Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,155 A→T 5.2% K497N (AAA→AAT) ‡ fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,1550AT 5.2% 402.3 / 8.5 134K497N (AAA→AAT) ‡fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (55/71);  new base T (3/4);  total (59/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 136 total aligned reads displayed.

ACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCA  >  NC_000913/3233006‑3233304
                                                                                                                                                     |                                                                                                                                                     
acCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACaaa                                                                                                                                                       <  1:1156333/150‑1 (MQ=255)
      aCGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGTGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCTATGTACTGCGCGAGAAAGCGCCGGTTGGCAACAAAGTTGcc                                                                                                                                                 <  2:608132/150‑1 (MQ=255)
      aCGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGcc                                                                                                                                                 <  2:1699136/150‑1 (MQ=255)
       cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                                <  1:1739200/150‑1 (MQ=255)
       cggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCca                                                                                                                                                <  2:1564143/150‑1 (MQ=255)
             aTCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACTTTGTTGCcaaccggc                                                                                                                                          <  1:42811/150‑7 (MQ=255)
             aTCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCg                                                                                                                                          >  1:542885/1‑150 (MQ=255)
                aGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCGGCGCACTCCGCCCATCGACGGGATCGATCATCCTAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGtt                                                                                                                                       <  1:1795968/150‑1 (MQ=255)
                 gTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTCCTGCGCGCCAAAGCGCCGGTTGGCAACAACGTTGCCATCCTCGGTtg                                                                                                                                      >  1:320861/1‑150 (MQ=255)
                        gCTTTCGATGAAACGATCCTGGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGAGCGATCATCCTAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCggg                                                                                                                               <  2:862950/150‑1 (MQ=255)
                        gCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCTCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCggg                                                                                                                               <  2:1458910/150‑1 (MQ=255)
                           ttCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGAtt                                                                                                                            <  1:1269158/150‑1 (MQ=255)
                             cGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTgg                                                                                                                          >  2:460981/1‑150 (MQ=255)
                               aTGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtt                                                                                                                        >  1:1259696/1‑150 (MQ=255)
                               aTGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtt                                                                                                                        <  1:179558/150‑1 (MQ=255)
                                tGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGCCAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGGTGTGGCGGGATTGGttt                                                                                                                       >  1:861063/1‑150 (MQ=255)
                                 gAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACTAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtttt                                                                                                                      >  1:128961/1‑150 (MQ=255)
                                 gAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGtttt                                                                                                                      >  2:1424977/1‑150 (MQ=255)
                                  aaaCGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGACATCATCGGTTGTGGCGGGATTGGTTTTg                                                                                                                     >  1:1003805/1‑150 (MQ=255)
                                  aaaCGATCCTCGCCAGT‑GGATCGTGCCGCGCACTCCGCCCCTCGACGGGCTCGATCATCCGAAGGGATTGAGTTATATCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCAAAATCGGTTGTGGCGGGTTTGGttttta                                                                                                                    >  2:460426/1‑148 (MQ=255)
                                     cGATCCTCGCCAGTGGGATCGTGTCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAACGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATa                                                                                                                  >  1:1027678/1‑150 (MQ=255)
                                         gcTCGCCTGTGGGTTCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTTAGTTATCTCGATGTACTGCGCGAGAAAGCGGCGGTTGGCTACTAAGTTGCCTTCATCGGTTGTGGCGGGATTGGTTTTGATACGGc                                                                                                              <  2:454110/149‑1 (MQ=255)
                                          cTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCg                                                                                                             >  2:1065267/1‑150 (MQ=255)
                                           tCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGa                                                                                                            <  1:703844/150‑1 (MQ=255)
                                            cGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGAt                                                                                                           >  2:493007/1‑150 (MQ=255)
                                             gCCAGTGGGATCGTGCCGCGCCCTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATg                                                                                                          >  1:515546/1‑150 (MQ=255)
                                              ccAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGt                                                                                                         <  2:14102/150‑1 (MQ=255)
                                                 gTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCCTCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAtt                                                                                                      >  2:1468026/1‑150 (MQ=255)
                                                 gTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTAtt                                                                                                      <  2:1570818/150‑1 (MQ=255)
                                                   gggATCGTGCCGCGCACTCCGCCCATCGACGGTATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACTAAGCGCCGGTTGGCAACAAAGGTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTa                                                                                                    <  2:1342531/150‑1 (MQ=255)
                                                   gggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTa                                                                                                    >  1:1818133/1‑150 (MQ=255)
                                                    ggATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGGTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTaa                                                                                                   >  1:1166648/1‑150 (MQ=255)
                                                     gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                                  <  2:739525/150‑1 (MQ=255)
                                                     gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                                  <  1:643513/150‑1 (MQ=255)
                                                     gATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAg                                                                                                  >  1:1495999/1‑150 (MQ=255)
                                                      aTCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGt                                                                                                 >  1:1031573/1‑150 (MQ=255)
                                                       tCGTGCCGCGCACTCCGCACATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGTCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGTTTTTGATACGGCGATGTATTTAAGTc                                                                                                <  2:564540/150‑1 (MQ=255)
                                                          tGCCGCTCACTCCGCCCATCGACGGGATCGAGCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGc                                                                                             <  1:779859/150‑1 (MQ=255)
                                                            ccGCGCACTCCGCCCATCGACGGGTTCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCg                                                                                           <  1:99205/150‑1 (MQ=255)
                                                                gcACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCg                                                                                       <  1:821430/150‑1 (MQ=255)
                                                                 cACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGa                                                                                      >  1:1414091/1‑150 (MQ=255)
                                                                  aCTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGaa                                                                                     <  1:540102/150‑1 (MQ=255)
                                                                    tCCGCCCCTCGACGGGTTCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACACAGCGCCTGTTGGCAACAAATTTTCCATCATCGTTTGCGGCGGGATTGGATTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATc                                                                                   <  1:1561129/150‑1 (MQ=255)
                                                                     ccGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATcc                                                                                  >  1:1697574/1‑150 (MQ=255)
                                                                          cATCGACGGGATCGAGCAGCCGCAGGTTTTGAGTCATCTCGATGTACTCCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccag                                                                             <  2:157246/150‑1 (MQ=255)
                                                                              gACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccagccag                                                                         <  2:1141964/150‑1 (MQ=255)
                                                                              gACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCAccagccag                                                                         <  2:232805/150‑1 (MQ=255)
                                                                                    atcgatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATc                                                                   >  2:1166738/1‑150 (MQ=255)
                                                                                     tcgatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCg                                                                  <  2:313894/150‑1 (MQ=255)
                                                                                      cgatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGATTATCGc                                                                 >  1:921361/1‑150 (MQ=255)
                                                                                       gatcATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGcc                                                                >  2:1081646/1‑150 (MQ=255)
                                                                                          catcCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTGTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCggg                                                             <  2:1342959/150‑1 (MQ=255)
                                                                                             ccGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTc                                                          >  2:1401235/1‑150 (MQ=255)
                                                                                             ccGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGATGGCAACAAAGTTGCCATCATCGGTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTc                                                          <  1:71989/150‑1 (MQ=255)
                                                                                               gAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGATGCCATCATCGGTTGTGGCGGGATTGGGTTTGATACGGCGATGTATTTAAGGCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTg                                                        <  1:1319829/150‑1 (MQ=255)
                                                                                               gAAGGTATTGAGTAATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAACTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTg                                                        >  2:486770/1‑150 (MQ=255)
                                                                                                  ggTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTAAGCCTGGCGAATCCACCAGCCAGAAAATCGCCGGGTTCTGaaa                                                     >  1:321161/1‑150 (MQ=255)
                                                                                                   gTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGCGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaat                                                    <  1:189777/150‑1 (MQ=255)
                                                                                                    tATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatg                                                   >  1:1573430/1‑150 (MQ=255)
                                                                                                    tATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatg                                                   <  2:619978/150‑1 (MQ=255)
                                                                                                     aTTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatga                                                  >  2:132602/1‑150 (MQ=255)
                                                                                                     aTTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAACTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatga                                                  >  1:727023/1‑150 (MQ=255)
                                                                                                       tGAGTTATCTCGATGTACTGCGCGACAATGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatgaat                                                <  2:355177/150‑1 (MQ=255)
                                                                                                       tGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatgaat                                                >  2:1393701/1‑150 (MQ=255)
                                                                                                       tGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGGTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTaatgaat                                                <  2:1683538/150‑1 (MQ=255)
                                                                                                          gTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggg                                             <  2:1259696/150‑1 (MQ=255)
                                                                                                          gTTATCTCGATGTACTGCGCGACAAAGCGACGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCTGGATTGGTTTTGATACGCCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggg                                             >  1:453255/1‑150 (MQ=255)
                                                                                                            tATCTCGATGTTCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATggggg                                           <  1:22436/150‑1 (MQ=255)
                                                                                                             aTCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATCCGGCGATGTATATAAGTCCGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGa                                          >  2:898401/1‑150 (MQ=255)
                                                                                                                tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                       <  1:1081646/150‑1 (MQ=255)
                                                                                                                tcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGGTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCg                                       <  2:752450/150‑1 (MQ=255)
                                                                                                                 cGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGa                                      >  2:666943/1‑150 (MQ=255)
                                                                                                                      tACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTa                                 >  1:557941/1‑150 (MQ=255)
                                                                                                                       aCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAg                                >  1:1553571/1‑150 (MQ=255)
                                                                                                                       aCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAg                                <  2:326737/150‑1 (MQ=255)
                                                                                                                         tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                              <  1:1726010/150‑1 (MQ=255)
                                                                                                                         tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                              <  2:1746317/150‑1 (MQ=255)
                                                                                                                         tGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGcc                              <  2:1818133/150‑1 (MQ=255)
                                                                                                                          gcgcGACAAAGCGCCGGTTGGCAACCAAGTTGCCAGCATCGGGTGGGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTGCTGTAATGAATGGGGGATCGACAGTAGCCt                             <  2:260708/150‑1 (MQ=255)
                                                                                                                          gcgcGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCt                             >  1:1522712/1‑150 (MQ=255)
                                                                                                                          gcgcGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCt                             <  2:1467188/150‑1 (MQ=255)
                                                                                                                           cgcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGAGAGTAGCCTa                            >  1:347059/1‑150 (MQ=255)
                                                                                                                            gcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTac                           <  2:515546/150‑1 (MQ=255)
                                                                                                                             cgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTaca                          <  1:1465116/150‑1 (MQ=255)
                                                                                                                               aCAAAGCGCCGGATGGCAACACAGTTGCCATCATCGGTGGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGAGCGACAGTAGCCTacaac                        <  2:1090809/150‑1 (MQ=255)
                                                                                                                                 aaaGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAg                      >  2:1421467/1‑150 (MQ=255)
                                                                                                                                   aGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGAGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAAAAGGc                    >  1:933999/1‑150 (MQ=255)
                                                                                                                                      gcCGGTTTGCAACTAAGTTTCGATCTTCGGTTGTGGCTGGATTGGTTTTGATAGGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCTGGTTCTGTAATGAATTGGGGATCGACAGTAGCCTACATCAGGCtgg                 <  2:842876/150‑1 (MQ=255)
                                                                                                                                      gcCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtgg                 <  2:844926/150‑1 (MQ=255)
                                                                                                                                       ccGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggt                <  1:942149/150‑1 (MQ=255)
                                                                                                                                        cGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg               <  1:362748/150‑1 (MQ=255)
                                                                                                                                        cGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACAAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg               <  2:1673608/150‑1 (MQ=255)
                                                                                                                                        atgatGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTCGCCGAGAACAGGCtggtg               >  2:761695/5‑150 (MQ=255)
                                                                                                                                        atgatGGCAACTTTGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCtggtg               >  1:457135/5‑150 (MQ=255)
                                                                                                                                          gTTGGCAACAAAGTTGCCATGATCGGGTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGc             <  1:392339/150‑1 (MQ=255)
                                                                                                                                           ttGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCt            <  1:460981/150‑1 (MQ=255)
                                                                                                                                            tGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCtt           <  2:681068/150‑1 (MQ=255)
                                                                                                                                            tGGCAACAAAGTTGCCATCATCGGTTGAGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCtt           <  2:940211/150‑1 (MQ=255)
                                                                                                                                               caacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCTGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAg        <  2:248866/150‑1 (MQ=255)
                                                                                                                                                aacaaAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGc       <  2:1003805/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGATACGGCGATGTATTTAAGTCAGCCGGGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCA  >  NC_000913/3233006‑3233304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑