Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,441 T→G 9.0% T71P (ACG→CCG)  yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4410TG 9.0% 253.8 / 14.2 107T71P (ACG→CCG) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (57/40);  new base G (4/6);  total (61/46)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

Only 100 of 107 total aligned reads displayed.

ATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTT  >  NC_000913/3256293‑3256588
                                                                                                                                                    |                                                                                                                                                   
aTCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTg                                                                                                                                                    >  2:576628/1‑150 (MQ=255)
 tCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGc                                                                                                                                                   >  1:397396/1‑150 (MQ=255)
  cTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGcc                                                                                                                                                  >  1:44446/1‑150 (MQ=255)
  cTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCAATCCCGTGcc                                                                                                                                                  >  2:762522/1‑150 (MQ=255)
  attAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGcc                                                                                                                                                  <  2:619278/149‑1 (MQ=255)
    taccGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCAGCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCTCAATCGGCAGCCCCACCATTCCCGGGCCgg                                                                                                                                                <  1:302687/147‑1 (MQ=255)
    tAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCgg                                                                                                                                                <  1:1696588/150‑1 (MQ=255)
       cGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGaa                                                                                                                                             <  2:810918/150‑1 (MQ=255)
          agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACgg                                                                                                                                          <  2:578747/150‑1 (MQ=255)
           gaCTTTCCCCGCCGCCAGCAGAGCTTATGAATCGGCAATTGACTGCGCTGTAGCGTCTAAAAGCACTTCCACCCCGACGAAGGCAATAACACATAACGCAACAAGCGCCGCCGCAAACAGCATACAAACAATTCCCATGCCGGGAAAGGt                                                                                                                                         >  2:440314/1‑150 (MQ=255)
             cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTgg                                                                                                                                       >  1:775503/1‑149 (MQ=255)
             cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTACCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgg                                                                                                                                       <  1:959888/150‑2 (MQ=255)
              tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGGCTTTCAGCACATCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGAc                                                                                                                                      <  1:1368226/150‑1 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgggg                                                                                                                                     <  2:1812861/150‑4 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                                                                                     <  1:149834/150‑1 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                                                                                     >  1:1641392/1‑150 (MQ=255)
                tCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTTTTGCGTCTTTCATCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggc                                                                                                                                    <  2:591712/150‑5 (MQ=255)
                tCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGc                                                                                                                                    <  2:1504164/150‑1 (MQ=255)
                  cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAGTCGGCAGCCCCACCATACCCGTGCCGGGAACGGTGACGccc                                                                                                                                  >  1:1496613/1‑150 (MQ=255)
                  cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                                  <  1:107658/150‑1 (MQ=255)
                  cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGAGACGccc                                                                                                                                  >  2:1735932/1‑150 (MQ=255)
                  cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGACTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCAGACGCAATCGTCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                                  >  1:229725/1‑150 (MQ=255)
                   ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCa                                                                                                                                 <  1:1296994/150‑1 (MQ=255)
                        gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGAcag                                                                                                                            >  2:1136809/1‑148 (MQ=255)
                            gcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttct                                                                                                                        <  1:1767584/150‑1 (MQ=255)
                              agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGttcttc                                                                                                                      >  2:1186815/1‑150 (MQ=255)
                                 gCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatc                                                                                                                   >  2:60237/1‑150 (MQ=255)
                                 gCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatc                                                                                                                   <  1:1623320/150‑1 (MQ=255)
                                 gCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCACCCAGCGCCGCCGCAATCGGCAGCCCCACCATTACCGTGACGGGAACGGTGACGCCCAGACCGTTCTtcata                                                                                                                   >  2:18075/1‑149 (MQ=255)
                                  cTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGGTCTtcatca                                                                                                                  >  1:1727254/1‑150 (MQ=255)
                                   tttGGCATCGGCAATTGCCTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAg                                                                                                                 <  1:1158168/150‑1 (MQ=255)
                                      ggCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAtt                                                                                                              >  1:1382985/1‑150 (MQ=255)
                                       gCATCGGCAATTGCCTGCGCCGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCAATCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAttt                                                                                                             <  2:323928/150‑1 (MQ=255)
                                            ggCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGa                                                                                                        >  2:1084612/1‑150 (MQ=255)
                                                      tGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACCGTGACGCCCAGACCGTTCTTCATCAGAATTGGCGAAACCCAGGCt                                                                                              >  1:1641603/1‑150 (MQ=255)
                                                         gcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCt                                                                                           <  1:1561319/150‑1 (MQ=255)
                                                           tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                         >  1:246360/1‑150 (MQ=255)
                                                           tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                         <  2:483189/150‑1 (MQ=255)
                                                              tGCGTCTTACAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                      <  1:785294/150‑1 (MQ=255)
                                                               gCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCGACAGATGTGGCGCAACCCAGGCTTCGACCCGt                                                                                     >  2:344010/1‑150 (MQ=255)
                                                                 gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                   >  1:440191/1‑150 (MQ=255)
                                                                     ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAcc                                                                               >  2:1328682/1‑150 (MQ=255)
                                                                       cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                             >  1:1294448/1‑150 (MQ=255)
                                                                         gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAc                                                                           >  1:348217/1‑150 (MQ=255)
                                                                          cACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAcc                                                                          >  2:443081/1‑150 (MQ=255)
                                                                           aCTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAccc                                                                         <  1:432924/150‑2 (MQ=255)
                                                                            cTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt                                                                        <  2:1593864/150‑1 (MQ=255)
                                                                              tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                      <  1:1735932/150‑1 (MQ=255)
                                                                              tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                      <  1:586363/150‑1 (MQ=255)
                                                                               ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCa                                                                     >  2:1578135/1‑150 (MQ=255)
                                                                                   cccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTc                                                                 <  1:436148/150‑1 (MQ=255)
                                                                                    ccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCCGCCCGTTCTTGAGCTGATTTGGCGAACCCCAGGCTTATACCCGGTCAACCGGACCTGCCAGGTCt                                                                >  1:918897/1‑150 (MQ=255)
                                                                                    ccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGGTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCCACCGGCCCTTCCAGTTCt                                                                >  1:1802187/1‑150 (MQ=255)
                                                                                      agCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGc                                                              >  1:1019167/2‑150 (MQ=255)
                                                                                       gCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGcc                                                             <  1:1084551/150‑1 (MQ=255)
                                                                                         gTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                           <  2:474440/150‑1 (MQ=255)
                                                                                         gTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                           <  2:44446/150‑1 (MQ=255)
                                                                                           tGGCATTTCCCCCTTAAGGCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcag                                                         <  1:754007/150‑1 (MQ=255)
                                                                                             gCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagca                                                       <  2:1337128/150‑1 (MQ=255)
                                                                                                tttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAct                                                    >  2:694784/1‑149 (MQ=255)
                                                                                                  tCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgc                                                  >  2:1564016/1‑150 (MQ=255)
                                                                                                    cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                >  2:1131296/1‑150 (MQ=255)
                                                                                                    cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                >  2:413736/1‑150 (MQ=255)
                                                                                                      ccTACCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAACGGTGCCGCCCAGGCCGTGCTACAGCAGATTTGGCGACACCCAGGCTTCGACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc                                              >  1:18078/1‑150 (MQ=255)
                                                                                                           cGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTCCACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCgccgccgcccg                                         >  1:607648/1‑148 (MQ=255)
                                                                                                           cGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccag                                         >  2:1357765/1‑150 (MQ=255)
                                                                                                             cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                       >  1:1389810/1‑150 (MQ=255)
                                                                                                                ccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGACCGGTGACGCCCAGCCCGTTCTTAATCAGATTTGGCGCAACCCAGGCTTCTACACGTTCAACCGGCCGTTCCAGTTCTGCCGCAGCAACCGCCGCcgcccgcgccc                                    >  2:1412477/1‑149 (MQ=255)
                                                                                                                   gcgcCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGTACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTATACACGTTCAACCGTACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGcccgtgcccgtg                                 >  2:47917/1‑150 (MQ=255)
                                                                                                                    cgccgccgcAATCGTCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGa                                <  2:795808/150‑1 (MQ=255)
                                                                                                                    cgccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGa                                <  1:747942/150‑1 (MQ=255)
                                                                                                                     gccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCAACCGCCGCCGCCAGCGCCAGTGaa                               >  2:1259264/1‑150 (MQ=255)
                                                                                                                      ccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGaaa                              <  1:443081/150‑1 (MQ=255)
                                                                                                                        gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                            >  1:1712210/1‑150 (MQ=255)
                                                                                                                         ccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg                           >  1:762689/1‑150 (MQ=255)
                                                                                                                          cgcAATGGGCAGCCGCACCTTTCCCGTGCCGGGACAGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGACCCCCAGGCTTCTACACGTTCCACCGGACCTTCCCGGTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                          <  1:290880/150‑1 (MQ=255)
                                                                                                                          cgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                          <  1:60237/150‑1 (MQ=255)
                                                                                                                          cgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                          >  2:12484/1‑150 (MQ=255)
                                                                                                                            cAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt                        >  2:229606/1‑150 (MQ=255)
                                                                                                                            cAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTACCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt                        <  1:1084612/150‑1 (MQ=255)
                                                                                                                             aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTc                       <  1:553017/150‑1 (MQ=255)
                                                                                                                              aTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCAGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCa                      <  2:515602/150‑1 (MQ=255)
                                                                                                                              aTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTGCATCAGATTAGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCACCCGCCGCCGCCAGCGCCCGTGCAAGCGGtgcc                      >  1:1165047/1‑147 (MQ=255)
                                                                                                                               tCGGCAGCCCCACCATTCCCGTGCCGGTAAGGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCTAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAg                     <  2:685167/150‑1 (MQ=255)
                                                                                                                               tCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACTGCCGCCGCCAGCGCCAGTGAAATCGGTTCAg                     <  1:694784/150‑1 (MQ=255)
                                                                                                                               tCGGCAGCCCAACAATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCTTATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAg                     <  2:341218/150‑1 (MQ=255)
                                                                                                                                cGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGt                    >  2:519513/1‑150 (MQ=255)
                                                                                                                                     gCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATc               >  2:426849/1‑150 (MQ=255)
                                                                                                                                      ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCCGGCTTCTTCACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc              >  1:972166/1‑150 (MQ=255)
                                                                                                                                      ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc              >  2:68644/1‑150 (MQ=255)
                                                                                                                                       cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCCTCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCCACCGCCGCCGCCAGCGCCAGTGAAATCGGATCAGTAAATccc             >  2:326168/1‑150 (MQ=255)
                                                                                                                                        tcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcccc            >  1:396902/2‑149 (MQ=255)
                                                                                                                                        tcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCa            >  2:164044/2‑150 (MQ=255)
                                                                                                                                        ccaccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcccc            >  1:1771747/1‑149 (MQ=255)
                                                                                                                                        ccaccaTTCCCGTGCCGCGACCGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGCCTTCTACACGTTCAACCCGACCTTCCAGTTCTGCCGCAGAAACCGCCGCCGCCAGCGCCAGTGAAATAGGTTCAGTACATCCCa            >  2:522913/1‑150 (MQ=255)
                                                                                                                                         caccgTTCCCGGCACGGGAATGGTGACGACCAAACCGTTCATCATCAGAATTGACTAAAACCAGGCTTATACAAGTTAAAAAGGCCATTCCATATATTAAGAAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg           >  2:198698/1‑150 (MQ=255)
                                                                                                                                                cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt    >  2:1037036/1‑150 (MQ=255)
                                                                                                                                                cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCAACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt    <  1:1221680/150‑1 (MQ=255)
                                                                                                                                                 ccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtt   >  1:796828/1‑150 (MQ=255)
                                                                                                                                                  ggTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGttt  <  1:1136809/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTT  >  NC_000913/3256293‑3256588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: