Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,894,852 T→G 5.2% intergenic (+81/+67) ygcP → / ← ygcQ putative antiterminator regulatory protein/putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,894,8520TG 5.2% 383.6 / 8.2 128intergenic (+81/+67)ygcP/ygcQputative antiterminator regulatory protein/putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (58/63);  new base G (2/5);  total (60/68)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 133 total aligned reads displayed.

AACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGCCTGT  >  NC_000913/2894703‑2895000
                                                                                                                                                     |                                                                                                                                                    
aaCGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCt                                                                                                                                                      >  2:953519/1‑150 (MQ=255)
aaCGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCt                                                                                                                                                      >  2:402758/1‑150 (MQ=255)
aaCGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCt                                                                                                                                                      <  2:1596024/150‑1 (MQ=255)
aaCGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCt                                                                                                                                                      >  2:1012742/1‑150 (MQ=255)
 aCGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCtt                                                                                                                                                     >  1:1019759/1‑150 (MQ=255)
  cgcgGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGATAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTg                                                                                                                                                    >  1:816852/1‑150 (MQ=255)
  cgcgGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAGAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTg                                                                                                                                                    >  1:823823/1‑150 (MQ=255)
    cgGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAc                                                                                                                                                  >  2:511822/1‑150 (MQ=255)
    cgGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAc                                                                                                                                                  >  2:1517818/1‑150 (MQ=255)
         gtCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCATTTGAATTTGGTGTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGtg                                                                                                                                             >  1:1057092/1‑149 (MQ=255)
         gtCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc                                                                                                                                             >  2:1547421/1‑150 (MQ=255)
            gTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCCCATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTa                                                                                                                                          >  2:1386626/1‑150 (MQ=255)
            gTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTa                                                                                                                                          >  1:679515/1‑150 (MQ=255)
              ggCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTcgcatc                                                                                                                                        <  2:1009335/150‑6 (MQ=255)
              ggCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAGTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTagc                                                                                                                                        >  2:38694/1‑148 (MQ=255)
                cgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                      >  1:227869/1‑150 (MQ=255)
                cgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTGTGACGGGATAATCGTAGCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                      >  1:1212307/1‑150 (MQ=255)
                 gcTTGCCCCCACGAATACCGGGGTCTGGACGTTCGCGAAAAAATTACTTTGACGGGATAATCGTAAACAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAgg                                                                                                                                     <  2:176252/150‑1 (MQ=255)
                  cTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                    >  2:1574823/1‑150 (MQ=255)
                  cTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                    <  1:816600/150‑1 (MQ=255)
                  cTTTCCACCACGAATACCGGGGTCTGGACGGTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                    <  1:1007228/150‑1 (MQ=255)
                   tttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc                                                                                                                                   <  1:790238/150‑1 (MQ=255)
                    ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcgc                                                                                                                                  <  1:1374726/150‑3 (MQ=255)
                    ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcgc                                                                                                                                  <  1:1326113/150‑3 (MQ=255)
                     tCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTa                                                                                                                                 >  1:439810/1‑150 (MQ=255)
                     tCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGACCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcgct                                                                                                                                 <  1:984222/150‑4 (MQ=38)
                       caccacGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAcc                                                                                                                               >  2:198046/1‑149 (MQ=255)
                        accacGAATATCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaa                                                                                                                              >  1:17757/1‑150 (MQ=255)
                            cGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTcc                                                                                                                          >  2:1382539/1‑150 (MQ=255)
                                tACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAg                                                                                                                      >  2:1267080/1‑150 (MQ=255)
                                 aCCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg                                                                                                                     <  1:1804782/150‑1 (MQ=255)
                                 aCCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg                                                                                                                     <  1:560667/150‑1 (MQ=255)
                                    ggggTCTGGACGTTAGCGAAATAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGGCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCa                                                                                                                  <  1:727115/150‑1 (MQ=255)
                                         cTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAcc                                                                                                             >  2:593081/1‑150 (MQ=255)
                                         cTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCCACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAcc                                                                                                             >  2:614567/1‑150 (MQ=255)
                                          tGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg                                                                                                            <  2:1028156/150‑1 (MQ=255)
                                             aCGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg                                                                                                         <  2:871366/150‑1 (MQ=255)
                                                    cGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGat                                                                                                  <  1:1347284/150‑1 (MQ=255)
                                                     gAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGGCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGaaa                                                                                                 >  1:1121452/1‑148 (MQ=255)
                                                     gAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatc                                                                                                 <  2:315898/150‑1 (MQ=255)
                                                     gAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGata                                                                                                 >  1:447277/1‑149 (MQ=255)
                                                      aaaaaaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatca                                                                                                <  1:1457940/150‑1 (MQ=255)
                                                       aaaaaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCAGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcat                                                                                               >  2:1149433/1‑150 (MQ=255)
                                                          aaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCg                                                                                            <  2:1736952/150‑1 (MQ=255)
                                                            ttACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGc                                                                                          <  1:24062/150‑1 (MQ=255)
                                                             tACTTTGACTTGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCa                                                                                         <  2:864107/150‑1 (MQ=255)
                                                             tACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCa                                                                                         <  1:558379/150‑1 (MQ=255)
                                                               cTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGGCATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCATGCCAGAACCGTAGGCAGGATCATCCGGCCac                                                                                       >  2:279291/1‑150 (MQ=255)
                                                               cTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                       >  2:344325/1‑150 (MQ=255)
                                                               cTTTGACGGGATAATCGTAAACAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAAAAGTCCCCAGTCCAGAACCGTATGCAGGATCATCAGGCaaa                                                                                       >  2:1285099/1‑149 (MQ=38)
                                                                 ttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAacac                                                                                     <  1:431547/150‑1 (MQ=255)
                                                                  tGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACg                                                                                    <  2:679515/150‑1 (MQ=255)
                                                                   gACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt                                                                                   >  1:1552798/1‑150 (MQ=255)
                                                                     cGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTa                                                                                 <  2:227869/150‑1 (MQ=255)
                                                                         aTAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTgg                                                                             <  2:1682006/150‑1 (MQ=255)
                                                                          tAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGTGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGc                                                                            <  1:796796/150‑1 (MQ=255)
                                                                          tAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGc                                                                            <  1:1004922/150‑1 (MQ=255)
                                                                           aaTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCa                                                                           >  2:489514/1‑150 (MQ=255)
                                                                                    cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCTTAGGCAGGATCATCCGGCACCACGTTATTGGCAATCTGCggg                                                                  >  2:969038/1‑148 (MQ=255)
                                                                                    cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                                  <  1:758539/150‑1 (MQ=255)
                                                                                    cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                                  <  2:1053454/150‑1 (MQ=255)
                                                                                    aaaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                                  <  2:376605/149‑1 (MQ=255)
                                                                                      aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGaa                                                                >  1:783382/1‑150 (MQ=255)
                                                                                      aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGAGGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCCGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGaa                                                                <  2:164186/150‑1 (MQ=255)
                                                                                           aTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                           >  2:142570/1‑150 (MQ=255)
                                                                                            tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTg                                                          <  1:1655109/150‑1 (MQ=255)
                                                                                            tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTg                                                          <  1:1089945/150‑1 (MQ=255)
                                                                                             ttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGt                                                         <  1:1069472/150‑1 (MQ=255)
                                                                                              ttgTTTGATTTTTAGGACGCACGCCACATAAGACATTCAGCGCCTGATGCGACGCGTGACGCGTCTTATCATGCCTACAAGTCCCCATGCCAGAACCGTAGGCAGGATCATCCGGCAACCCGTTATTGGCAATCTGCGTGAATGTTTGTc                                                        <  1:652258/148‑1 (MQ=38)
                                                                                                gTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc                                                      <  1:287846/150‑1 (MQ=255)
                                                                                                 tttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAcc                                                     <  1:1422177/150‑1 (MQ=255)
                                                                                                     aTTTGTAGGCCGCACGCCACATCCGACATTCAGCTCCGGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTg                                                 <  1:771030/150‑1 (MQ=255)
                                                                                                     aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCTGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTg                                                 <  2:1212307/150‑1 (MQ=255)
                                                                                                         gTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTc                                             >  2:1498483/1‑150 (MQ=255)
                                                                                                                    gCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACtt                                  <  1:1267080/150‑1 (MQ=255)
                                                                                                                      cacaTCCGACATTCAGCGCCTGATGCGACGCTTGACCCGTCTTATCAGGCCTACAAGTCCACAGGCCAGAACCGTAGGCAGGATCATCAGGCAACACCTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACATTc                                >  2:798720/1‑150 (MQ=39)
                                                                                                                       acaTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCGCCAGTGCCTCCAGCACCACTTTcc                               <  1:1012742/150‑1 (MQ=255)
                                                                                                                       acaTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTcc                               <  1:1221432/150‑1 (MQ=255)
                                                                                                                       acaTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTcc                               <  2:696957/150‑1 (MQ=255)
                                                                                                                        caTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCa                              <  1:1459357/150‑1 (MQ=255)
                                                                                                                         aTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCCGGGCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCTTGAATGTTTGTCACCAGTGCCTCCCGGACCACTTTCCAg                             >  2:1200119/1‑150 (MQ=39)
                                                                                                                         aTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAg                             <  2:1552798/150‑1 (MQ=255)
                                                                                                                         aTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAg                             <  2:1091884/150‑1 (MQ=255)
                                                                                                                          tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGt                            <  1:1386626/150‑1 (MQ=255)
                                                                                                                           ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtc                           >  1:1163282/1‑150 (MQ=255)
                                                                                                                            cGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGGTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtcc                          >  2:1293868/1‑149 (MQ=255)
                                                                                                                               cATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAATTCCCCAGGCCAGAACCGTAGGCTGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCCCCACTTTCCAGtcatca                       >  1:1591966/1‑150 (MQ=255)
                                                                                                                                  tCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGACAGAACCGAAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTTAGTGAATGTTTGTCCCCAGTGCCTCCAGCACCACTTTCCAGTCAACAAcc                    >  2:1386868/1‑150 (MQ=38)
                                                                                                                                   cAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCa                   >  1:1179692/1‑150 (MQ=255)
                                                                                                                                    taggcCTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGGAACACGTTATTGGCAATCTGCGGGAATGTTTGTCACCAGTGCCTCCAGCACGACTTTCCAGTCATCAAcccc                  >  2:350098/4‑148 (MQ=37)
                                                                                                                                      cgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGc                <  1:1297289/150‑1 (MQ=255)
                                                                                                                                        ccTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCa              >  2:98794/1‑150 (MQ=255)
                                                                                                                                          tGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAAc            <  2:28291/150‑1 (MQ=255)
                                                                                                                                          tGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAAc            <  1:1498483/150‑1 (MQ=255)
                                                                                                                                           gATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACa           >  1:478590/1‑150 (MQ=255)
                                                                                                                                             tGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATc         <  2:463704/150‑1 (MQ=255)
                                                                                                                                               cGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTg       <  1:1738811/150‑1 (MQ=255)
                                                                                                                                                 aCGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGcc     <  2:589777/150‑1 (MQ=255)
                                                                                                                                                  cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGCCt    >  2:697737/1‑150 (MQ=255)
                                                                                                                                                    cTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACCCGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCACCATCTGCCtgt  >  1:684667/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGCCTGT  >  NC_000913/2894703‑2895000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: