Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,704,308 C→T 5.6% intergenic (‑245/+27) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,704,3080CT 5.6% 405.2 / 7.9 126intergenic (‑245/+27)rnc/lepBRNase III/leader peptidase (signal peptidase I)
Reads supporting (aligned to +/- strand):  ref base C (58/60);  new base T (3/4);  total (61/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

Only 100 of 126 total aligned reads displayed.

GGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGC  >  NC_000913/2704160‑2704453
                                                                                                                                                    |                                                                                                                                                 
ggTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACg                                                                                                                                                  >  2:400763/1‑150 (MQ=255)
  tataTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTTCCAACCTTAAACTTCGGTGGAATAATATATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACTTTGTCGCCATAACGGc                                                                                                                                                <  1:44180/150‑1 (MQ=255)
  tataTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGc                                                                                                                                                <  1:810875/150‑1 (MQ=255)
   atatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCg                                                                                                                                               <  1:1352040/150‑1 (MQ=255)
   atatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCg                                                                                                                                               <  2:738460/150‑1 (MQ=255)
    tatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGa                                                                                                                                              <  1:802901/150‑1 (MQ=255)
        gggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAAc                                                                                                                                          <  2:1008896/150‑1 (MQ=255)
         ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGTAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACg                                                                                                                                         <  2:900454/150‑1 (MQ=255)
         ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAACg                                                                                                                                         <  2:127874/150‑1 (MQ=255)
         ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACg                                                                                                                                         >  2:546986/1‑150 (MQ=255)
         ggACCAACGCTGTTTCGTGTGCAGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAACg                                                                                                                                         <  1:1001522/150‑1 (MQ=255)
          gACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTTTCGCCATAACGGCGACAACGt                                                                                                                                        >  1:156969/1‑150 (MQ=255)
          gACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAACGt                                                                                                                                        <  2:1352165/150‑1 (MQ=255)
            ccAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGTGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGa                                                                                                                                      <  1:642160/150‑1 (MQ=255)
            ccAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGa                                                                                                                                      <  2:1001617/150‑1 (MQ=255)
            ccAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGa                                                                                                                                      >  1:764265/1‑150 (MQ=255)
              aaCGCTGTTTCTTGTGCAGTTGCAACCTGGAGTTGCCAACCTTAAACTTCTGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                    <  2:1416564/150‑1 (MQ=255)
              aaCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                    >  2:159926/1‑150 (MQ=255)
                 gCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCAATAACGGCGACAACGTGAACGaa                                                                                                                                 >  1:888542/1‑150 (MQ=255)
                  gtGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAg                                                                                                                                <  2:412735/149‑1 (MQ=255)
                     tttCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATg                                                                                                                             <  1:1099254/150‑1 (MQ=255)
                     tttAGTGTGCCGTGGATAGCTGGAGGTGCTAAAATTAAACTTCTGGGTAATTTTCTATACACAAGGACGGGGGGTGTCGTTAGCCACGGGAGATTTATATCATAAATAATTCACGTTGTCGCAATAACGGCGACAACGTGAACGAAGATg                                                                                                                             <  2:703430/150‑1 (MQ=255)
                       tCGTGTGCCGTGGCAACCTGGAGGTGACAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGc                                                                                                                           >  2:1187662/1‑150 (MQ=255)
                        cGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCt                                                                                                                          >  2:1756881/1‑150 (MQ=255)
                         gtgtGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTa                                                                                                                         >  2:1031357/1‑150 (MQ=255)
                          tgtgCCGTGGCAACCTGCAGGTGCCAACCTAAAACTTCGGGGGAATATTCTATACACAACGCCGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTAt                                                                                                                        <  1:1401256/150‑1 (MQ=255)
                           gtgCCGTGGCAACCTGGAGGTGCCAACCGTAAACTTCGGGGGAATAATCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTAtt                                                                                                                       <  1:1225306/150‑1 (MQ=255)
                             gCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTaa                                                                                                                     >  2:46376/1‑150 (MQ=255)
                             gCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTaa                                                                                                                     >  2:828932/1‑150 (MQ=255)
                              ccGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAAt                                                                                                                    >  1:1341260/1‑150 (MQ=255)
                               cGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                   <  2:160636/150‑1 (MQ=255)
                                gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATgg                                                                                                                  >  1:1774023/1‑150 (MQ=255)
                                 tGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGATTTTTCTATACACAACGCCGGGGGATGTCGTTAGCCCCGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAGCGAAGATGGCTATTAATggt                                                                                                                 >  1:235243/1‑149 (MQ=255)
                                  ggCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGAt                                                                                                                >  1:1340098/1‑150 (MQ=255)
                                    cAACCTGGAGGTGCCAACCTTTAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgc                                                                                                              >  1:358259/1‑150 (MQ=255)
                                    cAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgc                                                                                                              >  2:971676/1‑150 (MQ=255)
                                    cAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgc                                                                                                              >  2:1252894/1‑150 (MQ=255)
                                    cAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgc                                                                                                              >  1:1738941/1‑150 (MQ=255)
                                           gAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGGCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAAt                                                                                                       <  1:370778/150‑1 (MQ=255)
                                            aGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATg                                                                                                      <  1:1294544/150‑1 (MQ=255)
                                                gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATTGCTATTAATGGATGCCGCCAATGCGAc                                                                                                  >  1:862800/1‑150 (MQ=255)
                                                gCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGAc                                                                                                  <  1:1443620/150‑1 (MQ=255)
                                                     ccTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAg                                                                                             >  2:1061750/1‑150 (MQ=255)
                                                        tAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgcgc                                                                                          <  1:878829/150‑1 (MQ=255)
                                                         aaaCTTCTGGGGAATATTCTAAACACAACGACGGGGGATGTCGTTATCCACGGGAGATTTATCTCATAAATAATTCACGTTTTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCa                                                                                         <  1:580132/150‑1 (MQ=255)
                                                         aaaCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCa                                                                                         >  1:19019/1‑150 (MQ=255)
                                                             ttCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGAcc                                                                                     >  1:413490/1‑150 (MQ=255)
                                                             ttCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGAcc                                                                                     <  2:69451/150‑1 (MQ=255)
                                                               cGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAg                                                                                   >  2:1402107/1‑150 (MQ=255)
                                                                  gggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCg                                                                                <  2:1274945/150‑1 (MQ=255)
                                                                    gAATAATCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGc                                                                              <  1:938390/150‑1 (MQ=255)
                                                                     aaTATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGcc                                                                             <  1:1651000/150‑1 (MQ=255)
                                                                       tatTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAt                                                                           >  1:231191/1‑150 (MQ=255)
                                                                            tataCACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGcc                                                                      >  2:471806/1‑150 (MQ=255)
                                                                                  caACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGCCAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTg                                                                >  2:1332586/1‑150 (MQ=255)
                                                                                  caACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTg                                                                >  2:1124522/1‑150 (MQ=255)
                                                                                  caACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTg                                                                >  1:1122678/1‑150 (MQ=255)
                                                                                  caACGACGGGGGATGTAGTTAGCCACGGGAGATTTATATCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGGGAACGAAGATGGCTATTAATGGATGCAGCCAATGCGACTTAAGCGCAGAACAGTCGGCCATTCGCCTACTTg                                                                >  1:120660/1‑150 (MQ=255)
                                                                                   aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTATGCGCAGACCAGTCGGCCATTCGCCTTCTTGc                                                               <  2:348552/150‑1 (MQ=255)
                                                                                    ccgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCt                                                              <  1:401022/149‑1 (MQ=255)
                                                                                    acgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCt                                                              <  1:601456/150‑1 (MQ=255)
                                                                                     cgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCtt                                                             >  1:475304/1‑150 (MQ=255)
                                                                                        cgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                          <  1:659195/150‑1 (MQ=255)
                                                                                        cgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                          <  1:504416/150‑1 (MQ=255)
                                                                                        cgGGGGATGTCGTTAGCCACGGGAGATTAATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                          <  2:1576357/150‑1 (MQ=255)
                                                                                        cgGGGGAGGTCGTTAGCCACGGGAGAATTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCTCCAATGCGACTTAAGCGCAGACCAGTCGGCAATTCGCCTTCTTGCTTATc                                                          <  2:838438/150‑1 (MQ=255)
                                                                                          ggggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGa                                                        <  1:818954/150‑1 (MQ=255)
                                                                                          ggggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATAAATGGATGCCTCCTATGCGACTTAAGCGCAGACCAGTCGGCCATTCGGCTTCTTGCTTATcta                                                        >  2:282283/1‑148 (MQ=255)
                                                                                             gATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGc                                                     <  1:189843/150‑1 (MQ=255)
                                                                                              aTGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCAACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGcg                                                    >  1:612327/1‑149 (MQ=255)
                                                                                                gTCGTTAGCCACGGGATATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCa                                                  <  1:493246/150‑1 (MQ=255)
                                                                                                    ttAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCa                                              <  2:688708/150‑1 (MQ=255)
                                                                                                    ttAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCa                                              <  2:1774023/150‑1 (MQ=255)
                                                                                                       gCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGAt                                           >  2:1301396/1‑150 (MQ=255)
                                                                                                        ccACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATa                                          <  2:1392319/150‑1 (MQ=255)
                                                                                                        ccACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTAATCCAGATa                                          >  2:1425897/1‑150 (MQ=255)
                                                                                                         cACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAg                                         >  1:389220/1‑150 (MQ=255)
                                                                                                          aCGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGc                                        <  1:623322/150‑1 (MQ=255)
                                                                                                          aCGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGc                                        <  1:1521968/150‑1 (MQ=255)
                                                                                                           cGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGcc                                       >  1:520391/1‑150 (MQ=255)
                                                                                                                 aTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGcc                                 <  2:201957/150‑1 (MQ=255)
                                                                                                                   ttATCTCATAAATAATTCACGTTGTCGCCATAACGGCGAGAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccg                               <  2:799358/150‑1 (MQ=255)
                                                                                                                   ttATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAATATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccg                               <  2:86734/150‑1 (MQ=255)
                                                                                                                   ttATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccg                               <  1:1002768/150‑1 (MQ=255)
                                                                                                                      tctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgacc                            <  1:1085272/150‑1 (MQ=255)
                                                                                                                       ctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccg                           >  1:215420/1‑150 (MQ=255)
                                                                                                                         cATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                         >  2:1804476/1‑150 (MQ=255)
                                                                                                                         aataaataaTTCACGTTGTCGCCATAAAGGCGAAAACGTGAACGAAGATGGCTATTAATGGTTGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                         <  2:1329499/149‑1 (MQ=255)
                                                                                                                          ataaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCgaccgacc                        <  2:1114336/150‑1 (MQ=255)
                                                                                                                                aatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGACATTCGCCTTCTTGCTAATCGAAGCTCATCCAGATAGCCGTTGCCCGCCCGACCAGATTc                  >  2:527812/1‑150 (MQ=255)
                                                                                                                                 atTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCg                 <  1:1556695/150‑1 (MQ=255)
                                                                                                                                 atTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCGGATCCAGATAGCCGGTGCCAGACCGCCCAGCTTCg                 >  1:1542971/1‑150 (MQ=255)
                                                                                                                                 atTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATGCGCCGGCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCg                 <  1:489175/150‑1 (MQ=255)
                                                                                                                                    cACGTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATAAATGGATCCCGCCATTGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTATTGCTTATCGAAGCTCATCCAGATAGCGGATGCACGACCGAACAGATTCGAtt              >  1:250195/1‑150 (MQ=255)
                                                                                                                                     aCGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTc             <  2:913689/150‑1 (MQ=255)
                                                                                                                                      cGTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGGCTTAAGCGCAGACCAGTCGGCCAGTCGCCTGGTTGCTTATCGAAGCTCATCCAGATAGCCGTTGGCCGGCCGGCCAGATTCGCGTcc            >  1:294031/1‑150 (MQ=255)
                                                                                                                                      cGTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTcc            >  1:1235543/1‑150 (MQ=255)
                                                                                                                                       gTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCg           <  2:1122678/150‑1 (MQ=255)
                                                                                                                                            cGCCATAACGGCGCCAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGcacc      >  1:1718703/1‑149 (MQ=255)
                                                                                                                                                aTAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGc  >  1:250504/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGC  >  NC_000913/2704160‑2704453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: