Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 361,901 T→G 5.6% intergenic (‑41/+25) lacA ← / ← lacY thiogalactoside acetyltransferase/lactose permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913361,9010TG 5.6% 378.6 / 7.9 141intergenic (‑41/+25)lacA/lacYthiogalactoside acetyltransferase/lactose permease
Reads supporting (aligned to +/- strand):  ref base T (70/62);  new base G (3/6);  total (73/68)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 142 total aligned reads displayed.

TAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGA  >  NC_000913/361752‑362047
                                                                                                                                                     |                                                                                                                                                  
tAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCt                                                                                                                                                    <  1:991163/150‑1 (MQ=255)
 aaaCTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTc                                                                                                                                                   >  1:689010/1‑150 (MQ=255)
    cTCATACGTTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTcgcg                                                                                                                                                >  1:1393221/1‑150 (MQ=255)
      cATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGcgcc                                                                                                                                              >  1:418714/1‑150 (MQ=255)
      cATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGcgcc                                                                                                                                              >  1:401677/1‑150 (MQ=255)
       aTACATTAACGTTTTCCCACGAATTCTTTTTTCCGGAAAGCGTTCGCACATATCGGTAAATAGCTTGCCTGCTGTTATAATTTCGGTCTTTGGCATGTGCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGAGCTCGcgccg                                                                                                                                             >  2:235946/1‑150 (MQ=255)
        tACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGcgccgc                                                                                                                                            >  2:1295512/1‑150 (MQ=255)
          cATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCAt                                                                                                                                          >  2:176972/1‑150 (MQ=255)
           aTTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATc                                                                                                                                         >  1:1613156/1‑150 (MQ=255)
           aTTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATc                                                                                                                                         >  1:1564994/1‑150 (MQ=255)
            ttAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATGCGGCGCGAGCGCCTTATcc                                                                                                                                        <  1:57475/150‑1 (MQ=255)
            ttAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATcc                                                                                                                                        >  2:445036/1‑150 (MQ=255)
            ttAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCAGTTATGATATGTTGGTCGGATAAGGCGCTCGAGCCGCATcc                                                                                                                                        >  1:531218/1‑150 (MQ=255)
               ccGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGAc                                                                                                                                     <  2:301903/149‑1 (MQ=255)
                cGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACa                                                                                                                                    >  2:1659718/1‑150 (MQ=255)
                 gTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATGCGGCGCGAGCGCCTTATCCGAcca                                                                                                                                   <  2:79709/150‑3 (MQ=255)
                  ttttCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACatt                                                                                                                                  <  2:401677/150‑1 (MQ=255)
                      cccACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgatt                                                                                                                              >  2:818299/1‑150 (MQ=255)
                      cccACGAAGTCTTTTTTACGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTACGTTATGATATGTTGGTCGGATAAGGCGCTAGCGCCGCATCCGACattgatt                                                                                                                              >  2:1392093/1‑150 (MQ=255)
                       ccACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgattg                                                                                                                             <  2:1536472/150‑1 (MQ=255)
                       ccACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgattg                                                                                                                             >  1:8174/1‑150 (MQ=255)
                       ccACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACagtgattg                                                                                                                             >  2:1672027/1‑150 (MQ=255)
                        cACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGc                                                                                                                            <  2:146075/150‑1 (MQ=255)
                        cACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGATTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGc                                                                                                                            >  2:1292817/1‑150 (MQ=255)
                             aGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAg                                                                                                                       >  2:105889/1‑150 (MQ=255)
                               tCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCg                                                                                                                     <  1:532106/150‑1 (MQ=255)
                               tCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCg                                                                                                                     >  1:1177088/1‑150 (MQ=255)
                                cTTTTTTCCGGTACGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGa                                                                                                                    >  2:178497/1‑150 (MQ=255)
                                cTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGa                                                                                                                    >  1:847326/1‑150 (MQ=255)
                                cTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGa                                                                                                                    >  1:1392729/1‑150 (MQ=255)
                                  tttttCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACt                                                                                                                  <  1:623880/150‑1 (MQ=255)
                                    tttCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttc                                                                                                                <  2:1408757/150‑1 (MQ=255)
                                     ttCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttca                                                                                                               <  2:1812082/150‑1 (MQ=255)
                                     taccGCTAAGCCTTCGCACATATCGGTAACTATCATGCCTCCTCTTATTCTTTCGCGCATGGGCCTCTGCAATGCGATCACTCCGGTCTGATAAGTGCGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttca                                                                                                               <  2:98024/148‑1 (MQ=255)
                                       ccGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttcatt                                                                                                             >  1:1009355/1‑150 (MQ=255)
                                          gTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCAc                                                                                                          >  2:1124414/1‑150 (MQ=255)
                                            aaGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCt                                                                                                        >  2:567057/1‑150 (MQ=255)
                                                     cacaTATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACgca                                                                                               >  1:187637/1‑150 (MQ=255)
                                                         tatCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGcagcag                                                                                           <  1:656004/150‑1 (MQ=255)
                                                         tatCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGcagcag                                                                                           >  2:1080762/1‑150 (MQ=255)
                                                         aatCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTTGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTACGCGACTTCATTCACCTGACGACGcagcag                                                                                           <  2:1633975/149‑1 (MQ=255)
                                                          atCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAgg                                                                                          >  1:1538875/1‑150 (MQ=255)
                                                          atCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAgg                                                                                          >  2:1233199/1‑150 (MQ=255)
                                                            cGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGa                                                                                        >  2:390526/1‑150 (MQ=255)
                                                            cGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGa                                                                                        >  1:1793842/1‑150 (MQ=255)
                                                              gTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGaaa                                                                                      <  1:564820/150‑1 (MQ=255)
                                                                   tAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCggg                                                                                 <  2:250920/150‑1 (MQ=255)
                                                                     gCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgggcc                                                                               <  1:1564794/150‑1 (MQ=255)
                                                                      cTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgggccg                                                                              >  2:703212/1‑150 (MQ=255)
                                                                        tGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCCGCAGGGAAAGCgggccggg                                                                            >  2:1682351/1‑150 (MQ=255)
                                                                         gCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGTgggccgggg                                                                           >  1:1434154/1‑150 (MQ=255)
                                                                          ccTGCTCTTATTCTTTCGGTCTTTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCTCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgggccggggc                                                                          <  1:974068/150‑1 (MQ=255)
                                                                          ccTGCTCTTATTCTTGCGGCCATTGGCATGTTCACTGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCGCGCGCCTCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgggccggggc                                                                          <  2:82918/150‑1 (MQ=255)
                                                                            tGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGATGACGCAGCAGGGAAAGCgggccggggccg                                                                        >  2:1334853/1‑150 (MQ=255)
                                                                             gCTCTTTTTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGc                                                                       >  2:602371/1‑150 (MQ=255)
                                                                              cactTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATACGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCATCAGGGAAAGCGTGCAGGGGCCGCt                                                                      >  2:1567750/3‑150 (MQ=255)
                                                                               tctTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATACGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTa                                                                     <  1:1016529/150‑1 (MQ=255)
                                                                                ctTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTaa                                                                    >  1:624334/1‑150 (MQ=255)
                                                                                 ttATTCTTTCGGTCATTGGCATGTTCAATGCGAGCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGCCCCGGCCCGCtttc                                                                   <  1:117877/150‑4 (MQ=255)
                                                                                  tATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGGTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGCCCCGGCCCGCtttcc                                                                  <  1:789559/150‑5 (MQ=255)
                                                                                  tATTCTTTCGGCCATTGTCATGTTCAATGCGATCACTCCGTTATGATATGTTGGGCGGATAAGGCGCTCGCGCCGCAGGCGACTTTGATTGCTTAAGCGACTTCATTCACCTGACGCCGCAGCAGGGAAAGCGGCCCCGGCCCGCtttcc                                                                  <  1:752185/150‑5 (MQ=255)
                                                                                     tCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTg                                                               >  1:1515696/1‑150 (MQ=255)
                                                                                     gcTTTCGGGCATTGCCAGGGTCAAGGCGAGCACTCCGTAATGATATGTTGGTCGGATCAGGCGCTCGCGCCGCAACCGACATTGATTGCTTACGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCATg                                                               <  1:321982/149‑1 (MQ=255)
                                                                                      tgttgcgTCATTTGGAAGTTTAATGGCTTCACCCCCTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGa                                                              <  2:816909/144‑1 (MQ=255)
                                                                                      cTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGa                                                              <  2:1650199/150‑1 (MQ=255)
                                                                                        ttCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAac                                                            <  1:1295512/150‑1 (MQ=255)
                                                                                        ttCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACAGTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAac                                                            <  2:1193118/150‑1 (MQ=255)
                                                                                          cGGTCATTGGCATGTTCAATGCGATAACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTAAAGCGACTTCATACACCTGCCGACGCAGCAGGGAAACCGGCCCGGGGCCGCTACGCGTGAacac                                                          >  2:270518/1‑150 (MQ=255)
                                                                                           ggTCACTGGCATGTTGACTGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACg                                                         <  1:204018/150‑1 (MQ=255)
                                                                                            gTCATTGGCATGTTCAATGCGATCACTCAGTTATGATATGTTGGTCGGATAAGGCGCTCGAGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACgg                                                        <  2:531218/150‑1 (MQ=255)
                                                                                                 tGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAAtt                                                   <  1:1659718/150‑1 (MQ=255)
                                                                                                     aTGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAgg                                               <  2:449873/150‑1 (MQ=255)
                                                                                                     aTGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAgg                                               <  1:1504759/150‑1 (MQ=255)
                                                                                                      tGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGt                                              <  2:1389133/150‑1 (MQ=255)
                                                                                                          cAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAg                                          <  1:1233199/150‑1 (MQ=255)
                                                                                                           aaTGCGATCACTCCGTTATGATATGTTGGGCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGc                                         <  2:690593/150‑1 (MQ=255)
                                                                                                             tGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGccc                                       >  1:453445/1‑150 (MQ=255)
                                                                                                                 aTCACTCCGTTATGATATGGTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAgcg                                   <  1:737305/150‑1 (MQ=255)
                                                                                                                  tCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGCCGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAgcgc                                  >  1:1775281/1‑150 (MQ=255)
                                                                                                                     caccGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccac                               <  2:125938/148‑1 (MQ=255)
                                                                                                                      tcagTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccacc                              <  1:1645132/147‑1 (MQ=255)
                                                                                                                       ccGTTATGATATGTTGGTCGGATAAGGCGCGCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccacca                             <  2:280066/150‑1 (MQ=255)
                                                                                                                         gTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGATATTAAGGTGAAGCCCAGCGCCACCAgc                           >  1:768733/1‑149 (MQ=255)
                                                                                                                           tATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAcc                         <  2:1368865/150‑1 (MQ=255)
                                                                                                                                aatcaatgTCGGATGCGGCGCGAGCGCCTTATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCa                    >  1:853566/8‑150 (MQ=38)
                                                                                                                                  agTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGACGGGGCCGCTAAGCGTGAACACTGAAATTAAGGAGAATCCCAGCGCCACCAGACCCAGCAcc                  >  2:1222186/2‑150 (MQ=255)
                                                                                                                                     tGGGCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGa               <  1:1292817/150‑1 (MQ=255)
                                                                                                                                     atgTCGGATGCGGCGCGAGCGCCTTATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGCGGCCGCTAAGCGTGAACACGGAAAATAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGa               >  1:1224299/3‑150 (MQ=255)
                                                                                                                                      ggTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGCACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGCGCCAGCCCCAGAt              >  1:354413/1‑150 (MQ=255)
                                                                                                                                      ggTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGAt              >  2:1059419/1‑150 (MQ=255)
                                                                                                                                      ggTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGAt              <  2:917978/150‑1 (MQ=255)
                                                                                                                                       gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATa             <  1:818299/150‑1 (MQ=255)
                                                                                                                                       gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATa             >  2:1530209/1‑150 (MQ=255)
                                                                                                                                       gTCGGATAAGGCGCTCGCGCCGCATCAGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATa             >  1:1025588/1‑150 (MQ=255)
                                                                                                                                         cGGATGCGGCGCGAGCGCCTTATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAg           <  2:853566/150‑1 (MQ=255)
                                                                                                                                         cGGATAAGGCTCTCGCGCCGCATCCGACATTGATTGCGTAAGCGACTTCATTCAACTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACAAGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAg           <  1:1123409/150‑1 (MQ=255)
                                                                                                                                              aaGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGccc      >  1:1080199/1‑150 (MQ=255)
                                                                                                                                                ggCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTg    <  1:108910/150‑1 (MQ=255)
                                                                                                                                                ggCGCTCGCGCCGCATCCGACATTGATTGCGTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCACAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTg    <  2:1351355/150‑1 (MQ=255)
                                                                                                                                                  cgcTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTggc  >  2:1511203/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGA  >  NC_000913/361752‑362047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: