Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,838,231 A→T 5.7% intergenic (‑126/+23) hydN ← / ← ascG formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,838,2310AT 5.7% 382.1 / 9.0 136intergenic (‑126/+23)hydN/ascGformate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base A (69/57);  new base T (5/3);  total (75/61)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 137 total aligned reads displayed.

GTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGT  >  NC_000913/2838082‑2838379
                                                                                                                                                     |                                                                                                                                                    
gTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGa                                                                                                                                                      >  1:1514473/1‑150 (MQ=255)
 tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGAc                                                                                                                                                     <  1:756263/150‑1 (MQ=255)
     cAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATg                                                                                                                                                 >  2:1688081/1‑150 (MQ=255)
     cAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATg                                                                                                                                                 >  2:44539/1‑150 (MQ=255)
     cAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATg                                                                                                                                                 <  2:1483283/150‑1 (MQ=255)
       atgatgAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGAc                                                                                                                                               <  1:1002517/150‑1 (MQ=255)
         gatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAt                                                                                                                                             >  1:1516789/1‑150 (MQ=255)
           tgaCACGGTTCTTCAAATTCTGCAGCAATGACAGTTAATGCCCCGATACATACACAAATCATGCCAGTTTTTATTTTAGTGTTATTTAAGGAAATTAATTTCTGTAATGCAGTAAAAACTATGTCATCGACACTAGTGACGATGACAagt                                                                                                                                           >  2:897090/1‑150 (MQ=255)
           tgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGa                                                                                                                                                <  2:242150/145‑1 (MQ=255)
           tgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACAATGTCATCACAAGAAGTAACGATGActttt                                                                                                                                           >  2:1326867/1‑146 (MQ=255)
             aaaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtga                                                                                                                                         <  1:330932/150‑1 (MQ=255)
             aaaCGGATCATCAAAATCTCCAGCAATGACAGTTAATGCTCAGATACATTCACAAATCATGCAAGTTTTTAATTTACTTTTATTAAATGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtga                                                                                                                                         <  2:1305786/150‑1 (MQ=255)
              aaCGGATCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgat                                                                                                                                        <  1:394456/150‑1 (MQ=255)
                cGGTTCATCAAATTCTACAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtcgttttt                                                                                                                                      <  1:1088718/150‑8 (MQ=255)
                      atcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACTATGTCAGCGACACTAGTGACGATTACATGTGATGAAAATGt                                                                                                                                >  1:974726/1‑150 (MQ=255)
                      atcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGt                                                                                                                                >  1:202330/1‑150 (MQ=255)
                      atcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGt                                                                                                                                >  1:1697611/1‑150 (MQ=255)
                       tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                               <  1:104128/150‑1 (MQ=255)
                       tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                               >  2:521710/1‑150 (MQ=255)
                       tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                               <  2:757381/150‑1 (MQ=255)
                       acaAATTCGCCAGCAAAGACACTTAATGCGCCGATACATTCACAAATCATGCCACTTTTCAATTTACTGTTATTTAACGAAATTAATTTCTGTAATGCAGGAAAAACGATGGCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                               <  2:122689/149‑1 (MQ=255)
                        caAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACACATCATGCCAGTAGTTAATATACTGTTTTTTAAGGAAATAAATTTCTGTCATGCAGGAAAACAGATGTCATCGACACTAGTGACGAAGACATGTGATGACAATGttt                                                                                                                              >  1:1101985/1‑150 (MQ=255)
                           aTTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATc                                                                                                                           >  1:1662995/1‑150 (MQ=255)
                             tctcCAGCAATGACAGGTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcgc                                                                                                                         <  2:152623/150‑1 (MQ=255)
                              ctcCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcgcg                                                                                                                        >  2:1240764/1‑150 (MQ=255)
                               tcCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGa                                                                                                                       >  1:1622481/1‑150 (MQ=255)
                                ccAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTATGCAAATTAATTTCTGTAAAGCAGGAAAAACGATGACATCGACACTAGTGACGATGACATGTCATGACAATGTTTATCTCGaa                                                                                                                      >  1:856799/1‑150 (MQ=255)
                                 cAGCAATGACAGTTAATGCGCCGATACATTCACAACTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAg                                                                                                                     >  1:1716576/1‑150 (MQ=255)
                                 cAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCTCCAATAGTTACGATGACATGTGATTACAATGTTTATCGAGAAg                                                                                                                     >  1:1709698/1‑150 (MQ=255)
                                   gCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGa                                                                                                                   >  2:1094981/1‑150 (MQ=255)
                                    cAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAg                                                                                                                  <  2:739763/150‑1 (MQ=255)
                                     aaTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGc                                                                                                                 <  2:1496315/150‑1 (MQ=255)
                                     aaTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGc                                                                                                                 <  1:649382/150‑1 (MQ=255)
                                            gTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagt                                                                                                          <  1:1688081/150‑1 (MQ=255)
                                            gTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagt                                                                                                          <  2:1047620/150‑1 (MQ=255)
                                              tAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagtga                                                                                                        >  2:678755/1‑150 (MQ=255)
                                                   cgcCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTcgc                                                                                                   >  1:1415496/1‑150 (MQ=255)
                                                   cgcCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGAAAATGTTTATCTCGAAGGAGCAATGAGTGAGTcgc                                                                                                   >  1:1422786/1‑150 (MQ=255)
                                                      cGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAAATAATTTCTGTAATGCAGGAACAACGCTGGCATCGACACTAGTGCCGAGGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGGGAGTCgcggc                                                                                                <  1:106517/150‑1 (MQ=255)
                                                       gATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCgcggcg                                                                                               <  1:1238596/150‑1 (MQ=255)
                                                       gATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCgcggcg                                                                                               <  1:1276213/150‑1 (MQ=255)
                                                         tACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGa                                                                                             <  2:685817/150‑1 (MQ=255)
                                                           cATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATc                                                                                           <  2:552253/150‑1 (MQ=255)
                                                               cacaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtt                                                                                       >  2:1485201/1‑150 (MQ=255)
                                                               cacaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtt                                                                                       >  2:234793/1‑150 (MQ=255)
                                                                 caAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtttt                                                                                     >  2:1266456/1‑150 (MQ=255)
                                                                 caAATCATGCCAGTTTTAAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtttt                                                                                     >  1:898903/1‑150 (MQ=255)
                                                                      cATGCCAGTTTTTAATTTACTGTTAATTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTACgat                                                                                >  1:1669544/1‑148 (MQ=255)
                                                                         gCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTACTGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGaa                                                                             <  1:88427/150‑1 (MQ=255)
                                                                           cactttttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAgg                                                                           <  2:334818/147‑1 (MQ=255)
                                                                             gTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGtt                                                                         >  2:31204/1‑150 (MQ=255)
                                                                                tttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTc                                                                      >  2:1190162/1‑150 (MQ=255)
                                                                                    aTTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcg                                                                  >  2:966938/1‑150 (MQ=255)
                                                                                     tttACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcgg                                                                 >  2:1468098/1‑150 (MQ=255)
                                                                                       tACTGTTATTTAAGGAAATTAATTTCTGTAAAGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTg                                                               <  2:1307470/150‑1 (MQ=255)
                                                                                         cTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTgag                                                             >  2:168296/1‑150 (MQ=255)
                                                                                          tGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTgaga                                                            <  2:609760/150‑1 (MQ=255)
                                                                                           gTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGCCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGaa                                                           >  1:1441624/1‑150 (MQ=255)
                                                                                            ttATTTAAGGAAAATAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGCTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGaaa                                                          <  1:335916/150‑1 (MQ=255)
                                                                                              aTTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATc                                                        >  1:179131/1‑150 (MQ=255)
                                                                                                 tAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGTGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATcccc                                                     <  2:78918/150‑1 (MQ=255)
                                                                                                 tAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATcccc                                                     >  1:1291569/1‑150 (MQ=255)
                                                                                                  aaGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGCAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCg                                                    <  1:415114/150‑1 (MQ=255)
                                                                                                   aGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGc                                                   >  2:782636/1‑150 (MQ=255)
                                                                                                    ggAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGcc                                                  >  1:844338/1‑150 (MQ=255)
                                                                                                    ggAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCAGATCAGTTTTCAGCTGAAGGGTTTCGGCGGTGAGATATCCCCGcc                                                  >  2:283052/1‑150 (MQ=255)
                                                                                                      aaaTTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCAt                                                >  2:523905/1‑150 (MQ=255)
                                                                                                      aaaTTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCAt                                                <  1:187410/150‑1 (MQ=255)
                                                                                                        attaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCg                                              <  1:44539/150‑1 (MQ=255)
                                                                                                           aattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGc                                           >  2:480498/1‑150 (MQ=255)
                                                                                                            attTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCa                                          <  1:521710/150‑1 (MQ=255)
                                                                                                             attCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGAAAATGTTTATCGCGAAGGAGAAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCAt                                         >  1:1035772/2‑150 (MQ=255)
                                                                                                                  ttAATTCAGGAAAAACGATGTCATCGACACTAGTTACGATGAAATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaaaaa                                    <  1:1610204/149‑1 (MQ=255)
                                                                                                                    aaTGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATc                                  >  2:1741524/1‑150 (MQ=255)
                                                                                                                      tGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAg                                <  2:502868/150‑1 (MQ=255)
                                                                                                                        cAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGcc                              >  2:1341793/1‑150 (MQ=255)
                                                                                                                          ggAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGt                            >  2:1354313/1‑150 (MQ=255)
                                                                                                                          ggAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGt                            >  1:509871/1‑150 (MQ=255)
                                                                                                                            tcatcacaTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTcc                          >  2:1272816/8‑150 (MQ=255)
                                                                                                                            aaaaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCTTcc                          <  1:266583/150‑1 (MQ=255)
                                                                                                                                cacaTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaata                      >  1:328979/4‑150 (MQ=255)
                                                                                                                                  caTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAATATCAGCCGTCCaagaat                    >  2:1137742/2‑150 (MQ=255)
                                                                                                                                      tCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTcc                >  1:770519/1‑150 (MQ=255)
                                                                                                                                       cATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt               >  2:1217660/1‑150 (MQ=255)
                                                                                                                                       cATCGACACTAGTGACGATGACATGTGATGACAATGTTTNNCGCNAAGGAGCAATGAGTGGGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt               >  2:529043/1‑150 (MQ=255)
                                                                                                                                       cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt               <  2:985019/150‑1 (MQ=255)
                                                                                                                                       cATCGACACTAGTGACGATGACATGTGATGACAATGTTAATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCAGTCCAATAATTTCCt               <  2:1640632/150‑1 (MQ=255)
                                                                                                                                        aTCGACACTAGTGACGATGAGATGTGAGGTCAATGTTTATCGCGAAGGAGCAATGAGTGAGGCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTg              <  2:893509/150‑1 (MQ=255)
                                                                                                                                        aTCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTg              <  1:1207842/150‑1 (MQ=255)
                                                                                                                                         tCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGa             >  2:1183852/1‑150 (MQ=255)
                                                                                                                                          cGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGaa            <  2:812332/150‑1 (MQ=255)
                                                                                                                                          cGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGaa            <  2:853193/150‑1 (MQ=255)
                                                                                                                                           gACACTAGTGACGATGACATGTGATGACACTGTTTATCGCGAAGGAGCAATGAGTGAGGCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAat           <  2:1746512/150‑1 (MQ=255)
                                                                                                                                            acacTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGTATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAatc          <  2:478785/150‑1 (MQ=255)
                                                                                                                                             cacTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAatca         <  1:1240764/150‑1 (MQ=255)
                                                                                                                                                  gTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCa    <  1:261531/150‑1 (MQ=255)
                                                                                                                                                  gTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCa    >  1:1568507/1‑150 (MQ=255)
                                                                                                                                                   tGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAg   <  2:1622481/150‑1 (MQ=255)
                                                                                                                                                    gACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGt  >  2:360132/1‑150 (MQ=255)
                                                                                                                                                    gACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGt  >  2:11553/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGT  >  NC_000913/2838082‑2838379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: