Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 357,778 C→T 6.6% intergenic (+324/+13) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,7780CT 6.6% 220.5 / 3.2 75intergenic (+324/+13)codA/cynRcytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (48/22);  new base T (2/3);  total (50/25)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

AACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG  >  NC_000913/357631‑357925
                                                                                                                                                   |                                                                                                                                                   
aaCGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTATTTTATCCCCAAAAACACGTGAAAGCAATAACCGGCCTACGTAGATAAATTAACTCGTATACCACATAAACGTAGGGAAGCAGGCACTTCCAGCCTCAGAcgg                                                                                                                                                   >  2:560707/1‑148 (MQ=12)
aaCGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCATGCAATTCCAGCCGCAGACCt                                                                                                                                                   <  2:1528258/150‑1 (MQ=255)
   gCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCtgtg                                                                                                                                                >  1:569565/1‑150 (MQ=255)
         tgCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCg                                                                                                                                          >  1:611905/1‑150 (MQ=255)
         ggCAAAAATGCCTTCGCAGCGGCCGTCTGCCAGCTGTAGTCTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATCAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCg                                                                                                                                          <  1:179903/149‑1 (MQ=255)
           cAAAAATGCATTCGCTGCGGCTGTCTGCCATCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGc                                                                                                                                        <  2:1481440/150‑1 (MQ=255)
              aaaTGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTAc                                                                                                                                     <  2:829446/150‑1 (MQ=255)
                 tGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCTTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGt                                                                                                                                  <  1:1326553/150‑1 (MQ=255)
                    cTTCGCTGCGTCTGTCTGCCTGGTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGAt                                                                                                                               <  2:1668031/150‑1 (MQ=255)
                    cTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGACTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGAGGCTACCGTGAt                                                                                                                               >  2:1724196/1‑150 (MQ=255)
                    cTTCGCAGCGGCTGTCTGCCAGCTATATTTAATCCCGGAAGAAGCCTGAAATCAATAACCTGCATACGTAGAGCAATGAAATCGTAGGCCTGATAAGAGTAGAGCATAAGGCAATTCCAGCCGCAGACCTCTGTCAGCGGCTAACGTGAt                                                                                                                               >  2:66427/1‑150 (MQ=255)
                       cGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCa                                                                                                                            >  1:1644642/1‑150 (MQ=255)
                        gCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTTCCGTGATTCAt                                                                                                                           >  1:637906/1‑150 (MQ=255)
                        gCAGCGGCTGTCTGCCAGCTGTAGTTAATGCCGGATGCGGCGTGAACGCCTTATCCGCCCTACGTAGAGCACTGAACTCGTAGGCCTGAATAGCGTAGCGCATCACGCAATTCCAGCCGCAGACCTGTGTCAGCGGCAACCGTGATTCAt                                                                                                                           >  2:1639145/1‑150 (MQ=17)
                         cAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCAtt                                                                                                                          >  2:508103/1‑150 (MQ=255)
                           gCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTc                                                                                                                        >  2:1579420/1‑150 (MQ=255)
                             ggCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCg                                                                                                                      >  1:193999/1‑150 (MQ=255)
                               cTGTCTGCAATCTTTAGTATCTGCCGTATGCTGCGTGAACGCATTAGACTGCCTACTTAGCTCCCGGTACTCGGTGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGcc                                                                                                                    <  1:1095303/150‑1 (MQ=14)
                                   cTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCCGCTACCGGGATTCATTTCCGCcaaca                                                                                                                >  2:326212/1‑150 (MQ=255)
                                     gCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCaacaac                                                                                                              >  2:1825836/1‑150 (MQ=255)
                                     gCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCaacaac                                                                                                              >  1:1763151/1‑150 (MQ=255)
                                     gCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCaacaac                                                                                                              >  1:1769993/1‑150 (MQ=255)
                                         gCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACcgcg                                                                                                          >  1:981623/1‑150 (MQ=40)
                                            gTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCAt                                                                                                       >  1:1457228/1‑150 (MQ=38)
                                              aGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGACTACCGTGATTCATTTCCGCCAACAACCGCGCAttt                                                                                                     >  2:1423189/1‑150 (MQ=38)
                                                     gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAAc                                                                                              >  1:1028042/1‑150 (MQ=14)
                                                      ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACg                                                                                             >  2:1518267/1‑150 (MQ=255)
                                                           tGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAtg                                                                                        <  2:24421/150‑1 (MQ=255)
                                                             gggcgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAtgtg                                                                                      <  1:1452173/149‑1 (MQ=255)
                                                             cggcgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTGGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAtgtg                                                                                      >  2:894277/1‑150 (MQ=255)
                                                               gcgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCAATTCCGCCAACAACCGCGCATATATCCAACGCCATGTGCa                                                                                    >  2:752824/1‑150 (MQ=255)
                                                                cgTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCaa                                                                                   >  1:1283699/1‑150 (MQ=255)
                                                                  tGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCaaaa                                                                                 >  1:690574/1‑150 (MQ=255)
                                                                   gAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCaaaaa                                                                                >  1:1321990/1‑150 (MQ=255)
                                                                       gCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCACCGCCATGTGCAAAAATGcc                                                                            >  1:810568/1‑150 (MQ=255)
                                                                        ccgcaTCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCt                                                                           <  2:1028042/146‑1 (MQ=255)
                                                                         cTTATCCGGCCTGCGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCtt                                                                          >  1:531470/1‑150 (MQ=255)
                                                                              ccGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACACCCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCgcgg                                                                     >  2:873717/1‑150 (MQ=255)
                                                                                ggCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCgcggcg                                                                   >  2:1286179/1‑150 (MQ=255)
                                                                                  ccTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCggcggc                                                                 >  2:1011705/1‑150 (MQ=255)
                                                                                    tACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCt                                                                                                                                       >  1:1553501/1‑78 (MQ=39)
                                                                                    tACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCt                                                                                                                                       <  2:1553501/78‑1 (MQ=39)
                                                                                               cTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCCGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCGCACCACCGCGCATTTATCCCACGCCATGTGCAAATATGCCTTCGCGGCGGCTGTCGGCCAGCTa                                                    >  2:309551/1‑150 (MQ=14)
                                                                                               cTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTa                                                    >  1:176494/1‑150 (MQ=2)
                                                                                               cTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTa                                                    >  2:939692/1‑150 (MQ=255)
                                                                                                tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAt                                                   >  1:1041205/1‑150 (MQ=255)
                                                                                                 gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGcc                                                                                                                    >  1:84218/1‑84 (MQ=18)
                                                                                                 gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGcc                                                                                                                    <  2:84218/84‑1 (MQ=18)
                                                                                                 gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAtt                                                  >  1:631147/1‑150 (MQ=255)
                                                                                                       gTAGGCCTGATAAGCGTAGCGCATAAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccg                                            <  2:1666220/150‑1 (MQ=255)
                                                                                                            ccTGATAAGCGTTGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGca                                       <  1:894277/150‑1 (MQ=255)
                                                                                                                     cGTATCGCTTCAGGCAATTCCAGCCTCAGACGTGTTTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg                              <  1:1400114/150‑1 (MQ=18)
                                                                                                                      gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgc                             >  2:708506/1‑150 (MQ=255)
                                                                                                                           gcATCAGGCAATTCCAGCCGCAGACCTGTGTCATCGGCTACCGTTATTCATTTCCTCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc                        <  2:569565/150‑1 (MQ=255)
                                                                                                                            cATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCggcggc                                                                 <  2:106407/108‑1 (MQ=255)
                                                                                                                            cATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTa                                                    <  1:1249070/121‑1 (MQ=255)
                                                                                                                            cATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTACCGCCAACAACCGCGCATTAATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTa                                                    >  2:1249070/1‑121 (MQ=255)
                                                                                                                            cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTTCAGTGATTCATTTCCTCAAACAACCGCGCATTTATCCCACTCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGACGTtct                       >  2:1111377/1‑150 (MQ=18)
                                                                                                                            cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtct                       >  2:1395156/1‑150 (MQ=255)
                                                                                                                               cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAACCCGCCGTtctcgc                    >  1:1754477/1‑148 (MQ=255)
                                                                                                                               cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc                    <  1:508103/150‑1 (MQ=255)
                                                                                                                                aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATATATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc                   <  1:1230977/150‑1 (MQ=255)
                                                                                                                                 ggCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCa                  >  2:957939/1‑150 (MQ=255)
                                                                                                                                  gCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCag                 <  2:531470/150‑1 (MQ=255)
                                                                                                                                   cAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGc                                                                                     <  2:1672320/81‑1 (MQ=255)
                                                                                                                                   cAATTCCAGCCGCTGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt                <  1:721188/150‑1 (MQ=255)
                                                                                                                                   cAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt                >  1:994186/1‑150 (MQ=255)
                                                                                                                                      ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtagt             <  1:1724196/150‑1 (MQ=255)
                                                                                                                                       tCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCACCGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGAAACAAAACCGCCGTTATCTCCAGTAGTg            >  2:1597756/1‑150 (MQ=255)
                                                                                                                                        ccAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTgg           >  1:650932/1‑150 (MQ=255)
                                                                                                                                         cAGCCGCAGACCTGTGTCAGCGGCTCCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGc          >  2:1404948/1‑150 (MQ=255)
                                                                                                                                          aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACACCCGCGCATTTATCCAACGCCATGTGCATAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg         >  2:1669222/1‑150 (MQ=255)
                                                                                                                                           gCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg        >  1:561074/1‑150 (MQ=255)
                                                                                                                                             cGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTACGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTCGCgggg      >  2:1600825/1‑150 (MQ=255)
                                                                                                                                              gCAGACCTGTGTCAGCGGCTACCGTGATTCAAATCCGCCAACACCCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc     <  1:66427/150‑1 (MQ=255)
                                                                                                                                                 gACCTGTGTCAGCGGCTACCGTGATTCATTTCAGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAACACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag  >  1:1609441/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG  >  NC_000913/357631‑357925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: