Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,153 A→T 5.7% K497* (AAA→TAA) ‡ fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,1530AT 5.7% 392.4 / 4.9 131K497* (AAA→TAA) ‡fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (56/65);  new base T (3/6);  total (59/72)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

ACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCC  >  NC_000913/3233004‑3233301
                                                                                                                                                     |                                                                                                                                                    
acacCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGcaaca                                                                                                                                                      <  1:545832/150‑1 (MQ=255)
 cacCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGAGCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGcaacaa                                                                                                                                                     >  1:940211/1‑150 (MQ=255)
  acCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACaaa                                                                                                                                                    <  1:1156333/150‑1 (MQ=255)
   ccGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCTTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCTCCGGTTGGCAACAAAg                                                                                                                                                   >  2:461821/1‑150 (MQ=255)
        aCGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGTGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCTATGTACTGCGCGAGAAAGCGCCGGTTGGCAACAAAGTTGcc                                                                                                                                              <  2:608132/150‑1 (MQ=255)
         cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                             <  2:1564143/150‑1 (MQ=255)
            cggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaacc                                                                                                                                          <  2:731080/150‑4 (MQ=255)
            cggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCcatca                                                                                                                                          >  1:1467188/1‑150 (MQ=255)
              gATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGATGGCAACTTTGTTGCcaaccgg                                                                                                                                        <  1:889619/150‑6 (MQ=255)
                 cAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGt                                                                                                                                     <  2:1027726/150‑1 (MQ=255)
                  aGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCGGCGCACTCCGCCCATCGACGGGATCGATCATCCTAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGtt                                                                                                                                    <  1:1795968/150‑1 (MQ=255)
                   gTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTCCTGCGCGCCAAAGCGCCGGTTGGCAACAACGTTGCCATCCTCGGTtg                                                                                                                                   >  1:320861/1‑150 (MQ=255)
                   gTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTtg                                                                                                                                   >  1:763970/1‑150 (MQ=255)
                          gCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCTCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCggg                                                                                                                            <  2:1458910/150‑1 (MQ=255)
                          gCTTGCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGGGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCggg                                                                                                                            <  1:186212/150‑1 (MQ=255)
                             ttCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGAtt                                                                                                                         <  1:1269158/150‑1 (MQ=255)
                                 aTGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtt                                                                                                                     <  1:179558/150‑1 (MQ=255)
                                 aTGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtt                                                                                                                     >  1:1259696/1‑150 (MQ=255)
                                  tGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGCCAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGGTGTGGCGGGATTGGttt                                                                                                                    >  1:861063/1‑150 (MQ=255)
                                   gAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACTAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtttt                                                                                                                   >  1:128961/1‑150 (MQ=255)
                                   gAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtttt                                                                                                                   >  2:1424977/1‑150 (MQ=255)
                                    aaaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGACATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                                  >  1:1003805/1‑150 (MQ=255)
                                    aaaCGATCCTCGCCAGT‑GGATCGTGCCGCGCACTCCGCCCCTCGACGGGCTCGATCATCCGAAGGGATTGAGTTATATCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCAAAATCGGTTGTGGCGGGTTTGGttttta                                                                                                                 >  2:460426/1‑148 (MQ=255)
                                    aaaCGATCCTCGACAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                                  <  2:1721259/150‑1 (MQ=255)
                                       cGATCCTCGCCAGTGGGATCGTGTCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAACGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATa                                                                                                               >  1:1027678/1‑150 (MQ=255)
                                           gcTCGCCTGTGGGTTCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTTAGTTATCTCGATGTACTGCGCGAGAAAGCGGCGGTTGGCTACTAAGTTGCCTTCATCGGTTGTGGCGGGATTGGTTTTGATACGGc                                                                                                           <  2:454110/149‑1 (MQ=255)
                                             tCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGa                                                                                                         <  1:703844/150‑1 (MQ=255)
                                              cGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGAt                                                                                                        >  2:493007/1‑150 (MQ=255)
                                               gCCAGTGGGATCGTGCCGCGCCCTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATg                                                                                                       >  1:515546/1‑150 (MQ=255)
                                                ccAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGt                                                                                                      <  2:14102/150‑1 (MQ=255)
                                                   gTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCCTCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAtt                                                                                                   >  2:1468026/1‑150 (MQ=255)
                                                   gTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAtt                                                                                                   <  2:1570818/150‑1 (MQ=255)
                                                     gggATCGTGCCGCGCACTCCGCCCATCGACGGTATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACTAAGCGCCGGTTGGCAACAAAGGTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTa                                                                                                 <  2:1342531/150‑1 (MQ=255)
                                                      ggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTaa                                                                                                >  1:1762595/1‑150 (MQ=255)
                                                      ggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGGTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTaa                                                                                                >  1:1166648/1‑150 (MQ=255)
                                                       gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                               <  2:739525/150‑1 (MQ=255)
                                                       gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                               <  1:643513/150‑1 (MQ=255)
                                                       gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                               >  1:1495999/1‑150 (MQ=255)
                                                        aTCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGt                                                                                              >  1:1031573/1‑150 (MQ=255)
                                                         tCGTGCCGCGCACTCCGCACATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGTCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGTTTTTGATACGGCGATGTATTTAAGTc                                                                                             <  2:564540/150‑1 (MQ=255)
                                                            tGCCGCTCACTCCGCCCATCGACGGGATCGAGCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGc                                                                                          <  1:779859/150‑1 (MQ=255)
                                                              ccGCGCACTCCGCCCATCGACGGGTTCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCg                                                                                        <  1:99205/150‑1 (MQ=255)
                                                              ccGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCg                                                                                        >  2:1105858/1‑150 (MQ=255)
                                                                   cACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGa                                                                                   >  1:1414091/1‑150 (MQ=255)
                                                                    aCTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGaa                                                                                  <  1:540102/150‑1 (MQ=255)
                                                                      tCCGCCCCTCGACGGGTTCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACACAGCGCCTGTTGGCAACAAATTTTCCATCATCGTTTGCGGCGGGATTGGATTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATc                                                                                <  1:1561129/150‑1 (MQ=255)
                                                                       ccGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATcc                                                                               >  1:1697574/1‑150 (MQ=255)
                                                                            cATCGACGGGATCGAGCAGCCGCAGGTTTTGAGTCATCTCGATGTACTCCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccag                                                                          <  2:157246/150‑1 (MQ=255)
                                                                                gACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccagccag                                                                      <  2:232805/150‑1 (MQ=255)
                                                                                  cGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGaa                                                                    <  1:477637/150‑1 (MQ=255)
                                                                                    ggATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAata                                                                  >  1:549696/1‑150 (MQ=255)
                                                                                      atcgatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATc                                                                >  2:1166738/1‑150 (MQ=255)
                                                                                       tcgatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCg                                                               <  2:313894/150‑1 (MQ=255)
                                                                                         gatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGcc                                                             >  1:681068/1‑150 (MQ=255)
                                                                                         gatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGcc                                                             >  2:1081646/1‑150 (MQ=255)
                                                                                           tcatcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCgg                                                           <  1:434424/150‑1 (MQ=255)
                                                                                            catcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTGTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCggg                                                          <  2:1342959/150‑1 (MQ=255)
                                                                                               ccGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTc                                                       >  2:1401235/1‑150 (MQ=255)
                                                                                                 gAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGATGCCATCATCGGTTGTGGCGGGATTGGGTTTGATACGGCGATGTATTTAAGGCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTg                                                     <  1:1319829/150‑1 (MQ=255)
                                                                                                 gAAGGTATTGAGTAATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAACTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTg                                                     >  2:486770/1‑150 (MQ=255)
                                                                                                    ggTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTAAGCCTGGCGAATCCACCAGCCAGAAAATCGCCGGGTTCTGaaa                                                  >  1:321161/1‑150 (MQ=255)
                                                                                                     gTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaat                                                 <  2:542885/150‑1 (MQ=255)
                                                                                                     gTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGCGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaat                                                 <  1:189777/150‑1 (MQ=255)
                                                                                                      tATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatg                                                >  1:1573430/1‑150 (MQ=255)
                                                                                                      tATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatg                                                <  2:619978/150‑1 (MQ=255)
                                                                                                       aTTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatga                                               >  2:132602/1‑150 (MQ=255)
                                                                                                       aTTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAACTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatga                                               >  1:727023/1‑150 (MQ=255)
                                                                                                         tGAGTTATCTCGATGTACTGCGCGACAATGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatgaat                                             <  2:355177/150‑1 (MQ=255)
                                                                                                         tGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatgaat                                             >  2:1393701/1‑150 (MQ=255)
                                                                                                         tGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGGTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatgaat                                             <  2:1683538/150‑1 (MQ=255)
                                                                                                            gTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggg                                          >  2:775836/1‑150 (MQ=255)
                                                                                                            gTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggg                                          <  2:1259696/150‑1 (MQ=255)
                                                                                                            gTTATCTCGATGTACTGCGCGACAAAGCGACGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCTGGATTGGTTTTGATACGCCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggg                                          >  1:453255/1‑150 (MQ=255)
                                                                                                              tATCTCGATGTTCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggggg                                        <  1:22436/150‑1 (MQ=255)
                                                                                                               aTCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATCCGGCGATGTATATAAGTCCGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGa                                       >  2:898401/1‑150 (MQ=255)
                                                                                                                  tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                    <  1:1081646/150‑1 (MQ=255)
                                                                                                                  tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGGTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                    <  2:752450/150‑1 (MQ=255)
                                                                                                                   cGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGa                                   >  2:666943/1‑150 (MQ=255)
                                                                                                                         aCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAg                             <  2:326737/150‑1 (MQ=255)
                                                                                                                         aCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAg                             >  1:1553571/1‑150 (MQ=255)
                                                                                                                           tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                           <  2:1818133/150‑1 (MQ=255)
                                                                                                                           tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                           <  2:1746317/150‑1 (MQ=255)
                                                                                                                           tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                           <  1:1726010/150‑1 (MQ=255)
                                                                                                                            gcgcGACAAAGCGCCGGTTGGCAACCAAGTTGCCAGCATCGGGTGGGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTGCTGTAATGAATGGGGGATCGACAGTAGCCt                          <  2:260708/150‑1 (MQ=255)
                                                                                                                            gcgcGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCt                          <  2:1467188/150‑1 (MQ=255)
                                                                                                                            gcgcGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCt                          >  1:1522712/1‑150 (MQ=255)
                                                                                                                             cgcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGAGAGTAGCCTa                         >  1:347059/1‑150 (MQ=255)
                                                                                                                                 aCAAAGCGCCGGATGGCAACACAGTTGCCATCATCGGTGGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGAGCGACAGTAGCCTacaac                     <  2:1090809/150‑1 (MQ=255)
                                                                                                                                   aaaGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAg                   >  2:1421467/1‑150 (MQ=255)
                                                                                                                                     aGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGAGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAAAAGGc                 >  1:933999/1‑150 (MQ=255)
                                                                                                                                        gcCGGTTTGCAACTAAGTTTCGATCTTCGGTTGTGGCTGGATTGGTTTTGATAGGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCTGGTTCTGTAATGAATTGGGGATCGACAGTAGCCTACATCAGGCtgg              <  2:842876/150‑1 (MQ=255)
                                                                                                                                        gcCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtgg              <  2:844926/150‑1 (MQ=255)
                                                                                                                                         ccGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggt             <  1:942149/150‑1 (MQ=255)
                                                                                                                                          cGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg            <  1:362748/150‑1 (MQ=255)
                                                                                                                                          cGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACAAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg            <  2:1673608/150‑1 (MQ=255)
                                                                                                                                          atgatGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg            >  1:457135/5‑150 (MQ=255)
                                                                                                                                            gTTGGCAACAAAGTTGCCATGATCGGGTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGc          <  1:392339/150‑1 (MQ=255)
                                                                                                                                              tGGCAACAAAGTTGCCATCATCGGTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCtt        <  2:940211/150‑1 (MQ=255)
                                                                                                                                                 caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCTGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAg     <  2:248866/150‑1 (MQ=255)
                                                                                                                                                  aacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGc    <  2:1003805/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCC  >  NC_000913/3233004‑3233301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑