Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,413,009 T→A 5.5% K42* (AAG→TAG)  yfbV ← UPF0208 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,413,0090TA 5.5% 329.8 / 5.0 114K42* (AAG→TAG) yfbVUPF0208 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (56/50);  new base A (3/4);  total (60/54)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAG  >  NC_000913/2412863‑2413158
                                                                                                                                                  |                                                                                                                                                     
cAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGTCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGAt                                                                                                                                                    <  2:1765025/150‑1 (MQ=255)
cAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAAAACGGCGGACGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGAt                                                                                                                                                    >  1:1110841/1‑150 (MQ=255)
  gCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTGGAttc                                                                                                                                                  >  2:1681502/1‑148 (MQ=255)
  gCCACCACAATCCCTGCATGGGTAAACATAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATaa                                                                                                                                                  >  1:12855/1‑150 (MQ=255)
    caccacAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAAcg                                                                                                                                                >  1:1372160/1‑150 (MQ=255)
     accacAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAAcgc                                                                                                                                               >  1:1410604/1‑150 (MQ=255)
       cacaATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGa                                                                                                                                             >  2:30267/1‑150 (MQ=255)
        acaATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGAt                                                                                                                                            >  1:1213451/1‑150 (MQ=255)
        acaATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGAt                                                                                                                                            <  1:1467409/150‑1 (MQ=255)
            tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgttatgc                                                                                                                                        <  1:118160/150‑7 (MQ=255)
            tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTTATCAAGATGAcgcgttatgc                                                                                                                                        <  1:1484206/150‑7 (MQ=255)
            tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTc                                                                                                                                        <  2:1601130/150‑1 (MQ=255)
            tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTc                                                                                                                                        <  1:634818/150‑1 (MQ=255)
             cccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGTGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCg                                                                                                                                       <  2:1327705/150‑1 (MQ=255)
             cccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCg                                                                                                                                       <  2:1549405/150‑1 (MQ=255)
             cccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCg                                                                                                                                       <  2:144184/150‑1 (MQ=255)
               cTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGAc                                                                                                                                     <  1:1672523/150‑1 (MQ=255)
               cTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGAc                                                                                                                                     <  1:740169/150‑1 (MQ=255)
               cTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGAc                                                                                                                                     <  2:661479/150‑1 (MQ=255)
               cTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGAGCGCATAACGCGTCATCTTGATAACGCGATTTTCGAc                                                                                                                                     <  1:1198612/150‑1 (MQ=255)
                   aTGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAg                                                                                                                                 <  1:1240721/150‑1 (MQ=255)
                   aTGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAg                                                                                                                                 <  2:1744739/150‑1 (MQ=255)
                         aaaCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCggg                                                                                                                           <  1:868889/150‑1 (MQ=255)
                          aaCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCAAAACGCGTCATCTAGATAACGCGATTTTCGACAAAGACCgggg                                                                                                                          <  2:1090632/150‑1 (MQ=255)
                            cTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGCCAAAGACCGGGGCa                                                                                                                        >  1:934881/1‑150 (MQ=255)
                              tAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAg                                                                                                                      <  1:1034050/150‑1 (MQ=255)
                              tAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAg                                                                                                                      >  1:153846/1‑150 (MQ=255)
                              gaaGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCCGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGAATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCCACAAAGACCGGGGCAAg                                                                                                                      >  2:1171787/2‑150 (MQ=255)
                               aaGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAgg                                                                                                                     <  2:473942/150‑1 (MQ=255)
                                 ggCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCg                                                                                                                   >  2:1785815/1‑150 (MQ=255)
                                 ggCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCg                                                                                                                   >  1:1210370/1‑150 (MQ=255)
                                 ggCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCg                                                                                                                   >  2:1464216/1‑150 (MQ=255)
                                 ggCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCg                                                                                                                   <  1:1138053/150‑1 (MQ=255)
                                      ggccggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCGTCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                              >  1:1745835/5‑150 (MQ=255)
                                      aCAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                              <  1:860650/150‑1 (MQ=255)
                                      aCAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt                                                                                                              <  1:942126/150‑1 (MQ=255)
                                       cAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCTAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTc                                                                                                             <  1:576425/150‑1 (MQ=255)
                                       cAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTc                                                                                                             <  1:1298572/150‑1 (MQ=255)
                                        ccggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTcc                                                                                                            >  2:1266479/3‑150 (MQ=255)
                                          ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg                                                                                                          >  2:135105/1‑150 (MQ=255)
                                            cAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCg                                                                                                        <  1:424004/150‑1 (MQ=255)
                                             aGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCgg                                                                                                       <  2:1244949/150‑1 (MQ=255)
                                                ggCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGccc                                                                                                    >  2:169727/1‑149 (MQ=255)
                                                ggCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCa                                                                                                    >  1:1268619/1‑150 (MQ=255)
                                                  cAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACg                                                                                                  <  2:198681/150‑1 (MQ=255)
                                                   aaCTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGt                                                                                                 >  2:1005223/1‑150 (MQ=255)
                                                    aCTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTc                                                                                                >  1:1628780/1‑150 (MQ=255)
                                                      tGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCtt                                                                                              >  2:364140/1‑150 (MQ=255)
                                                        ccGGCCCTAGCTGAGCGCCCATGGCAATCTGCCATCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACATAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTg                                                                                            <  1:149524/150‑1 (MQ=255)
                                                        ccGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTg                                                                                            <  1:684869/150‑1 (MQ=255)
                                                        ccGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTg                                                                                            >  2:261765/1‑150 (MQ=255)
                                                        ccGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTg                                                                                            <  2:520010/150‑1 (MQ=255)
                                                        ccGGCCCAAGCTGACCGCCCAGGGCAACCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTg                                                                                            <  1:1374285/150‑1 (MQ=255)
                                                          ggCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAg                                                                                          <  1:1400149/150‑1 (MQ=255)
                                                            cccAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTATTCCAGCGGCCACGTCTTTGAGTa                                                                                        >  2:870943/1‑150 (MQ=255)
                                                                aGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGc                                                                                    <  2:1606322/150‑1 (MQ=255)
                                                                  cTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTg                                                                                  >  1:1187835/1‑150 (MQ=255)
                                                                  cTGACCGCCCAGGGCAATCTGAAAGCAGAGAGTATATACGGCGATCGGCGTCATAACACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCAAGTCTTTGAGTAATGCTg                                                                                  <  2:1094704/150‑1 (MQ=255)
                                                                   tGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGAGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGCCAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGt                                                                                 >  2:965619/1‑150 (MQ=255)
                                                                    gACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGCTTTTCGACACAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTc                                                                                >  2:340222/1‑150 (MQ=255)
                                                                     aCCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTcc                                                                               >  2:558733/1‑150 (MQ=255)
                                                                     aCCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTcc                                                                               >  2:512985/1‑150 (MQ=255)
                                                                      ccGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCAATTTTCGACAAAGCCCGGGGCAAGGCGTTATTCCCGCGGCCACGTCTTTGAGTAATGCTGTccc                                                                              >  1:1323382/1‑150 (MQ=255)
                                                                          ccAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCAGATAACGGCTCGCATAACGCGTCATCTAGATAACGCGAGTTTCGACAAACACCCGGGCAAGGCGTATTTCCAGCGGCCACGTCTGTGAGTAATGCTGTCCCCGGc                                                                          >  1:303020/1‑150 (MQ=255)
                                                                             gggCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGaa                                                                       <  2:181420/150‑1 (MQ=255)
                                                                               ccAATCTGCCAGCAGACAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGAACGGGGCAAGGCGTTTTACCAGCGGCCACGTCTTTGAGTAATGCTGTCACCGGCGAAAc                                                                     >  1:1221856/2‑150 (MQ=255)
                                                                                  aTCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATATAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAg                                                                  <  2:1534201/150‑1 (MQ=255)
                                                                                      gCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAAAGGAGCGCATACCGCGTCATCTTGTTAACGCGATTTTCGAAAAAGACCTGGGCAAGGCGGTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaa                                                              <  2:311690/150‑1 (MQ=255)
                                                                                       ccAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaa                                                             >  2:1158681/1‑150 (MQ=255)
                                                                                           cagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaaa                                                         >  2:1157406/1‑150 (MQ=255)
                                                                                           cagAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGATTATCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTaaaaaaa                                                         >  1:398926/1‑150 (MQ=255)
                                                                                              agagTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTa                                                      >  2:415966/1‑150 (MQ=255)
                                                                                              agagTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTa                                                      <  1:1005223/150‑1 (MQ=255)
                                                                                                agTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAAc                                                    <  1:46977/150‑1 (MQ=255)
                                                                                                agTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAAc                                                    >  1:1574552/1‑150 (MQ=255)
                                                                                                 gTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACa                                                   <  1:519823/150‑1 (MQ=255)
                                                                                                   aaaTACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGa                                                 >  2:666914/1‑150 (MQ=255)
                                                                                                      tACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACg                                              >  2:1260262/1‑150 (MQ=255)
                                                                                                        cGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGAt                                            >  2:718715/1‑150 (MQ=255)
                                                                                                               cggcggCATAAAACGGCTCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCgg                                     <  1:1166349/150‑1 (MQ=255)
                                                                                                               cggcggCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCgg                                     >  2:1197215/1‑150 (MQ=255)
                                                                                                               cggcggCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCgg                                     >  1:958857/1‑150 (MQ=255)
                                                                                                               cggcggCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCgg                                     >  1:1182839/1‑150 (MQ=255)
                                                                                                                      aTAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGAc                              >  2:1513459/1‑150 (MQ=255)
                                                                                                                      aTAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGAc                              >  2:216460/1‑150 (MQ=255)
                                                                                                                         aaaCGGATCGCATAACGCGTCTTCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATg                           <  2:1745835/150‑1 (MQ=255)
                                                                                                                         aaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATg                           >  2:219124/1‑150 (MQ=255)
                                                                                                                         aaaCGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGCGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTTACATg                           >  2:193764/1‑150 (MQ=255)
                                                                                                                           aCGGCTCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCt                         <  2:559179/150‑1 (MQ=255)
                                                                                                                            cGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCtt                        >  1:978686/1‑150 (MQ=255)
                                                                                                                              gATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCa                      <  2:847832/150‑1 (MQ=255)
                                                                                                                                  gaaaatcgcgTTATCAAGATGACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                  >  1:485849/7‑150 (MQ=255)
                                                                                                                                  gaaaatcgcgTTATCAAGATGACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                  >  1:697190/7‑150 (MQ=255)
                                                                                                                                  gCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                  >  1:299432/1‑150 (MQ=255)
                                                                                                                                  gCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTc                  >  2:1558787/1‑150 (MQ=255)
                                                                                                                                   cATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCa                 >  1:806385/1‑150 (MQ=255)
                                                                                                                                     tAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAAc               <  2:1176546/150‑1 (MQ=255)
                                                                                                                                     tAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAAc               >  2:1276218/1‑150 (MQ=255)
                                                                                                                                                  tGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAg  >  2:546903/1‑150 (MQ=255)
                                                                                                                                                  tGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGAGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAg  <  1:30267/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGTCTTTGAGTAATGCTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAG  >  NC_000913/2412863‑2413158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑