Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 329,521 C→A 6.6% T20N (ACC→AAC)  betT → choline transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913329,5210CA 6.6% 302.8 / 6.3 98T20N (ACC→AAC) betTcholine transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base C (48/43);  new base A (5/2);  total (53/45)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

AAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATATTGTCTTTGTG  >  NC_000913/329372‑329669
                                                                                                                                                     |                                                                                                                                                    
aaGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTacac                                                                                                                                                      <  2:490213/150‑1 (MQ=255)
 aGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACAcc                                                                                                                                                     >  1:148669/1‑150 (MQ=255)
 aGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACAcc                                                                                                                                                     >  1:1359927/1‑150 (MQ=255)
      ttATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTccgc                                                                                                                                                >  1:373118/1‑150 (MQ=255)
        attgattgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTccgccg                                                                                                                                              <  1:1379946/150‑1 (MQ=255)
         ttgattgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCgg                                                                                                                                             <  2:574420/150‑1 (MQ=255)
            attgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACTCAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACt                                                                                                                                          >  2:1014601/1‑150 (MQ=255)
            attgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACATTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACt                                                                                                                                          >  2:937540/1‑150 (MQ=255)
            attgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTATAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACt                                                                                                                                          <  2:56470/150‑1 (MQ=255)
               ggACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAt                                                                                                                                       >  2:1510095/1‑150 (MQ=255)
                             aaaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTccc                                                                                                                         <  1:192713/150‑1 (MQ=255)
                               aTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTCCCTg                                                                                                                       <  1:759649/150‑1 (MQ=18)
                                  tgtCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACa                                                                                                                    <  1:207693/150‑1 (MQ=255)
                                  tgtCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACa                                                                                                                    <  2:740926/150‑1 (MQ=255)
                                     cTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACg                                                                                                                 <  2:198405/150‑1 (MQ=255)
                                       tAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGTAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGAt                                                                                                               <  2:1671314/150‑1 (MQ=255)
                                        aaTTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAACTGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATc                                                                                                              <  1:1467379/150‑1 (MQ=255)
                                            gTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGt                                                                                                          >  2:1291923/1‑150 (MQ=255)
                                            gTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGt                                                                                                          >  2:1700880/1‑150 (MQ=255)
                                                cGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTcg                                                                                                      >  2:686129/1‑150 (MQ=255)
                                                  aaTTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACGCCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTcgcg                                                                                                    >  2:171487/1‑150 (MQ=255)
                                                  aaTTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTcgcg                                                                                                    <  1:1761414/150‑1 (MQ=255)
                                                    tttGATTTTAAAGAGTAACAATAACAGTGGGGATACTGGATGACAGACCTGTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGATTTCCACCTCCGCCGGACTGAGTTTGTTCGTTTCCCTGACAACGATCCTGTTTCGAGAc                                                                                                  >  2:1069995/1‑150 (MQ=255)
                                                      tGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACtt                                                                                                >  1:1465208/1‑150 (MQ=255)
                                                           ttAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGGGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCgg                                                                                           <  1:1190485/150‑1 (MQ=255)
                                                            tAAATAGTAACATTAACAGTGGTGATTCTGGATGACAGACCTTTCACACAGCAGGTAAAAGGACAAAATCAATCCGGTGGTTTTTTACACCTCCGCAGGACTGATTTTGTTGTTTTCCCTGACAACGATGCTGTTTCGCGACTTCTCGGc                                                                                          <  1:771120/150‑1 (MQ=255)
                                                            tAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGc                                                                                          <  1:160355/150‑1 (MQ=255)
                                                              aaTAGTAACAATAACAGTGGGGATACTGGATGACAGCCCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGccc                                                                                        >  1:293045/1‑150 (MQ=255)
                                                                  gTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCtgtg                                                                                    >  2:11162/1‑150 (MQ=255)
                                                                  gTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCtgtg                                                                                    <  1:1319562/150‑1 (MQ=255)
                                                                  gTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCtgtg                                                                                    <  1:140020/150‑1 (MQ=255)
                                                                   tAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTgg                                                                                   <  2:107130/150‑1 (MQ=255)
                                                                    aaCAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGa                                                                                  >  1:316885/1‑150 (MQ=255)
                                                                     aCAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGAt                                                                                 >  1:1787102/1‑150 (MQ=255)
                                                                     aCAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGAt                                                                                 >  2:1130195/1‑150 (MQ=255)
                                                                      cAATAAGAGTGGGGATACTGGATGACAGACCTTTCACACCGCAGGGAAAAGGTCAAACTCATTGCGGGGGTGTGTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGAtt                                                                                <  1:1125251/150‑1 (MQ=255)
                                                                      cAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGAtt                                                                                >  1:103010/1‑150 (MQ=255)
                                                                        aTAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCTACTTCTCGGCCCTGTGGATTgg                                                                              >  2:1088834/1‑150 (MQ=255)
                                                                         tAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGc                                                                             >  2:679134/1‑150 (MQ=255)
                                                                           aCAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCg                                                                           >  2:1138603/1‑150 (MQ=255)
                                                                                ggggATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGc                                                                      >  2:65253/1‑150 (MQ=255)
                                                                                  ggATACTGGATGACAGACCTTTCACCCAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTg                                                                    <  2:104114/150‑1 (MQ=255)
                                                                                  ggATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTg                                                                    >  2:1673658/1‑150 (MQ=255)
                                                                                  ggATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTg                                                                    <  2:1547930/150‑1 (MQ=255)
                                                                                  ggATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTg                                                                    <  2:908603/150‑1 (MQ=255)
                                                                                   gATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTgg                                                                   <  2:499066/150‑1 (MQ=255)
                                                                                   gATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTgg                                                                   <  1:1717327/150‑1 (MQ=255)
                                                                                      aCTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACt                                                                >  1:23389/1‑150 (MQ=255)
                                                                                       cTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTg                                                               >  2:460068/1‑150 (MQ=255)
                                                                                       cTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTg                                                               >  1:1580096/1‑150 (MQ=255)
                                                                                        tcgATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGTCCCTGTGGATTGGCCGCACGCTGGACTgg                                                              <  1:1146135/148‑1 (MQ=255)
                                                                                           aTGACAGAACTTTCACACATCAGGGAAAAGGACAATATCAATCCGGCGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGtt                                                           <  2:61289/150‑1 (MQ=255)
                                                                                             gACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTc                                                         >  1:285931/1‑150 (MQ=255)
                                                                                             gACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTc                                                         >  2:1767714/1‑150 (MQ=255)
                                                                                              aCAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCt                                                        <  2:1683555/150‑1 (MQ=255)
                                                                                              aCAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCt                                                        >  2:209574/1‑150 (MQ=255)
                                                                                               aaGACCTTTCACACAGCAGTGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTa                                                       <  2:841906/149‑1 (MQ=255)
                                                                                                 gACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACCCCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTaaa                                                     <  2:1638552/150‑1 (MQ=255)
                                                                                                 gACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTaaa                                                     >  1:1454141/1‑150 (MQ=255)
                                                                                                   ccTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAAc                                                   >  2:1481427/1‑150 (MQ=255)
                                                                                                    cTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAAcc                                                  <  1:189568/150‑1 (MQ=255)
                                                                                                     tttCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACTACGATCCGGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCt                                                 >  2:1180332/1‑150 (MQ=255)
                                                                                                     tttCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAGTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGCCTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCt                                                 >  2:298014/1‑150 (MQ=255)
                                                                                                        cacacaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCg                                              >  1:524937/1‑150 (MQ=255)
                                                                                                          cacaGCTGGGAAAAGGACAAAATGAATCCGGTGGTGTTTTACACCTCCGCCGGTCTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGt                                            <  1:541960/150‑1 (MQ=255)
                                                                                                          cacaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGt                                            >  1:550879/1‑150 (MQ=255)
                                                                                                          cacaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGt                                            <  1:1272023/150‑1 (MQ=255)
                                                                                                            cagcagGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTg                                          >  2:21525/1‑150 (MQ=255)
                                                                                                            cagcagGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTg                                          >  2:832043/1‑150 (MQ=255)
                                                                                                              tcagGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTCCCTGACAACGATCCTGTTTCGCGCCTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGt                                        >  1:1228500/2‑150 (MQ=17)
                                                                                                                   gAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGGTTTCACTGACAACTATCCTGTTTCGCGACTTCTCGCCCCTGTGGAATGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGGACGAt                                   >  1:1676573/1‑150 (MQ=255)
                                                                                                                      aacaaCAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACATTCGGTTGGTACTATctg                                >  1:123438/5‑150 (MQ=21)
                                                                                                                       aGGACAAAATCCATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctgc                               <  1:523421/150‑1 (MQ=255)
                                                                                                                        ggACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCGTCGGTTGGTACTATctgct                              >  2:1172683/1‑150 (MQ=255)
                                                                                                                        caaCAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctgct                              >  2:927885/3‑150 (MQ=25)
                                                                                                                         aaCAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctgctg                             >  1:898813/2‑150 (MQ=25)
                                                                                                                          aCAAAATCAATCCGGTGGTGTGTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTgg                            <  1:1669110/150‑1 (MQ=255)
                                                                                                                           cAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggc                           <  1:1510095/150‑1 (MQ=255)
                                                                                                                           cAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTATCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACAGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggc                           >  2:190094/1‑150 (MQ=255)
                                                                                                                            aaaaTCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcg                          >  2:763351/1‑150 (MQ=255)
                                                                                                                             aaaTCAGTCCGGCGGAGGTGTAAAACACCACCGGGTTGATTTTGTCCTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcgg                         >  1:1004749/1‑150 (MQ=25)
                                                                                                                              aatcattcCGGTGGTGTTTTACACCGCCGCGGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATGGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcggc                        <  1:183270/150‑1 (MQ=255)
                                                                                                                              aatcaatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcggc                        <  1:1168687/150‑1 (MQ=255)
                                                                                                                              aatcaatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcggc                        <  1:768749/150‑1 (MQ=255)
                                                                                                                              aatcaatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTggcggc                        >  1:1311594/1‑150 (MQ=255)
                                                                                                                                 caatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAAc                     >  2:328258/1‑150 (MQ=255)
                                                                                                                                  aatcCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACg                    >  2:64552/1‑150 (MQ=255)
                                                                                                                                      ctgttgtgtTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGctct                <  2:148669/145‑1 (MQ=255)
                                                                                                                                      cGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGctct                >  2:1510960/1‑150 (MQ=255)
                                                                                                                                      cGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGctct                <  1:689296/150‑1 (MQ=255)
                                                                                                                                       ggtggtGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCta               <  1:318325/150‑1 (MQ=255)
                                                                                                                                        gtggtgTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCtat              >  1:327489/1‑150 (MQ=255)
                                                                                                                                            tgtTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATAttt          >  1:454777/1‑149 (MQ=255)
                                                                                                                                            tgtTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATAttt          >  2:60367/1‑149 (MQ=255)
                                                                                                                                            tgtTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATATTg          <  1:679134/150‑1 (MQ=255)
                                                                                                                                             gtTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATATTGt         <  1:65253/150‑1 (MQ=255)
                                                                                                                                               tttACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATATTGTCt       <  2:1102598/150‑1 (MQ=255)
                                                                                                                                                  acacCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATATTGTCTTtg    >  1:366777/1‑150 (MQ=255)
                                                                                                                                                   cacCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATATTGTCTTtgt   >  2:927997/1‑150 (MQ=255)
                                                                                                                                                    acCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATATTGTCTTtgtg  <  1:1014601/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCTGGCGGCAACGCTCTATATTGTCTTTGTG  >  NC_000913/329372‑329669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: