Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,140,352 G→C 5.8% R150G (CGC→GGC) ‡ hybE ← hydrogenase 2‑specific chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,3520GC 5.8% 454.1 / 15.3 138R150G (CGC→GGC) ‡hybEhydrogenase 2‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base G (65/65);  new base C (5/3);  total (70/68)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

Only 100 of 141 total aligned reads displayed.

ACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCG  >  NC_000913/3140203‑3140500
                                                                                                                                                     |                                                                                                                                                    
aCGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                                                                                                      <  1:1813915/150‑1 (MQ=255)
 cGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGa                                                                                                                                                     >  1:1355487/1‑150 (MQ=255)
  gCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGAc                                                                                                                                                    <  1:1715270/150‑1 (MQ=255)
   cAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAACCAGCAGGGCGCGACg                                                                                                                                                   >  2:256206/1‑150 (MQ=255)
     ggAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                                                                                                                 >  2:635354/1‑150 (MQ=255)
     agagaGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                                                                                                                 >  1:1202967/2‑150 (MQ=255)
            gcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctgt                                                                                                                                          <  2:927834/150‑4 (MQ=255)
             cgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctgtt                                                                                                                                         <  2:1453464/150‑5 (MQ=255)
                cAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGt                                                                                                                                      <  1:1566529/150‑1 (MQ=255)
                 aaTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTACAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTa                                                                                                                                     <  1:755743/150‑1 (MQ=255)
                   tttCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACa                                                                                                                                   >  1:673929/1‑150 (MQ=255)
                   tttCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACa                                                                                                                                   >  1:440396/1‑150 (MQ=255)
                   tttCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCGGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACa                                                                                                                                   <  1:1770578/150‑1 (MQ=255)
                   tttCCAGCCACACGGCGGTGACGCGCGTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGAATTTTCGCCACTCCTGCGACCAAACCGCAGGGCGCGACGCCCTGCGTGTGGTACa                                                                                                                                   >  1:764692/1‑150 (MQ=255)
                     tCCAGCCACACGGCGGTGAGGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAGCGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATc                                                                                                                                 >  1:1797087/1‑150 (MQ=255)
                     tCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCAGTTTCGCCACTCCTGCGGACAAACAGCAGGGCGCGACTCCCTGCGTGTGGTaagtc                                                                                                                                 >  1:293462/1‑146 (MQ=255)
                       cAGCCCCACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCg                                                                                                                               <  1:305019/150‑1 (MQ=255)
                       cAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCg                                                                                                                               >  1:1206398/1‑150 (MQ=255)
                        aGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCACCGGCGCTCTGTCAAAGAGACACCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCgg                                                                                                                              <  2:761781/150‑1 (MQ=255)
                          ccACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACAGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAt                                                                                                                            <  2:379247/150‑1 (MQ=255)
                            acacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTc                                                                                                                          >  2:1661882/1‑150 (MQ=255)
                             cacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCg                                                                                                                         <  2:1513324/150‑1 (MQ=255)
                                    gTGACGCGCTTAACCTCGGGCTGCGCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTATCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTgg                                                                                                                  <  2:1655575/150‑1 (MQ=255)
                                          cgcTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGa                                                                                                            >  2:1072468/1‑150 (MQ=255)
                                          cgcTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGa                                                                                                            >  2:143044/1‑150 (MQ=255)
                                                aCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTCCATCCGGATTCGTGACTGGCAGCGAAAGGAt                                                                                                      >  2:103687/1‑150 (MQ=255)
                                                aCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAt                                                                                                      <  2:978525/150‑1 (MQ=255)
                                                aCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAt                                                                                                      <  1:1445616/150‑1 (MQ=255)
                                                 cATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATc                                                                                                     <  2:1821210/150‑1 (MQ=255)
                                                     gTGCTGCTCCGCCTGCCGTTGGATAATTTTAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTc                                                                                                 >  1:45104/1‑150 (MQ=255)
                                                      tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCg                                                                                                >  1:1017164/1‑150 (MQ=255)
                                                        ctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTg                                                                                              <  2:491375/150‑1 (MQ=255)
                                                        ctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTg                                                                                              <  1:489700/150‑1 (MQ=255)
                                                        ctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGAGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTg                                                                                              >  1:575404/1‑150 (MQ=255)
                                                         tgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGCCTGGCAGCGAAAGGATCATTCGTgc                                                                                             >  1:990335/1‑150 (MQ=255)
                                                          gctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGCCCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                            >  1:1798146/1‑150 (MQ=255)
                                                          gctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                            >  1:726085/1‑150 (MQ=255)
                                                          gatCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCAGGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGTCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                            <  2:1154307/148‑1 (MQ=255)
                                                           ctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCTTGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcgc                                                                                           <  1:672093/150‑1 (MQ=255)
                                                           ctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcgc                                                                                           <  2:1202967/150‑1 (MQ=255)
                                                           ctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcgc                                                                                           <  2:1202965/150‑1 (MQ=255)
                                                            tCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCa                                                                                          >  1:519657/1‑150 (MQ=255)
                                                            tCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCa                                                                                          >  2:118607/1‑150 (MQ=255)
                                                            tCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGCAGCGACGCCCTGCGTGTGGTACATCCTGATTCGTCACTGGCAGCTAAAGGATCATTCGTGCGCa                                                                                          >  2:292694/1‑150 (MQ=255)
                                                             ccGCCTGCCGTTGGATAATTTCAACGGCGCGCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAg                                                                                         <  1:1343049/150‑1 (MQ=255)
                                                                          gATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATcc                                                                                   <  1:1341199/143‑1 (MQ=255)
                                                                              aTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTg                                                                        >  1:1192484/1‑150 (MQ=255)
                                                                               tttCATCGGCGCTCTGGCAAAGAGTCAACTCTAGCTGTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTgg                                                                       <  1:1584669/150‑1 (MQ=255)
                                                                               tttCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTgg                                                                       <  2:927658/150‑1 (MQ=255)
                                                                               tttCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTgg                                                                       >  1:1434366/1‑150 (MQ=255)
                                                                                ttCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                      <  1:360756/150‑1 (MQ=255)
                                                                                 tCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACACCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGcc                                                                     <  1:239913/150‑1 (MQ=255)
                                                                                     cGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCctct                                                                 <  2:1577836/150‑1 (MQ=255)
                                                                                       gcgcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                               <  2:1540496/150‑1 (MQ=255)
                                                                                          ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAAt                                                            >  2:1037535/1‑150 (MQ=255)
                                                                                           tctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATc                                                           <  2:1811082/150‑1 (MQ=255)
                                                                                            ctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCg                                                          <  2:1206398/150‑1 (MQ=255)
                                                                                            ctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCg                                                          <  2:1362717/150‑1 (MQ=255)
                                                                                            ctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCg                                                          <  1:27268/150‑1 (MQ=255)
                                                                                            ctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGa                                                         <  2:268324/150‑1 (MQ=255)
                                                                                                cAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACAt                                                      >  1:772583/1‑150 (MQ=255)
                                                                                                     agaCAACTCATGCATTTTCGCCACTCCTGCGCCCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCCGGCGTTGGCCCTCTTCAATCGACATGCtgt                                                 >  2:104558/1‑150 (MQ=255)
                                                                                                     agaCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGCCTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCtgt                                                 <  2:1064070/150‑1 (MQ=255)
                                                                                                     agaCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGACAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCGCTCTTCAATCGACATGCtgt                                                 >  2:314705/1‑150 (MQ=255)
                                                                                                       aCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTTCGTGTGGTACATCCGGATTCGTGCCTGGCAGCGAAAGGATCAATCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTGCAATCGACATGCTGTGc                                               >  1:879155/1‑150 (MQ=255)
                                                                                                       aCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGc                                               <  2:588312/150‑1 (MQ=255)
                                                                                                       aCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGc                                               <  1:1088683/150‑1 (MQ=255)
                                                                                                        cAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                              <  2:1017164/150‑1 (MQ=255)
                                                                                                        cAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                              >  1:1239839/1‑150 (MQ=255)
                                                                                                        cAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                              >  1:557709/1‑150 (MQ=255)
                                                                                                         aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGa                                             >  2:717600/1‑150 (MQ=255)
                                                                                                         aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGa                                             >  2:1338840/1‑150 (MQ=255)
                                                                                                             cATGCATTTTCGCGACTCCTGCGACCACACAGCAGGGCGCGCCGCCCTGCGTGTGGTACATCCGGATTCGGGACTGGCAGCGAAAGGATCATTCGTGCGCAGACATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgc                                         <  1:79046/150‑1 (MQ=255)
                                                                                                                gCATTTTCGCCACTCCTGCTACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc                                      <  2:1355487/150‑1 (MQ=255)
                                                                                                                gCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc                                      <  2:526653/150‑1 (MQ=255)
                                                                                                                gCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc                                      <  2:519657/150‑1 (MQ=255)
                                                                                                                gCATATTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc                                      <  1:905923/150‑1 (MQ=255)
                                                                                                                 cATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggcg                                     <  1:118607/150‑1 (MQ=255)
                                                                                                                   ttttCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGGGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGAc                                   <  2:1338174/150‑1 (MQ=255)
                                                                                                                     ttCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACAt                                 >  1:1762716/1‑150 (MQ=255)
                                                                                                                       cGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCa                               >  1:568374/1‑150 (MQ=255)
                                                                                                                              ccTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGCTCATTCGTGCGCAGTCATCCGTAAGGCGTTGGCAGTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACa                        >  2:773467/1‑150 (MQ=255)
                                                                                                                               cTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGAGAAAGCGGCGACATCAGCGAACAg                       >  2:950689/1‑150 (MQ=255)
                                                                                                                                tGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAgg                      <  1:1600663/150‑1 (MQ=255)
                                                                                                                                 gCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACCTGCTGTGCGAAAGCGGCGACATCAGCGAACTggg                     >  1:1714027/1‑149 (MQ=255)
                                                                                                                                  cGACCAAACAGCAGTGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAGCATTCGTGCGCAGTCATCCGTCAGGGGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgag                    <  2:134251/150‑1 (MQ=255)
                                                                                                                                  cGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCATGCGTAGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgag                    >  2:484759/1‑150 (MQ=255)
                                                                                                                                  cGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgag                    <  1:818013/150‑1 (MQ=255)
                                                                                                                                     ccAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACCTCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCACTCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgagaga                 >  1:1293710/1‑150 (MQ=255)
                                                                                                                                     ccAAACAGCAGGGCGAGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgagaga                 <  1:240629/150‑1 (MQ=255)
                                                                                                                                      cAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGat                >  2:1571608/1‑150 (MQ=255)
                                                                                                                                        acacgcagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGatat              >  2:1550055/5‑150 (MQ=255)
                                                                                                                                        acacgcagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGACAACATCAATCGACATGCTGTGCGAAAGCTGCGACATCAGCGAACAGGAGAGatat              >  2:164716/5‑150 (MQ=255)
                                                                                                                                         cacgcagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATAtt             >  1:1105640/4‑150 (MQ=255)
                                                                                                                                         cacgcagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATAtt             >  1:445978/4‑150 (MQ=255)
                                                                                                                                           agcagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAATGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGc           <  1:724138/150‑1 (MQ=255)
                                                                                                                                           agcagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGc           <  1:343061/150‑1 (MQ=255)
                                                                                                                                             cagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGa         >  1:1348645/1‑150 (MQ=255)
                                                                                                                                             cagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGa         <  2:684561/150‑1 (MQ=255)
                                                                                                                                              agGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACCTCAGCGAACAGGAGAGAGATTGCgca        >  1:89384/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCG  >  NC_000913/3140203‑3140500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: