Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,326 A→G 5.2% T24A (ACA→GCA)  yffL → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,3260AG 5.2% 342.2 / 5.1 113T24A (ACA→GCA) yffLCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (47/60);  new base G (4/2);  total (51/62)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGAC  >  NC_000913/2560177‑2560474
                                                                                                                                                     |                                                                                                                                                    
gTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCaa                                                                                                                                                      >  1:1761056/1‑150 (MQ=255)
gTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCaa                                                                                                                                                      <  1:135088/150‑1 (MQ=255)
     gCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGc                                                                                                                                                 >  1:1417439/1‑150 (MQ=255)
     gCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGc                                                                                                                                                 <  1:1708038/150‑1 (MQ=255)
           cttttTCGCTAATTATTCCCTTATTCCAATTAAGTGGAATAAATTATTCAAAATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggggg                                                                                                                                           <  2:1437740/150‑1 (MQ=255)
           cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggggg                                                                                                                                           >  2:730472/1‑150 (MQ=255)
           atttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggggg                                                                                                                                           <  1:1645766/149‑1 (MQ=255)
             ttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCg                                                                                                                                         <  1:679925/150‑1 (MQ=255)
              tttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                                                                        <  1:1556006/150‑1 (MQ=255)
               ttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGt                                                                                                                                       <  2:347948/150‑1 (MQ=255)
                 cGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTAt                                                                                                                                     >  2:1422910/1‑150 (MQ=255)
                  gCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                                                                                   <  1:1749283/150‑1 (MQ=255)
                   cTAATTATTCCATTATTCCAATTAAGTGTAATAAATTATTCAATATCAAGAACTTAACTCCATGTTTCCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                                                                                   <  1:254929/150‑1 (MQ=255)
                   cTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAATAACTTAAATACATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                                                                                   <  1:707100/150‑1 (MQ=255)
                      attattccattattccAATTAAGTTGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGaa                                                                                                                                <  2:1354463/150‑1 (MQ=255)
                        tattccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAAc                                                                                                                              <  1:1679650/150‑1 (MQ=255)
                           tccattattccaATTAAGTGTAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGTAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAg                                                                                                                           <  2:455780/150‑1 (MQ=255)
                             cattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGATTCCAACGAAAGATCAAGAGTTCGAAAATATGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGCGGCGGTATGAGAAACAAGCt                                                                                                                         >  1:1183894/1‑150 (MQ=255)
                              attattccaATTAAGTGTAATAAATTATTAAATATCAATAACTTACATCCATGTTTGCAAAGAAAGATCCAGAGTTCGAAAATAGTATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTa                                                                                                                        <  2:96705/150‑1 (MQ=255)
                                 attccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAg                                                                                                                     <  1:679691/150‑1 (MQ=255)
                                 attccaATTAAGTGGAATAAATTAATCAATATAAAGAACTTAAATCCATGATTGCAACGAAAGAACCAGAGTTCGAAAAAAGGATCAACACAAATAAAAGCCACAGGAATGCAGCAACATGCCGGGGGAGGTATGAGAAACAAGCTaacc                                                                                                                     >  1:2161/1‑148 (MQ=255)
                                  ttccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                    <  2:16918/150‑1 (MQ=255)
                                  ttccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                    <  2:1761056/150‑1 (MQ=255)
                                   tccaATTAAGTTGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGga                                                                                                                   <  2:1397093/150‑1 (MQ=255)
                                      aaTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                                                                                >  2:605311/1‑150 (MQ=255)
                                          aaGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAATTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtc                                                                                                            <  1:549724/150‑1 (MQ=255)
                                           aGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtct                                                                                                           >  2:1518958/1‑150 (MQ=255)
                                            gTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtctc                                                                                                          <  2:512684/150‑1 (MQ=255)
                                             tGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                                                                         >  2:1692658/1‑150 (MQ=255)
                                                   aaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTg                                                                                                   <  1:1001357/150‑1 (MQ=255)
                                                   aaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTg                                                                                                   <  2:18110/150‑1 (MQ=255)
                                                   aaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTg                                                                                                   <  2:632439/150‑1 (MQ=255)
                                                    aaTTATTCAATATCAAGAACTTAAATACATGTTTGAAACGAAATATCCATAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGa                                                                                                  <  1:808126/150‑1 (MQ=255)
                                                     attattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                                                                                 <  2:328560/150‑1 (MQ=255)
                                                                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAGAGGAGAATTTCTCATGTCTGATATGCTAGCAGt                                                                                      <  1:1818166/150‑1 (MQ=255)
                                                                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGt                                                                                      >  1:393365/1‑150 (MQ=255)
                                                                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGt                                                                                      >  1:1703052/1‑150 (MQ=255)
                                                                      aCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACAAGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACa                                                                                <  2:293638/150‑1 (MQ=255)
                                                                       cTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAACGCCCCCGGAATGCTGCAGCATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCAGGTCGGAGATGCTAGCAGTAGAACaa                                                                               >  2:260541/1‑150 (MQ=255)
                                                                       cTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACaa                                                                               <  1:692997/150‑1 (MQ=255)
                                                                       attAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACaa                                                                               <  1:1680695/149‑1 (MQ=255)
                                                                         tAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGa                                                                             <  1:1512303/150‑1 (MQ=255)
                                                                          aaaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                                                                            <  1:229843/150‑1 (MQ=255)
                                                                            aTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaac                                                                          >  1:439034/1‑150 (MQ=255)
                                                                             tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATAAACACAAATAAAAGCCACCGGAATGCTGCAACATGCCGGGGGCGGTATCAGAAACAAGCTAAAGGAGAATTTCTCATGTCTCATATGCTAGCAGTAGAACAAGaaaca                                                                         >  1:107484/1‑150 (MQ=255)
                                                                               cATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaaa                                                                       >  1:505600/1‑150 (MQ=255)
                                                                                 tGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAaacaaaca                                                                     >  1:764806/1‑150 (MQ=255)
                                                                                 tGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCACATGTCTGATATGCTAGCAGTAGAACAAGAaacaaaca                                                                     >  2:898902/1‑150 (MQ=255)
                                                                                   tttGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACAAAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAat                                                                   <  2:248345/150‑1 (MQ=255)
                                                                                       cAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACACGCTAAAGGAGCATTTCGCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgatg                                                               >  2:1123981/1‑150 (MQ=255)
                                                                                         aCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGtt                                                             >  2:28729/1‑150 (MQ=255)
                                                                                           gAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCg                                                           <  2:1579717/150‑1 (MQ=255)
                                                                                            aaaGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGt                                                          >  2:1473475/1‑150 (MQ=255)
                                                                                             aaGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGta                                                         >  2:485328/1‑149 (MQ=255)
                                                                                              aGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCa                                                        >  1:1656375/1‑150 (MQ=255)
                                                                                               gATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAATCAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCCTCaa                                                       >  2:204761/1‑150 (MQ=255)
                                                                                                aTCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAt                                                      >  1:1581166/1‑150 (MQ=255)
                                                                                                aTCCAGAGTTCGAAAATAGGATCAACACAAATAAAACCCACCGGAATGATGCAACATGCCGGGGGAGTTATGAGAAACAAGCAAAAGGAGAATAACTCATGTCTGAAATGCTAGCAGTAGAACAAGAAACAAACAATGAAGTTCTTCAAt                                                      >  2:416705/1‑150 (MQ=255)
                                                                                                 tCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAtt                                                     >  2:1399383/1‑150 (MQ=255)
                                                                                                      agTTCGTAAATAGGATCATCACAAATAAAAGCCCCCGGATTGCTGTAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaa                                                <  1:1444781/150‑1 (MQ=255)
                                                                                                      agTGCGAAAATAGGATCAACACAAATAAAAGCCCCAGGAATGCTGCTACATGCAGTGGGCGGTATGATAAACAAGCTAAAGGAGAATTTCTAATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaa                                                <  1:271686/150‑1 (MQ=255)
                                                                                                       tttCGAAAATAGGATCAACACAAATATAAGCGCCCGGAATGCTGCAACATGCCGGGGGCTGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                               <  2:1349574/149‑1 (MQ=255)
                                                                                                       gTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                               >  2:236676/1‑150 (MQ=255)
                                                                                                       gTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                               <  1:1418621/150‑1 (MQ=255)
                                                                                                        ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAAc                                              <  2:1259976/150‑1 (MQ=255)
                                                                                                        ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAAc                                              <  1:1579817/150‑1 (MQ=255)
                                                                                                         tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACa                                             <  2:464133/150‑1 (MQ=255)
                                                                                                          cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaa                                            >  2:557505/1‑150 (MQ=255)
                                                                                                          cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaa                                            <  2:1680286/150‑1 (MQ=255)
                                                                                                           gAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaaa                                           >  2:257117/1‑150 (MQ=255)
                                                                                                                  ggATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACg                                    >  1:812305/1‑150 (MQ=255)
                                                                                                                  ggATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACg                                    >  2:1167617/1‑150 (MQ=255)
                                                                                                                   gATCAACAAAAATAAAAGCCCACGGAATGCTGCAAAATGCCGGTTGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGa                                   <  1:61102/150‑1 (MQ=255)
                                                                                                                    aTCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAg                                  >  1:1150250/1‑150 (MQ=255)
                                                                                                                       aaCACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAACCAAAATTAACGAGTTa                               >  1:318373/1‑150 (MQ=255)
                                                                                                                       aaCACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTa                               >  2:1383782/1‑150 (MQ=255)
                                                                                                                       aaCACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAAGGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTa                               >  1:867980/1‑150 (MQ=255)
                                                                                                                         cacaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACg                             >  1:616335/1‑150 (MQ=255)
                                                                                                                          acaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACgg                            <  2:949593/150‑1 (MQ=255)
                                                                                                                          acaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACgg                            <  1:1216150/150‑1 (MQ=255)
                                                                                                                          acaAATAAAAGCCCCCGGAATGCTGAAACATTCAGGGGGAGGTATGAGAAACAATCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCTATTTTTAAACAAAATTAACGAGTTACgg                            <  1:56274/150‑1 (MQ=255)
                                                                                                                           caAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGa                           >  2:260138/1‑150 (MQ=255)
                                                                                                                               tAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATaa                       <  2:764806/150‑1 (MQ=255)
                                                                                                                               tAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATaa                       >  1:1318287/1‑150 (MQ=255)
                                                                                                                               tAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATaa                       <  1:1422027/150‑1 (MQ=255)
                                                                                                                               ataccgCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATaa                       >  1:174514/6‑150 (MQ=255)
                                                                                                                                aaaaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATaaa                      >  2:1562767/1‑150 (MQ=255)
                                                                                                                                  aaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGc                    <  2:1178335/150‑1 (MQ=255)
                                                                                                                                     cccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAcc                 >  1:274864/1‑150 (MQ=255)
                                                                                                                                     cccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAcc                 <  2:727637/150‑1 (MQ=255)
                                                                                                                                      ccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAccc                >  1:399328/1‑150 (MQ=255)
                                                                                                                                      ccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAccc                >  1:948169/1‑150 (MQ=255)
                                                                                                                                        ccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCaa              >  2:1416154/1‑150 (MQ=255)
                                                                                                                                             aTGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACg         <  2:466061/150‑1 (MQ=255)
                                                                                                                                              tgctgcAACTTGCCGGGGGAGGTATGTGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGAAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGa        <  2:1183894/150‑1 (MQ=255)
                                                                                                                                              tgctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGa        <  1:169716/150‑1 (MQ=255)
                                                                                                                                                ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAag      <  2:1150250/150‑1 (MQ=255)
                                                                                                                                                ctgcAACATGCCGGGGGAGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAag      <  2:544326/150‑1 (MQ=255)
                                                                                                                                                 tgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACTAGTTACGGAATAAAGCACCCAAAAACGAaga     >  2:1095599/1‑150 (MQ=255)
                                                                                                                                                    aaCATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAATTTAACGAGTTACGGAATAAAGCACCCAAAAACGAagagta  >  1:823532/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGAC  >  NC_000913/2560177‑2560474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: