Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,443 C→A 8.1% G70V (GGC→GTC) ‡ yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4430CA 8.1% 286.3 / 9.9 103G70V (GGC→GTC) ‡yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base C (53/41);  new base A (4/5);  total (57/46)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACT  >  NC_000913/3256294‑3256592
                                                                                                                                                     |                                                                                                                                                     
tCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGc                                                                                                                                                       >  1:397396/1‑150 (MQ=255)
 cTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGcc                                                                                                                                                      >  1:44446/1‑150 (MQ=255)
 cTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCAATCCCGTGcc                                                                                                                                                      >  2:762522/1‑150 (MQ=255)
 attAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGcc                                                                                                                                                      <  2:619278/149‑1 (MQ=255)
   taccGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCAGCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCTCAATCGGCAGCCCCACCATTCCCGGGCCgg                                                                                                                                                    <  1:302687/147‑1 (MQ=255)
   tAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCgg                                                                                                                                                    <  1:1696588/150‑1 (MQ=255)
      cGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGaa                                                                                                                                                 <  2:810918/150‑1 (MQ=255)
         agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACgg                                                                                                                                              <  2:578747/150‑1 (MQ=255)
          gaCTTTCCCCGCCGCCAGCAGAGCTTATGAATCGGCAATTGACTGCGCTGTAGCGTCTAAAAGCACTTCCACCCCGACGAAGGCAATAACACATAACGCAACAAGCGCCGCCGCAAACAGCATACAAACAATTCCCATGCCGGGAAAGGt                                                                                                                                             >  2:440314/1‑150 (MQ=255)
            cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTgg                                                                                                                                           >  1:775503/1‑149 (MQ=255)
            cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTACCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgg                                                                                                                                           <  1:959888/150‑2 (MQ=255)
             tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGGCTTTCAGCACATCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGAc                                                                                                                                          <  1:1368226/150‑1 (MQ=255)
             tttCCCCGCCGCCAGCAGGGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggg                                                                                                                                          <  1:154163/150‑3 (MQ=255)
              ttCCCCGCCGCCATGAGTGCTTTGTCATCGGCAATTGCCTGCGCTGTTGCGTCTTTAAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                                                                                         <  2:1816425/150‑1 (MQ=255)
              ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgggg                                                                                                                                         <  2:1812861/150‑4 (MQ=255)
              ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                                                                                         <  1:149834/150‑1 (MQ=255)
              ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                                                                                         >  1:1641392/1‑150 (MQ=255)
               tCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTTTTGCGTCTTTCATCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggc                                                                                                                                        <  2:591712/150‑5 (MQ=255)
               tCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGc                                                                                                                                        <  2:1504164/150‑1 (MQ=255)
               tCCCCGCCGCCAGCAGTGCTTTGGCAGCGGCAATTGCCTGCGCTGTTGCGGCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggc                                                                                                                                        <  1:644157/150‑5 (MQ=255)
                 cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                                      <  1:107658/150‑1 (MQ=255)
                 cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGAGACGccc                                                                                                                                      >  2:1735932/1‑150 (MQ=255)
                 cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGACTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCAGACGCAATCGTCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                                      >  1:229725/1‑150 (MQ=255)
                  ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCa                                                                                                                                     <  1:1296994/150‑1 (MQ=255)
                       gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGAcag                                                                                                                                >  2:1136809/1‑148 (MQ=255)
                           gcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttct                                                                                                                            <  1:1767584/150‑1 (MQ=255)
                             agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGttcttc                                                                                                                          >  2:1186815/1‑150 (MQ=255)
                                gCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatc                                                                                                                       >  2:60237/1‑150 (MQ=255)
                                gCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatc                                                                                                                       <  1:1623320/150‑1 (MQ=255)
                                gCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCACCCAGCGCCGCCGCAATCGGCAGCCCCACCATTACCGTGACGGGAACGGTGACGCCCAGACCGTTCTtcata                                                                                                                       >  2:18075/1‑149 (MQ=255)
                                 cTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGGTCTtcatca                                                                                                                      >  1:1727254/1‑150 (MQ=255)
                                  tttGGCATCGGCAATTGCCTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAg                                                                                                                     <  1:1158168/150‑1 (MQ=255)
                                     ggCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAtt                                                                                                                  >  1:1382985/1‑150 (MQ=255)
                                      gCATCGGCAATTGCCTGCGCCGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCAATCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAttt                                                                                                                 <  2:323928/150‑1 (MQ=255)
                                           ggCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGa                                                                                                            >  2:1084612/1‑150 (MQ=255)
                                                     tGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACCGTGACGCCCAGACCGTTCTTCATCAGAATTGGCGAAACCCAGGCt                                                                                                  >  1:1641603/1‑150 (MQ=255)
                                                        gcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCt                                                                                               >  2:487686/1‑150 (MQ=255)
                                                        gcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCt                                                                                               <  1:1561319/150‑1 (MQ=255)
                                                          tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                             <  2:483189/150‑1 (MQ=255)
                                                          tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                             >  1:251340/1‑150 (MQ=255)
                                                          tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                             >  1:246360/1‑150 (MQ=255)
                                                              gCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCGACAGATGTGGCGCAACCCAGGCTTCGACCCGt                                                                                         >  2:344010/1‑150 (MQ=255)
                                                                gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                       >  1:440191/1‑150 (MQ=255)
                                                                    ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAcc                                                                                   >  2:1328682/1‑150 (MQ=255)
                                                                      cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTTACGCCCCCAGCGCCGCCGCAATCGGCCCCCCCACCATTCCCGTCCCGGGAACGGTGACGCCCAGAGCGTTCTTCATCAGAGTTGGCGAAACCCAGGCTTCTACAGGTTCAACCgg                                                                                 >  2:1221680/1‑150 (MQ=255)
                                                                      cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                                 >  1:1294448/1‑150 (MQ=255)
                                                                        gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAc                                                                               >  1:348217/1‑150 (MQ=255)
                                                                         cACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAcc                                                                              >  2:443081/1‑150 (MQ=255)
                                                                          aCTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAccc                                                                             <  1:432924/150‑2 (MQ=255)
                                                                           cTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt                                                                            <  2:1593864/150‑1 (MQ=255)
                                                                             tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                          <  1:586363/150‑1 (MQ=255)
                                                                             tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                          <  1:1735932/150‑1 (MQ=255)
                                                                              ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCa                                                                         >  2:1578135/1‑150 (MQ=255)
                                                                                  cccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTc                                                                     <  1:436148/150‑1 (MQ=255)
                                                                                   ccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCCGCCCGTTCTTGAGCTGATTTGGCGAACCCCAGGCTTATACCCGGTCAACCGGACCTGCCAGGTCt                                                                    >  1:918897/1‑150 (MQ=255)
                                                                                   ccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGGTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCCACCGGCCCTTCCAGTTCt                                                                    >  1:1802187/1‑150 (MQ=255)
                                                                                     agCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGc                                                                  >  1:1019167/2‑150 (MQ=255)
                                                                                      gCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGcc                                                                 <  1:1084551/150‑1 (MQ=255)
                                                                                        gTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                               <  2:44446/150‑1 (MQ=255)
                                                                                        gTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                               <  2:474440/150‑1 (MQ=255)
                                                                                          tGGCATTTCCCCCTTAAGGCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcag                                                             <  1:754007/150‑1 (MQ=255)
                                                                                               tttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAct                                                        >  2:694784/1‑149 (MQ=255)
                                                                                                 tCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgc                                                      >  2:1564016/1‑150 (MQ=255)
                                                                                                   cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                    >  2:1131296/1‑150 (MQ=255)
                                                                                                   cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                    >  2:413736/1‑150 (MQ=255)
                                                                                                     ccTACCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAACGGTGCCGCCCAGGCCGTGCTACAGCAGATTTGGCGACACCCAGGCTTCGACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc                                                  >  1:18078/1‑150 (MQ=255)
                                                                                                          cGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTCCACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCgccgccgcccg                                             >  1:607648/1‑148 (MQ=255)
                                                                                                          cGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccag                                             >  2:1357765/1‑150 (MQ=255)
                                                                                                            cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                           >  1:1389810/1‑150 (MQ=255)
                                                                                                               ccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGACCGGTGACGCCCAGCCCGTTCTTAATCAGATTTGGCGCAACCCAGGCTTCTACACGTTCAACCGGCCGTTCCAGTTCTGCCGCAGCAACCGCCGCcgcccgcgccc                                        >  2:1412477/1‑149 (MQ=255)
                                                                                                                  gcgcCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGTACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTATACACGTTCAACCGTACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGcccgtgcccgtg                                     >  2:47917/1‑150 (MQ=255)
                                                                                                                   cgccgccgcAATCGTCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGa                                    <  2:795808/150‑1 (MQ=255)
                                                                                                                   cgccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGa                                    <  1:747942/150‑1 (MQ=255)
                                                                                                                    gccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCAACCGCCGCCGCCAGCGCCAGTGaa                                   >  2:1259264/1‑150 (MQ=255)
                                                                                                                       gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                                >  1:1712210/1‑150 (MQ=255)
                                                                                                                        ccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg                               >  1:762689/1‑150 (MQ=255)
                                                                                                                         cgcAATGGGCAGCCGCACCTTTCCCGTGCCGGGACAGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGACCCCCAGGCTTCTACACGTTCCACCGGACCTTCCCGGTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                              <  1:290880/150‑1 (MQ=255)
                                                                                                                         cgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                              <  1:60237/150‑1 (MQ=255)
                                                                                                                         cgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                              >  2:12484/1‑150 (MQ=255)
                                                                                                                           cAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTACCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt                            <  1:1084612/150‑1 (MQ=255)
                                                                                                                            aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTc                           <  1:553017/150‑1 (MQ=255)
                                                                                                                             aTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTGCATCAGATTAGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCACCCGCCGCCGCCAGCGCCCGTGCAAGCGGtgcc                          >  1:1165047/1‑147 (MQ=255)
                                                                                                                              tCGGCAGCCCCACCATTCCCGTGCCGGTAAGGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCTAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAg                         <  2:685167/150‑1 (MQ=255)
                                                                                                                              tCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACTGCCGCCGCCAGCGCCAGTGAAATCGGTTCAg                         <  1:694784/150‑1 (MQ=255)
                                                                                                                              tCGGCAGCCCAACAATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCTTATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAg                         <  2:341218/150‑1 (MQ=255)
                                                                                                                               cGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGt                        >  2:519513/1‑150 (MQ=255)
                                                                                                                                    gCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATc                   >  2:426849/1‑150 (MQ=255)
                                                                                                                                     ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCCGGCTTCTTCACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc                  >  1:972166/1‑150 (MQ=255)
                                                                                                                                     ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc                  >  2:68644/1‑150 (MQ=255)
                                                                                                                                      cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCCTCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCCACCGCCGCCGCCAGCGCCAGTGAAATCGGATCAGTAAATccc                 >  2:326168/1‑150 (MQ=255)
                                                                                                                                       tcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcccc                >  1:396902/2‑149 (MQ=255)
                                                                                                                                       tcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCa                >  2:164044/2‑150 (MQ=255)
                                                                                                                                       ccaccaTTCCCGTGCCGCGACCGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGCCTTCTACACGTTCAACCCGACCTTCCAGTTCTGCCGCAGAAACCGCCGCCGCCAGCGCCAGTGAAATAGGTTCAGTACATCCCa                >  2:522913/1‑150 (MQ=255)
                                                                                                                                        caccgTTCCCGGCACGGGAATGGTGACGACCAAACCGTTCATCATCAGAATTGACTAAAACCAGGCTTATACAAGTTAAAAAGGCCATTCCATATATTAAGAAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg               >  2:198698/1‑150 (MQ=255)
                                                                                                                                               cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt        >  2:1037036/1‑150 (MQ=255)
                                                                                                                                               cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCAACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt        <  1:1221680/150‑1 (MQ=255)
                                                                                                                                                ccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtt       >  1:796828/1‑150 (MQ=255)
                                                                                                                                                 ggTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGttt      <  1:1136809/149‑1 (MQ=255)
                                                                                                                                                    gCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTAc   <  1:576628/150‑1 (MQ=255)
                                                                                                                                                     ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt  >  1:1603945/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACT  >  NC_000913/3256294‑3256592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: