Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,828,533 A→T 5.1% intergenic (+17/‑88) srlD → / → srlM sorbitol‑6‑phosphate dehydrogenase/sorbitol=responsive srl operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,828,5330AT 5.1% 377.2 / 6.5 118intergenic (+17/‑88)srlD/srlMsorbitol‑6‑phosphate dehydrogenase/sorbitol=responsive srl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (66/46);  new base T (4/2);  total (70/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 118 total aligned reads displayed.

GACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCG  >  NC_000913/2828385‑2828681
                                                                                                                                                    |                                                                                                                                                     
gACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCAt                                                                                                                                                      >  1:72743/1‑150 (MQ=255)
  cAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTa                                                                                                                                                    <  2:48223/150‑1 (MQ=255)
  cAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTa                                                                                                                                                    <  2:578190/150‑1 (MQ=255)
    aaGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAg                                                                                                                                                 <  1:417817/150‑1 (MQ=255)
        accgagcAAACGCGGCTGAGATTATCAAGATGTGCTGAATATGCTTCTGGTCTACGCCAGTCCTAAGGCGTCGTACTGCTCGGGACAGTCGAGCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAtc                                                                                                                                             <  2:1590081/144‑1 (MQ=255)
        aCCGCTCAACCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGTTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAtc                                                                                                                                             <  2:896781/150‑1 (MQ=255)
          cGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAtctc                                                                                                                                           >  2:1056517/1‑150 (MQ=255)
            cTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                         <  1:432122/150‑1 (MQ=255)
             tCAAACGCTGCTGCGATTTTCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCTAGCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGt                                                                                                                                        <  1:963303/150‑1 (MQ=255)
              cAAACGCGGCTGCGATTATCTAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCTTAATGGATCTCCgct                                                                                                                                       <  2:1387196/150‑3 (MQ=255)
              cAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTg                                                                                                                                       >  2:69305/1‑150 (MQ=255)
                aaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTgag                                                                                                                                     <  1:1129706/150‑1 (MQ=255)
                aaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTgag                                                                                                                                     <  2:506754/150‑1 (MQ=255)
                         gCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGaa                                                                                                                            >  2:1195807/1‑150 (MQ=255)
                             ttATCAAGATGTGCTGAATTTGCGGCTGTTCTACGCCAGGCCTAAGGCGGCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGcc                                                                                                                        <  2:1227875/150‑1 (MQ=255)
                             ttATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTTCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGcc                                                                                                                        <  2:1115687/150‑1 (MQ=255)
                               aTCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTgag                                                                                                                                     <  2:390120/135‑1 (MQ=255)
                                tCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGa                                                                                                                     >  1:1760561/1‑150 (MQ=255)
                                  aaGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATg                                                                                                                   >  1:481425/1‑150 (MQ=255)
                                   aGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATgc                                                                                                                  <  1:228805/150‑1 (MQ=255)
                                    gATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATgcg                                                                                                                 >  1:978356/1‑150 (MQ=255)
                                    gATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGCCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATgcg                                                                                                                 >  2:664543/1‑150 (MQ=255)
                                     aTGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATgcgc                                                                                                                <  1:601229/150‑1 (MQ=255)
                                       gtgCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTa                                                                                                              >  2:1367874/1‑150 (MQ=255)
                                        tgCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTAc                                                                                                             >  2:854820/1‑150 (MQ=255)
                                         gCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACg                                                                                                            >  1:1695333/1‑150 (MQ=255)
                                         gCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACg                                                                                                            >  1:1133773/1‑150 (MQ=255)
                                          cagAAAATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGTCGGTCAGGTGATGTTCTGATCAACAGCGGACATCCATTAAGCATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGc                                                                                                           <  1:298366/148‑1 (MQ=255)
                                          cTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGc                                                                                                           >  1:1603987/1‑150 (MQ=255)
                                            gAATATGCTGCTGTTCAACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGAGCAATGTCACCGGCGGTCAGGTGAAGTACTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCAGATGCGCTACgata                                                                                                         >  1:1002757/1‑147 (MQ=255)
                                                 tgctgcTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg                                                                                                    <  1:1400017/150‑1 (MQ=255)
                                                   ctgctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGAGCGTAGCGCATCAGGc                                                                                                  <  1:1012656/150‑1 (MQ=255)
                                                    tgctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGAGCTACGCTCATCAGGCa                                                                                                 >  2:869005/1‑150 (MQ=255)
                                                     gctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCAt                                                                                                >  2:375776/1‑150 (MQ=255)
                                                        gTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                      <  1:1570200/109‑1 (MQ=255)
                                                        gTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                      >  2:1570200/1‑109 (MQ=255)
                                                            tACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGAc                                                                                         >  1:90922/1‑150 (MQ=255)
                                                              cGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTTCGCTCATCAGGCATACAGGACtt                                                                                       >  2:357089/1‑150 (MQ=255)
                                                                ccAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTcc                                                                                     >  2:899789/1‑150 (MQ=255)
                                                                     ccTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCAc                                                                                >  1:324005/1‑150 (MQ=255)
                                                                      cTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACt                                                                               <  1:1703234/150‑1 (MQ=255)
                                                                            cgtcgtACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTa                                                                         <  1:1641272/150‑1 (MQ=255)
                                                                            cgtcgtACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTa                                                                         <  2:1760561/150‑1 (MQ=255)
                                                                               cgtACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAgg                                                                      >  2:1438116/1‑150 (MQ=255)
                                                                               cgtACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAgg                                                                      <  2:1424554/150‑1 (MQ=255)
                                                                               cgtACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAgg                                                                      <  2:1296995/150‑1 (MQ=255)
                                                                                  aCTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaaa                                                                   >  2:290499/1‑150 (MQ=255)
                                                                                  aCTGCACCGGACAGTCGATCAATGTCACCGGCGGGCAGGTGATGTGCTGATCAACAGCGGAGATCCATTACGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaaa                                                                   <  1:38578/150‑1 (MQ=255)
                                                                                   cTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaaaa                                                                  >  1:1064309/1‑150 (MQ=255)
                                                                                     gCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGt                                                                <  1:762001/150‑1 (MQ=255)
                                                                                          ggACAGTCGATCAATGTCACCGGCGGTCAGGTGTTGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATgg                                                           >  2:441561/1‑150 (MQ=255)
                                                                                          ggACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATgg                                                           <  1:666229/150‑1 (MQ=255)
                                                                                               gTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCTGAGATCCATTAAGGATCTCCGTGAGACTATATAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATcc                                                      <  1:871309/150‑1 (MQ=255)
                                                                                               gTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATcc                                                      >  1:811649/1‑150 (MQ=255)
                                                                                               gTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATcc                                                      >  2:1066562/1‑150 (MQ=255)
                                                                                                  gATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCa                                                   >  1:58071/1‑150 (MQ=255)
                                                                                                    tCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATTGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACt                                                 <  2:1027071/150‑1 (MQ=255)
                                                                                                    tCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACt                                                 <  2:1010764/150‑1 (MQ=255)
                                                                                                         gTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGGCACTACATTAAGGAAAAGTTATGGTATCCGCACtcatca                                            >  2:927337/1‑150 (MQ=255)
                                                                                                         gTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtcatca                                            >  2:1149293/1‑150 (MQ=255)
                                                                                                         gTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtcatca                                            <  2:389016/150‑1 (MQ=255)
                                                                                                            aCCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCg                                         >  1:1459442/1‑150 (MQ=255)
                                                                                                            aCCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCg                                         >  2:1555295/1‑150 (MQ=255)
                                                                                                             ccGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGt                                        >  1:1008941/1‑150 (MQ=255)
                                                                                                             ccGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGt                                        >  1:458188/1‑150 (MQ=255)
                                                                                                                 cggTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGcc                                    <  1:622834/150‑1 (MQ=255)
                                                                                                                  ggTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCg                                   <  2:222369/150‑1 (MQ=255)
                                                                                                                   gTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGt                                  <  1:425858/150‑1 (MQ=255)
                                                                                                                   gTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGt                                  <  2:902326/150‑1 (MQ=255)
                                                                                                                     cAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATTCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTa                                <  2:1127676/150‑1 (MQ=255)
                                                                                                                     cAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTa                                >  2:846814/1‑150 (MQ=255)
                                                                                                                       ggTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATc                              <  2:1599613/150‑1 (MQ=255)
                                                                                                                             gTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTgg                        >  1:1385948/1‑150 (MQ=255)
                                                                                                                              ttCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGgt                       >  2:294022/1‑150 (MQ=255)
                                                                                                                                 tGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGtgtg                    <  1:1195807/150‑1 (MQ=255)
                                                                                                                                 tGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGtgtg                    >  2:377317/1‑150 (MQ=255)
                                                                                                                                 tGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGtgtg                    >  1:628697/1‑150 (MQ=255)
                                                                                                                                  gATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTgc                   >  2:1095025/1‑150 (MQ=255)
                                                                                                                                  gATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTgc                   >  2:1612710/1‑150 (MQ=255)
                                                                                                                                  gATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTgc                   >  2:851135/1‑150 (MQ=255)
                                                                                                                                     cAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCa                >  1:434013/1‑150 (MQ=255)
                                                                                                                                     cAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCa                >  1:137451/1‑150 (MQ=255)
                                                                                                                                      tctcacGGAGATCATTAATGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCAGCAGGCATACAGGACTTCCGCAACTACATTAAGGAAAAGTTATGGTAACCGCACTCATCACCGTCGCCCTTAACGCCTGGAGTGCGCaa               >  2:175408/6‑150 (MQ=255)
                                                                                                                                       aCAGCGGAGATCCATTAATGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAAc              <  2:1182649/150‑1 (MQ=255)
                                                                                                                                        cAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACt             >  2:1515048/1‑150 (MQ=255)
                                                                                                                                         cacGGAGATCCTTAATGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTg            >  2:591390/3‑150 (MQ=255)
                                                                                                                                         aGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTg            >  1:538670/1‑150 (MQ=255)
                                                                                                                                          gCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTgg           <  1:1056517/150‑1 (MQ=255)
                                                                                                                                          gCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACAGTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCTCCTGGTGTGCGCAACTgg           >  1:450359/1‑150 (MQ=255)
                                                                                                                                          acGGAGATCCTTAATGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTgg           >  1:557085/2‑150 (MQ=255)
                                                                                                                                            ggAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGcc         <  1:290499/150‑1 (MQ=255)
                                                                                                                                               gaTCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTa      >  2:702261/1‑150 (MQ=255)
                                                                                                                                               caTCCAATAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAACGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTa      <  1:282312/149‑1 (MQ=255)
                                                                                                                                                 tCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAgg    <  1:1613649/150‑1 (MQ=255)
                                                                                                                                                  ccATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTCCGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAggc   >  1:1794648/1‑150 (MQ=255)
                                                                                                                                                   cATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAggcg  >  2:713912/1‑150 (MQ=255)
                                                                                                                                                   cATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAggcg  >  1:21275/1‑150 (MQ=255)
                                                                                                                                                   cATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAggcg  >  2:1198002/1‑150 (MQ=255)
                                                                                                                                                   cATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAggcg  >  2:1460598/1‑150 (MQ=255)
                                                                                                                                                   cATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGATATGTTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAggcg  >  1:1612949/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCG  >  NC_000913/2828385‑2828681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: