Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,171,857 A→T 5.3% intergenic (+24/+22) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8570AT 5.3% 363.7 / 5.5 127intergenic (+24/+22)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (64/56);  new base T (5/2);  total (69/58)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 129 total aligned reads displayed.

CAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAA  >  NC_000913/3171711‑3172006
                                                                                                                                                  |                                                                                                                                                     
cAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                                                                    >  2:1697723/1‑150 (MQ=255)
  caCCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACg                                                                                                                                                  <  2:245851/149‑1 (MQ=255)
   aCCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAAACGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGa                                                                                                                                                 >  1:258409/1‑150 (MQ=255)
     cGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTTAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAct                                                                                                                                               <  2:933434/150‑1 (MQ=255)
        tACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActcta                                                                                                                                            >  2:1383269/1‑149 (MQ=255)
         aCCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCa                                                                                                                                           >  1:1457315/1‑150 (MQ=255)
          ccGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCAt                                                                                                                                          >  2:1586100/1‑150 (MQ=255)
           cGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATa                                                                                                                                         <  1:224028/150‑1 (MQ=255)
            ggCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAg                                                                                                                                        <  1:695002/150‑1 (MQ=255)
            ggCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAg                                                                                                                                        >  2:868485/1‑150 (MQ=255)
              cAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAt                                                                                                                                      >  1:1475488/1‑150 (MQ=255)
              cAGCGGGCTTGGGCTAGCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAGGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAt                                                                                                                                      <  1:1068769/150‑1 (MQ=255)
               aGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGGCGTTTTAACGGCTCTCATAGaca                                                                                                                                     <  2:858848/150‑3 (MQ=255)
                 ggggCTTGGGCTATCGATTGTCCAGCGCATCGCCATCTTGCATGGCATGAATGGTGAATTTGGGAATGCGCAACAAGGTGGATTTGAGGCGACGGTAAGCTGGTAACTTCTCTGTCTAGGAGAGCCGTTAAAACGACTCTCATAGAtttt                                                                                                                                   <  1:456004/149‑1 (MQ=255)
                 cGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGTATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAtttt                                                                                                                                   <  1:749044/150‑1 (MQ=255)
                   ggCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                                                                                 <  2:1688878/150‑1 (MQ=255)
                    gtttGGGCTTTCGTTTGTGCAGCTAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGATTGCGGAACAAGGGGGATTTGAGGCTAAGGTATGCTGGTAACTTCTCTGTCTATGAGTGCCGTTAAAACGACTCTCATAGATTTTACt                                                                                                                                <  2:1363716/148‑1 (MQ=255)
                    gattGGGCTATCGATTTTCCAGCGAATCGCCAAATTGCATGGCATGAATTTTTAATTTGGGAATGCGGAACAAGGTGTATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                                                                                <  1:1406764/148‑1 (MQ=255)
                       tGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                                                                             >  1:1826262/1‑150 (MQ=255)
                          gCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAACCGACTCTCATAGACTTTACTAATAGc                                                                                                                          >  1:1186102/1‑150 (MQ=255)
                          gCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                          >  1:1564224/1‑150 (MQ=255)
                          gCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGAGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCAGTCTATTAGAGCCGTTAAAACGACTCTCCTAGATTTTACGAATAGc                                                                                                                          >  2:294597/1‑150 (MQ=255)
                               cGATTGTCCAGCGAAACGCCAAATTGCATGGCATGAATGTTGAAATTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat                                                                                                                     >  1:1273843/1‑150 (MQ=255)
                                gATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                                                                                    <  2:1057693/150‑1 (MQ=255)
                                 aTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAatat                                                                                                                   >  2:1034117/1‑150 (MQ=255)
                                    gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAAAAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                                                                                <  1:573928/150‑1 (MQ=255)
                                     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                                                                               >  2:1007268/1‑150 (MQ=255)
                                     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                                                                               >  1:1549579/1‑150 (MQ=255)
                                       cAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCg                                                                                                             >  2:752787/1‑150 (MQ=255)
                                       cAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAAGACTCTCATAGATTTTACTAATAGCAAAATATAAACCg                                                                                                             >  2:773410/1‑150 (MQ=255)
                                          cGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcc                                                                                                          >  1:1486105/1‑150 (MQ=255)
                                             aTCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACAGTCCCCa                                                                                                       >  2:154493/1‑150 (MQ=255)
                                                   aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                                                                 <  2:498644/150‑1 (MQ=255)
                                                      ttGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                                                                              <  1:1347293/150‑1 (MQ=255)
                                                      ttGAATGTCATGAATTTTTAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCTAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                                                                              <  1:917755/150‑1 (MQ=255)
                                                           tggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                                                                         <  2:1207924/150‑1 (MQ=255)
                                                              aattaaTGTTTAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                      <  2:810523/146‑1 (MQ=255)
                                                               atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcaca                                                                                     <  2:1335886/150‑1 (MQ=255)
                                                                tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaa                                                                                    <  2:1486105/150‑1 (MQ=255)
                                                                tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTATCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaa                                                                                    >  1:1813613/1‑150 (MQ=255)
                                                                    tGTTGAATTTGTGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTTTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCa                                                                                <  2:225991/150‑1 (MQ=255)
                                                                    tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCa                                                                                >  2:1499467/1‑150 (MQ=255)
                                                                     gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGCCTATGAGAGCCGTTAAAACTACTCTCATAGATTTTACTAATAGCAAAATATAAACCTTCCCCAAAAAAGCCACCAACCACAAACCAg                                                                               >  2:1335430/1‑150 (MQ=255)
                                                                      ttGAATTTGGGAATGCGGAACTAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAACGCCACCAACCACAAACCAGa                                                                              >  2:877146/1‑150 (MQ=255)
                                                                      ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                                                                              >  1:949568/1‑150 (MQ=255)
                                                                         aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                                                                           >  1:141261/1‑150 (MQ=255)
                                                                              gggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATATTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATaac                                                                      >  2:70844/1‑148 (MQ=255)
                                                                               ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAccc                                                                     >  1:552496/1‑149 (MQ=255)
                                                                                gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                                                                    >  2:671650/1‑150 (MQ=255)
                                                                                  aTGCTGAACAAGGTTGATTTGAGTCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                  <  1:1043946/150‑1 (MQ=255)
                                                                                  aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                  <  1:119091/150‑1 (MQ=255)
                                                                                   tGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                                 >  2:1771958/1‑150 (MQ=255)
                                                                                     cGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                               >  1:152249/1‑150 (MQ=255)
                                                                                     cGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                               >  2:781012/1‑150 (MQ=255)
                                                                                         aCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACAAACCACAAACCAGACAAATACCGAGCGAGAATa                                                           >  2:1161737/1‑150 (MQ=255)
                                                                                          cAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATTGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAc                                                          <  2:1046876/150‑1 (MQ=255)
                                                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACa                                                         <  2:680200/150‑1 (MQ=255)
                                                                                             ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTGTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                       <  2:797676/150‑1 (MQ=255)
                                                                                             ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                       >  2:526876/1‑150 (MQ=255)
                                                                                                 gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAcc                                                   <  1:868485/150‑1 (MQ=255)
                                                                                                    ttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCaa                                                >  1:105218/1‑150 (MQ=255)
                                                                                                         ccGAAGGTAAGCTTGAAAATTCTCTGTCTATTATCTCCTATAAAACTACTCTCATAGATTTTACTAAAAGAAAAATATAAACCGTCCCCAAAAAAGCCAACAACCACAAACCAGACAAAAACCGAGCGAGAATACATATACCCAAAACTc                                           <  1:362756/149‑1 (MQ=255)
                                                                                                           gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGa                                         >  1:505421/1‑150 (MQ=255)
                                                                                                            aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGaa                                        <  1:1471515/150‑1 (MQ=255)
                                                                                                             aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                       <  2:949568/150‑1 (MQ=255)
                                                                                                              ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTTAAACGCCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTACCCAAAAAAGCCACCAGCCACAAACCAGACAAATACCTAGCGAGAATCCATATACCCAAAACTCGAACa                                      >  1:55131/1‑150 (MQ=255)
                                                                                                                 aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt                                   >  1:802121/1‑150 (MQ=255)
                                                                                                                 aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACAAATACCCAAAACTCGAACAttt                                   >  1:27024/1‑150 (MQ=255)
                                                                                                                  aGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTc                                  <  2:547552/150‑1 (MQ=255)
                                                                                                                     tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCg                               <  1:162703/150‑1 (MQ=255)
                                                                                                                      ggTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGc                              >  1:66035/1‑150 (MQ=255)
                                                                                                                        tAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCAt                            <  2:869306/150‑1 (MQ=255)
                                                                                                                          aCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaa                          >  1:1779962/1‑150 (MQ=255)
                                                                                                                          aCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaa                          <  1:752787/150‑1 (MQ=255)
                                                                                                                             tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga                       <  2:1662126/150‑1 (MQ=255)
                                                                                                                             tctcTGACTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAAAAACAAACCACACAAATACCGATCGAGAATACATATACCCACACCTCGCACAGTACCCGCATAAaga                       >  1:75635/1‑150 (MQ=255)
                                                                                                                              atctGTCTATGAGAGACGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag                      <  1:255240/149‑1 (MQ=255)
                                                                                                                                 tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGttt                   <  1:1046228/150‑1 (MQ=255)
                                                                                                                                 tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCCAGAAAACATAAACCCAAAACACGAACATATCCCGCATAAAGAGatt                   >  2:434949/1‑150 (MQ=255)
                                                                                                                                  gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt                  >  1:1189423/1‑150 (MQ=255)
                                                                                                                                  gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt                  >  1:603753/1‑150 (MQ=255)
                                                                                                                                   tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                 >  2:987581/1‑150 (MQ=255)
                                                                                                                                   tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                 <  2:1826262/150‑1 (MQ=255)
                                                                                                                                   gcTATGAGAGCAGTTAAAAAGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCAGCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTGCCGCATAAAGAGTTTTc                 <  2:159864/149‑1 (MQ=255)
                                                                                                                                    cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAAAAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCACACAAAAACCGAGCGAGAATACAAATACCCAAAAAAAGAACATATCCCGACTAAAGGGATTTCt                >  2:1377087/1‑150 (MQ=255)
                                                                                                                                    cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTACCCAACAAAGCCACAAAACACAAACAACACAAATACCGAGCGAGAATACATATACCCAAAACTAGAACATTTCCCTCATAAATAGTTATca                >  2:264486/1‑149 (MQ=255)
                                                                                                                                     tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCCGACAAATACCGAGCGAGAATACATATACCCAAAACGCGACCATTTCCCGCATAAAGAGTTTTCtt               >  1:897398/1‑150 (MQ=255)
                                                                                                                                      aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTta              >  1:1593979/1‑150 (MQ=255)
                                                                                                                                      aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTta              >  2:648909/1‑150 (MQ=255)
                                                                                                                                      aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTta              <  2:46378/150‑1 (MQ=255)
                                                                                                                                       tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa             >  1:342989/1‑150 (MQ=255)
                                                                                                                                         agagCCGTTAAAACGACTCTCATAGATTTTACTAATATCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACCAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaaga           <  1:916424/150‑1 (MQ=255)
                                                                                                                                          gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat          >  1:355539/1‑150 (MQ=255)
                                                                                                                                            gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCCCCAACCCCAACCCAGACAAATACCTAGCGAGAATACATATACCCAAACCTCGACCATTTCCCGCATAAAGATTTTTCTtaagataa        >  2:1306731/1‑150 (MQ=255)
                                                                                                                                            gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa        >  2:1372223/1‑150 (MQ=255)
                                                                                                                                            gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa        >  1:605824/1‑150 (MQ=255)
                                                                                                                                            gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa        >  2:1519994/1‑150 (MQ=255)
                                                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataag       <  2:86129/150‑1 (MQ=255)
                                                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaa     >  1:1535018/1‑150 (MQ=255)
                                                                                                                                                  aaaaCGACTCTCATAGAATTTACTAATAGCAAAATATAAACCGTCAAAAAAAAAGACACAAACCACAAACCAGACAAATACAGACCGAGAATAAATAAACACAAAACTCGAACATTTCACACATAAAGAGTTTTCTAAAGATAAGaataa  >  1:773459/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAA  >  NC_000913/3171711‑3172006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: