Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,407,898 A→C 7.1% Q113P (CAG→CCG) ‡ alaA → glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,407,8980AC 7.1% 364.0 / 15.7 127Q113P (CAG→CCG) ‡alaAglutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base A (56/62);  new base C (5/4);  total (61/66)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 129 total aligned reads displayed.

ACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCG  >  NC_000913/2407750‑2408047
                                                                                                                                                    |                                                                                                                                                      
 ccGCTCAAGGGTATTGCGATTCCATAGGGCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAgg                                                                                                                                                      <  1:311728/150‑1 (MQ=255)
 ccGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAgg                                                                                                                                                      >  2:1502831/1‑150 (MQ=255)
  cGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGc                                                                                                                                                     <  2:1651908/150‑1 (MQ=255)
    cTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCAt                                                                                                                                                   >  2:542458/1‑150 (MQ=255)
      cAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTg                                                                                                                                                 >  1:584747/1‑150 (MQ=255)
       aaGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGc                                                                                                                                               <  2:1364462/150‑1 (MQ=255)
       aaGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGc                                                                                                                                               <  2:732028/150‑1 (MQ=255)
        aGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCt                                                                                                                                              >  2:1273426/1‑150 (MQ=255)
         gggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCATGCTCGTGGCATGCGTGATGTTACCGTGGAATATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTg                                                                                                                                             <  2:40564/150‑1 (MQ=255)
         gggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTg                                                                                                                                             <  1:1020945/150‑1 (MQ=255)
         gggTAGTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCGCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCCTGCATTGcct                                                                                                                                             <  2:1026798/150‑3 (MQ=255)
          ggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCTGCAATGGTGTATCGGAGCTTATCGTTCAGTCAATGCAGGCATTGCTGa                                                                                                                                            >  1:883759/1‑150 (MQ=255)
           gTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAAAGCCTGCATTGcctga                                                                                                                                           <  1:1266329/150‑5 (MQ=255)
            tATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAAc                                                                                                                                          <  2:859102/150‑1 (MQ=255)
              ttGCGATTCCAAAGGTCTTTACTCCGCGCGT‑‑AGCCATCATGCAGCACTACCAGGCTCGTGGCCTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACTGCg                                                                                                                                      >  2:285122/1‑150 (MQ=255)
               tGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGCAATGCCTGCATTGCCTGAAcgat                                                                                                                                       <  2:698306/150‑4 (MQ=255)
                gCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCg                                                                                                                                      >  1:600562/1‑150 (MQ=255)
                 cGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCgg                                                                                                                                     >  1:1341220/1‑150 (MQ=255)
                      ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGCACAGCggggccc                                                                                                                                >  2:600859/1‑147 (MQ=255)
                      ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACg                                                                                                                                >  1:428729/1‑150 (MQ=255)
                      ccAAAGGTCTTTACTCAGCGAGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACg                                                                                                                                <  2:801278/150‑1 (MQ=255)
                             tCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGtt                                                                                                                         <  2:1435534/150‑1 (MQ=255)
                              cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                                        <  1:1374877/150‑1 (MQ=255)
                              cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                                        <  1:1502831/150‑1 (MQ=255)
                              cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGGGGCATGCGTGATGTTACCGTGGAATATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCATGCAATGCAGGCATTGCTGAACTGCGTGGACGAAATGTTg                                                                                                                        <  1:1464762/150‑1 (MQ=255)
                               tttACTGAGCTCGTAAAGCCTTCATGCTGGTCTTACAGGCTTGTGTCATTCGTGTTGTTTCTGTGGAAGATATTTAGATCGGCTTTGGTTTATCGGAGCTTATCGTTCAGGCTATGCAGGCATTTCGGATCAGATGGGACGAAATGTTgg                                                                                                                       <  2:328740/150‑1 (MQ=255)
                               tttACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATTCAGGCATTGCTGAACAGCGGGGACGAAATGTTgg                                                                                                                       <  1:1623122/150‑1 (MQ=255)
                               tttACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTgg                                                                                                                       <  2:328738/150‑1 (MQ=255)
                                 tactacGCGCGTATAGTCATAATGCAGCACTACCAGGGTCGTGGCATGCGTGATGTTACCGTGGAAGATATATACATCGGCAATGGGGTATCGGAGATTATCGTTCAGGAAATGGAGGCATTGCTGAACAGCGGGGACGTAATGTTGGtt                                                                                                                     <  2:1114990/150‑1 (MQ=255)
                                 tACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGTCATTGATGAACAGAGGGGACGAAAtgtttgtt                                                                                                                     >  2:1190269/1‑150 (MQ=255)
                                 tACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGtt                                                                                                                     <  1:1509620/150‑1 (MQ=255)
                                   cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                   >  1:12342/1‑150 (MQ=255)
                                   cTCCGCGCGGAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGGTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                   <  1:1315808/150‑1 (MQ=255)
                                      cgcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGTTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGc                                                                                                                <  1:1306994/150‑1 (MQ=255)
                                        cgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTAATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCAc                                                                                                              >  2:900651/1‑150 (MQ=255)
                                                gCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTAAGGCAATGCAGGCATTGCTGAACAGCGGGGAGGAAATGTTGGTTCCTGCAGCAGAttgc                                                                                                      >  2:1309340/1‑148 (MQ=255)
                                                 ccATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAcc                                                                                                     <  1:1095654/150‑1 (MQ=255)
                                                  catcatGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAccc                                                                                                    <  1:1369184/150‑1 (MQ=255)
                                                      atGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActc                                                                                                >  1:793762/1‑150 (MQ=255)
                                                      atGCAGCACTACCAGGCTCGTGGCATGCGTGAGGTAACCGTGGAAGATATTTACATCTGAATTGGTGTATCGGAGCGTATCGTTCAGGCAATGCAGGCATTGCTGTACAGCGGTGACGATATGTTGGTTCCTGCACCAGATTACCCActc                                                                                                <  1:1145951/150‑1 (MQ=255)
                                                       tGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActct                                                                                               >  2:854436/1‑150 (MQ=255)
                                                       tGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActct                                                                                               >  1:569631/1‑150 (MQ=255)
                                                           gcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGAc                                                                                           >  1:949310/1‑150 (MQ=255)
                                                            caCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGAcc                                                                                          >  2:1487955/1‑150 (MQ=255)
                                                               tACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACcgcg                                                                                       <  2:1048450/150‑1 (MQ=255)
                                                               tACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACcgcg                                                                                       <  2:1041516/150‑1 (MQ=255)
                                                                aCCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCACATTACCCACTCTGGACCgcgg                                                                                      >  2:1200225/1‑150 (MQ=255)
                                                                 ccAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCgcggc                                                                                     <  1:1136227/150‑1 (MQ=255)
                                                                 ccAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCgcggc                                                                                     <  1:1368143/150‑1 (MQ=255)
                                                                 ccAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCgcggc                                                                                     <  1:1145508/150‑1 (MQ=255)
                                                                 ccAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATAGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTTGTTGCTGCACCCGATAACCCACTCTGGACCgcggc                                                                                     >  2:893601/1‑150 (MQ=255)
                                                                       tCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCTTTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTc                                                                               <  1:473324/150‑1 (MQ=255)
                                                                       tCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTc                                                                               >  1:641641/1‑150 (MQ=255)
                                                                         gTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCATATTACCCACTCTGGACCGCGGCGGTTTCGc                                                                             <  1:1005432/150‑1 (MQ=255)
                                                                         gTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTAACGTTCAGCAAATCCAGGCATTGATGAACACCGGGCACGAATTTTTGTTACATGCCCCAGATACCCCAAGCTGGCCCGCGGCGGTTTCga                                                                             >  2:649263/1‑149 (MQ=255)
                                                                           ggCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCtt                                                                           <  1:1782394/150‑1 (MQ=255)
                                                                             cATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTc                                                                         <  1:834623/150‑1 (MQ=255)
                                                                                gCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAg                                                                      >  1:206923/1‑150 (MQ=255)
                                                                                 ggTGATGTTACCGTGGAAGATATTTAAATCGTCAATGGTTTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGc                                                                     <  1:634242/149‑1 (MQ=255)
                                                                                    gATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGt                                                                  >  2:708664/1‑150 (MQ=255)
                                                                                    gATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGt                                                                  >  2:532788/1‑150 (MQ=255)
                                                                                    gATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACACACTCTGGACCGCGGCGTTTTCGCTTTCCAGCGGt                                                                  >  2:566004/1‑150 (MQ=255)
                                                                                    gATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTCTCGTGCAGGCACTGCAGGCCTTGCGGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGt                                                                  <  1:1554040/150‑1 (MQ=255)
                                                                                     aTGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTa                                                                 >  1:597323/1‑150 (MQ=255)
                                                                                     aTGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTa                                                                 <  2:346240/150‑1 (MQ=255)
                                                                                      tGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTaa                                                                >  1:1760246/1‑150 (MQ=255)
                                                                                       gTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTaaa                                                               <  2:576864/150‑1 (MQ=255)
                                                                                          aCCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCg                                                            >  2:900976/1‑150 (MQ=255)
                                                                                           ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGTAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                           <  1:631638/150‑1 (MQ=255)
                                                                                           ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                           <  1:1681570/150‑1 (MQ=255)
                                                                                           ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCGGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                           >  2:1098758/1‑150 (MQ=255)
                                                                                              tGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGc                                                        <  2:859768/150‑1 (MQ=255)
                                                                                               ggAAGATATTTTCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCa                                                       <  1:965891/150‑1 (MQ=255)
                                                                                                 aaGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCATGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCAtt                                                     >  2:1149373/1‑150 (MQ=255)
                                                                                                 aaGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCAtt                                                     >  2:1720278/1‑150 (MQ=255)
                                                                                                  agagATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCATGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTTGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAATCGGTGCATTa                                                    >  1:397268/1‑150 (MQ=255)
                                                                                                  aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                                    >  1:1719502/1‑150 (MQ=255)
                                                                                                         ttgctCGGGAATGGTGTATGGGAGGTTATCGTTCTGGCAATGCAGGCATTGCTGAACAGGGGGGATGAAATGTTGGGTCCTGGACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                             <  1:1553296/146‑1 (MQ=255)
                                                                                                         aaCATCGGCAATGGTGTATCGGAGCTTATCGTTCACGCAATTCAGGCATTGCTGAACAGCGGTGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGCTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                             <  1:268698/149‑1 (MQ=255)
                                                                                                          aCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCg                                            <  1:449148/150‑1 (MQ=255)
                                                                                                             tCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGGTCCTGCACCAGATTACCCACTCTGGACCACGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATg                                         <  1:1273426/150‑1 (MQ=255)
                                                                                                                 cAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATc                                     <  2:1746061/150‑1 (MQ=255)
                                                                                                                   aTGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCct                                   <  2:239648/150‑1 (MQ=255)
                                                                                                                     ggTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGATTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCctct                                 >  1:1458921/1‑150 (MQ=255)
                                                                                                                         tATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGTCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACt                             <  1:566004/150‑1 (MQ=255)
                                                                                                                           tgggtgCTTATCGTTCAGGCAATGCAGGGATTGCTGAACAGAGGGGACGAAATGTTGGTTCCTGGACGAGATTACCCTCTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTgg                           <  1:1404537/145‑1 (MQ=255)
                                                                                                                           tCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGATTCCTCTGACTgg                           >  1:1674138/1‑150 (MQ=255)
                                                                                                                                 cccgctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCg                     >  1:1539990/7‑150 (MQ=255)
                                                                                                                                 cccgctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACACACACTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCg                     >  1:639438/7‑150 (MQ=255)
                                                                                                                                   cgctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGa                   >  1:1579416/5‑150 (MQ=255)
                                                                                                                                    gctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc                  >  1:1725057/4‑150 (MQ=255)
                                                                                                                                    aTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc                  >  2:1738254/1‑150 (MQ=255)
                                                                                                                                       gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc               >  1:1643632/1‑150 (MQ=255)
                                                                                                                                       gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc               <  1:1331222/150‑1 (MQ=255)
                                                                                                                                        ttCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCATCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTACCCGGCCCTCg              >  1:365080/1‑150 (MQ=255)
                                                                                                                                         tCAGGCAATACAGGCATTGCTGACCAGCGGGGACGAAATGTTGGTTCATGCACCAGATTACCCACTATTGCCCGCTGCGGTTTCGCTTTCCATCGGTAAAGCGGTGCATTATCTTTGCGTCGAATCCTCTGACTGGTTGCCGGACCTAga             >  2:225001/1‑150 (MQ=255)
                                                                                                                                          cAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGATCCTGCACCAGATTACCCACTCTGGACAGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTTCATTATCTTTGCGATGATTCCTCTGACTGGTTCCCGGCCCTCgat            >  1:1127402/1‑150 (MQ=255)
                                                                                                                                            ggCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgatga          <  1:900651/150‑1 (MQ=255)
                                                                                                                                                aTGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGCATCCGATGACTGGTTCCCGGACCTCGATGCTAtt      >  2:266885/1‑150 (MQ=255)
                                                                                                                                                   cAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTTGACCGCGGCGGTTTCGCTTTCCAGAGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCAGCCTGGTTCACGGACCTCGATGATATTcg  >  1:245894/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCG  >  NC_000913/2407750‑2408047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: