Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,399,878 C→G 5.0% M96I (ATG→ATC nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,399,8780CG 5.0% 318.3 / 6.6 100M96I (ATG→ATCnuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base C (49/46);  new base G (2/3);  total (51/49)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 108 total aligned reads displayed.

TGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTG  >  NC_000913/2399729‑2400027
                                                                                                                                                     |                                                                                                                                                     
tGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtc                                                                                                                                                       <  1:404901/150‑1 (MQ=255)
tGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtc                                                                                                                                                       >  2:1049424/1‑150 (MQ=255)
tGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtc                                                                                                                                                       >  2:1014285/1‑150 (MQ=255)
  agagaTGAATGGCCCCAAATCCTGCTTACGGTGGGTACGTTTGGTGAACCGGTAGCGACGGAAGCTGTGGCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtcat                                                                                                                                                     <  2:255079/150‑1 (MQ=255)
   gagaTGAATTGCCCCAAATCCTGATTAAGGTGGGTACGTATGGTGAAACTGTAGCGACGGAAGCTGTGTCAGGTCATCACAGTCATATACTGAAGATGGCAGTTACCGCCCTATTCACATACCGGACAGTCGTGCGGGTTGGTGGtcatc                                                                                                                                                    >  1:80122/1‑150 (MQ=255)
   gagaTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtcatc                                                                                                                                                    >  1:1523030/1‑150 (MQ=255)
      aTGAATGGCCCCTAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCTACGGAAGCTGTTTCCGGTCATCACAGTCATTTCCTGAAGATGGCAGTTACAGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAc                                                                                                                                                 <  1:1144459/150‑1 (MQ=255)
         aaTGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAcccc                                                                                                                                              >  1:153239/1‑148 (MQ=255)
          aTGGCCCCACATCCTGATTACGGCGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCAcc                                                                                                                                             <  2:1111914/150‑2 (MQ=255)
           tGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTc                                                                                                                                            <  2:1004638/150‑1 (MQ=255)
            ggCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCACCCg                                                                                                                                           <  1:706306/150‑1 (MQ=255)
             gCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGa                                                                                                                                          >  1:455159/1‑150 (MQ=255)
             gCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGa                                                                                                                                          >  1:1473554/1‑150 (MQ=255)
              ccccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGAc                                                                                                                                         <  1:1684608/150‑1 (MQ=255)
              ccccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGAc                                                                                                                                         <  1:1270512/150‑1 (MQ=255)
                ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGAGTGGTTGATGACCAACCACCCgcacg                                                                                                                                       <  2:8386/150‑5 (MQ=255)
                       cTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATACTGAAGATGGCAGTTACCGCCCTCTGCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCACCCCCTCGCCCACGCTTTc                                                                                                                                >  1:876759/1‑150 (MQ=255)
                        tGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCa                                                                                                                               >  1:689569/1‑150 (MQ=255)
                            tACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGa                                                                                                                           <  1:968272/150‑1 (MQ=255)
                             aCGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGaa                                                                                                                          <  2:1689472/150‑1 (MQ=255)
                              cGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAAc                                                                                                                         <  1:1824518/150‑1 (MQ=255)
                                    gTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTc                                                                                                                   <  2:1455889/150‑1 (MQ=255)
                                    gTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTc                                                                                                                   <  1:485051/150‑1 (MQ=255)
                                      aCGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGc                                                                                                                 <  2:737489/150‑1 (MQ=255)
                                            ggTGAAACGGTAGAGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                                                           >  2:1332544/1‑150 (MQ=255)
                                             gTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcg                                                                                                          >  2:1258732/1‑150 (MQ=255)
                                                aaaCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcgtcg                                                                                                       <  1:1703060/150‑1 (MQ=255)
                                                       aGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATgg                                                                                                <  2:660076/150‑1 (MQ=255)
                                                         cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATTGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                                                                              <  2:684821/150‑1 (MQ=255)
                                                         cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                                                                              >  2:1166084/1‑150 (MQ=255)
                                                         cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                                                                              <  1:550716/150‑1 (MQ=255)
                                                         cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                                                                              >  1:1630674/1‑150 (MQ=255)
                                                          gACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACCAATTAACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaaa                                                                                             >  1:1626874/1‑150 (MQ=255)
                                                           aCGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaaaa                                                                                            >  1:1096874/1‑149 (MQ=255)
                                                           aCGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAAt                                                                                            <  2:1473554/150‑1 (MQ=255)
                                                                 gCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGt                                                                                      >  1:36748/1‑150 (MQ=255)
                                                                 gCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGt                                                                                      >  1:835107/1‑150 (MQ=255)
                                                                 gCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGt                                                                                      <  2:1595911/150‑1 (MQ=255)
                                                                     tgtCCGGTCATCACTGTCATATCATGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCa                                                                                  <  2:270324/150‑1 (MQ=255)
                                                                      gtCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGccct                                                                                 >  2:801903/1‑148 (MQ=255)
                                                                        ccGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                                                                               >  1:1232753/1‑150 (MQ=255)
                                                                        caggTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATAACGTACAGTCGTGCGGTTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCTCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                                                                               <  2:1273906/148‑1 (MQ=255)
                                                                         cGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCgg                                                                              <  1:1609402/150‑1 (MQ=255)
                                                                          ggTCATCACAGTCATATCCTGCAGATGGCAGTTACCGCCCACTTCACATACCGGACAGTCGTGCGGGTGGTTGGGCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGa                                                                             <  2:188869/150‑1 (MQ=255)
                                                                          ggTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATCCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGa                                                                             <  1:1351410/150‑1 (MQ=255)
                                                                          ggTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGa                                                                             <  2:1035580/150‑1 (MQ=255)
                                                                           gTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGaa                                                                            <  2:1763382/150‑1 (MQ=255)
                                                                                cacaGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCgg                                                                       >  2:1554218/1‑150 (MQ=255)
                                                                                cacaGGCATTTCCTGAAGAGGGCAGTTACCGCCGTCTTCACATACCGGACAGTCGGGCGTGTGGTTTGGCATCAACCACTCGACCACGCTTTCTCGGAACTGTGTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCgg                                                                       <  1:269127/150‑1 (MQ=255)
                                                                                 acaGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgt                                                                      >  2:837497/1‑150 (MQ=255)
                                                                                  caGTCATCTCCTGAAGAAGTCAGTGACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGGCATCAACCACTCGACCACGCTTTCACGGAACTGTCTCGCTACTTCGTCGTCAATGGAAAAAAAGGTGCCATCGGAAGCCGgtg                                                                     <  2:729214/150‑1 (MQ=255)
                                                                                      cATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAt                                                                 >  1:117691/1‑150 (MQ=255)
                                                                                      aatatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTAGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTCTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAt                                                                 <  1:1526756/149‑1 (MQ=255)
                                                                                         atCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACa                                                              <  1:1677140/150‑1 (MQ=255)
                                                                                         atCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACa                                                              >  1:444667/1‑150 (MQ=255)
                                                                                          atgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAg                                                             >  2:765951/4‑150 (MQ=255)
                                                                                          atgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCGTCGTCGTCAATGGAAATAATGGTGCCAGCGGAAGCCGGTGTCATACAg                                                             >  1:239318/4‑150 (MQ=255)
                                                                                           tgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                            >  1:260163/3‑150 (MQ=255)
                                                                                           ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                                            >  2:903014/1‑150 (MQ=255)
                                                                                            cTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGa                                                           >  2:1518096/1‑150 (MQ=255)
                                                                                              gAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGGCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa                                                         >  1:632354/1‑150 (MQ=255)
                                                                                              gAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa                                                         >  2:832914/1‑150 (MQ=255)
                                                                                               aaGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACAt                                                        <  2:1720633/150‑1 (MQ=255)
                                                                                                aGATGGCATTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATc                                                       <  2:1579479/150‑1 (MQ=255)
                                                                                                 gATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCa                                                      >  1:494277/1‑150 (MQ=255)
                                                                                                   tGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAcc                                                    <  2:1156368/150‑1 (MQ=255)
                                                                                                   tGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAcc                                                    >  1:763126/1‑150 (MQ=255)
                                                                                                    cgCAGTTACCGCCCTCTTCACATACCGTACAGTCGTGCGGGTGGTTGGTCATCAACCCCTCTACAACGCGTTCACGGAAATGTTTCGCTTCTTCGTCGTCAAAGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAAACAGGACATCAccc                                                   >  2:121241/2‑149 (MQ=255)
                                                                                                     gCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAg                                                  <  2:117691/150‑1 (MQ=255)
                                                                                                      cAGGTACCGCCCACTTCACCTACCCGCCAGGCGGGCGGGTGGTTGGGCATCAACCACGCGACCACGCTTTCCCCGAACTGTTTCGCTTCCTCGTCCTCAATGGAAATAAAGGTGCCATCGGAAGCCGGGGTCATACAGGACATCACCAgg                                                 <  1:1085535/150‑1 (MQ=255)
                                                                                                        gTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCg                                               >  1:1557929/1‑150 (MQ=255)
                                                                                                          tACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCATCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAc                                             <  1:943745/150‑1 (MQ=255)
                                                                                                            ccGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAccc                                           >  2:830470/1‑149 (MQ=255)
                                                                                                              gCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATGAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTTCCATCGGAAGCCGGTGTCATACAGGACAACACCAGGCGACCAcg                                         >  1:1144292/1‑150 (MQ=255)
                                                                                                               cccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCCCTCGCCCCCGCTTTCACGGAACTGTTTGGCGTGTTGGTCGTGAAAGGAAATAAAGGTGCCATCGGAAGCCGGGGGCCTAACGGTCATCACCACGCGCCCCcgc                                        >  2:173452/1‑150 (MQ=255)
                                                                                                                ccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGTAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAACGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgct                                       <  1:960645/150‑2 (MQ=255)
                                                                                                                ccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgcg                                       <  1:1085317/150‑1 (MQ=255)
                                                                                                                ccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTGTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAATGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgcg                                       >  1:103230/1‑150 (MQ=255)
                                                                                                                 ctctTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACCTCACCAGGCGACCAcgcgg                                      >  2:1506289/1‑149 (MQ=255)
                                                                                                                      cacaTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCtt                                 >  1:20310/1‑150 (MQ=255)
                                                                                                                      cacaTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCACTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCtt                                 >  2:825810/1‑150 (MQ=255)
                                                                                                                       acaTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTc                                <  2:1225524/150‑1 (MQ=255)
                                                                                                                          tACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGGGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCcgc                             <  2:1523030/150‑1 (MQ=255)
                                                                                                                           aCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGCCCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCcgcg                            >  1:419010/1‑150 (MQ=255)
                                                                                                                           aCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCcgcg                            >  1:1434315/1‑150 (MQ=255)
                                                                                                                            ccGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGt                           >  1:1202435/1‑150 (MQ=255)
                                                                                                                                     cGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt                  <  2:1427963/150‑1 (MQ=255)
                                                                                                                                     cGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt                  >  2:820254/1‑150 (MQ=255)
                                                                                                                                     cGTGCGGGGGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCGTCTTCGTCTTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACTGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt                  <  2:153239/150‑1 (MQ=255)
                                                                                                                                      gTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTAGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACAACGCGTGTCTTCCGCGTTTTGGTATTg                 >  2:769484/1‑150 (MQ=255)
                                                                                                                                       tGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGc                <  1:1258732/150‑1 (MQ=255)
                                                                                                                                        tcgagtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCt               >  2:1043470/5‑150 (MQ=255)
                                                                                                                                         cgagtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCtt              >  2:549071/4‑150 (MQ=255)
                                                                                                                                             tggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCAcc          >  1:791605/1‑150 (MQ=255)
                                                                                                                                               gttggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGc        <  1:1786764/150‑1 (MQ=255)
                                                                                                                                                  ggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTATTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTGCCGCGTTTTGGTATTGCTTCACCGcaca     >  2:360701/1‑150 (MQ=255)
                                                                                                                                                   gtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCacac    >  1:619363/1‑150 (MQ=255)
                                                                                                                                                   gtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGGGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCacac    <  1:1014285/150‑1 (MQ=255)
                                                                                                                                                     catcaACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCCCGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTg  >  2:180540/1‑150 (MQ=255)
                                                                                                                                                     catcaACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTg  >  2:21599/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCACACTG  >  NC_000913/2399729‑2400027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: