Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,931,836 T→G 5.4% intergenic (+26/+29) ygdG → / ← fucO Ssb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,931,8360TG 5.4% 259.0 / 4.0 98intergenic (+26/+29)ygdG/fucOSsb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (35/57);  new base G (4/2);  total (39/59)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 105 total aligned reads displayed.

CGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCGTAC  >  NC_000913/2931687‑2931984
                                                                                                                                                     |                                                                                                                                                    
cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgt                                                                                                                                                      >  2:1175360/1‑150 (MQ=255)
  aaaaGTGGCGCAAAAAATTATAAACCCATAAAGTCATGGCGTTTCTGTTCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtcg                                                                                                                                                    <  2:939229/150‑1 (MQ=255)
  aaaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAACCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcggcg                                                                                                                                                    <  1:1510417/150‑1 (MQ=255)
  aaaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtcg                                                                                                                                                    <  2:6044/150‑1 (MQ=255)
    atgTGGCGCAAAAAATTAGAAACCCATAAAGAGATTGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACACCAATTGCGGTTGGTACGGTAACGGCTAGCCGGATACGCCGCAAACGTCGTa                                                                                                                                                  <  2:1800566/148‑1 (MQ=255)
    aaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTa                                                                                                                                                  <  2:1080435/150‑1 (MQ=255)
     aGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACAGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGAAACGCCGCAAAAGTAGTAt                                                                                                                                                 >  2:533148/1‑150 (MQ=255)
       gggCGCAAAAAATAAGAAACCCATAACGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTAGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATcc                                                                                                                                               <  2:347514/149‑1 (MQ=255)
         gcgcAAAAACTTACAAACCCAAAAAGAGATCGCGTTTCTGTGCCGATATATTGCCCTCTTACATACAGATTTGCTTATCGACGGAAATTTACAGCAATTGCGGTTGGAACGTTAACGGCTAGCCGGATACGCCGCAAACGTCGTATCCgg                                                                                                                                             <  2:1148037/150‑1 (MQ=255)
         gcgcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAAATGCGGTTGGTACGGTAACGGCGAGCCGGATAAGCCGCAAACGTCGAATCCgg                                                                                                                                             >  1:1123584/1‑150 (MQ=255)
           gcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGACGTTTGCGGCGTATCCGGct                                                                                                                                           <  2:597282/150‑2 (MQ=255)
            cAAAAAATTAGAAACCGATAAAGAGATGGCGTTTCTGTGCAGCGATATTGCCCGCTTACAAACCTATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCAt                                                                                                                                          <  2:266831/150‑1 (MQ=255)
            cAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACTGTAACTGCGAGACTGATAAGCCGCACACGTCATATCCGGAAt                                                                                                                                          >  2:1253364/1‑150 (MQ=255)
             aaaaaaTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCAtt                                                                                                                                         <  2:1566842/150‑1 (MQ=255)
              aaaaaTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTACCGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCAttt                                                                                                                                        >  1:246426/1‑149 (MQ=255)
                aaaTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATc                                                                                                                                      <  1:1405311/150‑1 (MQ=255)
                aaaTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATc                                                                                                                                      <  2:1236562/150‑1 (MQ=255)
                  aTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATcac                                                                                                                                    <  1:1198145/150‑1 (MQ=255)
                   ttAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATcaca                                                                                                                                   <  2:1555203/150‑1 (MQ=255)
                        aaCCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAACCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTCGTACGGTAACGGCGAGCCGGATAAGGCCGAAAAGTCGGATACGGCATTATAACAtacgc                                                                                                                              >  1:1788074/1‑146 (MQ=255)
                             aTAAAGAGATGGCGTTTCTGAGCAGCGATATTGACAGCTTACAAAACGATTTGAATATCGACGGCAATTTACAGCAATTGCGGTTTGTAAGGTAACGGCGAGCCGGATACGCAGCAATCGTCGTATACTGCATTATCAAATCAGCGCAtt                                                                                                                         >  2:1293662/1‑150 (MQ=255)
                              tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACTGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAttt                                                                                                                        >  2:1110235/1‑150 (MQ=255)
                              tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAttt                                                                                                                        >  1:1048981/1‑150 (MQ=255)
                               aaaGAGATGGCGTTTCTGTGCCTAGATATTGCCCGCTTACAAACCGATATTAATATAGACGGAAATTTACAGCAATTGCGGTTGGTTCGGTTACGGCGAGCCGGATACGCCGCATAAGTCGAATCCGGAATTATCACATCAGCGCATTTa                                                                                                                       <  2:939083/150‑1 (MQ=255)
                               aaaGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTa                                                                                                                       <  2:246533/150‑1 (MQ=255)
                                aaGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAc                                                                                                                      <  1:939883/150‑1 (MQ=255)
                                  gagaTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCa                                                                                                                    <  1:757050/150‑1 (MQ=255)
                                       ggCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCgg                                                                                                               <  1:533148/150‑1 (MQ=255)
                                       ggCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCgg                                                                                                               <  1:1166030/150‑1 (MQ=255)
                                            ttCTGTGCCGCGATATTGCCCGCTTACAACCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATgg                                                                                                          <  1:1050367/150‑1 (MQ=255)
                                            ttCTGTGCCGCGATATTGCCCGCTGACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATgg                                                                                                          <  1:564796/150‑1 (MQ=255)
                                              cTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTTTCCCGCATTATAACATAAGCGCATTTTCCATGCGGGATGGtt                                                                                                        >  2:360313/1‑149 (MQ=255)
                                               tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATAACCCGCAAACGTCGTATAACGGATTTTAACAAAAGCGCATTTTACAAGCGGTATTGtta                                                                                                       >  2:1555491/1‑148 (MQ=255)
                                                 tgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAg                                                                                                     >  1:57763/1‑150 (MQ=255)
                                                  gCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGGTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGc                                                                                                    <  2:1282988/150‑1 (MQ=255)
                                                   ccGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGct                                                                                                   >  1:246964/1‑150 (MQ=255)
                                                    cgcgATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGctc                                                                                                  <  1:1305941/150‑1 (MQ=255)
                                                    cgcgATAATGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTGGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGctc                                                                                                  <  1:943235/150‑1 (MQ=255)
                                                     gcgATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGctct                                                                                                 <  1:522408/150‑1 (MQ=255)
                                                         tatTGCCTGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACaa                                                                                             <  1:685445/150‑1 (MQ=255)
                                                             gCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTTTCACATCAGCGCATTTACCAGGCGGTATGGGAAAGCGATACAATATc                                                                                         >  2:1683981/1‑150 (MQ=255)
                                                              cccGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATcc                                                                                        <  2:1530810/150‑1 (MQ=255)
                                                                      cAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGGCGTATACGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGt                                                                                >  1:642180/1‑150 (MQ=255)
                                                                       aaaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGCTAAGCCGCAAACGTCGTATACGGCATTATCAAAACAGCGCATTTTCCAGGCGGTATGGTTAAGCTCTTAAATTTACTCAAGCGtt                                                                               >  2:532606/1‑150 (MQ=255)
                                                                        aaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTg                                                                              >  1:421174/1‑150 (MQ=255)
                                                                        aaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGAAAACGTCTTATCAGGCATTATCACATCAGCGCATTTACAAGGCGGTAAGGTAAAGCTCTACAATATACTCAAGCGttt                                                                              >  1:989482/1‑149 (MQ=255)
                                                                         aCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGCTAAGCCGCAAACGTAGTATGCGGCATTATCACATAAGCGCATTTACCAGGCGGTATGGTAAAGCTCTAAAATTTACTCAAGCGTTgg                                                                             >  2:501586/1‑149 (MQ=255)
                                                                          ccGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCt                                                                            <  2:1082918/150‑1 (MQ=255)
                                                                          ccGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCt                                                                            <  2:925615/150‑1 (MQ=255)
                                                                             aTTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCa                                                                         <  1:501824/150‑1 (MQ=255)
                                                                                  cATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTAACACATCAGCGCAATTACCAGGCGGTATGGTAAAGCTCTACAATATACTCAAGCGTTGCTTCACGCgg                                                                    >  1:1308714/1‑150 (MQ=255)
                                                                                   atatCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCggg                                                                   <  2:752699/150‑1 (MQ=255)
                                                                                   atatCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCggg                                                                   <  2:396982/150‑1 (MQ=255)
                                                                                      tCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGGTACCCCGCAAACGTAGTATCCCGCAATATAACATCAGCGCATTTTCCAGGCGGGTTGGGAAAGCTCTACAAAATCCTGAAGCCTTGCTTCCCGCGGGttt                                                                >  2:660100/1‑149 (MQ=255)
                                                                                          cGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCAc                                                            >  2:50899/1‑150 (MQ=255)
                                                                                          cGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATAAGCCCCAAACGTCGGATCCGGCATTATCACATCAGCGCAGTTACCAGGCGGTATGGTAAAGCTCTACAATATACTCAAGCGTTGCATCACGCGGGTTGCCAc                                                            >  2:635308/1‑150 (MQ=255)
                                                                                           ggCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCAcc                                                           <  1:981942/150‑1 (MQ=255)
                                                                                           ggCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCAcc                                                           <  1:406292/150‑1 (MQ=255)
                                                                                           ggCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCAcc                                                           <  1:1365612/150‑1 (MQ=255)
                                                                                             cAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCgg                                                         <  1:541467/150‑1 (MQ=255)
                                                                                               aTTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTa                                                       <  1:1505948/150‑1 (MQ=255)
                                                                                                tttACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATAAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTAc                                                      >  1:1105785/1‑150 (MQ=255)
                                                                                                 ttACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATAAGCCGCAAAAGTCGTATCCGGCATTATAACAAAAGCCCAATTACCAGGCCGTATGGTAAAGCACTAAAATATCCACAATCGTTGCTTAACGCGGGTTGACAACCGTACa                                                     >  2:1825619/1‑150 (MQ=255)
                                                                                                   aCAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCCAGCGGGTTGGCCCCCGTACaaa                                                   >  2:693000/1‑150 (MQ=255)
                                                                                                    cAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAAc                                                  >  1:1034975/1‑150 (MQ=255)
                                                                                                    cAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAATTAACAGGCGGTATGGTAAAGCTATACAATATCCTAAAGCGTTGCGTAACGCGGGTTTCCACCGGTACaaaa                                                  >  2:518730/1‑149 (MQ=255)
                                                                                                     aGCAATTGCGGTTGGTACGGGAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTCTCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGGTGCCACCGGTACAAAca                                                 <  2:300301/150‑1 (MQ=255)
                                                                                                       cAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAAcatc                                               >  1:935452/1‑150 (MQ=255)
                                                                                                           tGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATcc                                           <  1:1681491/150‑1 (MQ=255)
                                                                                                               gTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTg                                       <  2:585767/150‑1 (MQ=255)
                                                                                                               gTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAAGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTg                                       <  2:1123584/150‑1 (MQ=255)
                                                                                                                  ggTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTgccg                                    <  1:518730/150‑1 (MQ=255)
                                                                                                                      cGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTg                                >  1:1030566/1‑150 (MQ=255)
                                                                                                                          aaCGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGcc                            <  1:606817/150‑1 (MQ=255)
                                                                                                                            cGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGTGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAg                          <  2:1814827/150‑1 (MQ=255)
                                                                                                                            cGGCGAGCCGGATACGCAGCAAACGTCATATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAg                          <  2:1701890/150‑1 (MQ=255)
                                                                                                                                gAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGcc                      >  2:215908/1‑150 (MQ=255)
                                                                                                                                 aGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCg                     <  1:635804/150‑1 (MQ=255)
                                                                                                                                  gCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCCTTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCAACCGGTCGAAACATCATTCAGTGCCGCCTGCGCCAGTGCCgg                    >  2:1125934/1‑150 (MQ=255)
                                                                                                                                  gCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCgg                    <  2:540575/150‑1 (MQ=255)
                                                                                                                                   ccGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGa                   <  2:582791/150‑1 (MQ=255)
                                                                                                                                   ccGGATACGACGTTTGCGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGa                   >  1:1201326/1‑150 (MQ=255)
                                                                                                                                   ccGGATACGACGTTTGCGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGa                   >  1:1263328/1‑150 (MQ=255)
                                                                                                                                   ccGGATACGACGTTTGCGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGa                   >  1:36037/1‑150 (MQ=255)
                                                                                                                                    cGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGaa                  >  2:1549624/1‑150 (MQ=255)
                                                                                                                                     ggATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAAt                 >  1:40483/1‑150 (MQ=255)
                                                                                                                                     ggATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAAt                 <  1:1300996/150‑1 (MQ=255)
                                                                                                                                      gATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACTATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg                <  2:441445/150‑1 (MQ=255)
                                                                                                                                      gATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg                <  2:804176/150‑1 (MQ=255)
                                                                                                                                      gATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg                <  2:246586/150‑1 (MQ=255)
                                                                                                                                        tACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCCGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTc              >  2:578739/1‑150 (MQ=255)
                                                                                                                                         aCGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCt             <  1:455984/150‑1 (MQ=255)
                                                                                                                                            ccgcAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTcc          >  1:1035549/1‑150 (MQ=255)
                                                                                                                                            ccgcAAACGTCGTATCCGGCATTATCACATCAGCGCAATTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCATCACGCGGGTTGCCACCGGTACAAACATCATCCAGAGCCGCCTGCGCCAGTGCCGGAATGTCTTcc          >  2:741786/1‑150 (MQ=255)
                                                                                                                                             cgcAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCt         <  2:1239318/150‑1 (MQ=255)
                                                                                                                                             cgcAAACGTCGTATCCGGAATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGGTTCACGCGGGGTGCCACCGGTACAATCATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCt         <  2:1221755/150‑1 (MQ=255)
                                                                                                                                               cAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTg       <  2:246964/150‑1 (MQ=255)
                                                                                                                                                aaaCGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGc      <  1:1570031/150‑1 (MQ=255)
                                                                                                                                                    gtcgtATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCGTac  >  1:1377934/1‑150 (MQ=255)
                                                                                                                                                    gtcgtATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCGTac  >  2:1130735/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCGTAC  >  NC_000913/2931687‑2931984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: