                                             aGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTc                                                                                  >  1:1186713/1‑150 (MQ=255)
                                                              gATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTcc                                                                                 <  1:1178937/150‑1 (MQ=255)
                                                               aTCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCca                                                                                <  1:1708659/150‑1 (MQ=255)
                                                                tCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCcac                                                                               <  2:1218772/150‑1 (MQ=255)
                                                                 cTGGTGCATTATGATTTGAATGGCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCcaca                                                                              <  2:1383416/150‑1 (MQ=255)
                                                                  tGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcat                                                                             >  1:739356/1‑150 (MQ=255)
                                                                   ggTGCATTATGATTTGCATGTCGCCACCCGAAAAACGGTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatg                                                                            <  2:1721421/150‑1 (MQ=255)
                                                                   ggTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatg                                                                            <  2:1265653/150‑1 (MQ=255)
                                                                    gTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTGCGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatgc                                                                           <  1:188012/150‑1 (MQ=255)
                                                                      gCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAACCTTTGCCTTTTTCCAcatgcat                                                                         >  2:1123128/1‑150 (MQ=255)
                                                                       cATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatgcatg                                                                        <  1:52/150‑1 (MQ=255)
                                                                       cATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCACATAAACTTTGCCTTTTTCCAcatgcatg                                                                        <  1:1270087/150‑1 (MQ=255)
                                                                        aTTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATTCATGa                                                                       >  2:45054/1‑150 (MQ=255)
                                                                          tATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACt                                                                     >  2:966617/1‑150 (MQ=255)
                                                                             gATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTaac                                                                  >  2:733802/1‑149 (MQ=255)
                                                                              aTTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAAc                                                                 >  2:963468/1‑150 (MQ=255)
                                                                               tttGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGCCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAAcc                                                                >  2:1381368/1‑150 (MQ=255)
                                                                                  gAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATc                                                             <  2:1518983/150‑1 (MQ=255)
                                                                                   aaTGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATcc                                                            <  2:606004/150‑1 (MQ=255)
                                                                                       tCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGAt                                                        >  1:28459/1‑150 (MQ=255)
                                                                                        cGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATa                                                       <  2:1784551/150‑1 (MQ=255)
                                                                                          cctcccGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTATCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAAt                                                     <  2:617903/147‑1 (MQ=255)
                                                                                            aCCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCa                                                   <  1:991421/150‑1 (MQ=255)
                                                                                             cccGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAg                                                  >  1:140595/1‑150 (MQ=255)
                                                                                              ccGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAgg                                                 >  2:345234/1‑150 (MQ=255)
                                                                                                   aaaCGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTaa                                            <  1:242246/150‑1 (MQ=255)
                                                                                                    aaagttacgttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCTTGACTAAACCATCCAGATAATCAGGTTTaaa                                           <  1:46148/147‑1 (MQ=255)
                                                                                                       gttacgttTGAAAGGTCTTCTGTATCAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTTCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAAAGt                                        >  2:549250/1‑150 (MQ=255)
                                                                                                           cgttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTcgcg                                    >  2:367849/1‑150 (MQ=255)
                                                                                                            gttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGt                                   >  1:484415/1‑150 (MQ=255)
                                                                                                             tttGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTc                                  >  2:1448987/1‑150 (MQ=255)
                                                                                                             tttGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTc                                  >  2:445140/1‑150 (MQ=255)
                                                                                                              ttGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCa                                 >  2:1133896/1‑150 (MQ=255)
                                                                                                                taaaGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACg                               <  1:204631/149‑1 (MQ=255)
                                                                                                                      tcttctGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATc                         <  2:1017304/150‑1 (MQ=255)
                                                                                                                          ctGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTa                     >  2:796155/1‑150 (MQ=255)
                                                                                                                             tAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCa                  <  1:1591029/150‑1 (MQ=255)
                                                                                                                             tAACAATGATGACAGCCATTACTGGATGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACATTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCa                  <  1:318392/150‑1 (MQ=255)
                                                                                                                              aaCAATGATGACAGCCATTACTGGCTTTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCaa                 <  2:488018/150‑1 (MQ=255)
                                                                                                                              aaCAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCaa                 <  2:1546726/150‑1 (MQ=255)
                                                                                                                              aaCAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAAACATCCAGATAATCAGGTTTAATTGTCGCGTCAAGCTTATATTGAGaaa                 >  2:1143287/1‑150 (MQ=255)
                                                                                                                               ttcaatctgaCAGCCAGTAATGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAat                >  2:1640130/8‑150 (MQ=255)
                                                                                                                               aCAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAat                >  1:1203357/1‑150 (MQ=255)
                                                                                                                                cAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAata               >  1:731833/1‑150 (MQ=255)
                                                                                                                                 aaTGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAatat              <  2:140595/150‑1 (MQ=255)
                                                                                                                                  atgatgACAGCCATTACTGGCTGTCAGATTTAAAATTTAGTGGTTAACGAAATCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCAACCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAatata             <  1:308436/150‑1 (MQ=255)
                                                                                                                                  aatctgaCAGCCAGTAATGGCGGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAagata             >  1:1708626/5‑150 (MQ=255)
                                                                                                                                     ctgaCAGCCAGTAATGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGACTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGt          >  1:1622468/2‑150 (MQ=255)
                                                                                                                                      tgaCAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTc         <  2:723713/150‑1 (MQ=255)
                                                                                                                                       taCAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCa        <  2:918442/149‑1 (MQ=255)
                                                                                                                                          aGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAAATCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGc     <  2:810381/150‑1 (MQ=255)
                                                                                                                                            ccATTACTGGCAGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCaa   >  2:488071/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CTGCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAAT  >  NC_000913/2364852‑2365142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: