Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,615 C→G 5.2% G145G (GGG→GGC) ‡ yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6150CG 5.2% 494.6 / 14.5 155G145G (GGG→GGC) ‡yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (61/86);  new base G (3/5);  total (64/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

Only 100 of 157 total aligned reads displayed.

ATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACT  >  NC_000913/2449469‑2449764
                                                                                                                                                   |                                                                                                                                                     
 tcaAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCt                                                                                                                                                   <  2:214304/150‑1 (MQ=255)
 tcaAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCt                                                                                                                                                   <  2:17447/150‑1 (MQ=255)
  caAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCACCTg                                                                                                                                                  >  2:1424758/1‑150 (MQ=255)
   aaaTACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGa                                                                                                                                                 >  1:896198/1‑150 (MQ=255)
    aaTACGTTGAAAGCGCTTTCGCACCCCCACCGCCCCGCGACTCCAGGGTGAGCCGGTTTGACTTACTGACATAATTCTCCTTCCATGACACGTCATAAGGTTTAACCGAAACGGATGGACAAAGCGTGTCGTGTCTATCAGGCCCCTGAt                                                                                                                                                <  2:167492/150‑1 (MQ=255)
      tACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATAc                                                                                                                                              <  1:542529/150‑1 (MQ=255)
          ttGAAATCGATTTCTCAACAACACCGCCCGTCGACTGCATGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATagtca                                                                                                                                           <  2:1589540/150‑5 (MQ=255)
          ttGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGGGCCTGATAGTCAc                                                                                                                                         <  1:1344044/150‑1 (MQ=255)
          ttGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCa                                                                                                                                          >  1:822438/1‑150 (MQ=255)
            gAAAGCTTTTTCTAAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg                                                                                                                                        <  1:1621447/150‑1 (MQ=255)
            gAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg                                                                                                                                        >  2:1187635/1‑150 (MQ=255)
             aaaGCGTTTTCTCACCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCAATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTCACAGGCCCCTGATACGTCACGc                                                                                                                                       >  2:120234/1‑150 (MQ=255)
             aaaGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACga                                                                                                                                       <  1:61781/150‑2 (MQ=255)
             aaaGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGc                                                                                                                                       >  1:791657/1‑150 (MQ=255)
                     ttCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGga                                                                                                                               <  1:939078/150‑1 (MQ=255)
                      tctcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgag                                                                                                                              <  1:1785627/150‑1 (MQ=255)
                      tctcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCAGATACGTCACGCCCCCTGgag                                                                                                                              >  2:1625555/1‑150 (MQ=255)
                       ctcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                                                                             >  2:1104289/1‑150 (MQ=255)
                       ctcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                                                                             >  2:1303069/1‑150 (MQ=255)
                       ctcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                                                                             >  1:1195954/1‑150 (MQ=255)
                       ctcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCAAAAGGTTGAACAGAAACGGATAGACAAATCGTGTCGTGCCTATCAGCCCCCTGAAACGTCACGCCCCCTGgagc                                                                                                                             >  2:1127622/1‑149 (MQ=255)
                        tcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                                                                            >  2:215451/1‑150 (MQ=255)
                           aCCACACCGCCCGTCGAGTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCt                                                                                                                         >  1:1720917/1‑150 (MQ=255)
                           aCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCt                                                                                                                         <  1:380841/150‑1 (MQ=255)
                             cacaCCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTc                                                                                                                       <  1:1018062/150‑1 (MQ=255)
                             cacaCCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACAAAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTc                                                                                                                       <  2:1704063/150‑1 (MQ=255)
                              acacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGACCCCTGATACGTCACGCCCCCTGGAGAGGCTTCa                                                                                                                      >  2:1206982/1‑150 (MQ=255)
                               cacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                                                                                     >  1:873969/1‑150 (MQ=255)
                                 ccGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCTCCTGGAGAGGCTTCagag                                                                                                                   <  2:958653/150‑1 (MQ=255)
                                 ccGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                                                                   >  1:298515/1‑150 (MQ=255)
                                 ccGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                                                                   >  2:180425/1‑150 (MQ=255)
                                   gCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAAGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtc                                                                                                                 <  2:792549/150‑1 (MQ=255)
                                    cccGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                                                                                >  1:1216432/1‑150 (MQ=255)
                                         cGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                                                           >  2:1287290/1‑150 (MQ=255)
                                         cGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                                                           >  2:544948/1‑150 (MQ=255)
                                            cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataat                                                                                                        >  2:1365944/1‑150 (MQ=255)
                                                   gTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaac                                                                                                 <  2:140300/150‑1 (MQ=255)
                                                     gtgCAGTGTTGACTTACTGAAATAATTCTCATTCCAGGAAAGGTCTTAAGCTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCGCTGGAGAGGCTTCAGAGTCATTCATAATAaccaacca                                                                                               <  2:345747/148‑1 (MQ=255)
                                                      aGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAt                                                                                              >  1:309118/1‑150 (MQ=255)
                                                       gCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATg                                                                                             <  2:1484211/150‑1 (MQ=255)
                                                       gCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATg                                                                                             >  1:365442/1‑150 (MQ=255)
                                                        cagggttGACTTACTGACATAATTCGCATTCCATGAAACGTCATAAGGTCGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATgg                                                                                            <  2:1113974/145‑1 (MQ=255)
                                                          gTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                                                                          >  2:639981/1‑150 (MQ=255)
                                                            tttGACTTACTGACATAATTCTCATTCCATGAAACTTCATAAGGTTTAACAGAAACTGATGGACAAATCGTGTCTTGACTATCAGGCCCCTGATACGTCACGCCCCATTGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACaa                                                                                        <  1:313496/150‑1 (MQ=255)
                                                            tttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACaa                                                                                        <  1:354353/150‑1 (MQ=255)
                                                              tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGa                                                                                      <  1:1606073/150‑1 (MQ=255)
                                                                 cTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGaaaa                                                                                   <  1:730631/150‑1 (MQ=255)
                                                                  ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                                                                                  <  2:1551815/150‑1 (MQ=255)
                                                                   tACTGACATAATTCTCATTCCATGAAAAGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGTCTTCAGAGTCATTCATAATAACCAACCATGGACCAGAAAAGt                                                                                 <  2:65423/150‑1 (MQ=255)
                                                                    aCTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                                                                                <  2:1011895/150‑1 (MQ=255)
                                                                      tGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAg                                                                              >  1:620175/1‑150 (MQ=255)
                                                                      tGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAg                                                                              <  1:654450/150‑1 (MQ=255)
                                                                      tGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGAGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAg                                                                              <  1:462468/150‑1 (MQ=255)
                                                                        aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                                                                                       <  2:419017/123‑1 (MQ=255)
                                                                        aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                                                                                       >  1:419017/1‑123 (MQ=255)
                                                                         cATAATTCTCATTCCATGAAACGTAATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAtt                                                                           >  2:1658365/1‑150 (MQ=255)
                                                                          aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                          <  1:1292767/150‑1 (MQ=255)
                                                                          aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTAATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                          >  1:1076189/1‑150 (MQ=255)
                                                                            aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTCTCGTGACTATCAGGCCCCTGATACGTCACGCCCCATGGATAGGCTTCAGACTGATTCATAATAACCACCCCTGGAGAAGAAAAGTAAGCTTCaa                                                                        >  2:91629/1‑150 (MQ=255)
                                                                             tttCTCATTCCATGAAACGTCATATGGTTGATCAGAATCGGTTGGACTAATCGTGTCGTGACTTTCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGGTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAt                                                                       <  2:503632/149‑1 (MQ=255)
                                                                                 tcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCATGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct                                                                   <  2:381760/150‑1 (MQ=255)
                                                                                  cATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg                                                                  <  2:896198/150‑1 (MQ=255)
                                                                                  cATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg                                                                  <  1:124308/150‑1 (MQ=255)
                                                                                         tGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc                                                           <  1:699483/150‑1 (MQ=255)
                                                                                         tGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc                                                           <  2:927788/150‑1 (MQ=255)
                                                                                         tGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc                                                           <  2:733038/150‑1 (MQ=255)
                                                                                           aaaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACaa                                                         <  2:681073/150‑1 (MQ=255)
                                                                                            aaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAAt                                                        <  1:1017647/150‑1 (MQ=255)
                                                                                                tCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc                                                    <  2:822438/150‑1 (MQ=255)
                                                                                                 cATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCt                                                   >  2:1281813/1‑150 (MQ=255)
                                                                                                 cATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCt                                                   >  2:236939/1‑150 (MQ=255)
                                                                                                  aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTc                                                  >  2:506887/1‑150 (MQ=255)
                                                                                                   tAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCa                                                 >  2:163900/1‑150 (MQ=255)
                                                                                                   tAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCACAGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTAAGCAAAGACAATAACCTCa                                                 >  1:1370983/1‑150 (MQ=255)
                                                                                                      ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAAAAACCAACCATGGACAAGAAAAGTAAGATTCAATATCTGAAGAGACAATAACCTCAAAt                                              >  2:776067/1‑150 (MQ=255)
                                                                                                          gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtat                                          <  1:1494832/150‑1 (MQ=255)
                                                                                                          gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtat                                          <  1:1648459/150‑1 (MQ=255)
                                                                                                          gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTCGAGAGGCTTCAGAGTCATTCATAATAACCACCCATGGACAAGAAAAGTAAGATTAAATCTCTGCACAGCAAATAACCTCAAATGtat                                          >  1:994092/1‑150 (MQ=255)
                                                                                                           aaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtata                                         <  1:180425/150‑1 (MQ=255)
                                                                                                           aaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtata                                         <  2:309118/150‑1 (MQ=255)
                                                                                                           aaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtata                                         <  1:1187635/150‑1 (MQ=255)
                                                                                                           aaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAACGtata                                         <  1:1424758/150‑1 (MQ=255)
                                                                                                               gAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCGTCAGAGTCATTCATAATAACCCACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATAc                                     >  1:1112528/1‑150 (MQ=255)
                                                                                                                 aaCGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCAGTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATAccc                                   >  1:1680728/1‑150 (MQ=255)
                                                                                                                        ggACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTc                            <  2:657991/150‑1 (MQ=255)
                                                                                                                         gACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATTTATAATACCAGCAGttac                           >  2:1100624/1‑148 (MQ=255)
                                                                                                                          aCAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCa                          <  1:1622217/150‑1 (MQ=255)
                                                                                                                           cAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCAc                         >  2:648491/1‑150 (MQ=255)
                                                                                                                            aaaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGCCAATAACCTCAAATGTATAATACCCGCAGTTCCAcc                        >  2:1218647/1‑150 (MQ=255)
                                                                                                                             aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCAccc                       <  1:1303069/150‑1 (MQ=255)
                                                                                                                                   gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACgg                 <  1:1365944/150‑1 (MQ=255)
                                                                                                                                     cgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGATACAATAACCTCAAATCTATAATACCCTCAGTTACACCCAGACGGTa               >  2:1218687/1‑150 (MQ=255)
                                                                                                                                       tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACATCAAATGTATAAAAACCTCAGTTCCACCCAGACGGTAAt             >  2:79004/1‑150 (MQ=255)
                                                                                                                                          cTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCa          >  2:1435774/1‑150 (MQ=255)
                                                                                                                                          cTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATAACCGCAGTTCCACCCAGACGGTAATCCa          >  1:353133/1‑150 (MQ=255)
                                                                                                                                             aTCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAgg        <  2:304052/150‑1 (MQ=255)
                                                                                                                                              tCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGa       >  2:590096/1‑150 (MQ=255)
                                                                                                                                                 ggCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACaa    <  2:1463813/150‑1 (MQ=255)
                                                                                                                                                  gCCCCTGTTACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAAc   <  2:878637/150‑1 (MQ=255)
                                                                                                                                                   ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACt  <  1:236939/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACT  >  NC_000913/2449469‑2449764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: