Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,673,783 G→A 6.0% intergenic (+15/+33) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,7830GA 6.0% 372.7 / 8.2 117intergenic (+15/+33)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
Reads supporting (aligned to +/- strand):  ref base G (60/50);  new base A (5/2);  total (65/52)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

Only 100 of 119 total aligned reads displayed.

CCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGT  >  NC_000913/2673636‑2673930
                                                                                                                                                   |                                                                                                                                                   
ccATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcg                                                                                                                                                   >  2:1714530/1‑150 (MQ=255)
    tACTGGGATATGACCGGCGATGCGGTTGGGCCAATTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGt                                                                                                                                               <  1:1821302/150‑1 (MQ=255)
    tACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGt                                                                                                                                               <  1:21341/150‑1 (MQ=255)
    tACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGt                                                                                                                                               >  2:1185172/1‑150 (MQ=255)
     aCTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGAATAATTTCTGGCAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTg                                                                                                                                              <  2:116071/150‑1 (MQ=255)
      cTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGa                                                                                                                                             >  2:1164897/1‑150 (MQ=255)
       tGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGaa                                                                                                                                            >  1:1814198/1‑150 (MQ=255)
       tGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGttca                                                                                                                                            <  1:1358131/150‑4 (MQ=255)
        gggATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGTGGCGTGAAc                                                                                                                                           >  2:1382775/1‑150 (MQ=255)
         ggATAAGACCGGCGATGCGGTTGGGCCTAATATGATTAATTTCTGGAAGAATGGCAGTATGGCTGGCGCGTTCTTGCTATTGGCATTTACCGGGCCGGGGGCAATTTCTGTCGATCGGCGTTAGGAAAGATGCGGGATGCGGCGTGAACg                                                                                                                                          <  1:709017/150‑1 (MQ=255)
            tatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCt                                                                                                                                       <  1:1083346/150‑1 (MQ=255)
              tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                     >  2:536625/1‑150 (MQ=255)
               gACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                    >  1:384399/1‑150 (MQ=255)
               gACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                    >  2:332150/1‑150 (MQ=255)
                 ccGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                  >  1:1058672/1‑150 (MQ=255)
                   ggCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                >  1:478712/1‑150 (MQ=255)
                   ggCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                >  1:1346660/1‑150 (MQ=255)
                    gCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                               <  1:843056/150‑1 (MQ=255)
                      gATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                             <  1:1708790/150‑1 (MQ=255)
                      gATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                             >  2:1636450/1‑150 (MQ=255)
                        tGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAttt                                                                                                                           <  2:391119/150‑1 (MQ=255)
                        tGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAttt                                                                                                                           <  2:384410/150‑1 (MQ=255)
                        tGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAttt                                                                                                                           <  1:1163019/150‑1 (MQ=255)
                          cGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaa                                                                                                                         <  2:1577665/150‑1 (MQ=255)
                          cGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGGTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaa                                                                                                                         <  2:1584654/150‑1 (MQ=255)
                            gTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaata                                                                                                                       >  1:379942/1‑150 (MQ=255)
                              tGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATaaa                                                                                                                     <  1:1763893/150‑1 (MQ=255)
                                    aaaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAg                                                                                                               <  2:1345502/150‑1 (MQ=255)
                                    aaaTATGATTAATTTCTGGAAGAATGTCAGTAGCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAg                                                                                                               <  1:264069/150‑1 (MQ=255)
                                     aaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                              >  2:126311/1‑150 (MQ=255)
                                         tGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCAct                                                                                                          >  2:1421847/1‑150 (MQ=255)
                                          gATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTAAAGACaata                                                                                                         >  1:1367298/1‑147 (MQ=255)
                                           aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActct                                                                                                        >  1:198726/1‑150 (MQ=255)
                                           aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActct                                                                                                        >  1:1631029/1‑150 (MQ=255)
                                            ttAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGCTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTa                                                                                                       >  1:721114/1‑150 (MQ=255)
                                            ttAATTTCTGGAAGAATGTCAGAATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCGGGGGGCAATTTCTCTCGATAGGGGTTAGGAAAGATGCCGGATGCTGCGTGAAAGCCTTATACGGGATTTTTTTATTTACATCCActctt                                                                                                       >  1:1385283/1‑149 (MQ=255)
                                                   cTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCaa                                                                                                >  2:1009339/1‑150 (MQ=255)
                                                    tGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAc                                                                                               <  1:536625/150‑1 (MQ=255)
                                                     ggAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGCATGCGGCGTGAACGCCTTATCCGACATTTAATAAATTACAGCCACTCTCCCCGCAACg                                                                                              >  2:152038/1‑150 (MQ=255)
                                                     ggAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGTAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACg                                                                                              <  1:956065/150‑1 (MQ=255)
                                                     ggAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACg                                                                                              >  2:877207/1‑150 (MQ=255)
                                                          aaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTTCAGCCACTCTACCCGCAACGACAta                                                                                         >  2:707669/1‑149 (MQ=255)
                                                           aTGGCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGTAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtct                                                                                        <  2:1114792/150‑1 (MQ=255)
                                                            tGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtctc                                                                                       <  2:1021079/150‑1 (MQ=255)
                                                               cAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCGGa                                                                                    >  2:1163991/1‑150 (MQ=255)
                                                                   aTCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCTCAACGACATCTCTGAAgtt                                                                                >  2:1387513/1‑150 (MQ=255)
                                                                    tCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttg                                                                               >  1:678740/1‑150 (MQ=255)
                                                                      gCTGGCTCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATAGGCGTTAGGAAAGATGCCGGATGAGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGTAgttgtt                                                                             <  1:108491/150‑1 (MQ=255)
                                                                       cTGGCGCGGGCTTGCGATGGGCAATTACCGGGCGGGGGGCAATTTCTCTCGATGGGCGTTAGGAAAGATGCCGGATGCGGCGGGAACGCCTTATCCGGCATTTAATAAATTAGAGCGTCTCTAGCCGCAACGAGATCTCTGAAGttgttg                                                                            <  1:1215867/150‑1 (MQ=255)
                                                                         ggCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAt                                                                          >  2:1506584/1‑150 (MQ=255)
                                                                         ggCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAt                                                                          >  1:1039593/1‑150 (MQ=255)
                                                                                 cTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTgg                                                                  <  2:853550/150‑1 (MQ=255)
                                                                                  ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCACTgtt                                                                 >  2:1145000/1‑148 (MQ=255)
                                                                                   tGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg                                                                >  2:1521429/1‑150 (MQ=255)
                                                                                   tGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg                                                                >  2:1046347/1‑150 (MQ=255)
                                                                                   tGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg                                                                >  1:1669783/1‑150 (MQ=255)
                                                                                        ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCTCTCGACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc                                                           >  2:79934/1‑150 (MQ=255)
                                                                                        ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc                                                           >  2:1779144/1‑150 (MQ=255)
                                                                                        ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc                                                           >  2:1772373/1‑150 (MQ=255)
                                                                                         tGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcg                                                          >  1:1634766/1‑150 (MQ=255)
                                                                                          ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc                                                         >  1:659200/1‑150 (MQ=255)
                                                                                           gCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCa                                                        <  2:1814198/150‑1 (MQ=255)
                                                                                            cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCaa                                                       >  1:29900/1‑150 (MQ=255)
                                                                                            cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAGTTAATAAATTACCGCCACTCTACCCGCAACGACATCTCTGCAGTTGTTGATTCCCCTGGTGCTAGCGCaa                                                       >  2:469689/1‑150 (MQ=255)
                                                                                             aaTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt                                                      >  1:800808/1‑150 (MQ=255)
                                                                                             aaTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt                                                      <  2:227940/150‑1 (MQ=255)
                                                                                                tACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATgag                                                   >  1:384299/1‑150 (MQ=255)
                                                                                                 aCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGTCGGATGCGGCGTGAACTCCTTATCCGGAATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATgaga                                                  <  1:331535/150‑1 (MQ=255)
                                                                                                 aCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATgaga                                                  <  1:826448/150‑1 (MQ=255)
                                                                                                     ggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAc                                              <  1:1534487/150‑1 (MQ=255)
                                                                                                      gccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGTGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGTAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAcc                                             <  1:1382775/150‑1 (MQ=255)
                                                                                                       ccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAccc                                            <  1:1571932/150‑1 (MQ=255)
                                                                                                            ggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTc                                       >  2:720613/1‑150 (MQ=255)
                                                                                                             gCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTcc                                      <  1:1185172/150‑1 (MQ=255)
                                                                                                                aTTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCggg                                   <  2:1385674/150‑1 (MQ=255)
                                                                                                                    ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                               <  2:789049/150‑1 (MQ=255)
                                                                                                                    ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                               <  1:332150/150‑1 (MQ=255)
                                                                                                                     tctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatg                              <  1:1164897/150‑1 (MQ=255)
                                                                                                                      ctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatga                             >  2:50521/1‑150 (MQ=255)
                                                                                                                       tcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgat                            >  1:1684424/1‑150 (MQ=255)
                                                                                                                       tcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAACTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgat                            >  1:1592471/1‑150 (MQ=255)
                                                                                                                        ctaTCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGACTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTTAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                           <  1:877269/148‑1 (MQ=255)
                                                                                                                          aTCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatgat                         >  1:1019963/1‑150 (MQ=255)
                                                                                                                                   aGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcg                <  1:1421847/150‑1 (MQ=255)
                                                                                                                                   aGGAAAGATGCCGGATGCGGCGTGAACGCCTTATACGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCATGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATAATCCGgcg                >  2:1569651/1‑150 (MQ=255)
                                                                                                                                    ggAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCGACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgagc               >  1:236211/1‑150 (MQ=255)
                                                                                                                                     gAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCAACCCCCAACGAACTCTCTGAAGTTGTTGATTACCCAGGTGCTAGCGCAATGAGATCACCCCCATACGGGCGATGATTAACATACGgccaa              >  2:1557422/1‑147 (MQ=255)
                                                                                                                                         gATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGc          <  1:431055/150‑1 (MQ=255)
                                                                                                                                          aTGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCt         <  1:1135394/150‑1 (MQ=255)
                                                                                                                                          aTGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCt         <  2:380053/150‑1 (MQ=255)
                                                                                                                                          aTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGCCATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGAGGAGCATCCGGCGCATGGCt         >  2:722901/1‑150 (MQ=255)
                                                                                                                                          aTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCt         >  1:1579548/1‑150 (MQ=255)
                                                                                                                                           tGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCCCTCTACCCGCAACGACTTCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTc        >  1:1278553/1‑150 (MQ=255)
                                                                                                                                           tGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTc        >  2:1024199/1‑150 (MQ=255)
                                                                                                                                            gCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCa       >  2:1076390/1‑150 (MQ=255)
                                                                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCa       >  2:800021/1‑150 (MQ=255)
                                                                                                                                             ccGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAt      >  2:1494446/1‑150 (MQ=255)
                                                                                                                                             ccGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGCCATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAt      >  1:467140/1‑150 (MQ=255)
                                                                                                                                             ccGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAt      >  2:549850/1‑150 (MQ=255)
                                                                                                                                                 aTGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGt  <  1:1521429/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGT  >  NC_000913/2673636‑2673930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: