Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,918,022 A→G 5.9% intergenic (+209/+23) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,918,0220AG 5.9% 351.9 / 9.0 120intergenic (+209/+23)barA/gudDhybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base A (61/49);  new base G (2/5);  total (64/56)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 120 total aligned reads displayed.

CTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCAC  >  NC_000913/2917878‑2918167
                                                                                                                                                 |                                                                                                                                                 
cTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGcc                                                                                                                                              <  2:1492978/150‑1 (MQ=255)
cTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGcc                                                                                                                                              <  1:802451/150‑1 (MQ=255)
   tttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggt                                                                                                                                           >  1:443609/8‑150 (MQ=39)
   tgtTTATGCAGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggt                                                                                                                                           <  1:1125865/150‑1 (MQ=38)
    ttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACACCAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggtt                                                                                                                                          >  2:121230/7‑150 (MQ=39)
      ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                        <  2:1781656/150‑2 (MQ=255)
      ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                        <  1:230647/150‑2 (MQ=255)
      ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggtttt                                                                                                                                        >  1:1499337/1‑150 (MQ=255)
       tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttg                                                                                                                                       <  1:802703/150‑1 (MQ=255)
       tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggatttg                                                                                                                                       >  2:1663242/1‑150 (MQ=255)
        aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttgag                                                                                                                                      <  2:1492587/150‑4 (MQ=38)
          tccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGCAGCCggtttgagac                                                                                                                                    <  1:1686080/149‑6 (MQ=38)
          gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAAGGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggtt                                                                                                                                    >  1:450374/1‑150 (MQ=255)
          gCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggtt                                                                                                                                    >  1:428547/1‑150 (MQ=255)
           ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggttt                                                                                                                                   <  2:881034/150‑1 (MQ=255)
             ggataaggcgTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGGTTTTc                                                                                                                                 >  1:366452/7‑150 (MQ=255)
              gATGCGGAGTTAACGCCTTATCCGGCCTACAAAAACAATTAAATTCAATGAATTTAAAAAATGATTTAGACCTGATACATAGGACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTc                                                                                                                                 <  2:1539607/150‑1 (MQ=38)
               aTGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCATTGAATTAAAAAATGATGTAGACCTGATACATAGCACAAGAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTg                                                                                                                               <  2:126199/150‑1 (MQ=255)
               aTGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTg                                                                                                                               <  1:746967/150‑1 (MQ=255)
               aTGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTg                                                                                                                               <  1:1717997/150‑1 (MQ=255)
                 tcggcgTTAACTCGTTATCCGGCCTACATAACCACTTAAATTCAATTAATTAAAAAATGTTGTAGACCTGATACATCGCACAACAGGCTATTTTGCGTCTAGCATCAGTCTCAACCCGGCTCCAGATTGTGACGGTTTTGGTTTTCTGTc                                                                                                                             <  1:950412/149‑1 (MQ=255)
                 gcggcgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTc                                                                                                                             <  2:176055/150‑1 (MQ=255)
                  cggcgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCt                                                                                                                            >  2:962261/1‑150 (MQ=255)
                    gcgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTa                                                                                                                          >  2:1622191/1‑150 (MQ=255)
                       ttAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACg                                                                                                                       >  2:1733755/1‑150 (MQ=255)
                         aaCGCCTTATCCGGCCTACAAACCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                     >  2:1143581/1‑150 (MQ=255)
                         aaCGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                     >  1:684265/1‑150 (MQ=255)
                         aaCGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                     <  1:901398/150‑1 (MQ=255)
                              cTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                <  1:1326738/150‑1 (MQ=255)
                              cTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                <  2:1398068/150‑1 (MQ=255)
                                tATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAACACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGATCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCa                                                                                                              >  2:543591/1‑150 (MQ=255)
                                  tCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACg                                                                                                            >  2:630602/1‑150 (MQ=255)
                                   ccGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACgg                                                                                                           >  2:1476940/1‑150 (MQ=255)
                                   ccGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACgg                                                                                                           <  1:1263876/150‑1 (MQ=255)
                                       ccTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGgcgc                                                                                                       >  2:794828/1‑150 (MQ=255)
                                        cTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCt                                                                                                      >  1:764250/1‑150 (MQ=255)
                                         tACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACAATGCACGGGCGCtt                                                                                                     >  2:78147/1‑150 (MQ=255)
                                         tACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGCCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATTCACGGGCGCtt                                                                                                     >  2:1323562/1‑150 (MQ=255)
                                          aCAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTg                                                                                                    <  1:587266/150‑1 (MQ=255)
                                          aCAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTg                                                                                                    <  2:635113/150‑1 (MQ=255)
                                          aCAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTg                                                                                                    <  1:1492480/150‑1 (MQ=255)
                                          aCAAAAACAATTAAATTCACTGAATTAAAAAATGATGTAGACCGCCTACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTg                                                                                                    <  2:1154745/150‑1 (MQ=255)
                                            aaaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGtt                                                                                                  >  2:1398798/1‑150 (MQ=255)
                                             aaaCCAATTAAATTCAATTAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGGCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTa                                                                                                 <  2:248991/150‑1 (MQ=255)
                                             aaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTa                                                                                                 >  1:20618/1‑150 (MQ=255)
                                             aaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTa                                                                                                 <  1:625681/150‑1 (MQ=255)
                                              aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGTTACATAGCACAACAGGATATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCTGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCTCGGGCGCTTGTTAt                                                                                                <  1:826095/150‑1 (MQ=255)
                                              aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTAt                                                                                                <  2:1455475/150‑1 (MQ=255)
                                                      aaaTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACACGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTc                                                                                        <  1:47906/150‑1 (MQ=255)
                                                         ttCAATGAATTAAAAAATGATGTAGCCCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccag                                                                                     >  1:1137850/1‑150 (MQ=255)
                                                         ttCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGATCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTAGTTATCCAACGTccac                                                                                     >  2:1345519/1‑149 (MQ=255)
                                                         ttCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccag                                                                                     <  1:440556/150‑1 (MQ=255)
                                                           cAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagcc                                                                                   >  1:682670/1‑150 (MQ=255)
                                                            aaTGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagcca                                                                                  >  2:805058/1‑150 (MQ=255)
                                                             aTGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagccag                                                                                 <  1:1114697/150‑1 (MQ=255)
                                                                aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGAGAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGaa                                                                              >  1:659180/1‑150 (MQ=255)
                                                                   ttAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCATGTTATCGAACGTCCAGCCAGGAATc                                                                            >  1:1449994/1‑150 (MQ=255)
                                                                   ttAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGGCTTAACGCACCATGCACGTCGGCTGGTTATCGAACGTCAAGCAAGTAATc                                                                            >  2:275071/1‑150 (MQ=255)
                                                                     aaaaaaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAg                                                                          <  2:224967/150‑1 (MQ=255)
                                                                      aaaaaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGa                                                                         >  2:467925/1‑150 (MQ=255)
                                                                      aaaaaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGa                                                                         <  1:962261/150‑1 (MQ=255)
                                                                       aaaaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCCCTTGTTATCGAACGTCCAGCCATGAATCAGAt                                                                        >  1:1151236/1‑150 (MQ=255)
                                                                          atgatgTAGACCTGATACATCGCTCAACCGGCTATTTTGCGTTTAGCATGAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACt                                                                     <  2:1218341/150‑1 (MQ=255)
                                                                            gatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGc                                                                   <  1:1368309/150‑1 (MQ=255)
                                                                            gatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGc                                                                   <  2:28858/150‑1 (MQ=255)
                                                                                tAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTc                                                               >  1:980085/1‑150 (MQ=255)
                                                                                 aGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATTGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTcc                                                              >  2:586963/1‑150 (MQ=255)
                                                                                 aGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTcc                                                              >  2:1484036/1‑150 (MQ=255)
                                                                                   aCCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATCGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCca                                                            <  1:51476/150‑1 (MQ=255)
                                                                                   aCCTGATACATAGCACAAAAGGATATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCca                                                            <  2:59268/150‑1 (MQ=255)
                                                                                       gATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGTATTCCcatcgc                                                        <  1:530012/150‑1 (MQ=255)
                                                                                       gATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgc                                                        <  2:1673627/150‑1 (MQ=255)
                                                                                           cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgcatcg                                                    >  1:1369219/1‑150 (MQ=255)
                                                                                             tAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGCTAGAGCCGGTTTTGGTTTTCTGTCTTAACACACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTc                                                  >  1:733122/1‑150 (MQ=255)
                                                                                               gCACAACAGGCTATTTTGCGTTTAGCATCAGACTCAAACCTGCTCCAGATAGAGCCGGTTTTGGTTATCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGTAATCAGATACTGCATTCCCATCGCATCGTCAc                                                <  1:283511/150‑1 (MQ=255)
                                                                                                  caacaGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCCGCCCGGAATCGGTAACTGCCTTCCCATCGCATCGTcgagcg                                             >  2:1110186/1‑145 (MQ=255)
                                                                                                    acaGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCTCCATGCACGGGCGCTTGTTATCGACCGTCCCGCCAGGCATCAGATACTGCATTCCCATCGCATCGTCAcgcgag                                           >  1:273444/1‑148 (MQ=255)
                                                                                                      aGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGcc                                         <  2:684265/150‑1 (MQ=255)
                                                                                                       ggCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTTTCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCa                                        >  2:71638/1‑150 (MQ=255)
                                                                                                       ggCTATTTTGCGTTTAGCATCAGTCACAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCATAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCACAACGCATAGTCACGCGCGCCa                                        >  2:346487/1‑150 (MQ=255)
                                                                                                          tATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCCGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGc                                     >  2:1562950/1‑150 (MQ=255)
                                                                                                           aTTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGcc                                    <  1:827605/150‑1 (MQ=255)
                                                                                                           aTTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGcc                                    <  1:1712183/150‑1 (MQ=255)
                                                                                                            ttttGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGccc                                   <  2:428547/150‑1 (MQ=255)
                                                                                                                 cGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGtt                              >  1:378551/1‑150 (MQ=255)
                                                                                                                    ttAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCCCGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCt                           >  2:984049/1‑150 (MQ=255)
                                                                                                                     tAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTGTGTCTTAACGCACCATGCACGGGCGCGTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCACATCGCAGCGTCCCGCGCGCCAAGCCCGTGTTTCTg                          >  1:1439468/1‑150 (MQ=255)
                                                                                                                       gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                        <  2:764250/150‑1 (MQ=255)
                                                                                                                             aaccaaaaCTGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCt                  >  2:964072/6‑150 (MQ=255)
                                                                                                                               ctcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCa                >  1:158664/1‑150 (MQ=255)
                                                                                                                               ctcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAAAGTCCAGCCAGGAATCAGATACTGCAATACCAGCGCATCGTCACGCGCGCCAAGGCCGTGGTTCTGATACAGCtaa                >  1:1355555/1‑148 (MQ=255)
                                                                                                                                tcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAt               <  1:324706/150‑1 (MQ=255)
                                                                                                                                tcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAt               >  2:575219/1‑150 (MQ=255)
                                                                                                                                 cAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCATGCCCGTGTTTCTGATACAGCTCagg              >  2:69630/1‑148 (MQ=255)
                                                                                                                                  aaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGCTACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGAGTTTCTGATACAGCTCATgg             >  2:91807/1‑150 (MQ=255)
                                                                                                                                  aaaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATgg             <  2:450374/150‑1 (MQ=255)
                                                                                                                                   aaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTTTCGAACGTCCCGCCAGGAATCAGATACTGCAGTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATggg            >  1:999280/1‑150 (MQ=255)
                                                                                                                                    aCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGAACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGCGACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGc           >  2:1720613/1‑150 (MQ=255)
                                                                                                                                           ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTACAGCCAGGAATCAGATACTTCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTtcatc    >  1:1165307/1‑150 (MQ=255)
                                                                                                                                             aGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCAc  >  1:442795/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCAC  >  NC_000913/2917878‑2918167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: