Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,464,228 C→A 7.7% intergenic (+158/+24) yfdF → / ← mlaA uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,464,2280CA 7.7% 311.5 / 11.4 115intergenic (+158/+24)yfdF/mlaAuncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
Reads supporting (aligned to +/- strand):  ref base C (55/50);  new base A (4/6);  total (59/56)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 115 total aligned reads displayed.

TCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCA  >  NC_000913/2464080‑2464373
                                                                                                                                                     |                                                                                                                                                 
tCCAGTTTTTTGATTTTTCAACATCTGAATATCGGAAATAGTCAGTGACGAACATCAGTTTTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGGTTTGCg                                                                                                                                                  <  1:960218/150‑1 (MQ=255)
tCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTAATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCg                                                                                                                                                  <  2:1642772/150‑1 (MQ=255)
 ccAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGa                                                                                                                                                 >  1:1634233/1‑150 (MQ=255)
  cAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGAc                                                                                                                                                <  1:557753/150‑1 (MQ=255)
  cAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGAc                                                                                                                                                >  1:848716/1‑150 (MQ=255)
    gTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTc                                                                                                                                              >  1:674581/1‑150 (MQ=255)
       ttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCAcc                                                                                                                                           >  1:1715261/1‑150 (MQ=255)
        tttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCt                                                                                                                                          <  1:858854/150‑1 (MQ=255)
         ttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtt                                                                                                                                         <  1:192098/150‑1 (MQ=255)
          tGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCttt                                                                                                                                        >  1:805908/1‑150 (MQ=255)
           gATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                       >  1:155312/1‑150 (MQ=255)
            aTTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCttttt                                                                                                                                      <  2:845717/150‑1 (MQ=255)
            aTTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttt                                                                                                                                      <  2:246526/150‑1 (MQ=255)
              ttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTa                                                                                                                                    >  1:725196/1‑150 (MQ=255)
               tttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttttgt                                                                                                                                   <  1:1089150/150‑3 (MQ=255)
                ttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAtt                                                                                                                                  <  1:1148871/150‑1 (MQ=255)
                 tCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAttt                                                                                                                                 <  1:1277083/150‑1 (MQ=255)
                 tCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCTTTTTTGttg                                                                                                                                 <  1:177632/150‑2 (MQ=39)
                  cAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTg                                                                                                                                >  1:1422821/1‑150 (MQ=255)
                  cAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCTTTTTTGttgt                                                                                                                                <  1:406545/150‑3 (MQ=38)
                    aCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGtt                                                                                                                              >  2:687999/1‑150 (MQ=255)
                    aCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGtt                                                                                                                              >  2:1459438/1‑150 (MQ=255)
                     cAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                             >  1:340161/1‑150 (MQ=255)
                      aGCTGAATATCTTAAAAAGTCAGTGATGAATATCAGTATTTTTACATAAATACGTCTAGGTCTTTGTCGGGTTATCTCATTTTACTTGTGGTAATTGAGTGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTc                                                                                                                            <  2:306552/150‑1 (MQ=255)
                       gCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCt                                                                                                                           >  2:248899/1‑150 (MQ=255)
                          gAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTAt                                                                                                                        >  1:755227/1‑150 (MQ=255)
                               tCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGa                                                                                                                   <  1:930038/150‑1 (MQ=255)
                                 ggAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATAGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAAt                                                                                                                 >  2:1408327/1‑150 (MQ=255)
                                  gAATTAGTCAGTGACGAACATCATTATTTATACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGTTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATc                                                                                                                <  2:651420/150‑1 (MQ=255)
                                    aaTAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCaa                                                                                                              <  2:812272/150‑1 (MQ=255)
                                    aaTAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCaa                                                                                                              <  1:262555/150‑1 (MQ=255)
                                      tAGTCAGTGACGAACATCAGTATTTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTAATTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAat                                                                                                             >  2:1462318/1‑150 (MQ=255)
                                          cAGTGAAGAACATCAGTATTTTTACATTATTACGTCTAGGTCTTTGTCGGTTTATCTCCTTTTAATTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtt                                                                                                        <  2:1435455/150‑1 (MQ=255)
                                           aGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCttt                                                                                                       >  2:539286/1‑150 (MQ=255)
                                            gTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtttt                                                                                                      <  2:1207320/150‑1 (MQ=255)
                                              gACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAATAAAGGTGAGTTTTGAGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaa                                                                                                    >  2:216450/1‑150 (MQ=255)
                                               aCGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaaa                                                                                                   >  2:1151857/1‑150 (MQ=255)
                                                cGAACATCAGTATTTTTACATAATTACGTCTATGTCCTTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAat                                                                                                  <  1:863316/150‑1 (MQ=255)
                                                cGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGGCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAat                                                                                                  <  1:423430/150‑1 (MQ=255)
                                                  aaCATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGAGACTCACCTATTTTATTTGTTTCGAATTCAGAATCAATATCTTTTAAatca                                                                                                >  1:1619934/1‑150 (MQ=255)
                                                    aaTCAGTATTTTTACATAATTACGTCTAGGTAATTGTCGGTTTATGTCCTTTTACTTGTGGTTATTGAGTGATAACAACAAAATAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGATTCAATATCTTTTAAatcatc                                                                                              <  1:918049/149‑1 (MQ=255)
                                                               tttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCt                                                                                    >  2:1049988/1‑150 (MQ=255)
                                                               tttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAAACGCt                                                                                    >  2:373145/1‑150 (MQ=255)
                                                               tttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTATTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTATGCGCATCACAATTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCt                                                                                    >  2:1670351/1‑150 (MQ=255)
                                                                ttACATAATTACGTCTAGGGCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCtt                                                                                   <  1:1367495/150‑1 (MQ=255)
                                                                     tAATTAAGTCTAGGTCATTGTATGTTTATCTCCTTTTACTTGTTGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCTGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCg                                                                              <  1:1343424/150‑1 (MQ=255)
                                                                       aTTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTTTGCGtt                                                                            >  2:1413740/1‑150 (MQ=255)
                                                                        ttACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTc                                                                           <  1:1338210/150‑1 (MQ=255)
                                                                         tACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCg                                                                          <  1:1794461/150‑1 (MQ=255)
                                                                             tCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGAtt                                                                      <  1:106347/150‑1 (MQ=255)
                                                                                     aTTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCg                                                              >  2:1435348/1‑150 (MQ=255)
                                                                                        gTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGtt                                                           >  1:391089/1‑150 (MQ=255)
                                                                                        gTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGtt                                                           >  1:397831/1‑150 (MQ=255)
                                                                                        gTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGtt                                                           >  2:845900/1‑150 (MQ=255)
                                                                                          ctgTTTATATCCTTTTACTTGTGGTAATTGAGTGATAAAAACAAAAAAGGTGAGTTTTGCGACTAACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTg                                                         <  2:358173/148‑1 (MQ=255)
                                                                                          cGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTg                                                         >  1:733230/1‑150 (MQ=255)
                                                                                          cGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                        <  2:1761302/150‑1 (MQ=255)
                                                                                           ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                        >  2:623677/1‑150 (MQ=255)
                                                                                           ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAg                                                       <  1:67007/150‑1 (MQ=255)
                                                                                            gTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAg                                                       <  2:340161/150‑1 (MQ=255)
                                                                                             tttATCTCCTTTTTCTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGt                                                      <  2:936256/150‑1 (MQ=255)
                                                                                                ttctcCTTTTTCTTGTGGTAATTGAGGGATAACAACAAAAAAGTTGAGTTTTGATACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAATTCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGGGGTTTGAGTTcg                                                   <  1:1704112/149‑1 (MQ=255)
                                                                                                aTCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGCCGGACCTTGTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCGTGTGCGTTCGGATTTTCCTGCGGTGTGAGTTcg                                                   >  2:329933/1‑150 (MQ=255)
                                                                                                 tctcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgc                                                  >  1:1557729/1‑150 (MQ=255)
                                                                                                 tctcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGAGTTCGGATTTTCCTGCGGTTTGAGTTcgc                                                  >  1:1301125/1‑150 (MQ=255)
                                                                                                  ctcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTTCGGTTTGAGTTcgcc                                                 >  2:1777131/1‑150 (MQ=255)
                                                                                                   tcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgccg                                                <  2:1685059/150‑1 (MQ=255)
                                                                                                   tcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgccg                                                <  1:248899/150‑1 (MQ=255)
                                                                                                      ttttACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCa                                             <  2:705448/150‑1 (MQ=255)
                                                                                                           cTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCTGTTTGAGTTCTCCGCAATTAGc                                        >  1:49670/1‑150 (MQ=255)
                                                                                                           cTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTAACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGc                                        <  2:1094465/150‑1 (MQ=255)
                                                                                                            ttGTGGTAATTGAGGGATAACAAAAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCAATAGCg                                       <  1:1099956/150‑1 (MQ=255)
                                                                                                               tgGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATg                                    <  2:674090/150‑1 (MQ=255)
                                                                                                               tgGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATg                                    >  1:1605508/1‑150 (MQ=255)
                                                                                                                 gTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGaa                                  >  1:337460/1‑150 (MQ=255)
                                                                                                                  tAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGaaa                                 >  1:984063/1‑150 (MQ=255)
                                                                                                                   acttGAGGGATAACAACAAACAAGCTGAGTATTCCGACACACCTTTTTTATTTGTATCTTATTCAGAATCAATATCATTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGTTTTGAGTTCGCCGCCATTAGCGATGAAAt                                <  1:286464/148‑1 (MQ=255)
                                                                                                                   aaTTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGaagt                                >  1:1459966/1‑148 (MQ=255)
                                                                                                                     ttGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTTGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCa                              <  2:755227/150‑1 (MQ=255)
                                                                                                                     ttGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCa                              <  1:1398707/150‑1 (MQ=255)
                                                                                                                        aGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGa                           <  1:858176/150‑1 (MQ=255)
                                                                                                                         gggATAACAACAAAAAAGGTGAGTTTTGCGACTCAGCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGAc                          >  1:1814936/1‑150 (MQ=255)
                                                                                                                         gggATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGCTTTGAGTTCGCCGCCATTAGCGATGAAATCATGAc                          >  2:1636400/1‑150 (MQ=255)
                                                                                                                          ggATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACg                         >  1:373182/1‑150 (MQ=255)
                                                                                                                            aTAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCt                       <  1:539286/150‑1 (MQ=255)
                                                                                                                              acaaataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTgg                     >  2:1761784/7‑150 (MQ=38)
                                                                                                                               acaacaAAATAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGa                    <  2:256060/150‑1 (MQ=255)
                                                                                                                                caacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGaa                   >  1:1443077/1‑150 (MQ=255)
                                                                                                                                caacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGaa                   >  1:1578795/1‑150 (MQ=255)
                                                                                                                                caacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGaa                   >  2:440201/1‑150 (MQ=255)
                                                                                                                                caacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCATGCGGTTTGAGTTAGCCGCCATTAGCGATGAAATCATGACGCTGGaa                   >  2:344347/1‑150 (MQ=255)
                                                                                                                                 aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                  >  2:1305424/1‑150 (MQ=255)
                                                                                                                                 aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCCTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                  >  2:40544/1‑150 (MQ=255)
                                                                                                                                  acaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGt                 <  2:848716/150‑1 (MQ=255)
                                                                                                                                   caaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGATTCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTa                >  1:378935/1‑150 (MQ=255)
                                                                                                                                       aaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCt            <  2:1109922/150‑1 (MQ=255)
                                                                                                                                          ggTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTcc         >  2:1820404/1‑149 (MQ=255)
                                                                                                                                            tGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATAACTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCAGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTcgcg       <  2:1634233/150‑1 (MQ=255)
                                                                                                                                              aGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCa     >  1:1231079/1‑150 (MQ=255)
                                                                                                                                                 tttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCa  >  2:1471618/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCA  >  NC_000913/2464080‑2464373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: