Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,477,014 C→A 8.6% A206S (GCA→TCA)  dsdC ← dsd operon activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,0140CA 8.6% 165.5 / 4.6 65A206S (GCA→TCA) dsdCdsd operon activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (11/46);  new base A (4/2);  total (16/49)
Fisher's exact test for biased strand distribution p-value = 2.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCA  >  NC_000913/2476865‑2477162
                                                                                                                                                     |                                                                                                                                                    
tcttcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGc                                                                                                                                                      <  1:1753900/150‑1 (MQ=255)
tcttcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGc                                                                                                                                                      <  1:1747005/150‑1 (MQ=255)
 cttcCCATCGCCACCCCAATATTATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGcc                                                                                                                                                     <  1:47453/150‑1 (MQ=255)
   tcCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctg                                                                                                                                                   <  2:887088/150‑1 (MQ=255)
             aCCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATgg                                                                                                                                         <  2:241630/150‑1 (MQ=255)
              ccccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGa                                                                                                                                        <  1:1234847/150‑1 (MQ=255)
               cccAATATGATTCATCGCGGCAATAACAGCTAAATCAGGACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAg                                                                                                                                       <  2:738542/150‑1 (MQ=255)
               cccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAg                                                                                                                                       <  2:885690/150‑1 (MQ=255)
                ccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGc                                                                                                                                      <  1:784281/150‑1 (MQ=255)
                   atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAAc                                                                                                                                   <  2:188909/150‑1 (MQ=255)
                     atGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGt                                                                                                                                 <  2:1481715/150‑1 (MQ=255)
                        aTTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCTGCAAGGCGGTGGGTCCTGGATCAAccgtcc                                                                                                                              >  2:323279/1‑145 (MQ=255)
                               gcgGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGacac                                                                                                                       <  2:1153943/150‑1 (MQ=255)
                                cgGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGAcaca                                                                                                                      <  1:783243/150‑1 (MQ=255)
                                    aaTAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTATTCGCATAATGTTGCGACCAACTATTCCATTCATCCGTACCGGAGTCGTTGCTCCATTCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGtt                                                                                                                  <  1:1117707/150‑1 (MQ=255)
                                        aCAGCTAAATCATAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAAtt                                                                                                              <  2:1099800/150‑1 (MQ=255)
                                        aCAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAAtt                                                                                                              >  2:1214581/1‑150 (MQ=255)
                                                aTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGtt                                                                                                      <  1:1062167/150‑1 (MQ=255)
                                                   aGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGt                                                                                                   <  1:1325467/150‑1 (MQ=255)
                                                    gAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGGCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGtt                                                                                                  <  1:1693757/150‑1 (MQ=255)
                                                      aCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTaa                                                                                                <  1:913541/150‑1 (MQ=255)
                                                       cGATCAAAGACAATTACAGAAGATGTCGGCAAATTCACCGCATAATGTTGCGACCACCTATGCCATTCATCCGTACCGGAGTCCTTTCAACAGGCCAGTGAGGCAAGGAGCAAACTAGAGTTACCAAAGGTAATTAACCTTGTGGTTaaa                                                                                               >  2:314787/1‑150 (MQ=255)
                                                        gATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAg                                                                                              <  2:570338/150‑1 (MQ=255)
                                                         ataaaaGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCCTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGc                                                                                             <  2:180862/147‑1 (MQ=255)
                                                         aTCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGc                                                                                             <  1:967433/150‑1 (MQ=255)
                                                         aTCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCGGTATGTAATTGGGCAACCGACAGTGACACAGGGTAATTTCCGGGGTGGTTTAAGc                                                                                             >  1:194921/1‑150 (MQ=255)
                                                                  aaTTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGAGAAATAACAGTAAGTCCTGGTGCAACGGACAGTGGCCCCCGTTAAGTACCCGGTAGGGTAAAGCAttgagttt                                                                                    >  2:275269/1‑143 (MQ=255)
                                                                    ttCCAGTAGATGTGGGCTTATTAACCGCTTAATTTTGCGCAGTACTATGCTATTCATCCTTACCGTAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                  <  1:279831/150‑1 (MQ=255)
                                                                    ttCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                  <  1:1450602/150‑1 (MQ=255)
                                                                      ccAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCg                                                                                <  1:1214581/150‑1 (MQ=255)
                                                                               gTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCTCCATTCCAGTATGGGAAGGGGCAAAGGACAGTGACACATGTTAATTACCCTGTTGGTTTAATCATGTCTTTGAGCGTAATGCggg                                                                       >  2:76460/1‑150 (MQ=255)
                                                                                tCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                      <  1:512489/150‑1 (MQ=255)
                                                                                tCGGCAAATTAACCGCATAATGTTGCGCCAAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATTGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                      <  1:757395/150‑1 (MQ=255)
                                                                                  ggCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTa                                                                    <  1:1444349/150‑1 (MQ=255)
                                                                                   gCAATTTATCCGCTTAATGTTGCGTCCAACTATGCCATTCATGCGTAGCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGGAACGTACAGTGACACAGGGTAATTACCGTGTTGGTTAAAGCATGGCTTTGAGCGTATTCCGGGCTAc                                                                   <  1:113215/150‑1 (MQ=255)
                                                                                    cAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGGCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACa                                                                  <  2:1613665/150‑1 (MQ=255)
                                                                                      aaTTAACCTCATAATCTTGAGCCCAACTATGCCATTCATCCTTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATa                                                                <  2:508801/150‑1 (MQ=255)
                                                                                      aaTTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATa                                                                <  2:1431979/150‑1 (MQ=255)
                                                                                                 tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATTCCTGTCGGTCATGGAGCACAGGACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCCTGTCAATGAGCGTATTCCGGGCGACATAATGGCAggtgt                                                     >  1:1286869/1‑147 (MQ=255)
                                                                                                 tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAtt                                                     <  2:907587/150‑1 (MQ=255)
                                                                                                    tGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttct                                                  <  2:825618/150‑1 (MQ=255)
                                                                                                    tGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCAGGGTGTGAAGGTGCAACGTACAGTGACACAGGTTAATTTCCGTGTTGGTTAAAGCCATTATTTGAGCGTTTTGCGGGCTACATACTGGCATGAtttat                                                  >  2:197582/1‑148 (MQ=255)
                                                                                                       tGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTca                                               <  2:516133/150‑1 (MQ=255)
                                                                                                         cgcCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatc                                             <  2:1320561/150‑1 (MQ=255)
                                                                                                         cgcCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatc                                             <  1:1556793/150‑1 (MQ=255)
                                                                                                         cgcCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCACCCTGCCTGTCTGGAATGGAGCAACGTACAGTGCCCCCGGGTAATTACCGTGTTGGTTAAAGCATGGAGTTGAGGGTAGTCCGGGCTACCAACGGGGATGATTTGTTcatc                                             >  2:288690/1‑150 (MQ=255)
                                                                                                         cgaccAAATATTCCATTCATCAGTACCTGAGTATTTTCTAAATGACTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatc                                             <  2:1313345/147‑1 (MQ=255)
                                                                                                           cccAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatcca                                           <  1:413367/150‑1 (MQ=255)
                                                                                                           cccAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGGCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatcca                                           <  2:610360/150‑1 (MQ=255)
                                                                                                              aaCTATGCCAATAATACGTACCGGAGGCGTTGCTCCATGCCTGTCTGTGATGGAGCAACGTACAGTGACACAGGGTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCa                                        <  1:323862/150‑1 (MQ=255)
                                                                                                               aCTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAg                                       <  1:1572725/150‑1 (MQ=255)
                                                                                                               aCTATGCCATTCATCCGTACCGGAGTCGTTGCTACAAGCCAGTATGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGGTGGGTAAAGCATGTGGTTGAGCGGTTTCCGGGCTACCTACTGGCAGGATTTCTTCATGCCTCAg                                       >  1:269389/1‑150 (MQ=255)
                                                                                                                 tATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACTGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCTTCCATCAGaa                                     <  2:1685355/150‑1 (MQ=255)
                                                                                                                    gCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGt                                  <  2:866593/150‑1 (MQ=255)
                                                                                                                        ttCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatg                              <  2:1714401/150‑1 (MQ=255)
                                                                                                                        ttCATCAGTACCGGAGTCGTTGCTCCATGGCTGTCTCTCATGGAGCAACGTACAGTGACACAGGTTAATTAGCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatg                              <  2:1145736/150‑1 (MQ=255)
                                                                                                                               gTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATgagtgagt                       <  2:1203156/150‑1 (MQ=255)
                                                                                                                                    ggAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgcgc                  <  2:875887/150‑1 (MQ=255)
                                                                                                                                         cGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg             <  1:1462720/150‑1 (MQ=255)
                                                                                                                                         cGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg             >  2:1651297/1‑150 (MQ=255)
                                                                                                                                             gCTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGGTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAGTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc         >  1:1248915/1‑150 (MQ=255)
                                                                                                                                             gCTCCATGACAGACAGGCATGGAGCAACGAACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTACGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc         >  1:541801/1‑150 (MQ=255)
                                                                                                                                              cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca        <  2:1448070/150‑1 (MQ=255)
                                                                                                                                              cTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTAAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca        >  1:513016/1‑150 (MQ=255)
                                                                                                                                               tCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcat       >  1:1666037/1‑150 (MQ=255)
                                                                                                                                               tCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcat       >  1:976253/1‑150 (MQ=255)
                                                                                                                                                   tGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCatcatc   <  2:1491668/150‑1 (MQ=255)
                                                                                                                                                    gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCCGGATTGCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactcac  <  1:1128944/150‑7 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCA  >  NC_000913/2476865‑2477162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: