BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,724,413	A→T	5.3%	S96C (AGC→TGC)	yfiM →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,413	0	A	T	5.3%	327.2 / 5.4	111	S96C (AGC→TGC)	yfiM	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base A (59/46); new base T (3/3); total (62/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 113 total aligned reads displayed.

ACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGC  >  NC_000913/2724265‑2724562

acaGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAg                                                                                                                                                      >  2:1748426/1‑150 (MQ=255)

 caGCATCAGGGGATGAGCCGGGATCGCAGTGCCAAGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGc                                                                                                                                                     >  2:1474952/1‑150 (MQ=255)

       cAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCTTGCACCGGc                                                                                                                                               <  2:1521077/150‑1 (MQ=255)

        cggggATGAGCCGGGATCGCAGCGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCTTGCACCGGcg                                                                                                                                              <  1:1433185/149‑2 (MQ=255)

          gggATGAGCCGGGATCGCAGTGCCATGTTTGGATTGCTGTGCTCTGTCAGTTTGGGGGCGTCAAACGAGCTTGGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCTTGCACCGGcgac                                                                                                                                            <  1:565885/150‑4 (MQ=255)

            gATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATAc                                                                                                                                          >  2:1281917/1‑150 (MQ=255)

              tGAGCCGGGATCGCAGTTCCATGTTGGGAGTGATGTTCGCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCg                                                                                                                                        <  2:84732/150‑1 (MQ=255)

              tGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCg                                                                                                                                        >  2:258707/1‑150 (MQ=255)

                aGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAATAGCTTTGGGATAGCCGCCCCGAAGGGAGCGCCTGGAGCTGGAAGGATTTGGCCAGGGATGTCGCCGGTGCAAGCACAGGCTTTACCGta                                                                                                                                      >  2:545639/1‑149 (MQ=255)

                aGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTc                                                                                                                                      >  2:577182/1‑150 (MQ=255)

                  ccGGGATCGCCGTGCCATGTTTGGATTGAGGTTCTCTGTGAGTGTGGGGGCGGCAAAAGAGCTTTGGGATAGCCGCCCCGTAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGTATGTCGCCGGTGCAAGCACCGGCTATACCGTCTg                                                                                                                                    <  1:960371/150‑1 (MQ=255)

                  ccGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTg                                                                                                                                    <  2:1796034/150‑1 (MQ=255)

                       aTCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAAc                                                                                                                               <  1:1294163/150‑1 (MQ=255)

                       aTCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTAGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCCGCTATACCGTCTGGCAAc                                                                                                                               >  1:1775195/1‑150 (MQ=255)

                         cGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTg                                                                                                                             >  1:685137/1‑150 (MQ=255)

                           cAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCG‑CCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGAcc                                                                                                                          >  2:1753901/1‑150 (MQ=255)

                            aGTGCCAAGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGAcc                                                                                                                          <  1:1474952/150‑1 (MQ=255)

                                          aTTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACg                                                                                                            >  1:751277/1‑150 (MQ=255)

                                          aTTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGACGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACg                                                                                                            <  2:1472857/150‑1 (MQ=255)

                                           ttGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGc                                                                                                           >  2:35370/1‑150 (MQ=255)

                                                gTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATccc                                                                                                      <  2:1163948/150‑1 (MQ=255)

                                                 ttCTCTGTCAGTTTGGGGGCGGCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGCCACTAAAGACGCATcccc                                                                                                     <  1:207359/150‑1 (MQ=255)

                                                   ctctGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCccctt                                                                                                   >  1:385437/1‑150 (MQ=255)

                                                      tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCcccttccc                                                                                                >  1:1580085/1‑150 (MQ=255)

                                                       gTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCcccttccct                                                                                               >  2:581620/1‑150 (MQ=255)

                                                        tCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGTAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCcccttccctt                                                                                              >  1:1475895/1‑150 (MQ=255)

                                                        tCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTAGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCCACTGACCCGTCACTAAAGACGCATCccctgccctt                                                                                              >  2:481990/1‑150 (MQ=255)

                                                            tttGGGGGCGTCAACAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCg                                                                                          <  1:1177579/150‑1 (MQ=255)

                                                            tttGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCg                                                                                          <  1:12084/150‑1 (MQ=255)

                                                            gttGGGGGCGTCAACAGAGCTTTGGGTTAGACGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACACGTCACTAAAGACGCATCCCCTTCCCTTTGCg                                                                                          <  1:919036/149‑1 (MQ=255)

                                                             ttGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGa                                                                                         >  2:660226/1‑150 (MQ=255)

                                                              tGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGAt                                                                                        >  1:792392/1‑150 (MQ=255)

                                                              ggggggCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATGTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGAt                                                                                        <  1:166468/149‑1 (MQ=255)

                                                                  ggCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAg                                                                                    <  1:1222829/150‑1 (MQ=255)

                                                                    cGTCAAAAGAGCTTTGGGATATCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCAGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAgaa                                                                                  >  2:665477/1‑148 (MQ=255)

                                                                    cGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCa                                                                                  <  1:906310/150‑1 (MQ=255)

                                                                     gTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGCCCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCAt                                                                                 >  1:1318933/1‑150 (MQ=255)

                                                                      tCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATc                                                                                >  2:1025277/1‑150 (MQ=255)

                                                                          aaGTGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAg                                                                            <  1:547772/150‑1 (MQ=255)

                                                                          aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCCCCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCAGCCCCTTCCCTTTGCGATGTAGCATCAAAg                                                                            >  2:934172/1‑150 (MQ=255)

                                                                             cgCTTTGCGCTAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGACGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGaaa                                                                         <  1:1172403/149‑1 (MQ=255)

                                                                              gcgttGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAAc                                                                        <  2:321273/147‑1 (MQ=255)

                                                                               cTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAAcc                                                                       >  2:419621/1‑150 (MQ=255)

                                                                               cTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAAcc                                                                       >  1:421110/1‑150 (MQ=255)

                                                                                 ttGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAg                                                                     <  1:1781855/150‑1 (MQ=255)

                                                                                 ttGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGAGGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAg                                                                     <  2:1719130/150‑1 (MQ=255)

                                                                                   gggATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaa                                                                   >  2:739565/1‑150 (MQ=255)

                                                                                    ggATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCCAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGCCGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaac                                                                  >  1:1602263/1‑149 (MQ=255)

                                                                                     gATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCGTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAAc                                                                 >  1:1369661/1‑150 (MQ=255)

                                                                                      aTAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGCCCCGTCACTACAGCCGCATCCCCTTCCCTTTGCGCTGGAGCATCAATGACCCCAGAAACg                                                                >  1:1103934/1‑150 (MQ=255)

                                                                                        aGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGcc                                                              >  2:535692/1‑150 (MQ=255)

                                                                                         gccgccCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccc                                                             >  1:1404571/1‑149 (MQ=255)

                                                                                          ccgccCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccac                                                            <  1:1458785/150‑1 (MQ=255)

                                                                                             ccccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCcaccac                                                         <  1:1036232/150‑1 (MQ=255)

                                                                                              cccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAATACGCATCCCCGTCCCTTTGCGAAGTAGCAGCATAGAAACCAGAAACCCCACCCCg                                                        >  1:97383/1‑150 (MQ=255)

                                                                                               ccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGCAACCAGAAACGCCACCACgg                                                       >  1:82676/1‑150 (MQ=255)

                                                                                               ccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACgg                                                       >  2:1005299/1‑150 (MQ=255)

                                                                                                  aaGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCa                                                    <  2:1468811/150‑1 (MQ=255)

                                                                                                  aaGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCa                                                    >  2:1676648/1‑150 (MQ=255)

                                                                                                    gggAGCGGCTGGATCGGGAAGGATTTGGCCTGGTATGTCGCCGGTGCAAGCACCGGCTATACAGTCTGGAAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCGAAGAAACCAGAAACGCCACCACGGCCATc                                                  <  1:1146952/150‑1 (MQ=255)

                                                                                                    gggAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATc                                                  <  1:707774/150‑1 (MQ=255)

                                                                                                     ggAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCa                                                 <  2:641511/150‑1 (MQ=255)

                                                                                                     ggAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCa                                                 >  2:1409822/1‑150 (MQ=255)

                                                                                                        gCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAgg                                              <  2:1791706/150‑1 (MQ=255)

                                                                                                          ggCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTc                                            >  2:856883/1‑150 (MQ=255)

                                                                                                                 gctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATAcc                                     <  2:1761076/150‑1 (MQ=255)

                                                                                                                   tggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCag                                   <  2:815544/150‑1 (MQ=255)

                                                                                                                   tggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCag                                   >  2:522380/1‑150 (MQ=255)

                                                                                                                    ggaaggaaTTGGCCTGGGAGGTCGCCGGTGCAAGCACCGGCGATACCGTCTGGCTCCTGACCCGTCACTAAAGACGCATCCCCTGCCCTTTGCGATGTAGCATCAAAGCAACCAGAAACGCCACCACGGCCATCAAGGTCAGCTACCagt                                  >  2:319828/1‑149 (MQ=255)

                                                                                                                    ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAAAGCCCCCACGGCCATCAAGGTCACATACCaga                                  >  1:1625558/1‑150 (MQ=255)

                                                                                                                    ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCAACCCCTTCCCTTTGCGATGTAGCATCAAACAAACCAGAAACGCCACCACCCGCAACAAGGTCACAAACCcga                                  >  1:1668154/1‑150 (MQ=255)

                                                                                                                    ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGACACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCaga                                  >  2:1548238/1‑150 (MQ=255)

                                                                                                                     gaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCagaa                                 >  2:576462/1‑150 (MQ=255)

                                                                                                                         gATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGaagaag                             <  1:1748426/150‑1 (MQ=255)

                                                                                                                           tttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAGCCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGc                           >  1:1007594/1‑150 (MQ=255)

                                                                                                                           tttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGc                           <  1:1676648/150‑1 (MQ=255)

                                                                                                                             tGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATTCCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAGCGCCACCACGGCCATCAAGGTCACATCCCAGAAGACGGGTg                         >  1:1087181/1‑150 (MQ=255)

                                                                                                                               gCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGAAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGtt                       <  1:114448/150‑1 (MQ=255)

                                                                                                                               gCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGAGGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATCCCAGAAGAAGGCTGtt                       >  1:268669/1‑150 (MQ=255)

                                                                                                                                ccTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGttt                      >  2:928622/1‑150 (MQ=255)

                                                                                                                                 cTGGGATGTCGCCTGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTc                     <  2:1311280/150‑1 (MQ=255)

                                                                                                                                    ggATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCCCCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCAt                  >  1:1431262/1‑150 (MQ=255)

                                                                                                                                     gATGTCGCCGGTGCAAGAACCGGCTATACCGTCTGGCAACTGACCGGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAGGAAGGCTGTTTCCAtt                 <  2:1632562/150‑1 (MQ=255)

                                                                                                                                     gATGGCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCAtt                 <  1:1673919/150‑1 (MQ=255)

                                                                                                                                      gtatagCCGGTGCTTGCACCGGCTATCCCGTCTGGCAACTGCCCCGTCCCTAAAGACGCCTCCCCTTCCCTTTGCGATGGAGCATCAAAGAAACCAGAAACGCCCCCACGGCCATCAAGGTCACCTACCAGAAGAAGGCTGTTTCCATTc                >  1:1488494/6‑150 (MQ=255)

                                                                                                                                       tGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTcc               <  2:385437/150‑1 (MQ=255)

                                                                                                                                       tGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTcc               <  1:739565/150‑1 (MQ=255)

                                                                                                                                       tGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTcc               <  1:1281917/150‑1 (MQ=255)

                                                                                                                                          cGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAACCGCCCCCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt            >  1:701704/1‑150 (MQ=255)

                                                                                                                                          cGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt            >  1:865583/1‑150 (MQ=255)

                                                                                                                                          agCCGGTGCTTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGTAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt            >  2:1406938/2‑150 (MQ=255)

                                                                                                                                          agCCGGTGATTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACAAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGCCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt            >  1:1314698/2‑150 (MQ=255)

                                                                                                                                            ccGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTg          >  1:31481/1‑150 (MQ=255)

                                                                                                                                            ccGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTg          <  1:258707/150‑1 (MQ=255)

                                                                                                                                             cGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGa         <  1:673745/150‑1 (MQ=255)

                                                                                                                                                 gCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTc     <  1:1781048/150‑1 (MQ=255)

                                                                                                                                                  cAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTCGCATCAATGACACCAGAAACGCCACCACGGCCCGCAAGGGCGCAGACAAGAAGATGGCGGTTTGCATTCCTATGGATTTcg    >  2:894481/1‑149 (MQ=255)

                                                                                                                                                  cAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTATGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCa    >  1:1411011/1‑150 (MQ=255)

                                                                                                                                                    aGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGc  >  1:1531716/1‑150 (MQ=255)

                                                                                                                                                    aGCACCGGCTATACCGTATTGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATTTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATAACATAAGAAGGCTGTTTCCATTCCTATTGATTTCAGc  <  2:1107848/150‑1 (MQ=255)

ACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGC  >  NC_000913/2724265‑2724562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`