Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,987,104 T→A 5.7% intergenic (+28/‑432) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,987,1040TA 5.7% 419.7 / 10.7 141intergenic (+28/‑432)yqeG/yqeHputative transporter/putative LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (62/71);  new base A (2/6);  total (64/77)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

Only 100 of 143 total aligned reads displayed.

ATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTA  >  NC_000913/2986960‑2987253
                                                                                                                                                |                                                                                                                                                     
atcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAt                                                                                                                                                  <  1:1550885/150‑1 (MQ=255)
 tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtt                                                                                                                                                 <  2:1421645/150‑1 (MQ=255)
   atGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTAGCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtttt                                                                                                                                               >  1:167412/1‑150 (MQ=255)
     gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCTGAAATCTGAATAATCTGATCGGGGGTATGCTGTGCGTATCTGTAATGTTCTTTAGCTAATTTCTGTTGTGGTTGGTTCAAAATTGCTACAATTTTGa                                                                                                                                             <  2:128551/150‑1 (MQ=255)
      ccTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGaa                                                                                                                                            >  1:388286/1‑150 (MQ=255)
      ccTACGCTGTCAACGAATCTCAACCCGGCGCTTAAACCCTGGAGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGaa                                                                                                                                            <  1:930362/150‑1 (MQ=255)
       cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGAAc                                                                                                                                           <  1:847988/150‑1 (MQ=255)
       cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGAAc                                                                                                                                           <  2:1671640/150‑1 (MQ=255)
       cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGAAc                                                                                                                                           <  2:1574394/150‑1 (MQ=255)
       cTACGCTGTCAACGTATCTCATCCCGGCGCTAAACCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTAACCTGTGCGTAGCGGTAATGTTCTTTAGCTAATACCAGTTGTGGATGGCTCAAAATTGTAGCAATTTTGAAc                                                                                                                                           <  2:1444696/150‑1 (MQ=255)
        tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGAAcc                                                                                                                                          <  1:636612/150‑1 (MQ=255)
        tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGAAcc                                                                                                                                          <  2:1760989/150‑1 (MQ=255)
        tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGAGTATCCTGTGCGGATCGGTATTGTTCTTTAGCTAATATCAGTTGTTGATGGTTCAAAATTGATACAATTTTGAAcc                                                                                                                                          >  2:88953/1‑150 (MQ=255)
         aCGCTGTCAACGTTTCTCATCCCGGTGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAcca                                                                                                                                         >  2:1211617/1‑150 (MQ=255)
            cTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattc                                                                                                                                      <  1:1825962/150‑1 (MQ=255)
            cTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattc                                                                                                                                      >  2:1168090/1‑150 (MQ=255)
               tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattccat                                                                                                                                   >  1:1324287/1‑150 (MQ=255)
                 aaCGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCAttt                                                                                                                                 >  2:144281/1‑150 (MQ=255)
                 aaCGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCAttt                                                                                                                                 >  2:977462/1‑150 (MQ=255)
                  aCGTATCTCATCCCGGCGCTTATACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCAtttt                                                                                                                                <  1:1294388/150‑1 (MQ=255)
                    gTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGAGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTc                                                                                                                              <  1:1573540/150‑1 (MQ=255)
                      aTCTCATCCCGGCTCTTTAACCCTGGCGTTCCATCGGAAATCTGATTAGGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGAGGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCat                                                                                                                            <  1:252285/150‑1 (MQ=255)
                      aTCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCat                                                                                                                            <  2:514803/150‑1 (MQ=255)
                       tctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCata                                                                                                                           >  2:477372/1‑150 (MQ=255)
                        ctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGGTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                                                                                          <  1:335223/150‑1 (MQ=255)
                         tcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataat                                                                                                                         >  1:1304783/1‑150 (MQ=255)
                         tcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataat                                                                                                                         >  2:917250/1‑150 (MQ=255)
                              ccGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCa                                                                                                                    <  1:1347809/150‑1 (MQ=255)
                              ccGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCa                                                                                                                    >  2:1497385/1‑150 (MQ=255)
                               cGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAt                                                                                                                   >  2:465348/1‑150 (MQ=255)
                                 tggcTTAAACCCTGGCGTTACATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAttt                                                                                                                 <  1:651836/148‑1 (MQ=255)
                                        aaCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGttatta                                                                                                          <  1:1404238/150‑1 (MQ=255)
                                         aCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTATCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTtattat                                                                                                         <  1:1803315/150‑1 (MQ=255)
                                          cccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTattatt                                                                                                        >  1:892126/1‑150 (MQ=255)
                                              ggCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAc                                                                                                    <  1:1069509/150‑1 (MQ=255)
                                              ggCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATAGGTAATGTTCTTTAGCTAATATCAGTTTTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAc                                                                                                    <  1:30953/150‑1 (MQ=255)
                                                 gTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCt                                                                                                 >  2:161143/1‑150 (MQ=255)
                                                  ttCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCTGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTa                                                                                                <  1:1065694/150‑1 (MQ=255)
                                                      aTCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCAc                                                                                            <  1:363315/150‑1 (MQ=255)
                                                      aTCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATAAc                                                                                            >  1:736769/1‑150 (MQ=255)
                                                      aTCGGAAATCTGATTACGCTGATCGGGGGTATCCTGTGCGGATCGGTAATGTTCTTAAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCAc                                                                                            <  2:301707/150‑1 (MQ=255)
                                                         ggAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtc                                                                                         >  1:157749/1‑150 (MQ=255)
                                                          gAAATCTGATTACGCTGATCGTGGGTATACTGTGCGTATCGGTAATGTTCTTTAGCTAATATCTGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATTACTtct                                                                                        <  1:118272/150‑1 (MQ=255)
                                                           aaaTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctc                                                                                       <  2:792412/150‑1 (MQ=255)
                                                           aaaTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctc                                                                                       <  2:538527/150‑1 (MQ=255)
                                                            aaTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctct                                                                                      >  2:420105/1‑150 (MQ=255)
                                                               cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                   <  2:203351/150‑1 (MQ=255)
                                                               cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATAGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                   <  1:1140436/150‑1 (MQ=255)
                                                                tGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTc                                                                                  >  2:833960/1‑150 (MQ=255)
                                                                 gATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                                                                                 <  2:1565448/150‑1 (MQ=255)
                                                                 gATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                                                                                 >  2:176214/1‑150 (MQ=255)
                                                                  aTTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCat                                                                                >  2:783860/1‑150 (MQ=255)
                                                                   ttACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATTGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCata                                                                               <  1:98962/150‑1 (MQ=255)
                                                                      cGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATcc                                                                            >  1:439788/1‑150 (MQ=255)
                                                                      cGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCAACCCTATCCATTATCTCGTCATATcc                                                                            >  1:1604136/1‑150 (MQ=255)
                                                                       gCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCt                                                                           <  1:176214/150‑1 (MQ=255)
                                                                        cTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                          >  2:1445181/1‑150 (MQ=255)
                                                                             cGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTATCTCGTCATATCCTAGtatt                                                                     >  1:1598714/1‑150 (MQ=255)
                                                                                    aTCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCGCCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtt                                                              <  2:1750155/150‑1 (MQ=255)
                                                                                     tcatctgCGTAACGGTAATGTTCTGTAGCTAATCTCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATATATCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtta                                                             <  2:1206064/145‑1 (MQ=255)
                                                                                        tgtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtc                                                                                         <  2:386650/119‑1 (MQ=255)
                                                                                         gtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAgttagtta                                                         <  2:1803121/150‑1 (MQ=255)
                                                                                          tgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAATATTGCTACAATTTTGAACCATTCCATTTTTCATATTATTCATTTGATATTATTCCACCCTATCACTTCTCTCGTCATATGCTTGTTTTATCAGTTAGTTaa                                                        <  2:660109/150‑1 (MQ=255)
                                                                                            cGTATCGGTAATGTTCTTTAGCTAATATCAGTTTTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATATTATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAac                                                      <  2:419507/150‑1 (MQ=255)
                                                                                            cGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCGACCCTATCACTTCTATCGTCATATCCTAGTATTATCAGTTAGTTaaaa                                                      >  1:142329/1‑149 (MQ=255)
                                                                                              tATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTATCGTCATATCCTATTATTATCAGTTAGTTAAacac                                                    >  1:59658/1‑150 (MQ=255)
                                                                                                tCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacacac                                                  <  1:1109752/150‑1 (MQ=255)
                                                                                                    tAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAag                                              >  2:319903/1‑150 (MQ=255)
                                                                                                       tGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAgaga                                           <  1:144281/150‑1 (MQ=255)
                                                                                                       tGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAgaga                                           <  1:977462/150‑1 (MQ=255)
                                                                                                        gTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGAt                                          <  2:1329629/150‑1 (MQ=255)
                                                                                                         ttCTTTAGCTAATATCAGTTGTGGATTGTTCAAAATTGCTAGAATTTTGAACCATTCCATTTTTCATATTATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATc                                         <  2:1475563/150‑1 (MQ=255)
                                                                                                          tCTTTAGCTAATATCAGTTGTGAATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCt                                        <  1:412126/150‑1 (MQ=255)
                                                                                                           cTTTAGCTTATATCAGTTGTGGATTGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACTCACAAAGAGATCTg                                       <  1:1281844/150‑1 (MQ=255)
                                                                                                           cTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTg                                       >  2:388092/1‑150 (MQ=255)
                                                                                                           cTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTg                                       <  1:917250/150‑1 (MQ=255)
                                                                                                              tAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAAc                                    <  2:923323/150‑1 (MQ=255)
                                                                                                               aGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACa                                   >  1:248120/1‑150 (MQ=255)
                                                                                                                   aaTCTCATTTGTTGATTTTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTCTAACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAATAGATCTGAACATCtt                               <  1:325841/150‑1 (MQ=255)
                                                                                                                   aaTATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCtt                               <  1:1001355/150‑1 (MQ=255)
                                                                                                                     tatCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaa                             <  2:1120884/150‑1 (MQ=255)
                                                                                                                       tCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAAt                           >  2:105067/1‑150 (MQ=255)
                                                                                                                        aaGTTTTGGATTGTTCAAAATTGCTAAAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATa                          <  1:1496644/149‑1 (MQ=255)
                                                                                                                         aGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATaa                         >  1:769737/1‑150 (MQ=255)
                                                                                                                          gTTGTGGATGGTTCAAAATTGCTACAAATTATAACCATTCCATTTTTCATAATATTAATTTGTTATTATTACACCCTATAACTTCTCTCGTCATATACTAGTATTATCAGTTAGTTAAACACAAAAAGAGATCTGAAAATAATaaaaaaa                        >  2:1640119/1‑149 (MQ=255)
                                                                                                                            tgtgGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGATATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGAc                      <  1:17406/150‑1 (MQ=255)
                                                                                                                              tgGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTa                    >  2:825261/1‑150 (MQ=255)
                                                                                                                               ggATGGTTCAAAATTGCTTCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAACCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTAAGTTAAACACTCAAAGAGATCTGAACATCTTAAATATGACTAt                   >  1:103305/1‑150 (MQ=255)
                                                                                                                               ggATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTAt                   >  2:889871/1‑150 (MQ=255)
                                                                                                                                  tGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTa                <  2:439788/150‑1 (MQ=255)
                                                                                                                                  tGGTTAAAAATTGCTACAATTTTGAAACATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTa                <  1:88953/150‑1 (MQ=255)
                                                                                                                                    gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg              >  1:95018/1‑150 (MQ=255)
                                                                                                                                    gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg              >  1:251954/1‑150 (MQ=255)
                                                                                                                                    gTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg              >  2:1306257/1‑150 (MQ=255)
                                                                                                                                       cAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGAATATTTAAGATg           >  2:983857/1‑150 (MQ=255)
                                                                                                                                           aTTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATa       <  1:422968/150‑1 (MQ=255)
                                                                                                                                           aTTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATa       <  1:388092/150‑1 (MQ=255)
                                                                                                                                              gCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTAATATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGATgttcagat    <  1:1670435/150‑8 (MQ=255)
                                                                                                                                               cTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACtt   >  1:942575/1‑150 (MQ=255)
                                                                                                                                                tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTa  <  1:1445181/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
ATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTA  >  NC_000913/2986960‑2987253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: