Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,154 A→T 5.1% K497I (AAA→ATA) ‡ fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,1540AT 5.1% 390.1 / 7.0 131K497I (AAA→ATA) ‡fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (56/67);  new base T (3/4);  total (59/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 136 total aligned reads displayed.

CACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCC  >  NC_000913/3233005‑3233301
                                                                                                                                                     |                                                                                                                                                   
cacCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGAGCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGcaacaa                                                                                                                                                     >  1:940211/1‑150 (MQ=255)
 acCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACaaa                                                                                                                                                    <  1:1156333/150‑1 (MQ=255)
  ccGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCTTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCTCCGGTTGGCAACAAAg                                                                                                                                                   >  2:461821/1‑150 (MQ=255)
    gTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGtt                                                                                                                                                 >  2:189777/1‑150 (MQ=255)
        cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                             <  1:1739200/150‑1 (MQ=255)
        cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                             <  2:1564143/150‑1 (MQ=255)
           cggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaacc                                                                                                                                          <  2:731080/150‑4 (MQ=255)
              aTCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaaccggc                                                                                                                                       <  1:42811/150‑7 (MQ=255)
              aTCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCg                                                                                                                                       >  1:542885/1‑150 (MQ=255)
                cAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGt                                                                                                                                     <  2:1027726/150‑1 (MQ=255)
                 aGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCGGCGCACTCCGCCCATCGACGGGATCGATCATCCTAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGtt                                                                                                                                    <  1:1795968/150‑1 (MQ=255)
                  gTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTCCTGCGCGCCAAAGCGCCGGTTGGCAACAACGTTGCCATCCTCGGTtg                                                                                                                                   >  1:320861/1‑150 (MQ=255)
                         gCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCTCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCggg                                                                                                                            <  2:1458910/150‑1 (MQ=255)
                            ttCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGAtt                                                                                                                         <  1:1269158/150‑1 (MQ=255)
                                aTGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtt                                                                                                                     >  1:1259696/1‑150 (MQ=255)
                                aTGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtt                                                                                                                     <  1:179558/150‑1 (MQ=255)
                                 tGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGCCAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGGTGTGGCGGGATTGGttt                                                                                                                    >  1:861063/1‑150 (MQ=255)
                                  gAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACTAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtttt                                                                                                                   >  1:128961/1‑150 (MQ=255)
                                  gAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtttt                                                                                                                   <  2:1426926/150‑1 (MQ=255)
                                   aaaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGACATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                                  >  1:1003805/1‑150 (MQ=255)
                                   aaaCGATCCTCGCCAGT‑GGATCGTGCCGCGCACTCCGCCCCTCGACGGGCTCGATCATCCGAAGGGATTGAGTTATATCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCAAAATCGGTTGTGGCGGGTTTGGttttta                                                                                                                 >  2:460426/1‑148 (MQ=255)
                                   aaaCGATCCTCGACAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                                  <  2:1721259/150‑1 (MQ=255)
                                      cGATCCTCGCCAGTGGGATCGTGTCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAACGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATa                                                                                                               >  1:1027678/1‑150 (MQ=255)
                                          gcTCGCCTGTGGGTTCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTTAGTTATCTCGATGTACTGCGCGAGAAAGCGGCGGTTGGCTACTAAGTTGCCTTCATCGGTTGTGGCGGGATTGGTTTTGATACGGc                                                                                                           <  2:454110/149‑1 (MQ=255)
                                           cTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCg                                                                                                          >  2:1065267/1‑150 (MQ=255)
                                            tCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGa                                                                                                         <  1:703844/150‑1 (MQ=255)
                                             cGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGAt                                                                                                        >  2:493007/1‑150 (MQ=255)
                                              gCCAGTGGGATCGTGCCGCGCCCTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATg                                                                                                       >  1:515546/1‑150 (MQ=255)
                                                  gTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAtt                                                                                                   <  2:1570818/150‑1 (MQ=255)
                                                    gggATCGTGCCGCGCACTCCGCCCATCGACGGTATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACTAAGCGCCGGTTGGCAACAAAGGTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTa                                                                                                 <  2:1342531/150‑1 (MQ=255)
                                                    gggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTa                                                                                                 >  1:1818133/1‑150 (MQ=255)
                                                    gggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTa                                                                                                 <  2:313698/150‑1 (MQ=255)
                                                     ggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGGTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTaa                                                                                                >  1:1166648/1‑150 (MQ=255)
                                                      gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                               <  2:739525/150‑1 (MQ=255)
                                                      gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                               <  1:643513/150‑1 (MQ=255)
                                                      gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                               >  1:1495999/1‑150 (MQ=255)
                                                       aTCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGt                                                                                              >  1:1031573/1‑150 (MQ=255)
                                                        tCGTGCCGCGCACTCCGCACATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGTCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGTTTTTGATACGGCGATGTATTTAAGTc                                                                                             <  2:564540/150‑1 (MQ=255)
                                                           tGCCGCTCACTCCGCCCATCGACGGGATCGAGCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGc                                                                                          <  1:779859/150‑1 (MQ=255)
                                                             ccGCGCACTCCGCCCATCGACGGGTTCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCg                                                                                        <  1:99205/150‑1 (MQ=255)
                                                             ccGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCg                                                                                        >  2:1105858/1‑150 (MQ=255)
                                                                 gcACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCg                                                                                    <  1:821430/150‑1 (MQ=255)
                                                                  cACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGa                                                                                   >  1:1414091/1‑150 (MQ=255)
                                                                   aCTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGaa                                                                                  <  1:540102/150‑1 (MQ=255)
                                                                     tCCGCCCCTCGACGGGTTCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACACAGCGCCTGTTGGCAACAAATTTTCCATCATCGTTTGCGGCGGGATTGGATTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATc                                                                                <  1:1561129/150‑1 (MQ=255)
                                                                      ccGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATcc                                                                               >  1:1697574/1‑150 (MQ=255)
                                                                        gCCCATCGACGGGATCGATCATCCGAACGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATccac                                                                             >  1:958152/1‑150 (MQ=255)
                                                                               gACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccagccag                                                                      <  2:232805/150‑1 (MQ=255)
                                                                               gACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccagccag                                                                      <  2:1141964/150‑1 (MQ=255)
                                                                                   ggATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAata                                                                  >  2:1572471/1‑150 (MQ=255)
                                                                                     atcgatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATc                                                                >  2:1166738/1‑150 (MQ=255)
                                                                                      tcgatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCg                                                               <  2:313894/150‑1 (MQ=255)
                                                                                       cgatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGATTATCGc                                                              >  1:921361/1‑150 (MQ=255)
                                                                                        gatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGcc                                                             >  2:1081646/1‑150 (MQ=255)
                                                                                          tcatcCGAAGGTATTGAGCTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGG‑TTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCggg                                                          <  2:61342/150‑1 (MQ=255)
                                                                                              ccGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTc                                                       >  2:1401235/1‑150 (MQ=255)
                                                                                              ccGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGATGGCAACAAAGTTGCCATCATCGGTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTc                                                       <  1:71989/150‑1 (MQ=255)
                                                                                                gAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGATGCCATCATCGGTTGTGGCGGGATTGGGTTTGATACGGCGATGTATTTAAGGCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTg                                                     <  1:1319829/150‑1 (MQ=255)
                                                                                                gAAGGTATTGAGTAATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAACTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTg                                                     >  2:486770/1‑150 (MQ=255)
                                                                                                   ggTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTAAGCCTGGCGAATCCACCAGCCAGAAAATCGCCGGGTTCTGaaa                                                  >  1:321161/1‑150 (MQ=255)
                                                                                                    gTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaat                                                 <  2:542885/150‑1 (MQ=255)
                                                                                                    gTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGCGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaat                                                 <  1:189777/150‑1 (MQ=255)
                                                                                                     tATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatg                                                >  1:1573430/1‑150 (MQ=255)
                                                                                                     tATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatg                                                <  2:619978/150‑1 (MQ=255)
                                                                                                      aTTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatga                                               >  2:132602/1‑150 (MQ=255)
                                                                                                      aTTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAACTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatga                                               >  1:727023/1‑150 (MQ=255)
                                                                                                        tGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatgaat                                             >  2:1393701/1‑150 (MQ=255)
                                                                                                        tGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGGTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatgaat                                             <  2:1683538/150‑1 (MQ=255)
                                                                                                           gTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggg                                          >  2:775836/1‑150 (MQ=255)
                                                                                                           gTTATCTCGATGTACTGCGCGACAAAGCGACGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCTGGATTGGTTTTGATACGCCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggg                                          >  1:453255/1‑150 (MQ=255)
                                                                                                            ttATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATgggg                                         <  2:1414091/150‑1 (MQ=255)
                                                                                                            ttATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATgggg                                         <  2:1304192/150‑1 (MQ=255)
                                                                                                             tATCTCGATGTTCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggggg                                        <  1:22436/150‑1 (MQ=255)
                                                                                                              aTCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATCCGGCGATGTATATAAGTCCGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGa                                       >  2:898401/1‑150 (MQ=255)
                                                                                                              aTCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGa                                       <  2:1538009/150‑1 (MQ=255)
                                                                                                                 tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                    <  1:1081646/150‑1 (MQ=255)
                                                                                                                 tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGGTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                    <  2:752450/150‑1 (MQ=255)
                                                                                                                  cGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGa                                   >  2:666943/1‑150 (MQ=255)
                                                                                                                       tACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTa                              >  1:557941/1‑150 (MQ=255)
                                                                                                                        aCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAg                             <  2:326737/150‑1 (MQ=255)
                                                                                                                        aCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAg                             >  1:1553571/1‑150 (MQ=255)
                                                                                                                          tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                           <  2:1746317/150‑1 (MQ=255)
                                                                                                                          tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                           <  1:1726010/150‑1 (MQ=255)
                                                                                                                           gcgcGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCt                          <  2:1467188/150‑1 (MQ=255)
                                                                                                                           gcgcGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCt                          >  1:1522712/1‑150 (MQ=255)
                                                                                                                            cgcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGAGAGTAGCCTa                         >  1:347059/1‑150 (MQ=255)
                                                                                                                              cgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTaca                       <  1:1465116/150‑1 (MQ=255)
                                                                                                                                  aaaGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAg                   >  2:1421467/1‑150 (MQ=255)
                                                                                                                                    aGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGAGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAAAAGGc                 >  1:933999/1‑150 (MQ=255)
                                                                                                                                       gcCGGTTTGCAACTAAGTTTCGATCTTCGGTTGTGGCTGGATTGGTTTTGATAGGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCTGGTTCTGTAATGAATTGGGGATCGACAGTAGCCTACATCAGGCtgg              <  2:842876/150‑1 (MQ=255)
                                                                                                                                       gcCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtgg              <  2:844926/150‑1 (MQ=255)
                                                                                                                                        ccGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggt             >  2:13650/1‑150 (MQ=255)
                                                                                                                                         cGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg            <  1:362748/150‑1 (MQ=255)
                                                                                                                                         cGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACAAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg            <  2:1673608/150‑1 (MQ=255)
                                                                                                                                         atgatGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg            >  1:457135/5‑150 (MQ=255)
                                                                                                                                         atgatGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGAAATGAATGGGGGATCGACAGTAACCTACAACAGGCtggtg            >  2:392538/5‑150 (MQ=255)
                                                                                                                                           gTTGGCAACAAAGTTGCCATGATCGGGTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGc          <  1:392339/150‑1 (MQ=255)
                                                                                                                                            ttGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCt         <  1:460981/150‑1 (MQ=255)
                                                                                                                                             tGGCAACAAAGTTGCCATCATCGGTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCtt        <  2:940211/150‑1 (MQ=255)
                                                                                                                                                caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCTGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAg     <  2:248866/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCC  >  NC_000913/3233005‑3233301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑