Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,815,730 C→T 23.8% S237N (AGC→AAC)  gshA ← glutamate‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,815,7300CT23.8% 210.2 / 63.4 114S237N (AGC→AAC) gshAglutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (40/46);  new base T (12/15);  total (52/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 116 total aligned reads displayed.

GCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATA  >  NC_000913/2815581‑2815878
                                                                                                                                                     |                                                                                                                                                    
gCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGc                                                                                                                                                      <  1:871146/150‑1 (MQ=255)
gCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGc                                                                                                                                                      >  2:571793/1‑150 (MQ=255)
 cAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCt                                                                                                                                                     >  1:403435/1‑150 (MQ=255)
  aGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTc                                                                                                                                                    <  1:976227/150‑1 (MQ=255)
  aGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTc                                                                                                                                                    <  2:1427708/150‑1 (MQ=255)
  aGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTc                                                                                                                                                    >  1:711793/1‑150 (MQ=255)
        ttACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGa                                                                                                                                              <  1:1028680/150‑1 (MQ=255)
        ttACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTAGTAAAGATAGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGa                                                                                                                                              >  2:1568899/1‑150 (MQ=255)
         tACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGaa                                                                                                                                             <  1:657673/150‑1 (MQ=255)
          aCCGTCTTTCTGAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAag                                                                                                                                            <  1:170947/150‑1 (MQ=255)
          aCCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGTGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAag                                                                                                                                            >  2:1340501/1‑150 (MQ=255)
            cGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAagag                                                                                                                                          <  2:23374/150‑1 (MQ=255)
             gTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAagaga                                                                                                                                         >  1:805428/1‑150 (MQ=255)
                  tctcAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCg                                                                                                                                    <  2:1529467/150‑1 (MQ=255)
                   ctcAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGc                                                                                                                                   >  2:619688/1‑150 (MQ=255)
                   ctcAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGc                                                                                                                                   >  1:58820/1‑150 (MQ=255)
                   ctcAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGc                                                                                                                                   <  1:752210/150‑1 (MQ=255)
                   ctcAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGc                                                                                                                                   >  2:1282027/1‑150 (MQ=255)
                     cAATACCACTCATCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCAt                                                                                                                                 <  2:513771/150‑1 (MQ=255)
                       aTACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATAc                                                                                                                               >  1:813364/1‑150 (MQ=255)
                        tACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGAAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACg                                                                                                                              >  2:490482/1‑150 (MQ=255)
                           cAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACAAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCa                                                                                                                           >  1:150457/1‑150 (MQ=255)
                              tCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGt                                                                                                                        <  1:820735/150‑1 (MQ=255)
                                    cgTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTATTACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAataca                                                                                                                  <  1:480961/150‑1 (MQ=255)
                                    cgTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGGAGGTAataca                                                                                                                  <  1:1078775/150‑1 (MQ=255)
                                    cgTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAataca                                                                                                                  >  1:1167998/1‑150 (MQ=255)
                                        ctctTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATAcc                                                                                                              >  1:272789/1‑150 (MQ=255)
                                        ctctTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGAACGTTGAAGGTAATACCAAGATTGAATTGCGATTTATTGGTATAGCAGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAAAACATAcc                                                                                                              >  1:254827/1‑150 (MQ=255)
                                          ctTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGc                                                                                                            <  1:685432/150‑1 (MQ=255)
                                          ctTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGc                                                                                                            >  1:1139630/1‑150 (MQ=255)
                                                   gCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTGGAAGGTAATTCCTAGATTGCTTTGCGATTTATTGTTATAGCGGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGttt                                                                                                   <  2:748098/150‑1 (MQ=255)
                                                      ttttGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTtct                                                                                                <  2:1490537/150‑1 (MQ=255)
                                                      ttttGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTtct                                                                                                <  1:3529/150‑1 (MQ=255)
                                                      ttttGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTtct                                                                                                <  1:575989/150‑1 (MQ=255)
                                                      gtttgtttgCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGTTAATACCAAGAATTCTTAGAGATTTATTGGTATAGCCGAGATCGCTCAAAAGAATAGAGGTCGCATACTGCAGGTAATACATACCGCACTCTTTTTtct                                                                                                <  2:1203474/149‑1 (MQ=255)
                                                        ttGATTGCCTGTTTAAGGCCCGCTTCGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCa                                                                                              <  1:640777/150‑1 (MQ=255)
                                                         tGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCaa                                                                                             <  1:455374/150‑1 (MQ=255)
                                                           aTTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCTTTGCAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGACAGGTCGCATACTGCAGGTAATACATACCGCACTCGGTTTTCTCAAAc                                                                                           <  1:159842/150‑1 (MQ=255)
                                                           aTTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTTCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAAc                                                                                           <  2:1396121/150‑1 (MQ=255)
                                                           aTTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAAc                                                                                           >  1:1766521/1‑150 (MQ=255)
                                                            ttGCCTGTTTAACGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGAACGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACg                                                                                          <  2:665475/150‑1 (MQ=255)
                                                              gCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGc                                                                                        <  1:1282027/150‑1 (MQ=255)
                                                               ccTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGcc                                                                                       >  2:91458/1‑149 (MQ=255)
                                                               ccTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCa                                                                                       >  1:802566/1‑150 (MQ=255)
                                                                  gTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCg                                                                                    >  1:1540490/1‑150 (MQ=255)
                                                                  gTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCg                                                                                    <  2:1411505/150‑1 (MQ=255)
                                                                         gCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTgg                                                                             <  2:58820/150‑1 (MQ=255)
                                                                           ccGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGtt                                                                           >  1:910306/1‑150 (MQ=255)
                                                                              cTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATACCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTc                                                                        <  2:1533758/150‑1 (MQ=255)
                                                                                  gTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTg                                                                    <  1:449689/150‑1 (MQ=255)
                                                                                   tACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGc                                                                   >  2:706933/1‑150 (MQ=255)
                                                                                   tACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGc                                                                   <  2:627652/150‑1 (MQ=255)
                                                                                   tACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCACAAACGAAGAGAGGTCGCATACGGAAGGTAATACATACCGCACTCGGATTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGc                                                                   >  2:1567974/1‑150 (MQ=255)
                                                                                    aCTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCa                                                                  >  1:1571515/1‑150 (MQ=255)
                                                                                    aCTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCa                                                                  <  1:735278/150‑1 (MQ=255)
                                                                                     ctagTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAg                                                                 <  2:918236/147‑1 (MQ=255)
                                                                                     cTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAg                                                                 >  1:1737107/1‑150 (MQ=255)
                                                                                     cTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAg                                                                 >  2:627359/1‑150 (MQ=255)
                                                                                     cTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGAGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCAATCGGTTTTCTCAAACGGCAGCGACGTTCGTTTTCCTTGCAg                                                                 >  1:181941/1‑150 (MQ=255)
                                                                                       cGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACTGCAGCGACGTTGGTTTTCCTTGCAGGa                                                               >  1:1477879/1‑150 (MQ=255)
                                                                                       cGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGa                                                               >  1:483526/1‑150 (MQ=255)
                                                                                          aaaGATCGTTGTAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAaag                                                            <  2:888221/150‑1 (MQ=255)
                                                                                          aaaGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAaag                                                            <  1:1294463/150‑1 (MQ=255)
                                                                                           aaGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAaaga                                                           <  1:619688/150‑1 (MQ=255)
                                                                                            aGATCGTTGAATGTAATACCAAGATTGCTTTGAGATTTATTTGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCATACGGCAGCGACGTTGGTTTTCCTTGCAGGAaagaa                                                          <  1:114794/150‑1 (MQ=255)
                                                                                                cGTTGAAGGTAATACCAAGATTGCTTTTCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAAc                                                      <  2:1757055/150‑1 (MQ=255)
                                                                                                cGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAAc                                                      <  2:1473396/150‑1 (MQ=255)
                                                                                                cGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGACGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAAc                                                      >  1:1347342/1‑150 (MQ=255)
                                                                                                     aaGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATc                                                 >  2:1316437/1‑150 (MQ=255)
                                                                                                      aGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCg                                                <  2:1338971/150‑1 (MQ=255)
                                                                                                       ggTAATAGAAAGATTGCTGTTCGATTTATTGTTATAGCCGAGATCGCTCAAACGATGTGAGGTCGCATACTGCAGGTAATACATATCGCACTCGTTTTTATCAATCTGCAGAGACGTTGGTTTTCCTTGCAGGAAAGATTTACAAATCGc                                               <  1:286647/150‑1 (MQ=255)
                                                                                                       ggTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGc                                               <  1:672031/150‑1 (MQ=255)
                                                                                                         tAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCg                                             <  2:473134/150‑1 (MQ=255)
                                                                                                           aTACCAAGATTGCTTTGCTATTTATTGGTATATCCGAGATCGATCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGga                                           <  1:1568899/150‑1 (MQ=255)
                                                                                                            tACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGgag                                          >  1:5842/1‑150 (MQ=255)
                                                                                                            tACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGgag                                          <  2:813364/150‑1 (MQ=255)
                                                                                                            tACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGgag                                          >  2:445705/1‑150 (MQ=255)
                                                                                                             aCCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGgaga                                         <  1:749114/150‑1 (MQ=255)
                                                                                                               cAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATg                                       <  2:180624/150‑1 (MQ=255)
                                                                                                               cAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATg                                       <  2:1019701/150‑1 (MQ=255)
                                                                                                                 aGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGcc                                     >  2:1692088/1‑149 (MQ=255)
                                                                                                                   aTTGCTTTGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCAcc                                   <  2:1240520/150‑1 (MQ=255)
                                                                                                                        tttGCGATTTATTGGTATAGCCGAGATCGTGCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACa                              <  2:517584/150‑1 (MQ=255)
                                                                                                                          tGCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGa                            >  1:814969/1‑150 (MQ=255)
                                                                                                                           gCGATTTATTGGTATAGCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACAGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGAt                           >  1:203761/1‑150 (MQ=255)
                                                                                                                                  aTTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAAt                    <  1:1340501/150‑1 (MQ=255)
                                                                                                                                   ttGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCGTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATg                   >  1:1305732/1‑150 (MQ=255)
                                                                                                                                   ttGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATg                   <  2:1212092/150‑1 (MQ=255)
                                                                                                                                    tGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATgc                  >  1:1252329/1‑149 (MQ=255)
                                                                                                                                       ttttgCCGAGCTGGCTCAAACGAAGAGATGTCTCATACTGCAGGTAATACATACGGCACTCGGTTTTCTCAAACGGCTGTGACTTTTGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGAccc               <  2:254827/146‑1 (MQ=255)
                                                                                                                                          aGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAAc            >  1:1009589/1‑150 (MQ=255)
                                                                                                                                           gCCGAGATCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAAcc           >  1:139208/1‑150 (MQ=255)
                                                                                                                                           gCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAAcc           >  1:1596214/1‑150 (MQ=255)
                                                                                                                                           gCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAAcc           <  2:552330/150‑1 (MQ=255)
                                                                                                                                             cGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGa         >  2:1822166/1‑150 (MQ=255)
                                                                                                                                                gaTCGTTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAAc      <  1:114408/150‑1 (MQ=255)
                                                                                                                                                gaTCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAAc      <  1:490482/150‑1 (MQ=255)
                                                                                                                                                 aTCGCTCATACGAAGAGAGGTCGCATACGGAAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCTGCGACGTTGGATTTCCTTGCAGGATAGAAGAACAAATCGCCGGAGATGCAAAAAACAGATAAGAAAAGTCCCAACCGAAACg     >  1:1675437/1‑150 (MQ=255)
                                                                                                                                                  tCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGa    >  1:206891/1‑150 (MQ=255)
                                                                                                                                                    gCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATa  >  2:171072/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATA  >  NC_000913/2815581‑2815878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: