Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,837 T→A 5.1% intergenic (‑91/+232) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,8370TA 5.1% 374.4 / 4.0 120intergenic (‑91/+232)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (60/53);  new base A (2/5);  total (62/58)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATA  >  NC_000913/2725688‑2725986
                                                                                                                                                     |                                                                                                                                                     
cggcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTGATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCatat                                                                                                                                                       >  2:685443/1‑150 (MQ=255)
     acgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTa                                                                                                                                                  <  1:453681/150‑1 (MQ=255)
      cgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCTTAATCAGATGCTTTTGTCTGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTTAATATCATTaa                                                                                                                                                 >  2:631359/1‑149 (MQ=255)
           aTCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTATTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAc                                                                                                                                            <  1:349188/150‑1 (MQ=255)
            tctgtactTGTCAGTTTGCTGGCTGCCGTTACAGTACTTTCAGCCATGCCATTATTTCTCCTTCTGTTATGGGTTGCTTATGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGATCTCTTCTACATTTGTTGCATATCATTATGCAAcc                                                                                                                                           <  2:279182/146‑1 (MQ=255)
            tctctactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTTATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATAATGATATGCAAca                                                                                                                                           <  2:1242812/147‑2 (MQ=255)
            tCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTTTTGCATATAATTATGCAAcc                                                                                                                                           >  1:1527175/1‑150 (MQ=255)
             cACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTTAAGGAAATATTATACATTTGTTGCATATCTTTATGCAACCt                                                                                                                                          >  2:355292/1‑150 (MQ=255)
              actactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATAATGATATGCAAcaaa                                                                                                                                         <  1:215159/150‑4 (MQ=255)
                tactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATAATGATATGCAAcaaatg                                                                                                                                       <  2:1616873/150‑6 (MQ=255)
                 cctTGTCAGTTTGCTGTCTGCCGGTACCGTTCTTTCAGCCATGCCATTATGTCTCCTGCCGTACTCCGATGCTTTTGTCGGTCGCTTTTGGTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATAATGATATGCAAcaaatgt                                                                                                                                      <  2:1778705/149‑7 (MQ=255)
                 actTGTCAGTTTGCTGTCTGCCGGTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAATTATTTTTCATTTGTTTCATAAAATTTTGGAACCTTaaa                                                                                                                                      >  1:703711/1‑149 (MQ=255)
                  ctagTCAGTTTGCTGTCTGCAGTTAAAGTACTTTCAGACATGACATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGCATATCATTATGAAACCTTAAcc                                                                                                                                     >  2:728602/4‑150 (MQ=255)
                  ctTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAcc                                                                                                                                     <  1:839001/150‑1 (MQ=255)
                  ctTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAcc                                                                                                                                     <  2:1509923/150‑1 (MQ=255)
                    tGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCAt                                                                                                                                   >  1:902622/1‑150 (MQ=255)
                     gTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTTTTTTTTTTGTTAAGGAAATATTTTTCAATTGTTGGATATCATTATGCAAACATAAACaat                                                                                                                                  >  1:763127/1‑148 (MQ=255)
                        aGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAt                                                                                                                               <  1:1757915/150‑1 (MQ=255)
                        aGTTTGCTGTCTGCCGTTACAGTAATTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGGAACCTTAACCATGAAtt                                                                                                                              >  1:360425/1‑150 (MQ=255)
                        aGTATGCTGTATGCCGTTACAGTACTATCAGCAATGACATTATGTCTCATGCTGTAATACTATGATTTATTCTGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAt                                                                                                                               <  1:294043/150‑1 (MQ=255)
                         gTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCTGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAtt                                                                                                                              <  2:586042/150‑1 (MQ=255)
                         gTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAtt                                                                                                                              >  2:430703/1‑150 (MQ=255)
                         gTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAAACCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAtt                                                                                                                              <  2:1339123/150‑1 (MQ=255)
                          tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAttt                                                                                                                             <  2:1389104/150‑1 (MQ=255)
                           ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                            <  1:557237/150‑1 (MQ=255)
                           ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGACGTATTACGATGCTTTTGTCGGTGGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCAAATCATTATGCAACCTTAACAATGAATtta                                                                                                                            >  1:43250/1‑150 (MQ=255)
                              cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagtt                                                                                                                         <  2:1666489/150‑1 (MQ=255)
                                 tCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGc                                                                                                                      <  2:605084/150‑1 (MQ=255)
                                 tCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGc                                                                                                                      <  2:1770258/150‑1 (MQ=255)
                                 tCTGCCGTTAAAGTAATTTCTTGCATGCCATTATGTCTCCTGTCGTATTCCGTTGCTTTTGTGGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGTAACCTTAACCATGAATTTAGTTAGc                                                                                                                      <  2:716004/150‑1 (MQ=255)
                                   tGCCGTTACAGTTCTTTCAGCCATGCAATTATGTCTACTTCCGTAATCCGATTCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTTTGCAACCTTAACCATGAATTTAGTTAGCAg                                                                                                                    <  1:96728/150‑1 (MQ=255)
                                   tGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATCATTTTGCAACATTAACCATGAatttattttgaag                                                                                                                    >  1:415781/1‑145 (MQ=255)
                                   tGCCGTTACAGTACTTTCAGCCAAGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAg                                                                                                                    <  2:373155/150‑1 (MQ=255)
                                    gCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAgg                                                                                                                   >  1:440256/1‑150 (MQ=255)
                                      cGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTTTTTTTTTTGTAAAAGAAATTTTTAAAATTTTTTTCATATAATTATTCAAAATTAAACATGAATTTAGTTAGAAGGaa                                                                                                                 >  2:816558/1‑150 (MQ=255)
                                       gTTACAGTTCTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGCATATCATTATTCAAACTTAACCATGAATTTATTTAGAAGGaaa                                                                                                                >  2:1320515/1‑150 (MQ=255)
                                          aCAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                                             <  1:534679/150‑1 (MQ=255)
                                           cAGTACTTTTATCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATACATTTGTTGCATATCATTATGCATCCTTAACCATGAATTTAGTTAGCAGGAAAAttg                                                                                                            <  1:127464/150‑3 (MQ=255)
                                           cAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATgg                                                                                                            <  2:392754/150‑1 (MQ=255)
                                           cAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATgg                                                                                                            >  2:1677833/1‑150 (MQ=255)
                                             ttACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTAGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGtt                                                                                                          <  1:1064426/149‑1 (MQ=255)
                                             gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATAATTATGAAAACTTAAACATTAATTTAGTTTGCATGAAAATTGtt                                                                                                          >  1:1428167/1‑150 (MQ=255)
                                               aCTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGCATTTCATTATGCAACCTTAACCATGAATTTATTTATCAGTAAAATGGtttt                                                                                                        >  1:923148/1‑148 (MQ=255)
                                                cTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCATGAAAATGGTTAtt                                                                                                       >  2:1001172/1‑150 (MQ=255)
                                                cTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTATTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATAATTATTCAACCTTAACCATGAATTTAGTTAGCATGAAAATGGTtttt                                                                                                       >  1:1374087/1‑150 (MQ=255)
                                                     aGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGCATATAATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATTGTTAtttatgt                                                                                                  >  2:1396936/1‑145 (MQ=255)
                                                      gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgaggag                                                                                                 >  1:1396939/1‑150 (MQ=255)
                                                          tgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTa                                                                                             >  1:275787/1‑150 (MQ=255)
                                                            ccatTATGTCTCCTGCCGTAATGCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATTCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGGTATTGAGGAGCTTAAg                                                                                           <  2:922787/150‑1 (MQ=255)
                                                            ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAg                                                                                           <  1:601726/150‑1 (MQ=255)
                                                                tATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATAATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATa                                                                                       >  1:800080/1‑150 (MQ=255)
                                                                tATGTCTCCTGCCGTAATCCGATGCTTTTGTAGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATa                                                                                       >  2:791904/1‑150 (MQ=255)
                                                                  tGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATaaa                                                                                     <  1:1306590/150‑1 (MQ=255)
                                                                    tctcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAAtt                                                                                   >  2:386190/1‑150 (MQ=255)
                                                                      tcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTc                                                                                 >  2:1614198/1‑150 (MQ=255)
                                                                      tcCTGCCGTAATCCGATGATTTTGACGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTAAACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTATCAGGAAAATGGTTATTTAGGAGCTTAAGGATAAAttta                                                                                 >  2:1671271/1‑149 (MQ=255)
                                                                       ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAAAATTATAAATTTGTTTCATATCATTATTCAACCTTAAACATGAATTTATTTATCAGGAAAATGGTTATTGAGGATCTTAAGGATAAAtttat                                                                                >  1:703822/1‑148 (MQ=255)
                                                                       ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAACATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                <  1:1794598/150‑1 (MQ=255)
                                                                       ccTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGCAAATATTATACATTTTTTGCATAACATTATGCCACCATAACCATTAAATTAGTTAGCAGGAAAATTGTTTTTGAGGAGGTTAAGGATAAAtttaa                                                                                >  1:1396490/1‑148 (MQ=255)
                                                                        cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATTGTTATTGAGTAGCTTAAGGATAAATTTCTg                                                                               >  1:1130740/1‑150 (MQ=255)
                                                                        cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGATGAGCTTAAGGATAAATTTCTg                                                                               >  1:962370/1‑150 (MQ=255)
                                                                        cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                               <  1:1319401/150‑1 (MQ=255)
                                                                        cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                               <  1:598717/150‑1 (MQ=255)
                                                                        cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                               <  1:1659574/150‑1 (MQ=255)
                                                                          gacGTAATCAGATGCTTTTGTAGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGt                                                                             <  2:296868/148‑1 (MQ=255)
                                                                            cGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTTCATATAATTATTCAACCTTAAACATTAATTTAGTTATCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTATGGTaa                                                                           >  1:380159/1‑150 (MQ=255)
                                                                              tAATCCGATTCTTTTGTCGGTCGCTTTTGTTTAATTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTCATTGAGGAGCTTAAGGATAAATTTCTGGTAagg                                                                         <  2:894786/150‑1 (MQ=255)
                                                                                      tGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAACTTAACAATGAATTTATTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACg                                                                 >  1:203519/1‑150 (MQ=255)
                                                                                         ttttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                              >  2:694403/1‑150 (MQ=255)
                                                                                                   cgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGc                                                    <  1:18545/150‑1 (MQ=255)
                                                                                                           tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                            <  1:1396936/150‑1 (MQ=255)
                                                                                                           tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                            <  1:1821470/150‑1 (MQ=255)
                                                                                                           tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                            <  1:1001172/150‑1 (MQ=255)
                                                                                                           tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTAAACCATGAATTTAGTTAGCAGGAAAATGGTAATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                            <  2:1697417/150‑1 (MQ=255)
                                                                                                             tATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGa                                          >  2:1107114/1‑150 (MQ=255)
                                                                                                               ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                                        >  2:1076329/1‑150 (MQ=255)
                                                                                                                tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                       >  2:1738482/1‑150 (MQ=255)
                                                                                                                 ttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGGAAGTTGGGGAAGcc                                      >  1:426116/1‑150 (MQ=255)
                                                                                                                   ttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGt                                    >  1:1527706/1‑150 (MQ=255)
                                                                                                                      tGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTAta                                 >  1:1502601/1‑149 (MQ=255)
                                                                                                                       gTAAAGGAAATATTATACATTTGTTGCATATCATTTTGCAACCTTAACAATGAATTTAGTTAGCAGGAAAATGGTTATTTAGGATCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTTGGCAAGTTGGGGAAGCCGTATcc                                >  1:84310/1‑150 (MQ=255)
                                                                                                                       gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc                                >  2:1366995/1‑150 (MQ=255)
                                                                                                                       gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc                                >  2:801916/1‑150 (MQ=255)
                                                                                                                       gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATca                                >  1:1647806/1‑149 (MQ=255)
                                                                                                                         aaaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGt                              >  2:1323665/1‑150 (MQ=255)
                                                                                                                         aaaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGt                              >  2:1026792/1‑150 (MQ=255)
                                                                                                                           aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg                            >  1:1535714/1‑150 (MQ=255)
                                                                                                                            ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                           <  2:813133/150‑1 (MQ=255)
                                                                                                                            ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                           <  1:816558/150‑1 (MQ=255)
                                                                                                                              aaaTATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTg                         >  1:1143873/1‑150 (MQ=255)
                                                                                                                                    tataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTg                   >  1:733636/1‑150 (MQ=255)
                                                                                                                                      taCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGc                 <  2:1412410/150‑1 (MQ=255)
                                                                                                                                        cATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCat               >  1:651358/1‑150 (MQ=255)
                                                                                                                                         aTTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCata              >  1:170773/1‑150 (MQ=255)
                                                                                                                                             gttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAtgtg          >  1:854160/1‑150 (MQ=255)
                                                                                                                                             gttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGCTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAtgtg          >  2:225169/1‑150 (MQ=255)
                                                                                                                                               tgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTggg        >  2:1497841/1‑150 (MQ=255)
                                                                                                                                                     tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtata  >  2:329519/1‑150 (MQ=255)
                                                                                                                                                     tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtata  >  1:786826/1‑150 (MQ=255)
                                                                                                                                                     tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtata  >  2:986727/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATA  >  NC_000913/2725688‑2725986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: