Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,744,282 G→T 5.2% S83R (AGC→AGA rplS ← 50S ribosomal subunit protein L19

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,744,2820GT 5.2% 420.2 / 7.8 133S83R (AGC→AGArplS50S ribosomal subunit protein L19
Reads supporting (aligned to +/- strand):  ref base G (65/60);  new base T (5/3);  total (70/63)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTC  >  NC_000913/2744133‑2744431
                                                                                                                                                      |                                                                                                                                                     
 ccagccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGc                                                                                                                                                      <  2:861510/150‑1 (MQ=255)
 ccagccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGc                                                                                                                                                      >  1:1318187/1‑150 (MQ=255)
   agccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTg                                                                                                                                                    <  2:723774/150‑1 (MQ=255)
    gccaATTGGCCAGCCCTTCTTTACAGGTTGTGGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGt                                                                                                                                                   <  2:640426/150‑1 (MQ=255)
         ttGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCGTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGCATTTCTGTCaaac                                                                                                                                              <  2:267617/150‑3 (MQ=255)
            gCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGCACAGCACCCCGACGTTTGACAGAAATGCTGTCAACTAcc                                                                                                                                           >  1:1183161/1‑150 (MQ=255)
             ccAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCg                                                                                                                                          >  1:1244430/1‑150 (MQ=255)
              aagagcgtcttAACTAAGATTTCGCTTAAGCGACATCCTGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCgg                                                                                                                                         >  1:194961/8‑150 (MQ=37)
               aGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGga                                                                                                                                        <  2:47800/150‑1 (MQ=255)
                   cttcttAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAgagt                                                                                                                                    <  2:659948/150‑1 (MQ=255)
                   cttcttAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAgagt                                                                                                                                    <  2:666338/150‑1 (MQ=255)
                   cgacttAACAGGCTGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTCGCTTTAAGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAgagt                                                                                                                                    <  2:110392/147‑1 (MQ=255)
                       ttAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAgagtgagt                                                                                                                                >  1:1494305/1‑150 (MQ=255)
                        tAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTc                                                                                                                               >  2:1735142/1‑150 (MQ=255)
                          aCAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTg                                                                                                                             >  1:27130/1‑150 (MQ=255)
                          aCAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTg                                                                                                                             <  1:471036/150‑1 (MQ=255)
                          aCAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTg                                                                                                                             <  2:229109/150‑1 (MQ=255)
                          aCAGGATGTCGCTAAAGCGAAATCTTAGTTAAGCAGCGCTTTGAAACGAGCAGCCTTACCATTACGCTCACGCATGTAGTACAGTTTAGCTTTACTAACCGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTg                                                                                                                             <  1:101657/150‑1 (MQ=255)
                           cAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTgg                                                                                                                            <  2:47154/150‑1 (MQ=255)
                              ggATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACAAGTACGCTCACGCAGGTAGTACAGATTAGCTTTACGATCAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGaa                                                                                                                          >  2:940717/1‑150 (MQ=255)
                               gatcgcGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAg                                                                                                                         <  1:1110427/145‑1 (MQ=255)
                               gATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAg                                                                                                                         <  1:1810829/150‑1 (MQ=255)
                               gATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAg                                                                                                                         <  2:591707/150‑1 (MQ=255)
                               gATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCGCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAg                                                                                                                         <  1:1104649/150‑1 (MQ=255)
                                 tGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGac                                                                                                                       <  1:245644/150‑1 (MQ=255)
                                     gCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCCCGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACAGGAGAGTGAGTCTGGAAGACACGc                                                                                                                   >  1:1631567/1‑150 (MQ=255)
                                        tAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCa                                                                                                                >  1:1017033/1‑150 (MQ=255)
                                           gCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAATTGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACg                                                                                                             >  1:837594/1‑150 (MQ=255)
                                           gCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGATGACACGCTCAACg                                                                                                             >  2:1269961/1‑150 (MQ=255)
                                            cGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACgg                                                                                                            <  1:1523016/150‑2 (MQ=255)
                                             gAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGcc                                                                                                           >  2:139082/1‑150 (MQ=255)
                                                aTCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTc                                                                                                        <  2:838763/150‑1 (MQ=255)
                                                aTCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTc                                                                                                        <  1:68201/150‑1 (MQ=255)
                                                    tAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCg                                                                                                    <  2:1084902/150‑1 (MQ=255)
                                                      gTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGtt                                                                                                  >  1:946090/1‑150 (MQ=255)
                                                      gTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGtt                                                                                                  >  2:1418492/1‑150 (MQ=255)
                                                       ttAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTg                                                                                                 <  1:158618/150‑1 (MQ=255)
                                                       ttAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTg                                                                                                 <  2:117313/150‑1 (MQ=255)
                                                       ttAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTg                                                                                                 >  1:1201988/1‑150 (MQ=255)
                                                          aGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGaa                                                                                              >  2:617236/1‑150 (MQ=255)
                                                            aCGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCATTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAAt                                                                                            >  1:878524/1‑150 (MQ=255)
                                                             cGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAAtt                                                                                           <  1:139082/150‑1 (MQ=255)
                                                               ctctTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAAtttt                                                                                         <  2:370318/150‑1 (MQ=255)
                                                                 ctTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTAc                                                                                       >  2:14480/1‑150 (MQ=255)
                                                                     gATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAAc                                                                                   >  1:1635920/1‑150 (MQ=255)
                                                                     gATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAAc                                                                                   >  1:443206/1‑150 (MQ=255)
                                                                       tACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAg                                                                                 >  1:1506770/1‑150 (MQ=255)
                                                                        aCGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGt                                                                                >  2:453181/1‑150 (MQ=255)
                                                                         cGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTg                                                                               >  1:1122110/1‑150 (MQ=255)
                                                                               gcCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCa                                                                         <  2:799282/150‑1 (MQ=255)
                                                                                     ccAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGc                                                                   >  1:854345/1‑150 (MQ=255)
                                                                                     ccAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGc                                                                   <  1:938039/150‑1 (MQ=255)
                                                                                           cGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGCCcg                                                             >  2:1110638/1‑150 (MQ=255)
                                                                                             cTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACcgcg                                                           >  2:1647779/1‑150 (MQ=255)
                                                                                              tCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCTCGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGAAACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCgg                                                          <  1:802333/150‑1 (MQ=255)
                                                                                              tCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCgg                                                          >  1:388359/1‑150 (MQ=255)
                                                                                                aCGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGtt                                                        <  1:292836/150‑1 (MQ=255)
                                                                                                 cGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTa                                                       >  2:1655500/1‑150 (MQ=255)
                                                                                                  gCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTAc                                                      <  2:194961/150‑1 (MQ=255)
                                                                                                  gCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTAc                                                      <  2:293585/150‑1 (MQ=255)
                                                                                                   gatgtagtaCAGTTTAGCATTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGAGACGCTCAACGCCTTCGCCGTTGTAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACg                                                     <  2:1344540/147‑1 (MQ=255)
                                                                                                   cAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACg                                                     >  1:1817641/1‑150 (MQ=255)
                                                                                                   cAGGTAGTACAGTTAAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGTGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACg                                                     <  2:701816/150‑1 (MQ=255)
                                                                                                    aGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGa                                                    <  2:1173707/150‑1 (MQ=255)
                                                                                                      gtagtaCAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAAt                                                  <  2:1713058/150‑1 (MQ=255)
                                                                                                       tagtaCAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATa                                                 <  2:196955/150‑1 (MQ=255)
                                                                                                         gtaCAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGc                                               <  1:381652/150‑1 (MQ=255)
                                                                                                         gtaCAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGc                                               <  2:1318187/150‑1 (MQ=255)
                                                                                                           aCAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGa                                             >  1:487494/1‑150 (MQ=255)
                                                                                                             aGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATa                                           <  1:1225963/150‑1 (MQ=255)
                                                                                                                   gCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACg                                     >  1:748434/1‑150 (MQ=255)
                                                                                                                   gCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACg                                     >  1:1771210/1‑150 (MQ=255)
                                                                                                                     tttACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGcc                                   >  1:1449928/1‑150 (MQ=255)
                                                                                                                       tACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCt                                 <  1:457328/150‑1 (MQ=255)
                                                                                                                         cGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGCCCGCGGTTACGAATAGCGATAACCACGCCCACg                               >  2:947984/1‑150 (MQ=255)
                                                                                                                             cAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATg                           >  1:664249/1‑150 (MQ=255)
                                                                                                                               gccccacGACGTTTGACAGAAATGCTGTCAACTAACGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACATTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGcc                         <  2:1215748/147‑1 (MQ=255)
                                                                                                                               gCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGCACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAGCCGCGCCCTCGAATGcc                         >  1:1685573/1‑150 (MQ=255)
                                                                                                                               gCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGcc                         <  2:504379/150‑1 (MQ=255)
                                                                                                                               gCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTGGGAAATTTTACGAACAGTGAATGCAGAGTGCATCCCGCGGTAACGAATAGCGATAACCACGCCCTCGAATGcc                         >  2:887107/1‑150 (MQ=255)
                                                                                                                               gCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTACGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCCCGCCCTCGAATGcc                         >  2:1593177/1‑150 (MQ=255)
                                                                                                                                  ccacGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGc                      >  2:501563/1‑150 (MQ=255)
                                                                                                                                   cacGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCATACCGCGGTTACGAAAAGCGATAACCACGCCCTCGAATGCCTGCa                     >  2:347266/1‑150 (MQ=255)
                                                                                                                                     cggtagttGACAGCATTTCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGa                   >  2:1238418/7‑150 (MQ=255)
                                                                                                                                     cgacgTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTAGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCACGAATGCCTGCAGa                   >  2:500455/1‑150 (MQ=255)
                                                                                                                                      ggtagttGACAGCATTTCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGAc                  >  2:684564/6‑150 (MQ=255)
                                                                                                                                       acgTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACg                 <  1:55928/150‑1 (MQ=255)
                                                                                                                                         gTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGtt               <  2:27130/150‑1 (MQ=255)
                                                                                                                                         agttGACAGCATTTCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGtt               >  1:1330041/3‑150 (MQ=255)
                                                                                                                                          tttGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGttt              <  1:1793582/150‑1 (MQ=255)
                                                                                                                                              agaGAAATTCTGTCAACTTCCGGAGACTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTg          <  1:940717/148‑1 (MQ=255)
                                                                                                                                              aCATAAATTCTGGCAACTCCCGGCGTGTGAGTCTGGACTCCACGCTCAACGCCTTCGAGGTTGGAAATTTTACGAACAGTGAATGCAGATTGCAGACCGCGGTTACGAATAGCGATAACCCCGCCCTCGAATGCCTGCAGACGTTTTTTg          <  2:1730099/150‑1 (MQ=255)
                                                                                                                                              aCAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTg          <  1:1115293/150‑1 (MQ=255)
                                                                                                                                              aCAGAAATGCTGTCAACTACCGGAGAGTGAGGCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTg          <  2:1447411/150‑1 (MQ=255)
                                                                                                                                               cAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTgg         >  1:1403633/1‑150 (MQ=255)
                                                                                                                                               cAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTATgg         >  1:80798/1‑150 (MQ=255)
                                                                                                                                                aGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCATCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGa        <  1:154204/150‑1 (MQ=255)
                                                                                                                                                  aaaTGCTGTCAACTACCTGAGAGGGAGTCTGGAAGACACGCTCAACGCCTTCGCAGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAAc      <  1:262455/150‑1 (MQ=255)
                                                                                                                                                   aaTGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAAcc     >  1:1749515/1‑150 (MQ=255)
                                                                                                                                                     tGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGAAGAGTGCAGCCCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCtt   >  1:883254/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTC  >  NC_000913/2744133‑2744431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: