Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,399,828 T→G 5.4% H113P (CAT→CCT)  nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,399,8280TG 5.4% 316.6 / 6.3 107H113P (CAT→CCT) nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base T (48/52);  new base G (4/2);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 109 total aligned reads displayed.

TGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACC  >  NC_000913/2399682‑2399977
                                                                                                                                                  |                                                                                                                                                     
tGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGc                                                                                                                                                    <  2:1804623/150‑1 (MQ=255)
 gtagtaACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCa                                                                                                                                                   >  2:631797/1‑150 (MQ=255)
  tagtaACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAg                                                                                                                                                  <  1:961296/150‑1 (MQ=255)
      aaCGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTAc                                                                                                                                              >  1:1606225/1‑150 (MQ=255)
      aaCGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTAc                                                                                                                                              >  1:1579479/1‑150 (MQ=255)
        cGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCg                                                                                                                                            <  1:619404/150‑1 (MQ=255)
        cGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCg                                                                                                                                            <  2:1011312/150‑1 (MQ=255)
            cacaGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGAAGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCct                                                                                                                                        >  2:1085535/1‑150 (MQ=255)
                gCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACTGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATACTGAAGATGGCAGTTACCGCCCTCTTc                                                                                                                                    <  2:848343/150‑1 (MQ=255)
                gCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTc                                                                                                                                    <  2:838670/150‑1 (MQ=255)
                gCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTc                                                                                                                                    <  2:517670/150‑1 (MQ=255)
                gCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTc                                                                                                                                    <  2:1012317/150‑1 (MQ=255)
                gCGGAAGCAGGCGATGCAGCGGTGCATTTCGTGAGCGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGGCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTc                                                                                                                                    <  2:1622052/150‑1 (MQ=255)
                 cGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAACTCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACAGCCCTCTTca                                                                                                                                   >  1:1334382/1‑150 (MQ=255)
                 cGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTca                                                                                                                                   <  1:1005581/150‑1 (MQ=255)
                  ggTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTcac                                                                                                                                  <  1:1764644/150‑1 (MQ=255)
                  ggTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGGGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTcac                                                                                                                                  <  2:410094/150‑1 (MQ=255)
                   gTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGAGGGCGGTAACTGCCATCTTcagg                                                                                                                                 <  2:1787747/150‑3 (MQ=255)
                        aGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCg                                                                                                                            >  1:1626566/1‑150 (MQ=255)
                        aGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCg                                                                                                                            >  2:1609402/1‑150 (MQ=255)
                         ggCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCgg                                                                                                                           >  1:1355939/1‑150 (MQ=255)
                         ggCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCgg                                                                                                                           <  2:332917/150‑1 (MQ=255)
                          gCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGa                                                                                                                          >  2:485051/1‑150 (MQ=255)
                           cGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGAc                                                                                                                         >  2:296762/1‑150 (MQ=255)
                              tGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGt                                                                                                                      <  2:523925/150‑1 (MQ=255)
                              tGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGt                                                                                                                      >  1:758090/1‑150 (MQ=255)
                                 aGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGATGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGt                                                                                                                   >  1:347769/1‑150 (MQ=255)
                                        cATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACATTCGTGCGggtg                                                                                                            >  1:1595911/1‑150 (MQ=255)
                                        cATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGggtg                                                                                                            <  1:1046312/150‑1 (MQ=255)
                                          tttCGAGAGAGATGACTGGCACCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGggtggt                                                                                                          <  2:8326/150‑1 (MQ=255)
                                           ttCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGtggtt                                                                                                         >  2:1085317/1‑150 (MQ=255)
                                              gTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGtggttggt                                                                                                      >  1:716026/1‑150 (MQ=255)
                                              gTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCATCACATACCGGACAGTCGTGCGGGtggttggt                                                                                                      >  1:1225524/1‑150 (MQ=255)
                                               tGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtc                                                                                                     <  1:404901/150‑1 (MQ=255)
                                               tGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtc                                                                                                     >  2:1014285/1‑150 (MQ=255)
                                                 agagaTGAATGGCCCCAAATCCTGCTTACGGTGGGTACGTTTGGTGAACCGGTAGCGACGGAAGCTGTGGCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtcat                                                                                                   <  2:255079/150‑1 (MQ=255)
                                                  gagaTGAATTGCCCCAAATCCTGATTAAGGTGGGTACGTATGGTGAAACTGTAGCGACGGAAGCTGTGTCAGGTCATCACAGTCATATACTGAAGATGGCAGTTACCGCCCTATTCACATACCGGACAGTCGTGCGGGTTGGTGGtcatc                                                                                                  >  1:80122/1‑150 (MQ=255)
                                                  gagaTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtcatc                                                                                                  >  1:1523030/1‑150 (MQ=255)
                                                     aTGAATGGCCCCTAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCTACGGAAGCTGTTTCCGGTCATCACAGTCATTTCCTGAAGATGGCAGTTACAGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAc                                                                                               <  1:1144459/150‑1 (MQ=255)
                                                        aaTGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAcccc                                                                                            >  1:153239/1‑148 (MQ=255)
                                                         aTGGCCCCACATCCTGATTACGGCGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCAcc                                                                                           <  2:1111914/150‑2 (MQ=255)
                                                          tGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTc                                                                                          <  2:1004638/150‑1 (MQ=255)
                                                           ggCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCACCCg                                                                                         <  1:706306/150‑1 (MQ=255)
                                                            gCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGa                                                                                        >  1:1473554/1‑150 (MQ=255)
                                                            gCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGa                                                                                        >  1:455159/1‑150 (MQ=255)
                                                             ccccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGAc                                                                                       <  1:1270512/150‑1 (MQ=255)
                                                             ccccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGAc                                                                                       <  1:1684608/150‑1 (MQ=255)
                                                               ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGAGTGGTTGATGACCAACCACCCgcacg                                                                                     <  2:8386/150‑5 (MQ=255)
                                                                      cTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTc                                                                              >  1:1427963/1‑150 (MQ=255)
                                                                      cTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATACTGAAGATGGCAGTTACCGCCCTCTGCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCACCCCCTCGCCCACGCTTTc                                                                              >  1:876759/1‑150 (MQ=255)
                                                                       tGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCa                                                                             >  1:689569/1‑150 (MQ=255)
                                                                        gATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCAc                                                                            >  2:941092/1‑150 (MQ=255)
                                                                           tACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGa                                                                         <  1:968272/150‑1 (MQ=255)
                                                                            aCGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGaa                                                                        <  2:1689472/150‑1 (MQ=255)
                                                                             cGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAAc                                                                       <  1:1824518/150‑1 (MQ=255)
                                                                                   gTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTc                                                                 <  1:485051/150‑1 (MQ=255)
                                                                                   gTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTc                                                                 <  2:1455889/150‑1 (MQ=255)
                                                                                     aCGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGc                                                               <  2:737489/150‑1 (MQ=255)
                                                                                           ggTGAAACGGTAGAGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                         >  2:1332544/1‑150 (MQ=255)
                                                                                            gTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcg                                                        >  2:1258732/1‑150 (MQ=255)
                                                                                               aaaCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcgtcg                                                     <  1:1703060/150‑1 (MQ=255)
                                                                                                      aGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATgg                                              <  2:660076/150‑1 (MQ=255)
                                                                                                        cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATTGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                            <  2:684821/150‑1 (MQ=255)
                                                                                                        cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                            >  2:1166084/1‑150 (MQ=255)
                                                                                                        cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                            >  1:1630674/1‑150 (MQ=255)
                                                                                                        cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                            <  1:550716/150‑1 (MQ=255)
                                                                                                         gACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaaa                                           <  1:1764784/150‑1 (MQ=255)
                                                                                                         gACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACCAATTAACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaaa                                           >  1:1626874/1‑150 (MQ=255)
                                                                                                          aCGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaaaa                                          >  1:1096874/1‑149 (MQ=255)
                                                                                                          aCGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAAt                                          <  2:1473554/150‑1 (MQ=255)
                                                                                                                gCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGt                                    >  1:36748/1‑150 (MQ=255)
                                                                                                                gCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGt                                    <  2:1595911/150‑1 (MQ=255)
                                                                                                                gCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGt                                    >  1:835107/1‑150 (MQ=255)
                                                                                                                  tgtgTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGAGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGc                                  >  1:964927/1‑150 (MQ=255)
                                                                                                                    tgtCCGGTCATCACTGTCATATCATGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCa                                <  2:270324/150‑1 (MQ=255)
                                                                                                                     gtCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGccct                               >  2:801903/1‑148 (MQ=255)
                                                                                                                       ccGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                             >  1:1232753/1‑150 (MQ=255)
                                                                                                                       caggTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATAACGTACAGTCGTGCGGTTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCTCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                             <  2:1273906/148‑1 (MQ=255)
                                                                                                                        cGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCgg                            <  1:1609402/150‑1 (MQ=255)
                                                                                                                         ggTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATCCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGa                           <  1:1351410/150‑1 (MQ=255)
                                                                                                                         ggTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGa                           <  2:1035580/150‑1 (MQ=255)
                                                                                                                          gTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGaa                          <  2:1763382/150‑1 (MQ=255)
                                                                                                                               cacaGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCgg                     >  2:1554218/1‑150 (MQ=255)
                                                                                                                               cacaGGCATTTCCTGAAGAGGGCAGTTACCGCCGTCTTCACATACCGGACAGTCGGGCGTGTGGTTTGGCATCAACCACTCGACCACGCTTTCTCGGAACTGTGTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCgg                     <  1:269127/150‑1 (MQ=255)
                                                                                                                                acaGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgt                    >  2:837497/1‑150 (MQ=255)
                                                                                                                                 caGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                   <  2:1626566/150‑1 (MQ=255)
                                                                                                                                     cATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAt               >  1:117691/1‑150 (MQ=255)
                                                                                                                                     aatatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTAGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTCTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAt               <  1:1526756/149‑1 (MQ=255)
                                                                                                                                         tCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAg           <  1:525993/150‑1 (MQ=255)
                                                                                                                                         atgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAg           >  2:765951/4‑150 (MQ=255)
                                                                                                                                         atgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCGTCGTCGTCAATGGAAATAATGGTGCCAGCGGAAGCCGGTGTCATACAg           >  1:239318/4‑150 (MQ=255)
                                                                                                                                          tgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg          >  1:260163/3‑150 (MQ=255)
                                                                                                                                          ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg          >  2:903014/1‑150 (MQ=255)
                                                                                                                                           cTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGa         >  2:1518096/1‑150 (MQ=255)
                                                                                                                                             gAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGGCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa       >  1:632354/1‑150 (MQ=255)
                                                                                                                                             gAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa       >  2:832914/1‑150 (MQ=255)
                                                                                                                                              aaGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACAt      <  2:1720633/150‑1 (MQ=255)
                                                                                                                                               aGATGGCATTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATc     <  2:1579479/150‑1 (MQ=255)
                                                                                                                                                gATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCa    >  1:494277/1‑150 (MQ=255)
                                                                                                                                                  tGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAcc  <  2:1156368/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACC  >  NC_000913/2399682‑2399977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑