Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,593,004 C→T 7.6% intergenic (+42/+68) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,593,0040CT 7.6% 319.9 / 10.3 105intergenic (+42/+68)ypfN/ypfHputative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base C (37/60);  new base T (6/2);  total (43/62)
Fisher's exact test for biased strand distribution p-value = 6.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAA  >  NC_000913/2592857‑2593153
                                                                                                                                                   |                                                                                                                                                     
tAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCAt                                                                                                                                                     >  1:1561728/1‑150 (MQ=255)
 aaGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc                                                                                                                                                    >  2:1771067/1‑150 (MQ=255)
  aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                                                                   <  1:1636298/150‑1 (MQ=255)
   gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCg                                                                                                                                                  <  2:829009/150‑1 (MQ=255)
    aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCgg                                                                                                                                                 <  1:1772731/150‑1 (MQ=255)
    aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCgg                                                                                                                                                 <  1:833390/150‑1 (MQ=255)
      aTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCa                                                                                                                                               <  2:407367/150‑1 (MQ=255)
        ttCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCACCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                                                                                                                             <  1:1151547/150‑1 (MQ=255)
            cGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTg                                                                                                                                         >  2:1059393/1‑150 (MQ=255)
            cGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTg                                                                                                                                         <  2:542620/150‑1 (MQ=255)
             gCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGa                                                                                                                                        <  2:1711361/150‑1 (MQ=255)
             gCCAACAAGCCGGAGCTTCCACCGCATCGTGATTTCAACGATAAGTGTGACGATGACGACGACTGGCCGAAAAATGATCAACCGAATAAGTAAGCTGAGTGCTCAACAGTAGTCCGGATAAGTCGTTAACGCATCATCAGGCAATGGTGa                                                                                                                                        >  2:355210/1‑150 (MQ=255)
              ccAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGaa                                                                                                                                       >  2:755746/1‑150 (MQ=255)
               caacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                                                                                                                      <  1:1376993/150‑1 (MQ=255)
                aacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACg                                                                                                                                     >  2:547982/1‑150 (MQ=255)
                   aaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATg                                                                                                                                  <  1:1397611/150‑1 (MQ=255)
                      ccGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATACGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCt                                                                                                                               >  1:1148528/1‑150 (MQ=255)
                       cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTg                                                                                                                              <  2:496244/150‑1 (MQ=255)
                        ggAGCTTCCCCCGAATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACAGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGa                                                                                                                             >  1:577743/1‑150 (MQ=255)
                          aGCTTCCCCCGCATGGTGATTTCCTCGATAAGGGGGACGATGACGACGACTGGCCGAAAATGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATg                                                                                                                           <  1:891673/150‑1 (MQ=38)
                             ttCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGa                                                                                                                        >  1:563642/1‑150 (MQ=255)
                                 cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCt                                                                                                                    >  1:564289/1‑150 (MQ=255)
                                  ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTc                                                                                                                   >  1:1382046/1‑150 (MQ=255)
                                   cGCCTCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCa                                                                                                                  <  1:317380/150‑1 (MQ=255)
                                   cGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGCCTGGCCGAAAAAGGATCAACCGAAGAAGAAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCa                                                                                                                  <  2:1209481/150‑1 (MQ=255)
                                      aTCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcgc                                                                                                               <  2:750121/150‑1 (MQ=255)
                                       tCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcgcg                                                                                                              <  1:1380329/150‑1 (MQ=255)
                                        cGTGATTTCAACGATAAGTGGGACGATGCAGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTACCGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGt                                                                                                             >  2:1372757/1‑150 (MQ=255)
                                         gaggttacAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGCTCAACAGAAGAAGTAAGCTGAGTGCTCATCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTc                                                                                                            <  1:339738/143‑1 (MQ=38)
                                         gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTc                                                                                                            <  1:1280589/150‑1 (MQ=255)
                                         gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATTGTGAACGATGCCTGATGCGACGCTCACGCGTc                                                                                                            <  2:21449/150‑1 (MQ=255)
                                         gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTc                                                                                                            >  1:93691/1‑150 (MQ=255)
                                         gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTc                                                                                                            <  1:1081498/150‑1 (MQ=255)
                                          tGATTTCAACGATAAGTGGGACGATGCCGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCt                                                                                                           >  1:977599/1‑150 (MQ=255)
                                              ttCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                       <  1:1552567/150‑1 (MQ=255)
                                                 aaCGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAgg                                                                                                    <  2:1391258/150‑1 (MQ=255)
                                                  aCGATAAGTGGGACGATGACGACGACTGGCCGAACAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGc                                                                                                   <  1:1446793/150‑1 (MQ=255)
                                                    gATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCa                                                                                                 <  1:1468959/150‑1 (MQ=255)
                                                    gATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCa                                                                                                 <  2:742932/150‑1 (MQ=255)
                                                     attaGTGGGACGTTGACTACGACGGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGATCACGCGTCTTATCAGGCCAg                                                                                                <  2:926725/147‑1 (MQ=37)
                                                      tAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAgg                                                                                               <  2:1697216/150‑1 (MQ=255)
                                                      aaaGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGACGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAgg                                                                                               <  1:259350/149‑1 (MQ=255)
                                                       aaGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGt                                                                                              <  1:1703475/150‑1 (MQ=255)
                                                         gTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTaa                                                                                            >  2:1510954/1‑150 (MQ=255)
                                                            ggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCt                                                                                         >  2:188615/1‑150 (MQ=255)
                                                               cGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTc                                                                                      >  2:1671729/1‑150 (MQ=255)
                                                                gATTACGACGACTGGCCGAAAAAGGATCAACCGAAGAACTAAGCTGAGTGCTCTACCGGAGGCCGGATACGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCt                                                                                     <  2:315729/150‑1 (MQ=38)
                                                                   tacgactacTGGCAGAAAACGTATCAACCGAAGAAGTATGCTGAGTGCTCAACAGTCGGCCGGATAAGGCGTGAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGAAGCTCTCGCGTCTTAGCAGGCCAGGTAATCTGTCtttt                                                                                  <  2:1093439/149‑1 (MQ=37)
                                                                   gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCtttt                                                                                  <  2:657246/150‑1 (MQ=255)
                                                                     cgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTTGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAc                                                                                <  1:1255134/150‑1 (MQ=255)
                                                                     cgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAc                                                                                <  1:1822010/150‑1 (MQ=255)
                                                                              gCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCa                                                                       <  1:1314000/150‑1 (MQ=255)
                                                                               ccGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCaa                                                                      >  2:799623/1‑150 (MQ=255)
                                                                                cGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAAt                                                                     <  2:156056/150‑1 (MQ=255)
                                                                                 gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATc                                                                    <  2:135101/150‑1 (MQ=255)
                                                                                  aaaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATca                                                                   <  1:1059393/150‑1 (MQ=255)
                                                                                   aaaaGGATCACCCGAAGAAGTCAGCTGAGTGCTCAACCGTCGGCCGGATACGTCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATcac                                                                  <  2:1221389/150‑1 (MQ=38)
                                                                                   aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATcac                                                                  >  2:708904/1‑150 (MQ=255)
                                                                                      aGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatc                                                               <  2:1486485/150‑1 (MQ=255)
                                                                                       ggATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAGTCAcatca                                                              >  1:1329392/1‑150 (MQ=255)
                                                                                       ggATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatca                                                              >  1:1617284/1‑150 (MQ=255)
                                                                                        gATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGGCTTTTACATCATTTCAATCAcatcat                                                             >  2:464107/1‑150 (MQ=255)
                                                                                         aTCCACCGAAGAAGTAAGCGGAGGGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatc                                                            <  2:143072/150‑1 (MQ=255)
                                                                                         aTCAACCGAAGAAGTAAGCTGAGTGCTCACCCGTCGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatc                                                            <  2:918933/150‑1 (MQ=255)
                                                                                         aTCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTCAACGATGCCTGATGCGACGCTCCCGCGTCTTAGCAGGCCAGTTAAGCTGTCTTTTACATCATTTCAAGCCcaacatc                                                            >  1:350685/1‑150 (MQ=25)
                                                                                         aTCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCCGCAATGTTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCATTTACATCATTTCAATCAcatcatc                                                            >  1:115794/1‑150 (MQ=255)
                                                                                            aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCCTCCGGCAATGGTGAACGATGCCTGATGCGACGCTCCCGCGTCTTATCAGGCCAGGTAATCTGTCTGTTACATCATTTCAATCACatcatcatc                                                         >  1:141760/1‑150 (MQ=255)
                                                                                             aCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCg                                                        <  2:563642/150‑1 (MQ=255)
                                                                                                gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCa                                                     >  2:413036/1‑150 (MQ=255)
                                                                                                gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCa                                                     <  1:1336522/150‑1 (MQ=255)
                                                                                                 aagaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTTAACGATGCCTGATGCGACGCTCACGCTTATTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCTCCAg                                                    <  1:1137142/150‑1 (MQ=255)
                                                                                                         aGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCg                                            <  1:1404289/150‑1 (MQ=255)
                                                                                                          gCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGAAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCCGGCCAGGTAATCTGTCGTTTACATCATTTCAATCCCATCATCATCGCCAGGCTTACCGc                                           >  2:84235/1‑150 (MQ=255)
                                                                                                              aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACt                                       <  1:956210/150‑1 (MQ=255)
                                                                                                                 gCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAg                                    >  1:1789382/1‑150 (MQ=255)
                                                                                                                  cTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                                   >  1:261292/1‑150 (MQ=255)
                                                                                                                    cAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcgc                                 >  2:30366/1‑150 (MQ=255)
                                                                                                                      aCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCtt                               <  1:1771067/150‑1 (MQ=255)
                                                                                                                         cattgCCGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAAGCCCATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgt                            >  1:296896/5‑150 (MQ=255)
                                                                                                                          tAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgtc                           <  2:92209/150‑1 (MQ=255)
                                                                                                                          attgCCGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATTCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgtc                           >  2:767653/4‑150 (MQ=255)
                                                                                                                          attgCCGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgtc                           >  1:1028380/4‑150 (MQ=255)
                                                                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCACTCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaa                        >  1:1195104/1‑150 (MQ=255)
                                                                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCCTCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaa                        >  1:1626131/1‑150 (MQ=255)
                                                                                                                             gCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACAACATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaa                        <  2:1382046/150‑1 (MQ=255)
                                                                                                                              ccGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaaa                       >  2:1506027/1‑150 (MQ=255)
                                                                                                                               cGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaat                      >  1:600814/1‑150 (MQ=255)
                                                                                                                                 gATAAGGCGTTAACGCCGCATCCGGCAATGGCGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaataa                    >  2:1800936/1‑150 (MQ=255)
                                                                                                                                        cGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCg             <  1:156612/150‑1 (MQ=255)
                                                                                                                                         gTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCgg            <  1:678781/150‑1 (MQ=255)
                                                                                                                                         gTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCgg            <  2:1148528/150‑1 (MQ=255)
                                                                                                                                            aaCGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAAt         >  2:485233/1‑150 (MQ=255)
                                                                                                                                            aaCGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAAt         <  1:1747156/150‑1 (MQ=255)
                                                                                                                                              cgccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCTCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATgg       >  2:1534000/1‑150 (MQ=255)
                                                                                                                                              cgccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATgg       >  1:1605695/1‑150 (MQ=255)
                                                                                                                                              cgccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATgg       <  1:812758/150‑1 (MQ=255)
                                                                                                                                              cgccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATgg       <  1:547982/150‑1 (MQ=255)
                                                                                                                                              cgccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATgg       <  1:39563/150‑1 (MQ=255)
                                                                                                                                                  gcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGtata   <  2:1014621/150‑1 (MQ=255)
                                                                                                                                                   cATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATaa  >  1:1473598/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAA  >  NC_000913/2592857‑2593153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: