Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,790 A→G 5.4% intergenic (‑46/+37) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,7900AG 5.4% 447.6 / 10.7 149intergenic (‑46/+37)ygjV/uxaAImp‑YgjV family inner membrane protein/altronate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (67/73);  new base G (4/4);  total (72/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

Only 100 of 150 total aligned reads displayed.

GAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTCCAGC  >  NC_000913/3241641‑3241938
                                                                                                                                                     |                                                                                                                                                    
gAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTa                                                                                                                                                      >  2:1337441/1‑150 (MQ=255)
 aaaGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTAt                                                                                                                                                     <  1:1681928/150‑1 (MQ=255)
  aaGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATg                                                                                                                                                    <  1:174544/150‑1 (MQ=255)
   aGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCTTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGa                                                                                                                                                   >  1:343277/1‑150 (MQ=255)
   aGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTCGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGa                                                                                                                                                   <  1:1686957/150‑1 (MQ=255)
    gctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGaa                                                                                                                                                  <  1:1543976/150‑1 (MQ=255)
        ctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGc                                                                                                                                              >  2:432729/1‑150 (MQ=255)
         tttttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCTATTACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGcc                                                                                                                                             <  2:324577/150‑1 (MQ=255)
           tttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCATTCAGAAAGGCTATGCCACCCACGCCAGGGGCATGCCATTACGCGGTAATGATAATTCGTTTTAAGTTATGGATAACAAAACGGCGATTCACATTTTGAAACGcgtt                                                                                                                                           >  1:525001/1‑147 (MQ=255)
            ttGAAGCGACGTTCGTCACGATTGAAAATTGTTGTGATACTAATCAGAATGGCGTTGACACCCACTCCCTGGGCCAGCCATTACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGTTTCATAATGTGAAGCGCCGtt                                                                                                                                          <  2:1297120/150‑1 (MQ=255)
             tGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGttt                                                                                                                                         >  1:1438157/1‑150 (MQ=255)
               aaGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGttttt                                                                                                                                       >  2:488911/1‑150 (MQ=255)
                 gCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                                     <  2:1396792/150‑1 (MQ=255)
                  cGACGTTCGTCACGATTCAAAAATGGTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGGCATGATAACTCCTTAGCAGGTATGGAAAAGCAAACGGCGTTTCATAATGTGAAGCGCCGtttgcttt                                                                                                                                    <  1:1291397/150‑6 (MQ=38)
                   gACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTttatt                                                                                                                                   <  1:1688763/150‑1 (MQ=255)
                       ttCGTCACGATTGAAAAATGTTGTTATACCAATCAGAATGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATTGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTatca                                                                                                                               <  2:383215/150‑1 (MQ=255)
                         cGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTATCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaacaac                                                                                                                             <  1:730997/150‑1 (MQ=255)
                         cGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaacaac                                                                                                                             <  1:795265/150‑1 (MQ=255)
                           tCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTc                                                                                                                           <  1:214234/150‑1 (MQ=255)
                              cGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCAtt                                                                                                                        >  1:1614608/1‑150 (MQ=255)
                              cGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCAttt                                                                                                                       >  1:979852/1‑150 (MQ=255)
                                aTTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCTTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTTACAACTCATTTc                                                                                                                      <  2:474147/150‑1 (MQ=255)
                                aTTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTc                                                                                                                      >  2:161175/1‑150 (MQ=255)
                                 ttGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTATTATTAACAACTCATTTCg                                                                                                                     >  2:436071/1‑150 (MQ=255)
                                        aTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTtat                                                                                                              <  2:1641494/150‑1 (MQ=255)
                                        aTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTtat                                                                                                              >  2:1082184/1‑150 (MQ=255)
                                            tgtgATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCg                                                                                                          <  1:84607/150‑1 (MQ=255)
                                             gtgATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGt                                                                                                         >  1:746011/1‑150 (MQ=255)
                                               gATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACACCTCATTTCGACTTTATAGCGTTa                                                                                                       >  2:1183491/1‑150 (MQ=255)
                                                 tACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcg                                                                                                     >  1:1121435/1‑150 (MQ=255)
                                                  aCCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgc                                                                                                    >  1:1207372/1‑150 (MQ=255)
                                                   ccAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgcc                                                                                                   >  2:508806/1‑150 (MQ=255)
                                                   ccAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgcc                                                                                                   >  1:762943/1‑150 (MQ=255)
                                                   ccAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgcc                                                                                                   >  2:1712342/1‑150 (MQ=255)
                                                   ccAATCAGAAAGGCGATGACACCCACGCCCTGGCCAGCCAATACGCGGTCATGACAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgccg                                                                                                  >  2:181807/1‑150 (MQ=255)
                                                    cAATCAGAAAGGCGATGACACCCACGCCCTGGGCAAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCCCATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGAGTTTATAGCGTTAcgccg                                                                                                  >  2:266933/1‑150 (MQ=255)
                                                     aaTCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgccgc                                                                                                 >  2:1082350/1‑150 (MQ=255)
                                                      aTCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCt                                                                                                >  2:557474/1‑150 (MQ=255)
                                                                 gATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCg                                                                                     >  2:1645289/1‑150 (MQ=255)
                                                                 gATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCg                                                                                     <  2:561818/150‑1 (MQ=255)
                                                                 gATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCg                                                                                     <  1:429345/150‑1 (MQ=255)
                                                                  aTGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                                    >  2:1310419/1‑150 (MQ=255)
                                                                   tGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGcc                                                                                   <  1:101329/150‑1 (MQ=255)
                                                                   tGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGcc                                                                                   <  1:968924/150‑1 (MQ=255)
                                                                     acacCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATTAAACGCCTTTTTTTATTAAAAACAAATTACCACTTAATATAGTAACGCTGATTTTGAAGATAGCAAg                                                                                 >  2:224290/1‑150 (MQ=255)
                                                                           aagCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAAAGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTcgcg                                                                           <  2:912625/148‑1 (MQ=255)
                                                                           aCGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATTGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATCTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTcgcg                                                                           <  2:796717/150‑1 (MQ=255)
                                                                           aCGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTcgcg                                                                           <  2:804054/150‑1 (MQ=255)
                                                                               ccTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAg                                                                       <  1:1192755/150‑1 (MQ=255)
                                                                               ccTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAg                                                                       <  1:1822052/150‑1 (MQ=255)
                                                                                  gggCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCg                                                                    <  1:986424/150‑1 (MQ=255)
                                                                                    gccagccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGtt                                                                  >  1:48503/1‑150 (MQ=255)
                                                                                     ccagccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTa                                                                 <  2:1759599/150‑1 (MQ=255)
                                                                                     ccagccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTa                                                                 <  1:1404717/150‑1 (MQ=255)
                                                                                       agccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCTGAAGTCGTTACg                                                               >  2:921921/1‑150 (MQ=255)
                                                                                        gccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGc                                                              <  1:432729/150‑1 (MQ=255)
                                                                                           aaTACGCCGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCg                                                           <  2:350886/150‑1 (MQ=255)
                                                                                            aTACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTAATTTCTCCTTTATAGCGTTACGCCGCTATTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGt                                                          >  1:1173899/1‑150 (MQ=255)
                                                                                               cgcgGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTAc                                                       >  1:1600821/1‑150 (MQ=255)
                                                                                                 cgttCATGATAAATCCTTAGCATGTATGGACAAGCTAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAg                                                     <  1:260220/147‑1 (MQ=255)
                                                                                                   gTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGt                                                   >  2:1761013/1‑150 (MQ=255)
                                                                                                     cATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGttt                                                 >  2:1291509/1‑150 (MQ=255)
                                                                                                        gATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCt                                              <  1:996058/150‑1 (MQ=255)
                                                                                                         aTAAATCCTTAGCTGGTATGGAAACGCAAACGGCGCTTCACTTTATGAACCGCCGTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTa                                            <  2:343277/150‑1 (MQ=255)
                                                                                                         aTAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCtt                                             <  1:693630/150‑1 (MQ=255)
                                                                                                         aTAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCtt                                             >  1:567890/1‑150 (MQ=255)
                                                                                                           aaaTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTAc                                           <  2:670380/150‑1 (MQ=255)
                                                                                                           aaaTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTAc                                           <  2:1488654/150‑1 (MQ=255)
                                                                                                            aaTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTAcc                                          >  1:381802/1‑150 (MQ=255)
                                                                                                             aTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGTCTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCg                                         <  2:1363710/150‑1 (MQ=255)
                                                                                                                cTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGGAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTg                                      >  1:59466/1‑150 (MQ=255)
                                                                                                                cTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTg                                      <  2:59925/150‑1 (MQ=255)
                                                                                                                cTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGATACGCCGCTTTTGAAGATCGCCAGTTCGCTGAAGTCGTTACGCTCGTTACAGGTTGGCTTACCGTTg                                      >  1:1149182/1‑150 (MQ=255)
                                                                                                                cTTAGCAGGTATGGAAAAGCAAACGGCGCGTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGTGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTg                                      >  1:135478/1‑150 (MQ=255)
                                                                                                                  tAGCAGGTATGGAAAATCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGc                                    <  2:242918/150‑1 (MQ=255)
                                                                                                                  tAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGc                                    <  1:1752666/150‑1 (MQ=255)
                                                                                                                  tAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATCAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGACACTTCGCGGAAGTCGTTACGCGCGTTACAGGTTTGCTTAACGTTTGc                                    >  2:153475/1‑150 (MQ=255)
                                                                                                                   aGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCa                                   <  1:473171/150‑1 (MQ=255)
                                                                                                                    gCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCaa                                  >  1:886084/1‑150 (MQ=255)
                                                                                                                      aGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAAc                                <  1:734781/150‑1 (MQ=255)
                                                                                                                        gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGTAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTc                              >  1:983966/1‑150 (MQ=255)
                                                                                                                          attgAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCaa                            <  1:637939/147‑1 (MQ=255)
                                                                                                                          aTGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCaa                            <  1:1031484/150‑1 (MQ=255)
                                                                                                                          aTGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATAGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCaa                            >  1:1658056/1‑150 (MQ=255)
                                                                                                                           tGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTGTATGAACACCTCATTTCGACTTTATAGCGTTACGCCGCTTTTTAAGATCGCCAGATCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGGTGGCAAACTCAAc                           >  2:203452/1‑150 (MQ=255)
                                                                                                                            ggAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACg                          >  1:160275/1‑150 (MQ=255)
                                                                                                                            ggAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACg                          >  2:1271350/1‑150 (MQ=255)
                                                                                                                             taataaaaaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGa                         >  2:1808443/8‑150 (MQ=38)
                                                                                                                               ataaaaaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATg                       >  2:446927/6‑150 (MQ=39)
                                                                                                                               aaaGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATg                       >  1:1421322/1‑150 (MQ=255)
                                                                                                                                      aCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGa                >  1:1183023/1‑150 (MQ=255)
                                                                                                                                       cGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGAt               >  1:1534972/1‑150 (MQ=255)
                                                                                                                                         gcgcTTCACATTATGAAACGCCGTTTTTTATTAACCACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCATCGATGGTGTCGATaa             >  1:1327003/1‑150 (MQ=255)
                                                                                                                                         gcgcTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACACGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATaa             >  2:1004076/1‑150 (MQ=255)
                                                                                                                                          cgcTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGAGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATaaa            >  1:238715/1‑150 (MQ=255)
                                                                                                                                               cacaTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttctt       >  2:858808/1‑150 (MQ=255)
                                                                                                                                                acaTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttcttc      <  1:161175/150‑1 (MQ=255)
                                                                                                                                                acaTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttcttc      >  1:755355/1‑150 (MQ=255)
                                                                                                                                                 caTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTcc     >  2:89885/1‑150 (MQ=255)
                                                                                                                                                 caTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTcc     <  1:508806/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTCCAGC  >  NC_000913/3241641‑3241938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: